LOCUS       NZ_AP027594             1547 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-64-1 plasmid pLR-64-1_6, complete
            sequence.
ACCESSION   NZ_AP027594
VERSION     NZ_AP027594.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562511
            Assembly: GCF_036498065.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 294x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036498065.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:58:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,938
            CDSs (total)                      :: 4,819
            Genes (coding)                    :: 4,547
            CDSs (with protein)               :: 4,547
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 84 of 272
            Pseudo Genes (incomplete)         :: 178 of 272
            Pseudo Genes (internal stop)      :: 45 of 272
            Pseudo Genes (multiple problems)  :: 35 of 272
            Pseudo Genes (short protein)      :: 3 of 272
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027594.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-64-1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-64-1_6"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            join(1012..1547,1..>1)
                     /locus_tag="AABJ71_RS24690"
                     /pseudo=""
     CDS             join(1012..1547,1..>1)
                     /locus_tag="AABJ71_RS24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /name="NYN domain-containing protein"
ORIGIN
        1 tggcaaggtg ggccgatttc cacacggcag cggcgcccgg caaggtgggc cgattcccac
       61 gcggcagcag cgcccggcaa gatgggccga ttcccacgtg gcagcggcgc ctggcaaggt
      121 gggccgattc ccacgcggca gcggcgcccg gcaagatggg cggatttcca cgcggcagcg
      181 gcgcccggca agatgggcgg atttccacac ggcagcggcg cccggaaagg tgggccgatt
      241 cccacgcggc agcagcgccc ggcaagatgg gccgattccc acgtggcagc ggcgcccggc
      301 aagatgggcg gatttccaca cggcagcggc gcccggcaag atgggcggat ttccacacgg
      361 cagcagcgcc tggcaaggtg ggccgattcc cacgcggcag cggcgcccgg tgggtatttg
      421 cattcacttg actccccatt tgcattttac ttgactgcct gtttgcactt ctcttggtca
      481 tctccaacag aaaatagaga cagtttcctt ccgagttgac cggcagctat gagcgaaaag
      541 cggatatagg cttaatgtga gaaatgacat tttctaatac cacctcgcca ttgtgcaatg
      601 tgtaaaaaag caagaagaaa aactccgctg gcggtaaacc gcattcgggc ccccccccgt
      661 gccggagttt taccgaatgg cgcagcgggg caaccggcgc ggcctgcccc ggcgctgacc
      721 gcccgatcac tatatctggt aggcctgcca gctgagacag gcacaacatg tagtgttgcg
      781 cgccgcggcg gggcgattcg gtgttatgcg ggttttaccg aatgtctaca gatcgtgagc
      841 ggggatgaaa ggccgttatg caggattagc agggatgatt gacggggtcg tcaaaatagc
      901 atagtattgt tcctacaact caaccccgaa cgcagcgatt aagttcactg gcagttgggg
      961 tgaagaaacc ggccagcgag ccggtttttt cgtatctgat gggaagcaaa cgtgcgcacc
     1021 gcttttttca ttgatggata caatctcttt tacgggctgc tggctggaac acccttcaag
     1081 tggctcgacc tccctgcgct tctgacatat atcacgaaaa ttcaaaatcc tgcctctgag
     1141 actgcccagg taaattactt tacctctccc gttttacctc ctcttgcaac cagaggtaaa
     1201 gagtcggctg aagctcagga tgtttatatt cgcgcactca aggcgaaggg cgttacagtc
     1261 catttaggaa gacacaggct taatcgtggc agtgcgccgc gttatctgga tggtattcct
     1321 gcatcccgat ctgataaggt tgatgtatgg gatcttgagg aaaaagagac cgatgtaaat
     1381 attgctatca gtatgtaccg acttctttca aagcaacaga accttgaagc agatgcacgc
     1441 attgcacaaa tagtcttagt gtcggctgat accgatatga cgcctgcctt aaaagcaata
     1501 aaggaagatt ttccagaaat aacggttggc attattttgc cacatcg
//
