LOCUS       NZ_AP027617             1896 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-72 plasmid pLR-72_5, complete sequence.
ACCESSION   NZ_AP027617
VERSION     NZ_AP027617.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562557
            Assembly: GCF_036498665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 162x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036498665.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:14:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,769
            CDSs (total)                      :: 4,647
            Genes (coding)                    :: 4,428
            CDSs (with protein)               :: 4,428
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 73 of 219
            Pseudo Genes (incomplete)         :: 144 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 39 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027617.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-72"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-72_5"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     misc_feature    6..1896
                     /note="23S ribosomal RNA rRNA prediction is too short"
ORIGIN
        1 tgtatagtca agtaattaag tgcatgtggt ggatgccttg gcagtcagag gcgatgaaag
       61 acgtgatagc ctgcgaaaag ctccggggag gcggcaaata tcctttgatc cggagatgtc
      121 tgaatggggg aacccaccta ctttaaggta ggtattgcaa catgaataca tagtgttgca
      181 aggcgaacga ggggaagtga aacatctcag tacccttagg aaaagaaatc aattgagatt
      241 ccctcagtag cggcgagcga acggggatca gcccattaag ttatgtgtgt tttagtggaa
      301 cgctctggga agtgcgaacg tagagggtga tattcccgta cacgaaaggg cacacataat
      361 gatgacgagt agggcgaggc acgtgaaacc ttgtctgaat atggggggac catcctccaa
      421 ggctaaatac tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa
      481 cccctgtgag gggagtgaaa tagatcctga aaccgcatgc atacaagcag tgggagcacc
      541 tttgtggtgt gactgcgtac cttttgtata atgggtcagc gacttatatt cagtagcgag
      601 gttaaccgta taggggagcc gtagagaaat cgagtcttaa tagggcgttt agttgctggg
      661 tatagacccg aaaccaggcg atctatccat gagcaggttg aaggttgggt aacactaact
      721 ggaggaccga acccactgtc gttgaaaagc caggggatga cttgtggata ggggtgaaag
      781 gctaatcaag cctggtgata gctggttctc cccgaaagct atttaggtag cgcctcggac
      841 gaataccata gggggtagag cactgtttcg gctagggggt catcccgact taccaaaccg
      901 atgcaaactc cgaataccta tgagtactat ccgggagaca gactgcgggt gctaacgtcc
      961 gtagtcaaga ggaaaacaat ccagaccgcc agctaaggcc ccaaaatcat agttaagtgg
     1021 gaaacgatgt gggaaggcat agacagctag gaggttggct tagaagcagc caccctttaa
     1081 agaaagcgta atagctcact agtcgagtcg gcctgcgcgg aagatgtaac ggggctaaaa
     1141 ctatgtgccg aagctgcgga tgtatacttt gtatacgtgg taggggagcg ttctgtaagc
     1201 cgatgaaggt gtgttgagaa gcatgctgga ggtatcagaa gtgcgaatgc tgacgtgagt
     1261 aacgacaaaa cgggtgaaaa acccgttcgc cgaaagacca agggttccag tccaacgtta
     1321 atcggggctg ggtgagtcga cccctaaggc gaggccgaaa ggcgtagtcg atgggaaaat
     1381 ggttaatatt ccattacttc tgtgtaatgc gatgagagga cggagaaggc taaatcagcc
     1441 tggcgttggt tgtccaggtg aaaggatgta ggcatgtatc ttaggcaaat ccggggtact
     1501 ctatgctgag atctgatagc aagctgtact tgtacagcga agtggttgat gccatgcttc
     1561 caggaaaagt ctctaagctt cagttacaca ggaatcgtac ccgaaaccga cacaggtggt
     1621 caggtcgagt agaccaaggc gcttgagaga actctgctga aggaactagg caaaatggta
     1681 ccgtaacttc gggagaaggt acgctgttgt tggtgatgga actcgcttcc tgagctgacg
     1741 acagccgcag aaaccaggcc gctgcaactg tttattaaaa acatagcact ctgcaaacac
     1801 gaaagtggac gtatagggtg tgatgcctgc ccggtgctgg aaggttaatt gatggggtta
     1861 gcgcaagcga agctcttgat cgaagccccg gtaaac
//
