LOCUS       NZ_AP027605             5892 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-71-1 plasmid pLR-71-1_6, complete
            sequence.
ACCESSION   NZ_AP027605
VERSION     NZ_AP027605.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562543
            Assembly: GCF_036498465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 84x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036498465.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:05:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,144
            CDSs (total)                      :: 5,026
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 78 of 284
            Pseudo Genes (incomplete)         :: 199 of 284
            Pseudo Genes (internal stop)      :: 46 of 284
            Pseudo Genes (multiple problems)  :: 38 of 284
            Pseudo Genes (short protein)      :: 3 of 284
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027605.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-71-1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-71-1_6"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            join(5715..5892,1..161)
                     /locus_tag="AABJ98_RS25555"
     CDS             join(5715..5892,1..161)
                     /locus_tag="AABJ98_RS25555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_338384947.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCDGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVR
                     EQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            370..>801
                     /locus_tag="AABJ98_RS25560"
                     /pseudo=""
     CDS             370..>801
                     /locus_tag="AABJ98_RS25560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            839..1357
                     /locus_tag="AABJ98_RS25565"
                     /old_locus_tag="VEE28_49280"
     CDS             839..1357
                     /locus_tag="AABJ98_RS25565"
                     /old_locus_tag="VEE28_49280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067499.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTVSTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEMIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1364..1597
                     /locus_tag="AABJ98_RS25570"
                     /old_locus_tag="VEE28_49290"
     CDS             1364..1597
                     /locus_tag="AABJ98_RS25570"
                     /old_locus_tag="VEE28_49290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1628..1789
                     /locus_tag="AABJ98_RS25575"
     CDS             1628..1789
                     /locus_tag="AABJ98_RS25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_071779130.1"
                     /translation="MRYPWNGSVSWKYRNAHWMALRWDGETMKNGRFIGLRRTNVLCAI
                     WLHRYSYG"
                     /name="mobilization protein"
     gene            complement(1932..2816)
                     /locus_tag="AABJ98_RS25580"
                     /old_locus_tag="VEE28_49300"
     CDS             complement(1932..2816)
                     /locus_tag="AABJ98_RS25580"
                     /old_locus_tag="VEE28_49300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439342.1"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="WP_021551692.1"
                     /translation="MENNTQGIKLIKEAVDVLPKEIRKSVYNKIRNVINYEPVIGIMGK
                     TGAGKSSLCNSIFKGNVCAVSDVEACTRKVQELIISFGDRTIKIIDIPGVGENEAKDIE
                     YEELYKNLLPELDLVLWVVKGDDRAFSADEHFYKNVLKPVGGDEKVLFVLNQVDKIEPF
                     REWDEEKHQPSPSQQINIRKKEEYLIERFGFTKHPVISVSANEGYNISRLVEAIVRSLP
                     KEAQSGVVHQVKDEIKTKEIVETAKDGFADTVDRAIDDIIDTVLPKPIASVAKVAKKAI
                     VSVAKSVWGLLFG"
                     /name="GTPase"
     gene            complement(2852..3286)
                     /locus_tag="AABJ98_RS25585"
                     /old_locus_tag="VEE28_49310"
     CDS             complement(2852..3286)
                     /locus_tag="AABJ98_RS25585"
                     /old_locus_tag="VEE28_49310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021551691.1"
                     /translation="MGFWDSIKNAAIKAKCGVGIHGGNYKLIDGETCKYSKLCPDCNRT
                     IQKEQHKYGEESYKYDFKCTTVKKCIDCGAEQEGERHERFVEIAVDDYCNVKERCVRCF
                     TERVHGKRHNWYLSGSSDTYRHYKCSVCGEEKEERKTSFR"
                     /name="hypothetical protein"
     gene            complement(3514..4326)
                     /locus_tag="AABJ98_RS25590"
                     /old_locus_tag="VEE28_49320"
     CDS             complement(3514..4326)
                     /locus_tag="AABJ98_RS25590"
                     /old_locus_tag="VEE28_49320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="WP_000549944.1"
                     /translation="MHLEEGDLRALERAVELLEQDTITETLTQLVGTPIDYLMSKLPKG
                     AEEQIYSLVEKSLHKAADAALWSLDNEPNRIASTKTNKFFAAVSGAIGGAFGFSALAIE
                     LPVSTTIMLRSIADIARSEGFELDNIDTKLACLEVFSLGGPSDDDDAANTAYYATRSLT
                     AEIVQDLSKEISNIAVKNASSVQTGKWLASLIEKVATRFGIVITEKMAAQVVPVIGALT
                     GATLNTMFTDYYQDMARGHFIVKRLEKKYGNELIKAEYIKISSKLTNS"
                     /name="EcsC family protein"
     gene            complement(4645..4803)
                     /locus_tag="AABJ98_RS25595"
     CDS             complement(4645..4803)
                     /locus_tag="AABJ98_RS25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(5474..5665)
                     /locus_tag="AABJ98_RS25600"
                     /old_locus_tag="VEE28_49330"
     CDS             complement(5474..5665)
                     /locus_tag="AABJ98_RS25600"
                     /old_locus_tag="VEE28_49330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165993.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHEHA
                     DELYRSCLSRFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5301..5385
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="99.0"
                     /evalue="2.36e-33"
     Typing          complement(4732..5275)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.505"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          152..1701
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5701..5891
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.875"
                     /coverage="98.0"
                     /evalue="3.05e-33"
ORIGIN
        1 ccatcgggaa taacctgaat cagacggcga ggaaggtgaa cagcgggcag tggtcttccg
       61 gtgaccgggt tcaggtggtg gccgcactga tggccatcga gagagagttg cgcagcttac
      121 ggcaggtcgt cagggaacag ggagcgcggg atgatagtta aatttcatgc caggggaaaa
      181 ggcggtggca gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca
      241 acggtgcttc agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg
      301 aagaaataca cgtcaggtgt tctgtcgttc gcggagaagg agctgccgcc gggaggacgt
      361 gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac
      421 agcatcctgt gggttgagca ccaggacaag ggacggcttg agctgaattt tgtcattccg
      481 aacatggagc tacagagcgg aaaacgcctc cagccgtact atgaccgcgc agacaggccg
      541 agaattgatg cctggcagac gctggtgaat caccattacg ggctgcatga cccgaatgcc
      601 ccggagaacc gcaggacgct gacactccct gataacctgc cggaaacgaa acaggcgatt
      661 gctgagagcg ttacacgggg gcttgatgtg ctttaccgtg ccggagagat aaaagaccgt
      721 caggatgtga ttcaggcact cacagatgtg ggagttgagg ttgtcagggt gacgcgaagc
      781 agtatcagca ttgcagaccc gaacggcgga aggaatatca ggctgaaagg agcattttat
      841 gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc
      901 tacagagaaa atgctgaacg acgagttcag gaagctcggc gagtctgtca gcgaggctgt
      961 gacatcaaac gagacgaaaa tcagagacgc tatagccctg ttcacagtct cgacagagaa
     1021 atcgctggaa aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt
     1081 gttaaagctg gcagggaata cgggcatgat gttactgggg atagtctttc ttctgtttac
     1141 cgcgagtggc gggacgctct ggtatcttgg cggacggata cagacgaacc tggaagaaat
     1201 caggaagcag gaagagacat tgcagaaact gaacgcgaag acatggggcg tggagtttgt
     1261 gcaggacggg aacaggaaat ttctggtcct tccgtacggg aaatcagcag agatgattcc
     1321 ctttcagggg aaagagtggg tacatctgaa ggagtgacac agagtgacag agctggaaac
     1381 acatttgctg aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg
     1441 ggagagcgcc ttcgtggaat tgcagaagat gttttcgctt acgcaacggg acaacgcgat
     1501 gctgaacgag cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga
     1561 acgcttgagc cagttataca gcgagaactg gagataagag aggaacggtt gatgcaggag
     1621 cgcgaacatg cgttatccct ggaacgggag cgtcagctgg aaatacagga acgcacactg
     1681 gatggccctt cgctgggatg gtgaaaccat gaaaaatggc aggttcattg gattaaggcg
     1741 gactaatgtg ctctgtgcca tctggttgca taggtattca tacggttaaa atttatcagg
     1801 cgcgatcgtg gcagtttttc gggtggtttg ttgctatttt tacctgtctg ctgccgtgat
     1861 cgcgctgaac gcgttttagc ggtacgtaca aactgttgtt atgttaaata ctgatgggct
     1921 ttcctgcttg attatccgaa gagcaatccc caaacacttt tagctacaga aacaatggct
     1981 ttcttggcga ctttagccac tgacgctatt ggtttaggta gtacggtgtc tattatatca
     2041 tcaatagctc tatctacggt gtctgcgaac ccgtcctttg ccgtttctac aatttctttt
     2101 gtttttattt cgtcttttac ttgatgcacc actccgctct gtgcttcctt tgggagcgat
     2161 cttactattg cctccactaa ccttgaaatg ttataacctt cattcgcaga tacagatata
     2221 acgggatgtt tggtaaaacc gaatcgttct attaagtatt cttccttttt tctaatattt
     2281 atttgttgtg agggtgatgg ttggtgtttt tcttcatccc attcgcggaa tggctcaatc
     2341 ttgtcgactt ggtttaaaac aaacaacact ttttcatcac caccaactgg ttttaaaaca
     2401 tttttataaa agtgctcatc agctgaaaat gctcgatcat cacctttaac aacccaaagc
     2461 accagatcta attctgggag aagattttta tacaactctt catattctat atctttagct
     2521 tcattttcac ctacacctgg tatatctatt attttaattg ttctatcacc aaatgaaatt
     2581 attaattcct gaacctttct cgtgcatgct tctacatcac tcactgcaca tacattacct
     2641 ttaaagatag aattacatag gctggattta ccagcgccgg ttttacccat aatccctatt
     2701 actggttcgt aattaataac atttctaatt ttgttataga cagacttgcg aatctcttta
     2761 ggtagaacat ccacagcttc tttgattaat tttatacctt gcgtattgtt ttccatagtt
     2821 actattttcc tcattttgtt gttcatatga attaacggaa gcttgttttt ctttcttctt
     2881 tttcttcccc gcatacagaa catttgtaat ggcgataggt atcagaactc ccactaagat
     2941 accaattgtg tcttttacca tgaactcgct ctgtaaaaca tctcacgcat ctttctttca
     3001 cattgcaata atcatcgact gcaatttcaa caaatctttc atgcctttcg ccttcttgtt
     3061 cagcgccaca gtcgatacac ttttttacag ttgtgcactt gaaatcgtat ttataacttt
     3121 cctctccgta tttatgctgt tctttttgta tagtgcgatt gcagtcaggg catagtttag
     3181 aatatttaca agtctcacca tcaataagct tatagtttcc tccgtgtata cctaccccac
     3241 acttagcctt gattgctgca ttttttatgc tatcccaaaa gcccatggaa aaatctccct
     3301 acgattagtt gatataagtt gaacaattca tcgacacgat tatacacgta tagtgtgtgg
     3361 atatataaaa atgagtatgg atttatgctc tgtataatgt ttttgttatg ttaaatatag
     3421 cgctgaaaaa gtgggggcac tggataaact tcgtgttttt cccgcaaaga tagtattgga
     3481 aagtcattgt ctaattcatc caagataaat attttacgaa tttgtgagtt tgcttgagat
     3541 ttttatatat tcagccttta ttaactcatt gccatatttt ttctcgagtc ttttaacaat
     3601 gaaatgacct cgagccatat cttgataata gtcggtaaac attgtgttta atgtggctcc
     3661 agttagtgct ccaataacgg gaacgacttg agcagccatt ttttccgtaa taactatacc
     3721 aaatcttgtt gcaacttttt ctattaacga agctaaccat tttcctgttt gtactgaaga
     3781 tgcgtttttg acagcaatgt tagaaatttc tttggataaa tcttgtacga tctcggcagt
     3841 caaacttctt gttgcatagt atgcagtatt agctgcatca tcgtcatcag atggtcctcc
     3901 caaggaaaac acctctaagc aggccagttt ggtatcaata ttatctagct caaaaccttc
     3961 acttcttgct atgtcagcaa ttgaacgaag cataattgtc gttgaaaccg gtaactcaat
     4021 tgctaatgca gaaaagccaa aagcgccacc aatggctcca gatacggccg caaagaattt
     4081 atttgtctta gttgatgcta ttcggttagg ttcattgtct agactccata aggccgcatc
     4141 agcagctttg tggagcgatt tttctactag actataaatt tgctcttctg cccctttggg
     4201 aagtttactc attaagtaat ctattggggt tccaactaat tgggttaatg tttcagtaat
     4261 tgtatcttgt tctaataatt ctacggccct ttctaaagcc cgcagatctc cttcttcaag
     4321 atgcatataa tgaatccttt atgaatcaat cgatagtcta cttttgtaag tgattcgtct
     4381 tgggagctta ccttgagcga gttcagtgat tcaataggcg agatgccgac atcattgttg
     4441 tctgtgaagc aggagtgatg aaaagatcaa aggatcctct tgtgatcctt tttttctgcg
     4501 cgtaatctgc tgcctgtaaa caaaaaaacc accgctacca acggtggttt gtttgccgga
     4561 tcaagagcta ccaactcttt ttccgaaggt aactggcttg gtggagcaca gataccaaat
     4621 actgtccttc tagtgtagcc gcagttaggc caccacttca agaactctta atatctcaat
     4681 ccaccttgtc cagttaccag tggctgctgc cagtggcgct ttgtcgtgtc ttaccgggtt
     4741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4801 cacacagccc agcttggagc gaacgaccta caccgggccg agataccaac agcgtgagct
     4861 atgagaaagc gctacgcttc ccgaagggag aaaggcggac aggtatccgg taaacggcag
     4921 ggtcggaaca ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag
     4981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5041 gcggagccta tggaaaaacg ccagcggcgc ggcctttcgt tgccctttgc ctttttgctc
     5101 acatgttctt tccggcctta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5161 gagccggcac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag
     5221 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacatcgta
     5281 tatggcgctg ttttcttaca atccgcacta aatgccgcat agttaagcca gtatacactc
     5341 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgcaaaaa cccgctgacg
     5401 cgccctgacg ggcttgtctg ctcccggcat ccgctcaccg gcaagctgtg accgtctccg
     5461 ggagctacat gtgttacagg ttttcaccgt catccccgaa gcgtgagagg cagctgcggt
     5521 aaagctcatc ggcgtgctcg tgaagagatt cacagatgtc tgcctgttca tccgcgtcca
     5581 gttcgttgag cttctccagt agcgttaatg tctggcttct gataaagcgg gccatgttaa
     5641 gggcggtttt ttcctgttta gtcactgatg cctccgtgta agggggattt ctgttttatg
     5701 gggtaatgat accgatgaaa cgcgagagga tgctcaccat acgggttact gatgatgaac
     5761 atgcccggtt actggaacgc tgtgatggta aacaactggc ggtatggatg cggcgggtat
     5821 gcctgggtga gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc
     5881 gtcagctggc tg
//
