LOCUS       NZ_AP027604             9030 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-71-1 plasmid pLR-71-1_5, complete
            sequence.
ACCESSION   NZ_AP027604
VERSION     NZ_AP027604.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562543
            Assembly: GCF_036498465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 84x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036498465.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:05:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,144
            CDSs (total)                      :: 5,026
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 78 of 284
            Pseudo Genes (incomplete)         :: 199 of 284
            Pseudo Genes (internal stop)      :: 46 of 284
            Pseudo Genes (multiple problems)  :: 38 of 284
            Pseudo Genes (short protein)      :: 3 of 284
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027604.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-71-1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-71-1_5"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            262..453
                     /locus_tag="AABJ98_RS25495"
                     /old_locus_tag="VEE28_49170"
     CDS             262..453
                     /locus_tag="AABJ98_RS25495"
                     /old_locus_tag="VEE28_49170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1259..1552
                     /locus_tag="AABJ98_RS25500"
     CDS             1259..1552
                     /locus_tag="AABJ98_RS25500"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123874500.1"
                     /translation="MFEEQRFLAGEKQFCKRWYIDMSSFGINPGKTPGNKRNIPDVTYF
                     HFCQQHPPPTPENKKAACSIGKPYIKGNYVLRFVLNRDALLLRITNQIIHVL"
                     /name="hypothetical protein"
     gene            complement(1666..2363)
                     /locus_tag="AABJ98_RS25505"
                     /old_locus_tag="VEE28_49180"
     CDS             complement(join(1666..2114,2114..2363))
                     /locus_tag="AABJ98_RS25505"
                     /old_locus_tag="VEE28_49180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            <2418..2525
                     /locus_tag="AABJ98_RS25510"
                     /pseudo=""
     CDS             <2418..2525
                     /locus_tag="AABJ98_RS25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     gene            2900..3460
                     /locus_tag="AABJ98_RS25515"
                     /old_locus_tag="VEE28_49200"
     CDS             2900..3460
                     /locus_tag="AABJ98_RS25515"
                     /old_locus_tag="VEE28_49200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            3464..>3835
                     /locus_tag="AABJ98_RS25520"
                     /pseudo=""
     CDS             3464..>3835
                     /locus_tag="AABJ98_RS25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            3815..4060
                     /locus_tag="AABJ98_RS25525"
     CDS             3815..4060
                     /locus_tag="AABJ98_RS25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(4092..4742)
                     /gene="tetR(A)"
                     /locus_tag="AABJ98_RS25530"
                     /old_locus_tag="VEE28_49220"
     CDS             complement(4092..4742)
                     /gene="tetR(A)"
                     /locus_tag="AABJ98_RS25530"
                     /old_locus_tag="VEE28_49220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             4774..6047
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.57"
                     /coverage="100.0"
     ARG             4849..6044
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(6079..6963)
                     /locus_tag="AABJ98_RS25540"
                     /old_locus_tag="VEE28_49240"
     CDS             complement(6079..6963)
                     /locus_tag="AABJ98_RS25540"
                     /old_locus_tag="VEE28_49240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(7101..7493)
                     /locus_tag="AABJ98_RS25545"
     CDS             complement(7101..7493)
                     /locus_tag="AABJ98_RS25545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <7497..8361
                     /locus_tag="AABJ98_RS25550"
                     /pseudo=""
     CDS             <7497..8361
                     /locus_tag="AABJ98_RS25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     Typing          complement(8530..9030)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.051"
                     /coverage="95.0"
                     /evalue="4.79e-148"
ORIGIN
        1 tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg
       61 ggggcggagc ctatggaaaa acgccccgct ggggcgtggc cttttcgccc ttgtccttag
      121 ctttcgttat gcttaccgtt acgttatctg cttatcttcc ctccgctcgc cgcagactca
      181 tgaccgaacg gagtgagcga atgagcgagg aagcggaaaa ccatcagaac gtacagtaag
      241 cactttcgcg ccggggcctt catgaacaaa caacaaaaga cagccgttaa catggctaaa
      301 ttcatccagg atcagtccct gcttcttctg gacaggctta atgagctgga tctggatcat
      361 gaggctgatt tctgcgagaa actccatgag gatgcggaac gcctttaccg aagcctgtca
      421 gcgcgcctga atgagcagca ggacggtgcg taatggctcc gttgtgggta ttaccggagt
      481 aataccggca tcgccttaat tttcattgtc ttggtgctta atattattac cccggtaata
      541 cccagaatcc ctttattttt tcttttttat ttatttttca tgaagttgcg atgaaaatcg
      601 cagctgcgtt aggtgtatgt cattttttag tatataaaac gaccactccc gagtgctggc
      661 gccgcaggtt aaggatgatt ctggctaaaa agcgtccttt tccgggaaag ggtcatgctc
      721 gtatgaccct atgggttcag cgactacaca taaactacaa aattgcatct gcttttaccc
      781 cagaacgcgg gtgtacaaca gaattacatc aaaagtacat cacttttacg tcacttttac
      841 atcattttta cgtcagatct gacctctgaa ctattcgtac atccgccccc tcttcctggg
      901 cctcctgcac gtgctgaaaa tggtttttcc ggctgtgccg gcaacgcctc acggccacgc
      961 aataaattgc gcatccgttc accgttatcg gggtggttca tggtggcttt cagggcgttc
     1021 atcggatttt agccgctaaa atcccggcca ggtatgtccg tgcatagcca tgcagtcgcg
     1081 ccagaatcat caggggcgtt ttttctcgat atgggcacag aaaggtgtgt gtttcaggcc
     1141 ggaggcctca gaaacgccct gagagcctct cagacggtgc acgcaacgga tgttatggta
     1201 aatccggtgg ctttgacgtg cctgccgttg tgtaccgtcg tgagtgaaaa taattatatt
     1261 gttcgaagaa cagcgctttt tggccggaga aaagcagttc tgcaaacgat ggtatatcga
     1321 tatgagttct tttggcatta atccaggcaa gacgccgggc aataagcgta atattccgga
     1381 tgttacttat ttccatttct gtcaacagca tccccccccc acgccggaga ataaaaaagc
     1441 cgcctgttcc atcggcaagc cctacatcaa gggaaactac gtcttacgat tcgttttgaa
     1501 ccgggacgct ttgttgctgc gaatcactaa ccagataatc cacgttttgt gagtgggttt
     1561 aggttaaggc ttcaatagaa caataatagc gttaagtgat tagggcaacc tgtgattgct
     1621 aaaatgatga agtcttcaca tcatttcggt ggtaatgact ccaacttact gatagtgttt
     1681 tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag
     1741 cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg
     1801 ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg
     1861 ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc
     1921 cagcgtcgcc atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagcctgtc
     1981 atacgcgtaa aacagccagc gctggcgcga tttagccccg acgtatcccc actgttcgtc
     2041 catttccgcg cagacgatga cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg
     2101 cctgagtttt ttaaatggcg gaaaatcgtg ttgaggccaa cgcccataat gcgggcggtt
     2161 gcccggcatc caacgccatt catggccata tcaatgattt tctggtgcgt accgggttga
     2221 gaagcggtgt aagtgaactg cagttgccat gttttacggc agtgagagca gagatagcgc
     2281 tgatgtccgg cagtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga
     2341 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
     2401 taagttggca tcatcaccag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2461 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2521 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2581 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2641 gtgagttttc gttccactga gcgtcagacc ccggaaacga taggtgcaag caagttaagg
     2701 gttgcatcgc gcatgtcaat ctaggctata ccctaacttg atgtcaggca gggccgcgcc
     2761 gcttcgtcag aatagagtct gctttcccat tttttgacac atgcccgcga aggttataga
     2821 tttcagcctg acagaaatgg gctttgaggc acaacggaac agaaagtgca cttaagccgc
     2881 cttcaaccaa ggagacatcg tgcaggggca ccgcatcggc tacgtccggg tcagcagctt
     2941 tgaccagaac ccggaacgcc agctggaaca aacccaggtg agcaaggtgt tcaccgacaa
     3001 ggcatcgggc aaggacaccc agcgccccca gctcgaagcg ctgctgagct tcgtccgcga
     3061 aggcgataca gtggtggtgc acagcatgga tcggctggcc cgcaacctcg atgacctgcg
     3121 tcgcttggta cagaagctga ctcagcgcgg cgtgcgcatc gagttcctga aggagggcct
     3181 ggtgttcact ggcgaggact cgccgatggc caacctgatg ctgtcggtga tgggggcctt
     3241 cgctgagttc gagcgcgccc tgatccgcga gcggcagcgt gagggcatcg ccttggccaa
     3301 gcagcgtggc gcgtaccggg gccgcaagaa agccctgtcc gatgagcagg ctgctaccct
     3361 gcggcagcga gcgacggccg gcgagcccaa ggcgcagctt gcccgcgagt tcaacatcag
     3421 ccgggaaacc ctctaccagt acctccgcac ggacgactga cacatgccgc gtcgcttgat
     3481 cctctcggcc acggagcggg acaccctgct tgcgctgccg gaaagccagg atgacctgat
     3541 ccgctactac accttcaacg actccgacct gtcgctgatc cgccagcgac gcggcgacgc
     3601 caaccgcctc ggcttcgccg tgcagctctg cctgctgcgc taccccggtt acgcgctggg
     3661 aaccgacagc gagctgcccg agccggtcat cctgtgggtg gcgaagcaag tccaggccga
     3721 gccggcgagc tgggcaaagt acggcgagcg cgacgtgacc cgtcgcgagc atgcccagga
     3781 actgcgcacc tacctgcaac tggccccgtt cggcctgtcc gacttccgcg ccctggacct
     3841 cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg
     3901 gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg
     3961 gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg
     4021 gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc
     4081 agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc
     4141 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggacgg
     4201 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
     4261 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
     4321 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
     4381 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
     4441 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
     4501 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
     4561 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
     4621 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
     4681 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
     4741 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
     4801 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
     4861 gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
     4921 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
     4981 atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct gtgctgggcg
     5041 cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc ggcgctgctg
     5101 tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc gggcggatcg
     5161 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
     5221 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
     5281 tcgcgggacc tgtgctcggt gggctgatgg gcgatttctc cccccacgct ccgttcttcg
     5341 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
     5401 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
     5461 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
     5521 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
     5581 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
     5641 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga
     5701 ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc
     5761 cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt
     5821 ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca
     5881 gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa
     5941 cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc
     6001 tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat
     6061 aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc taggagtgcg
     6121 gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga tgatttgaag
     6181 cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc ccgggctgag
     6241 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
     6301 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
     6361 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
     6421 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
     6481 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
     6541 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
     6601 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
     6661 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
     6721 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
     6781 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
     6841 ccgcagcatc gcgaccgtca tcggtgagaa gttcggcagg tattgggtgg tgacaatgta
     6901 ggtgctgccc caaatggcag gtgctatcgc tgtgaacaat aaatcgggcg tgcgtaatga
     6961 catgcgatta caagacctcc gctacggcga tgggtttttg agagttgcag gcgccgcgat
     7021 gaccgcaccc tgacttggac tggccctgcc tcgcaccaat ccacctgcgg ctatggccgc
     7081 gatcatgcga ggcccgaaaa ttacacgacc tgtacacgtc cgccgaaccc ggaacgcaac
     7141 agagcttcgt gggcatcccg atccgggtcg tagcagcagt cctggaccaa acgcacgtcg
     7201 tagtccgcat cactagccca ggcgacgctt gaaagaacaa cgccggtggt gcttatcccg
     7261 gccatgacaa gcgtgcttac gccgcgcgtc cgaaggtcgg cgtcgagcgc cgtgccataa
     7321 aagacgctgg ctcgcggaca tgcatagaaa aggtcgcccc gttcgatcgc aagcccttcg
     7381 acgggcagac cggtgcgaaa tcgtccgctc gggagatacg gtgagatttg gcgattcgtc
     7441 gccggcggcg catgttcata ctcttcgccg agcgaaaagt tggggaacag cacgggccgg
     7501 ccgcgcaagg cgtgctggca gccgtgcaga ccctgcgtga gatgaacgcc gacaacctgc
     7561 gcaaggtgcc ggccgatgca cccacggcct tcatcaagcc gcgctggaag ccgctggtga
     7621 tcaccccgga aggcctcgac cggaaattct acgaaatctg cgccctgtcc gagctgaaga
     7681 acgccctgcg ctccggcgac atctgggtca agggctcgcg gcagttccgc gacttcgacg
     7741 actacctgct gccggccgag aagttcgccg cactcaagcg cgagcaggcc ctgcccctgg
     7801 cgatcaaccc gaacagcgac cagtacctgg aagagcgttt gcagctgctg gacgagcagt
     7861 tggccaccgt cacccgcctg gccaaggaca acgagctgcc cgatgccatc ctcaccgagt
     7921 cagggctgaa aatcaccccg ctggatgcgg cggtgccgga tcgagcgcac gctgttcatc
     7981 ctggactggc tgcaaagtgt tgaactgcgc cgccgcgtgc atgccggcct gaacaaaggt
     8041 gaggcgcgca actcgctggc cagggcggtg ttcttcaacc gccttgggga aatcagggat
     8101 cggagcttcg agcagcagcg ctaccgggcc agcggcctca acctggtgac ggcggctatc
     8161 gtgctgtgga acacggtgta cctggaacgc gccacccagg ggttggtcga ggccggcaag
     8221 ccggtggacg gcgagctgct gcaattcctg tcgccgctgg gctgggagca catcaaccta
     8281 accggcgatt acgtctggcg gcagagccgc agactggaag acgggaagtt tcggccctta
     8341 cggatgcccg gaaaacctta gcgtacgatt ttttccgaat tctgcgggct cccctatgtg
     8401 ggcgtttatg taacatatgt cgctgggcca actcttctgg tttctgggtt tttatcgtgg
     8461 attacaaggc cgtaatctga taatgcccct aatgggtcag atctctagga tagtagcaat
     8521 acagcaagga acttcttgag atcctttctg ttgcgtcgta atctcatgtc ctgcaaacaa
     8581 aaaaaccacc gctaccaacg gtggttcgtt tgccggaatc aagagctacc aactcttttt
     8641 tccgaaggta actggctttc acggagcgca gataccaaaa ctgtccttct agtgtagccg
     8701 cagtcaggcc accacttcaa gaactctgta gaagcactct actactctgc tccgtgaatc
     8761 ctgttaccag tggctgctgc cagtggcgca tttgcatgtc tttccgggtt ggactcaaga
     8821 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     8881 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     8941 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     9001 gccaccaggg ggaaacgcct ggtatcttta
//
