LOCUS       NZ_AP027616             2911 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-72 plasmid pLR-72_4, complete sequence.
ACCESSION   NZ_AP027616
VERSION     NZ_AP027616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562557
            Assembly: GCF_036498665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 162x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036498665.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:14:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,769
            CDSs (total)                      :: 4,647
            Genes (coding)                    :: 4,428
            CDSs (with protein)               :: 4,428
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 73 of 219
            Pseudo Genes (incomplete)         :: 144 of 219
            Pseudo Genes (internal stop)      :: 42 of 219
            Pseudo Genes (multiple problems)  :: 39 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027616.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-72"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-72_4"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            complement(1..2840)
                     /locus_tag="AABJ55_RS23835"
     rRNA            complement(1..2840)
                     /locus_tag="AABJ55_RS23835"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
                     /name="23S ribosomal RNA"
ORIGIN
        1 atccattgct gaacttccac atagcgccta ttaacgggtg ttctacccgt gaccttactg
       61 gcttatgcca tgagagtact catcttgagg tgggcttccc acttagatgc tttcagcggt
      121 tatccactcc acacgtagct acccagcgtt taccgttggc acgataactg gtacaccagc
      181 ggtgcgttcc tcccggtcct ctcgtactag ggagaaatcc tctcaatact cttacgcctg
      241 caccggatat ggaccgaact gtctcacgac gttctgaacc cagctcacgt accgctttaa
      301 tgggcgaaca gcccaaccct tgggaccgac ttcagcccca ggttgcgatg agccgacatc
      361 gaggtgccaa acctcctcgt cgatgtgaac tcttggagga gatcagcctg ttatccctag
      421 agtaactttt atccgttgag cgacggccct tccactcaga accgtcggat cactaaggcc
      481 gactttcgtc cctgctcgac ttgtaggtct tgcagtcaag ctcccttatg cctttgcact
      541 cgtcggctga tttccaacca gcctgaggga acctttgcgc gcctccgtta ccttttagga
      601 ggcgaccgcc ccagtcaaac tgcccacctg atactgtcct tcggccggat aacggctcaa
      661 agttagactt ctagctttga cagagtggta tctcaccgtt ggctccactt cccccacaag
      721 gaaagtctca tagcctccca cctatcctgc gcagtcaaag cccgaagcca ataccaagct
      781 acagtaaagc ttcatagggt ctttctgtcc gggtgcaggt agtccgtatc ttcacagaca
      841 ctcctatttc gccgagcctc tctctgagac agcgcccaga tcgttacgcc tttcgtgcgg
      901 gtcggaactt acccgacaag gaatttcgct accttaggac cgttatagtt acggccgccg
      961 ttcaccgggg cttcggtcgc cagcttcact tgcgctgacc agcttcctta accttccggc
     1021 actgggcagg cgtcagcccc catacatcgt cttacgactt agcggagacc tgtgtttttg
     1081 gtaaacagtc gcctgggcct cttcactgcg acccacgtct taggtgggca ctccttctcc
     1141 cgaagttacg gagtcatttt gccgagttcc ttagagagag ttacctcgcg ccccttggta
     1201 tactctaccg tcccacctgt gtcggtttag ggtacaggca attttggatt aacgtgttta
     1261 gagcttttct tggaagcttg acatcaacca cttccccgcc gtagcgggtc gcactcacgc
     1321 ctcagctcgg agcgttttcg ccgctcctca acgcctcgga cacttggaca ggtaaccaac
     1381 atcctgctgg cctagccttc tccgtccctc tgcacaatcc aaaatcggta caggaatatt
     1441 aacctgttgt ccatcgacta cgcacttctg cctcgcctta ggacctgact aaccctccgc
     1501 ggacgagcct tccggaggaa cccttgggat ttcggggcat gggattctca cccatgtttt
     1561 cgctactcaa gccgacattc tcacttccgt ttcgtccacg gctgcttccg ctaccgcttc
     1621 accctacgac ggaacgctcc cctaccaatt gcacagcaat tccacagctt cggtacagaa
     1681 cttagccccg ttcattttcg gcgcaggagc gcttgaccag tgagctatta cgcactcttt
     1741 taaggatggc tgcttctagg caaacctcct ggttgtctat gcactcccac ctcctttatc
     1801 actaagttct gatttgagga ccttagctgg tggtctgggc tgtttccctc ttgaccatgg
     1861 agcttatccc ccacagtctg actggcatgg tgtacacggc gtattcggag tttgactcga
     1921 tttggtaccg ctctcgcagc ccgcaccgaa tcagtgctct accccaccgc tataatccat
     1981 accgctacgc ctcaacgtat ttcggggaga accagctagc tccgggttcg attggcattt
     2041 cacccctaac cacagctcat ccgccgattt ttcaacatcg gtcggttcgg acctccactt
     2101 ggtgttaccc aagcttcatc ctggccatgg ttagatcacc cgggttcggg tctataaaca
     2161 ctgactatcg ccctattcag actcgctttc gctttggctt cggcatttcc gccttaacct
     2221 gccagtgcct ataagtcgcc ggctcattct tcaacaggca cgccgtcacc cgttgaatcg
     2281 ggctccgact gcttgtaagc tcacggtttc atgttctatt tcactcccct tccggggttc
     2341 ttttcacctt tccctcgcgg tactggttca ctatcggtca cacaggagta tttagcctta
     2401 cgaggtggtc ctcgcggatt cacacggaat ttcacgtgct ccgtgctact cgggattcag
     2461 ctaggctgtt tcaattttcg actacaggac tttcaccttc tctggtgcag ttttcagctg
     2521 cttcgtctaa cattcacagt ccacattgct gtcccacgac cccaaactcc gtggagtttg
     2581 gtttaggctg gtccccgttc gctcgccgct actgagggaa tcacttttgt tttctcttcc
     2641 tccggctact aagatgtttc aattcgccgg gttcgctcgc gccaccctat ggattcaggt
     2701 ggccgtacat ggggttgccc cattcggaaa tcctcggatc aatgcttgct tccagctccc
     2761 cgaggcgtat cgtcggtagc cacgtccttc ttcgcctctg tgtgcctagg tatccaccgt
     2821 aagcccttcg tagcttgacc actacttctt tctgacttcc atttctccca cttgactcaa
     2881 tcaagtgatg atgaagatgg tgtctgtgaa g
//
