LOCUS       NZ_AP027562             2310 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-58-1 plasmid pLR-58-1_9, complete
            sequence.
ACCESSION   NZ_AP027562
VERSION     NZ_AP027562.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562503
            Assembly: GCF_036497325.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 274x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036497325.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:57:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,032
            CDSs (total)                      :: 4,911
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 70 of 230
            Pseudo Genes (incomplete)         :: 161 of 230
            Pseudo Genes (internal stop)      :: 41 of 230
            Pseudo Genes (multiple problems)  :: 41 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027562.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-58-1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-58-1_9"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            909..1334
                     /gene="mobA"
                     /locus_tag="AABJ65_RS25155"
                     /old_locus_tag="VEE07_47960"
     CDS             909..1334
                     /gene="mobA"
                     /locus_tag="AABJ65_RS25155"
                     /old_locus_tag="VEE07_47960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001107325.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLIKDEIKIIKRIHKNKYPGSDLFK"
                     /name="mobA"
     gene            complement(1368..1865)
                     /locus_tag="AABJ65_RS25160"
                     /old_locus_tag="VEE07_47970"
     CDS             complement(1368..1865)
                     /locus_tag="AABJ65_RS25160"
                     /old_locus_tag="VEE07_47970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001027759.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNITQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 ggggtttaaa tgcccgtcag ggcatgaaag gcgcgaaaaa cgcttacaga gggtttcagg
       61 aggccaggcg cgccgatacc cctaaccagg ggctttcagg tcgccaggaa ccgcgccagc
      121 cccctgcaaa gcctgttcgc acggcacagg tagccactcg gttggtaggt ggtcgtagac
      181 cgatgatgtt gcagcggtgg gggtctgggg ttctccccag acggtttccg gttgcgctca
      241 aggcgctgcc ggaaccgtgg tggcttggtg gatttgaggc gcgttccggc gtttgtcggc
      301 acgcaaacca tccccatggt tagttcttgc cggaaacgcg ccctgatgtt ctatcgtatc
      361 gatggttata tgtcgaggtt gtcagcagct ggcggagcac tgcacgggga ttaatgctcc
      421 cattgccagg acaaaataaa tattaagagc aatgttaagt tatattttta atatataaac
      481 gcgatatcaa taactcacgg atttatattt tatgtacatg ctactatctt gctcttccgc
      541 ttcctgttcc ttggacacac tccgtatcat taacaggcaa tggattatat aacgaggaag
      601 atctaatctt tatccctcca ataatccaca aacgacagac aacaaagaca tcaattaatt
      661 cgatgttatt tattaaataa caagcaataa tttttaaaat ctgtgtaaat aatattgaat
      721 gaagcatcat ttttttgtat cagaaatacc aggttacgac gcggtatttt attttcgtcg
      781 aaaagttgta gcggaaaatt ggacaggatg tgtattcgtc ataccgcgaa tacattcgct
      841 tgcatgagaa tacctcctgt cagggcaagc cccgacaccc caaacgtgca aaatggagag
      901 tcattgcgat ggctaaaaat aaacgtagtg gttcagaagt acggcagaga actaaggtta
      961 tcacccttag agtaaataac agagtcgcaa gcgaaatcag acggcgagcg aagaacgaag
     1021 gactgacaat ttcagaatat atcaggacgg caagtttaaa taatgaaata aaacagcgcg
     1081 taccttccag gtatttagat gaattaatcc gacttggtcg gatgcagaag aaattatttg
     1141 ataagggaaa gcggccgaag gataaggagt acctcgaggt gatgcataaa ataattttac
     1201 tttgcgatga aatgaaaata gtaacgaagc gaatttctga tgtatataat gaaatggatt
     1261 taataaagga tgaaataaaa ataataaaaa ggatacataa aaataaatat ccaggttcgg
     1321 atttattcaa ataaggtctt tttaaaaaga ccttatttag gtgcacatta ttttatttct
     1381 tcggttgtta taatagtttc accgttaggt gaatatttaa ttctcatttc aaactcgcga
     1441 cgctgttcaa ttattttttt ccattcacca cgagcaaata aattagggtt cgcctgatat
     1501 tcattaccgc cacaactagc aataatcccc atttttgata atgaatacaa tctgttacgt
     1561 aatgttccgt cttttattcc caataattta cagatctcct tacggtatct agttgataat
     1621 gcaatatatc cgtcataatc caatttccta agaattaaaa ataaaacatc ttttagagaa
     1681 tcaggtgctt ttgtgacagc acataaatta tcaatataaa ttttcacata cgcaggttct
     1741 tgtggaaatt gaactgttat atcggttatt tcttgtgtta tgtttccagt ttctctatct
     1801 ataacctcag taatattttg tcttaaaact tttttcttgc tatccgttct ttcagtaatg
     1861 ttcattttat cgcctttgtc actttataaa ggtgacagac aatataatgc caaaaaaaca
     1921 aaatgtcact caatatagat tacgacaata ccgcccacac gttacaggag tatcctgtac
     1981 acgttacagg tgtatcttct acacgttaca acattggcga tcagcccgta tgtagcctaa
     2041 ttcggatatc ttatattttt aaatgtatcc ataagcacga ggcataatct ctatattacg
     2101 aagtgacaat tttaatattg agcgtgcgtg ctcaatgtag cttccggttc tcttccgctt
     2161 cctcgcccac tcgctcacta cgttcggtcg ctggggctgc ggcgagcggt gccacctctc
     2221 gcatatcgtt gatgatcccg atttgatctc aggcgatcga gtcgctggtc atctgttaaa
     2281 cgcgctcaga gcctcccaga cgcacgaaac
//
