LOCUS       NZ_AP027527              588 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-33 plasmid pLR-33_4, complete sequence.
ACCESSION   NZ_AP027527
VERSION     NZ_AP027527.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562513
            Assembly: GCF_036496145.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 588)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 152x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036496145.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:48:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,800
            CDSs (total)                      :: 4,682
            Genes (coding)                    :: 4,488
            CDSs (with protein)               :: 4,488
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 60 of 194
            Pseudo Genes (incomplete)         :: 126 of 194
            Pseudo Genes (internal stop)      :: 42 of 194
            Pseudo Genes (multiple problems)  :: 34 of 194
            Pseudo Genes (short protein)      :: 4 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027527.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-33"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-33_4"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
ORIGIN
        1 atagtgctat atattaatat atatcgctat atagttagag ccagatccag agcagatccc
       61 aaacgaccac acccaaaaag acagaaccgc agtcagtgcg gtcagtgaaa tattataaag
      121 tattgatata taaggctttt ttacaccgac cacacaaacc acaccaacca cacaaaaaaa
      181 tacaccaccc aatatattta tagatccctt attcttaatc cttattattt cttattctga
      241 aatccttatt ccgaaaccca cagggttaag gattaacctt attccctatc ccgtacggga
      301 tagggaataa ggttaatcct taaccctgtg ggtttcggaa taaggatttc agaataagaa
      361 ataataagga ttaagaataa gggatctata aatatattgg gtggtgtatt tttttgtgtg
      421 gttggtgtgg tttgtgtggt cggtgtaaaa aagccttata tatcaatact ttataatatt
      481 tcactgaccg cactgactgc ggttctgtct ttttgggtgt ggtcgtttgg gatctgctct
      541 ggatctggct ctaactatat agcgatatat attaatatat agcactat
//
