LOCUS       NZ_AP027569             2310 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-58-2 plasmid pLR-58-2_6, complete
            sequence.
ACCESSION   NZ_AP027569
VERSION     NZ_AP027569.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562499
            Assembly: GCF_036497485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 176x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036497485.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:14:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,432
            CDSs (total)                      :: 5,314
            Genes (coding)                    :: 5,113
            CDSs (with protein)               :: 5,113
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 62 of 201
            Pseudo Genes (incomplete)         :: 144 of 201
            Pseudo Genes (internal stop)      :: 40 of 201
            Pseudo Genes (multiple problems)  :: 44 of 201
            Pseudo Genes (short protein)      :: 4 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027569.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-58-2"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-58-2_6"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            452..877
                     /gene="mobA"
                     /locus_tag="AABJ83_RS27155"
                     /old_locus_tag="VEE04_51730"
     CDS             452..877
                     /gene="mobA"
                     /locus_tag="AABJ83_RS27155"
                     /old_locus_tag="VEE04_51730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001107325.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLIKDEIKIIKRIHKNKYPGSDLFK"
                     /name="mobA"
     gene            complement(911..1408)
                     /locus_tag="AABJ83_RS27160"
                     /old_locus_tag="VEE04_51740"
     CDS             complement(911..1408)
                     /locus_tag="AABJ83_RS27160"
                     /old_locus_tag="VEE04_51740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001027759.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNITQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
     Typing          1758..2016
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="100.0"
                     /evalue="6.900000000000001e-74"
ORIGIN
        1 agttatattt ttaatatata aacgcgatat caataactca cggatttata ttttatgtac
       61 atgctactat cttgctcttc cgcttcctgt tccttggaca cactccgtat cattaacagg
      121 caatggatta tataacgagg aagatctaat ctttatccct ccaataatcc acaaacgaca
      181 gacaacaaag acatcaatta attcgatgtt atttattaaa taacaagcaa taatttttaa
      241 aatctgtgta aataatattg aatgaagcat catttttttg tatcagaaat accaggttac
      301 gacgcggtat tttattttcg tcgaaaagtt gtagcggaaa attggacagg atgtgtattc
      361 gtcataccgc gaatacattc gcttgcatga gaatacctcc tgtcagggca agccccgaca
      421 ccccaaacgt gcaaaatgga gagtcattgc gatggctaaa aataaacgta gtggttcaga
      481 agtacggcag agaactaagg ttatcaccct tagagtaaat aacagagtcg caagcgaaat
      541 cagacggcga gcgaagaacg aaggactgac aatttcagaa tatatcagga cggcaagttt
      601 aaataatgaa ataaaacagc gcgtaccttc caggtattta gatgaattaa tccgacttgg
      661 tcggatgcag aagaaattat ttgataaggg aaagcggccg aaggataagg agtacctcga
      721 ggtgatgcat aaaataattt tactttgcga tgaaatgaaa atagtaacga agcgaatttc
      781 tgatgtatat aatgaaatgg atttaataaa ggatgaaata aaaataataa aaaggataca
      841 taaaaataaa tatccaggtt cggatttatt caaataaggt ctttttaaaa agaccttatt
      901 taggtgcaca ttattttatt tcttcggttg ttataatagt ttcaccgtta ggtgaatatt
      961 taattctcat ttcaaactcg cgacgctgtt caattatttt tttccattca ccacgagcaa
     1021 ataaattagg gttcgcctga tattcattac cgccacaact agcaataatc cccatttttg
     1081 ataatgaata caatctgtta cgtaatgttc cgtcttttat tcccaataat ttacagatct
     1141 ccttacggta tctagttgat aatgcaatat atccgtcata atccaatttc ctaagaatta
     1201 aaaataaaac atcttttaga gaatcaggtg cttttgtgac agcacataaa ttatcaatat
     1261 aaattttcac atacgcaggt tcttgtggaa attgaactgt tatatcggtt atttcttgtg
     1321 ttatgtttcc agtttctcta tctataacct cagtaatatt ttgtcttaaa acttttttct
     1381 tgctatccgt tctttcagta atgttcattt tatcgccttt gtcactttat aaaggtgaca
     1441 gacaatataa tgccaaaaaa acaaaatgtc actcaatata gattacgaca ataccgccca
     1501 cacgttacag gagtatcctg tacacgttac aggtgtatct tctacacgtt acaacattgg
     1561 cgatcagccc gtatgtagcc taattcggat atcttatatt tttaaatgta tccataagca
     1621 cgaggcataa tctctatatt acgaagtgac aattttaata ttgagcgtgc gtgctcaatg
     1681 tagcttccgg ttctcttccg cttcctcgcc cactcgctca ctacgttcgg tcgctggggc
     1741 tgcggcgagc ggtgccacct ctcgcatatc gttgatgatc ccgatttgat ctcaggcgat
     1801 cgagtcgctg gtcatctgtt aaacgcgctc agagcctccc agacgcacga aacggggttt
     1861 aaatgcccgt cagggcatga aaggcgcgaa aaacgcttac agagggtttc aggaggccag
     1921 gcgcgccgat acccctaacc aggggctttc aggtcgccag gaaccgcgcc agccccctgc
     1981 aaagcctgtt cgcacggcac aggtagccac tcggttggta ggtggtcgta gaccgatgat
     2041 gttgcagcgg tgggggtctg gggttctccc cagacggttt ccggttgcgc tcaaggcgct
     2101 gccggaaccg tggtggcttg gtggatttga ggcgcgttcc ggcgtttgtc ggcacgcaaa
     2161 ccatccccat ggttagttct tgccggaaac gcgccctgat gttctatcgt atcgatggtt
     2221 atatgtcgag gttgtcagca gctggcggag cactgcacgg ggattaatgc tcccattgcc
     2281 aggacaaaat aaatattaag agcaatgtta
//
