LOCUS       NZ_AP027525            15486 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-33 plasmid pLR-33_2, complete sequence.
ACCESSION   NZ_AP027525
VERSION     NZ_AP027525.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562513
            Assembly: GCF_036496145.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15486)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 152x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036496145.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:48:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,800
            CDSs (total)                      :: 4,682
            Genes (coding)                    :: 4,488
            CDSs (with protein)               :: 4,488
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 60 of 194
            Pseudo Genes (incomplete)         :: 126 of 194
            Pseudo Genes (internal stop)      :: 42 of 194
            Pseudo Genes (multiple problems)  :: 34 of 194
            Pseudo Genes (short protein)      :: 4 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027525.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-33"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-33_2"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            complement(115..324)
                     /locus_tag="AABJ50_RS23890"
                     /old_locus_tag="VEE12_45720"
     CDS             complement(115..324)
                     /locus_tag="AABJ50_RS23890"
                     /old_locus_tag="VEE12_45720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057483821.1"
                     /translation="MTIELQLYSMLVIASLFFTFKAIVSRKKTWAFVSANNAEMTKKYN
                     NKFFTYTVIGIFSWFITVLATSSM"
                     /name="hypothetical protein"
     gene            complement(396..2519)
                     /locus_tag="AABJ50_RS23895"
                     /old_locus_tag="VEE12_45730"
     CDS             complement(396..2519)
                     /locus_tag="AABJ50_RS23895"
                     /old_locus_tag="VEE12_45730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338380942.1"
                     /translation="MTDKNENIEIHNQLWAELKSGLQIKADGFWTPEKDGNRSCFHPFG
                     ICPIAVTKAIGENGKTAFTSIRVKYRAISTDLEGNPEYEEVEIPLEDFNSLSHKYLKNY
                     MPPAKQNTKTTIAQCLQEVLANPREHDIKTKQAYTYCGWVQLKDKQGNLRWKHIRPGSD
                     MFATTAEEDNVADNSGTAGSEAVYKEIQLKAMRASLLYAYLTAHYFGAYAKGIIPRSAD
                     FSPIIYIKGQRSRGKTTFLFLLGSHQGRPDKRFTVFDSKSTVQGLQRLMPWFKHGGFCL
                     DEIDKFMNDRDGMENLMAYANNGGRVVAHKDKGVKFQDTWDIVIFSTGNTDMTQHKNFK
                     EDGKAGAVLSRFDIRDIGSKELTGFKVETEEDKKDLASFVSTLRNNYGWGYQGVVKLLE
                     EAEYEAEVKRNSGDSSAISRYHEVYNSFFDEISEDKELHSIDEEKRLSEHLAFAKVGAE
                     LMKDYFGEEAWEIANKAIQIVLDEYRTAVTEHGDIVFTREEREALERYEEIFDFVSNNT
                     GNFVWENYAYDNEISQKQKAEILSREAREGNNKVYGTVIQNKHMNSPRDLLGEMYLSSD
                     GEKAYGDNKIQQLRQDLSTLGLLVQKTKRSGEVEDRVDISKNARSAWGFSRALRFKLIH
                     PDEFEKQRRDELRRQAEARSCEEELKRKTALDQIEDIFSFNNLSEEEINLNKKYNSDPA
                     FDDYAPYDNDYDN"
                     /name="hypothetical protein"
     gene            complement(2580..3269)
                     /locus_tag="AABJ50_RS23900"
                     /old_locus_tag="VEE12_45740"
     CDS             complement(2580..3269)
                     /locus_tag="AABJ50_RS23900"
                     /old_locus_tag="VEE12_45740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065809931.1"
                     /translation="MDLEKLQALKAKLNNNDTEAGGELPALQDTNTVIEDTNDELVYYP
                     AATVEEAREFMAGSFKFLDKYPLNGQISRIQRVKDKENYKEPKIKTFGTLNIEFDKNEL
                     LISCSQDFKIKYMQDFQHYFYTNLIEKWYNDCGIYIKGTDNFIVYSLICAILSQRKIFP
                     LFCDRPLLEKALEKITADFNSKQSYISEIFDIQDRNVILRRKKIYMLAFESAVKNNKTL
                     NLKKEFV"
                     /name="hypothetical protein"
     gene            complement(3284..5245)
                     /locus_tag="AABJ50_RS23905"
                     /old_locus_tag="VEE12_45750"
     CDS             complement(3284..5245)
                     /locus_tag="AABJ50_RS23905"
                     /old_locus_tag="VEE12_45750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338380943.1"
                     /translation="MANVTILSNLLTSDFRKKISEGGSPAVNGDPSIGDLASYMDPFTG
                     NKITALNAVELVQKLDEQGIDLVERDKILELQKQEDNNFSFEKEIERKEFNARINTPIF
                     EKFINTLIDKGLVEFNDPNFFKSFIKQSWEEKLDKDFFIKAEEKGLMSFPGELDNEYKL
                     EGVNKFFAVYSPKFDELKQTEYIKAIRPRATANIKYEYTQIGWDLSQYGSEERQLLANN
                     IIIGTLEHPDMTFQGDRVVGYRSVLAYPRHIDQERGAHGQAWIHPTTLYHYDDNGIKRT
                     LVQPLTDINDSKGQAAILEAINKNLKAAGLPELATIQSNRINKGTDPEAHNQARQAAFT
                     GNLPELPVIEDNDPGMNTAQAGDYYRRMEEHQTRELADLFARQKALVDQINAAKAARIA
                     VETNAILENENNKLSKELDNKNNIIEELKSSHKEEITSLNESHNNEIQAYIKKNSELAS
                     ELAEQTEIAQKVPELEKEVSGLEEDNTALTKSNLYKARGFDRLLKIAKAIRDTLKNEVK
                     SLKDKLFKVEEENGVLKETNKELVSGNATLKADNDKLQSENSDLKNKVKNQTKIEIEME
                     ALKEENKLLKETAKEVAKLKIQNTKLSMDYDTQREIIKMYEDTLVEKKIIPNENRVIPK
                     EEPKAENENDNKNKGPKL"
                     /name="hypothetical protein"
     gene            complement(5353..5673)
                     /locus_tag="AABJ50_RS23910"
                     /old_locus_tag="VEE12_45760"
     CDS             complement(5353..5673)
                     /locus_tag="AABJ50_RS23910"
                     /old_locus_tag="VEE12_45760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057483825.1"
                     /translation="MTDKKTDLLEQINRLTEISDEVIFNESNTYFDFTLKNNDSNSVCV
                     ITDASIKEHSRKLLVRITKDLDEALDKHTMGTKGVSIVALAEWALKELNKRNETLLIKN
                     KE"
                     /name="hypothetical protein"
     gene            complement(5756..6460)
                     /locus_tag="AABJ50_RS23915"
                     /old_locus_tag="VEE12_45770"
     CDS             complement(5756..6460)
                     /locus_tag="AABJ50_RS23915"
                     /old_locus_tag="VEE12_45770"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_101989970.1"
                     /translation="MNIEKELELRKNIIKISVNSPKGGVGKSTSSLILAYFFAKIANKN
                     VVIVERDPQGTCSLFYQGALKKDKAVFKVVKELSKEDIDTANVIIWDHRPEWDMDVLGD
                     IVVVPYQANGESIFPTVKAINEFNKMGKKVLAFANKTKPRLSALEKNVINTFFPERENH
                     KTIIVPERQAYPNAYIKSTTVFDEDCGIPFADKAKDEFYEVIQGILDLYTGKRESDKIE
                     PSKIAEINSSED"
                     /name="ParA family protein"
     gene            6616..8898
                     /locus_tag="AABJ50_RS23920"
                     /old_locus_tag="VEE12_45780"
     CDS             6616..8898
                     /locus_tag="AABJ50_RS23920"
                     /old_locus_tag="VEE12_45780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="WP_338380944.1"
                     /translation="MRTKNKNFIYLLFMLLFVVAHSQTAQAFDLFELPEEDLMNKYFFD
                     PLFGDNSVIGPLLAVLNGGVLTLGGVLFIYNVLIMTERTAAKADKLVNSRWYFVRLPIA
                     VALMMPINNGFSPIQTIVVWSAKQGVGLATQVYNVLAITSFEGALYIPPKLTSKVEKVA
                     YGMFINNVCVNAFNKAINDYEPETKNLFFRNSFKMGLLAPNPVNNKYFSSIDYSFKNKE
                     IQPDYATNTFYQPYFCGRAVQNLNNNTSAKNIMKTEAGKMLNVDAALTKFSPVQASEFI
                     NLQNSMFVLSQSFLSGSETESSVYNKIQVAANSYQKNISIAAQAAFLTSVNPDYIKNMM
                     KDGILMFGAYPNQISAAADQVTTAMSNLPNIDLPKLSGASEFSTQTALYDAFLNVQKAK
                     DFAKNGALSSNSGIHNLGNAETNSTITGSLLSWFTQDNNLGIGNDEGAIASKNLLLAAK
                     SEGNKLINAAWASFAAATGFAVAGGAASSNLISSVVGADGGFQSLMQMLTPVFMTIFFS
                     LLGAGITLAVFIPLLVFITWSMAIIHYVLEVCLAVCAAPFWALSHTVDKGEGLIGTASK
                     GYTELLSLVSRPALMVLGGAFSMKLLSAVNGFINEGFAAVFNLNVISDTSGLGITIFCG
                     SIVVYSLCMLALTKKIISCITLFPDKFMTWIGGATGDMSSPVKDIGATTAIAAGSTIGA
                     MKVGNGSSLANVAKSMAGGNKAKPKSPVNTPNFDNANNTSSGSAKENNAEPNKQAFKHL
                     KDLNRDN"
                     /name="DotA/TraY family protein"
     gene            8997..9644
                     /locus_tag="AABJ50_RS23925"
                     /old_locus_tag="VEE12_45790"
     CDS             8997..9644
                     /locus_tag="AABJ50_RS23925"
                     /old_locus_tag="VEE12_45790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_219833742.1"
                     /translation="MESKIFKNLQIAVFREQVMNIKNHNNIIDIVETMKDKVSFDILIY
                     HSVIAYMSKVELLALQYSHYIETKKSITDSIKNVRDFICAYEKKRQKKVKDMDYNELKE
                     RQKYKEFKRNISLIDNMSDLYSGNDKEFYHEIAEQLKRYDDLIYHLLEHKDCNLTDDNF
                     ISLIEDMKKFKGKRKMNNEIFESLHTKYITILSDRVLHLVFENAEPKLKGRV"
                     /name="hypothetical protein"
     gene            9646..9939
                     /locus_tag="AABJ50_RS23930"
                     /old_locus_tag="VEE12_45800"
     CDS             9646..9939
                     /locus_tag="AABJ50_RS23930"
                     /old_locus_tag="VEE12_45800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_219833743.1"
                     /translation="MDIILTIFVYIPAVLFYILLLSELYKLILKNIKRKIKRKVKTEIL
                     DSFRNWKGSLNEFEEELETSVTDYFDSENDGLTTAEITASDLVAYEDNRKRK"
                     /name="hypothetical protein"
     gene            10083..10556
                     /locus_tag="AABJ50_RS23935"
                     /old_locus_tag="VEE12_45810"
     CDS             10083..10556
                     /locus_tag="AABJ50_RS23935"
                     /old_locus_tag="VEE12_45810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338380935.1"
                     /translation="MKLFKKTIQKNKDRITNNGSFQILKYFFYPQKREERKYSGFAEAL
                     ADYNTDEATLQKSYRNFCFMAYMWGFAFLANLFYLLMGLFIDGQFHASNFFFGCICFGC
                     WFKGAAYAYSLKYHTLEVIRVLMSPLDWFPDFTHKVKMVYKDKPASRNIDIRR"
                     /name="hypothetical protein"
     gene            10759..11334
                     /locus_tag="AABJ50_RS23940"
                     /old_locus_tag="VEE12_45830"
     CDS             10759..11334
                     /locus_tag="AABJ50_RS23940"
                     /old_locus_tag="VEE12_45830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338380936.1"
                     /translation="MENEIINIHFKLPGQFITDNDLATMFKRKIVVIMDRRGTGKTTIL
                     KNISNKLTLPSKSVFIDDLSFNGNVNKRLLRNNIQNYIKKGVFVFISTSCNYSVVNYYL
                     KPHLDYIMYDSVFLFDALNGGLGDSTSLYVGSREECYERFLKGEITNENVDTEKTDMNI
                     INEFIAERDKTQITEILKSKQVYNKRRL"
                     /name="hypothetical protein"
     gene            11348..12055
                     /locus_tag="AABJ50_RS23945"
                     /old_locus_tag="VEE12_45840"
     CDS             11348..12055
                     /locus_tag="AABJ50_RS23945"
                     /old_locus_tag="VEE12_45840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058683441.1"
                     /translation="MKRAIINLNNKILLSDIFKKEIDNKIEKNYLTLHNYITNGDLFKR
                     EDFNSLLERRMLNLGLYDEKQFRQSFKDSLLINNLKNFVGDMFSHSKYSNLEHSLQDTI
                     TNRQVLVSEEDFKLTNDKIVNSIRNNNLNGFHCILHINTMPTAVYYVSHKNDIYENIEK
                     VQREVIDSEKEKIEKARLDLLDIKSKYYDKFNKGELDILINPVLLSEFLVKKENEVLTD
                     ILDSKQTSSKRRL"
                     /name="hypothetical protein"
     gene            12066..12272
                     /locus_tag="AABJ50_RS23950"
                     /old_locus_tag="VEE12_45850"
     CDS             12066..12272
                     /locus_tag="AABJ50_RS23950"
                     /old_locus_tag="VEE12_45850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338380937.1"
                     /translation="MKNNVRYNCKAILEELKSDGVLDSFVIWSSSIYPDIHLSQSCFES
                     CYEIYKLNENFFKENWNKKIISS"
                     /name="hypothetical protein"
     gene            12362..12691
                     /locus_tag="AABJ50_RS23955"
                     /old_locus_tag="VEE12_45860"
     CDS             12362..12691
                     /locus_tag="AABJ50_RS23955"
                     /old_locus_tag="VEE12_45860"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_338380938.1"
                     /translation="MEYLEFVETSVFTRECKTLLTDDEYKEFQTHLLLDPEAGSIIVGT
                     GGCRKVRWARQGTGKSSGIRAIYYYYNPAGRLYMLLVYPKSEKDSLSAAEKNQLKAVVS
                     VFKES"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            12695..12985
                     /gene="nadS"
                     /locus_tag="AABJ50_RS23960"
                     /old_locus_tag="VEE12_45870"
     CDS             12695..12985
                     /gene="nadS"
                     /locus_tag="AABJ50_RS23960"
                     /old_locus_tag="VEE12_45870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_338380939.1"
                     /translation="MRKELFEELIASAKEAVEIHQGIREPARITRMAIPDVKAIRSNTG
                     LKQDDFASLVGVSPALVQAWEQHRRTPSGSSLKLLRMIELKPDLIDTLKLA"
                     /name="nadS"
     gene            13274..13762
                     /locus_tag="AABJ50_RS23965"
                     /old_locus_tag="VEE12_45880"
     CDS             13274..13762
                     /locus_tag="AABJ50_RS23965"
                     /old_locus_tag="VEE12_45880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338380940.1"
                     /translation="MKKQNTQYSTFTSYLSKTNKNVDMYRLYNPMFDNLNYFGFAYDIN
                     FYGSYFKRHNITKRNFLTIFSFYCYKLSNERFLGNKEIYMEDKSLFIKASMKLFKYKIK
                     HLSDNIDNLNISFRGGNVIFISQFGKKEEISYFTLSKIAGDIFDIKDFSMSNEKLLAA"
                     /name="hypothetical protein"
     gene            13783..14571
                     /locus_tag="AABJ50_RS23970"
                     /old_locus_tag="VEE12_45890"
     CDS             13783..14571
                     /locus_tag="AABJ50_RS23970"
                     /old_locus_tag="VEE12_45890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187623.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_163521007.1"
                     /translation="MKKQFKKTLESIQTRSNRSISNTVREFFNCVSYTMIINSFFQLEQ
                     RELFYLGDNIHSINKLLTQSGLINKSITLTDRHEFKIEEKFYNQFEREIIEIANLLSIE
                     INKNPLTDIFNELFEECYLSGKKGGGFAQYLTPNRVSIGAVKFLALTKKEKYHIGDICG
                     GTGSMILALASEIYKKEGLEGIQKIDLFYQDKDPFISQIFIAQILTNMIYHNFDFNELR
                     VYIGDSITEYDTINTLFLRVKQNKLVAQRVLEIDKEKKAA"
                     /name="N-6 DNA methylase"
     gene            14592..15380
                     /locus_tag="AABJ50_RS23975"
                     /old_locus_tag="VEE12_45900"
     CDS             14592..15380
                     /locus_tag="AABJ50_RS23975"
                     /old_locus_tag="VEE12_45900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187626.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_338380941.1"
                     /translation="MKKQFYKAMENILTRSNRSHSDTVREFFNCVSYTMIMNSLYQLEK
                     REQFYSGETTYRFSTILNELHVAERNIIITNRHKLKIDNVFYKRFANEIKFISGVLSNE
                     IGKNPLTDIFNELFEDLFLTDKKGDGFGQFLTPHDISELLTDIIYTTSKDKYKIADSCA
                     GTGSLIFPLLKRIFLKEGFEGIQKVELFYNDKDSFVSQLFIAQILTNMIYHNLDFKDLR
                     VYIGDAITEYDTIKTLFLRVKQNKLVAQRVLEIDREKKAA"
                     /name="N-6 DNA methylase"
ORIGIN
        1 gtaaggtgcg ggtataaaaa aagcccactc taaaattgaa tgggcttgaa tataaaaact
       61 aaatataaaa aggagatcgt aaactactaa acagaagtta aattgtcaat caaattacat
      121 tgaagaagtt gctaatactg taataaacca agagaaaata ccaattactg tataagtgaa
      181 aaatttatta ttatactttt tggtcatttc tgcattattg gcactaacaa aagcccaggt
      241 ttttttacgg gatacaattg ctttaaaagt aaaaaataaa gaagcaatta cgagcattga
      301 ataaagttgt aattcgatag tcattttaaa atctcctgtt tttgtgttta cataattaat
      361 ttagcgtact cttttagctt tagcaaattt attttttaat tatcgtaatc attatcatac
      421 ggtgcataat catcaaaagc tggatcactg ttatattttt tatttaaatt aatttcttct
      481 tcactcaaat tattgaagct aaaaatatct tcaatctgat caagagccgt ttttctcttt
      541 aattcttctt cacacgaacg agcctcagct tgtcttctaa gctcgtccct tctttgtttt
      601 tcgaattcat ctgggtgaat aagtttaaat cttaatgctc ttgaaaaacc ccaagcacta
      661 cgggcatttt tactaatatc aacacgatct tcaacttcac cacttctttt agttttttgc
      721 actaaaagcc ccaatgttga taaatcttgt cttaactgct gtattttatt atcaccataa
      781 gctttttcac catcactact taaatacatt tcacctaata aatcgcgtgg tgaattcata
      841 tgtttatttt gaatgacagt tccataaact ttattgttcc cttcccttgc ttcacgagaa
      901 agaatttcag ctttctgttt ttggctaatt tcattatcgt aagcataatt ttcccataca
      961 aaattaccag tattattgct tacgaaatca aaaatttctt catatctttc taaggcttct
     1021 ctttcttctc ttgtaaatac aatgtcgccg tgttcagtta ctgctgttct gtattcgtct
     1081 aatactattt gaattgcttt gttagcaatt tcccaggctt cttcaccaaa ataatctttc
     1141 atcaattcag cacctacttt tgcaaatgct aaatgttcac tcaatctttt ttcttcgtcg
     1201 attgaatgca attctttatc ttctgaaatt tcgtcaaaga aactattata tacttcgtga
     1261 taacgagata tagccgaact atccccacta tttcttttta cttctgcttc atattccgct
     1321 tcttctaata atttaacgac accttggtaa ccccagccgt aattatttcg taaagtagat
     1381 acaaaggaag ctaaatcttt tttatcttct tctgtttcaa ctttaaagcc tgttaattct
     1441 tttgagccaa tgtctctaat atcaaaacga gataatacag ccccagcttt accgtcctct
     1501 ttgaagttct tatgctgggt catatcggtg ttaccagtgg agaagataac aatgtcccag
     1561 gtatcctgga atttaacccc tttatctttg tgagcaacga cacggccacc gttgttagca
     1621 tacgccatca ggttctccat accgtccctg tcgttcataa atttgtctat ctcatcgaga
     1681 cagaaaccac cgtgtttaaa ccacggcata agcctctgta agccctgtac ggtagactta
     1741 ctatcaaaga cagtaaaacg cttgtctggt cgtccctggt ggcttcctaa caggaacaga
     1801 aatgtagtct tacctctgga tctctgtccc tttatgtaaa tgattggtga gaagtcagca
     1861 gaacgtggaa tgataccctt ggcatatgca ccgaagtagt gagctgtaag gtaagcatag
     1921 agaagactgg ctctcatagc ctttaattgt atttctttat agacagcttc gcttccagcc
     1981 gttcctgagt tatcagcaac attatcttct tctgcggtag tcgcaaacat atcactaccg
     2041 ggacgaatat gcttccatct taaattacct tgtttatctt ttaattgaac ccaaccacag
     2101 tatgtataag cctgttttgt ttttatatcg tgttctcttg ggtttgctaa aacttcttgc
     2161 aaacattgtg caatagtcgt tttagtattt tgttttgccg gtggcatata gttttttaag
     2221 tatttatggc ttaaagagtt gaaatcttct aacggaattt caacttcttc atactctgga
     2281 ttaccttcca gatcggtaga aatcgctcta tattttacac ggatagaagt aaaagcagtt
     2341 ttaccatttt caccaatggc ttttgtaact gcaatcgggc aaataccaaa tggatggaaa
     2401 cagcttctat taccgtcttt ctctggtgtc cagaagccgt ccgctttaat ttgaagacct
     2461 gattttaatt cagcccatag ctgattatgt atttctatat tttcgttttt gtcagtcata
     2521 ctattttcct ttttacattc atttttattg tatttattta tattcaaaat ttactactat
     2581 tatacaaact ctttttttag atttaaagtc ttattgtttt ttactgctga ctcgaaagca
     2641 agcatatata ttttttttct tcttaatata acattacgat cttgtatgtc aaaaatttca
     2701 gatatataac tttgcttaga attaaaatca gcagtgattt tttctaatgc tttttctaat
     2761 aatggtctat cacaaaacaa aggaaatatt tttctttgtg ataaaattgc acaaattaag
     2821 gaatatacta taaagttgtc agtacctttt atgtatattc cgcaatcgtt ataccatttt
     2881 tcaattaaat tagtataaaa ataatgctgg aaatcctgca tatattttat tttaaaatct
     2941 tgtgagcaag aaattaaaag ttcgttttta tcaaattcaa tatttaaagt accaaaggtt
     3001 tttatttttg gttctttgta gttttcttta tcttttactc gttgaatacg gctaatttgc
     3061 ccattcaatg ggtatttatc cagaaattta aaactacccg ccataaattc acgggcttct
     3121 tctactgttg ccgctgggta ataaactaat tcgtcgttag tatcttctat tacagtattt
     3181 gtatcttgta atgctgggag ttccccccca gcctccgtat cattattatt aagtttggct
     3241 tttaaagcct gtaatttttc taaatccata attttctcct ttattataat ttaggacctt
     3301 tatttttatt atcattttca ttttctgctt ttggttcttc ttttggaatt acacgatttt
     3361 catttggaat aatttttttc tcaacaagtg tatcttcata cattttaata atttctcgtt
     3421 gagtatcata atccatagat aacttagtat tttggatttt aagttttgct acttctttcg
     3481 ctgtctcttt gagaagtttg ttttcttctt taagagcttc catttcaatt tcaattttgg
     3541 tctgattttt taccttgttt ttaagatctg aattctcact ttgtaattta tcattatcag
     3601 cttttaaagt agcattacca gaaaccaatt ctttattagt ctcctttaat actccatttt
     3661 cttcttctac tttgaataac ttatctttga gtgatttaac ttcatttttc aaagtatcac
     3721 gtattgcttt tgcaatttta agaagtctat caaagcccct tgctttatat aaattacttt
     3781 tagttaaagc tgtattatct tcttcaagtc cagaaacttc tttttctaat tcaggtactt
     3841 tttgagctat ttcggtttgt tctgcaagtt cacttgcaag ttcactattc tttttaatgt
     3901 aagcctgaat ttcattatta tgactttcat ttaaagaagt aatttcttct ttatgtgaag
     3961 attttaattc ttcaataata ttgtttttat tgtctaattc ttttgaaagt ttattatttt
     4021 cattttccaa aatagcattt gtttcaacag caatacgagc agcttttgct gcattaattt
     4081 gatctactaa tgctttttgc cttgcaaaca aatcagccaa ttctctggtc tggtgttctt
     4141 ccattcgtct gtaatagtcg ccagcctgag cagtattcat accaggatca ttatcttcaa
     4201 taactggtag ttctggaagg ttaccagtaa atgccgcttg tcttgcttga ttgtgagctt
     4261 ctggatcagt tcctttattg attctgttag attgaatggt ggcaagttct ggcaagccag
     4321 cagcttttaa gtttttatta atagcttcga gtatagcagc ttgaccttta ctatcattaa
     4381 tatcagttaa aggctgaacc aaagttctct ttataccatt atcatcatag tgatataaag
     4441 ttgttgggtg aatccaggct tgaccatgtg cccctctttc ctggtctata tgacggggat
     4501 aagctaaaac agacctataa ccaacaacac gatcaccttg aaaagtcata tcagggtgtt
     4561 ctaatgttcc aattattata ttatttgcta aaagttgtct ttcttctgag ccatactgtg
     4621 ataaatccca gccaatctga gtgtattcat atttaatatt agcagtagca cgcggacgaa
     4681 ttgctttaat atattcagtt tgttttaatt cgtcgaattt cggtgagtaa acagcaaaaa
     4741 acttatttac accttctaat ttatattcat tatctaattc accagggaat gacattaacc
     4801 ctttttcttc tgccttaatg aaaaaatctt tgtctaattt ttcttcccag gattgcttaa
     4861 taaaagattt aaagaaatta ggatcattaa attctactaa acctttatca ataagagtat
     4921 taataaattt ttcaaaaatt ggagtattta ttcttgcatt aaattctttt ctttcaattt
     4981 ctttttcaaa agaaaagtta ttatcttctt gtttttgcag ttcaaggatt ttatctcttt
     5041 caactaaatc tatcccttgc tcatctaatt tctgaaccag ttcaacggca tttagtgcag
     5101 taattttatt acctgtgaaa gggtccatat aagaagcaag atcaccaatg ctgggatcac
     5161 cattaacagc aggtgaacca ccttctgata ttttttttct aaagtcgctt gttagtaaat
     5221 tacttaatat tgttacgttc gccataatta tttttccttt ttaagttagt ttattatcaa
     5281 ataaaaaata accttgttct ttatttctta ttctatatat atcttaacgc aagaataaga
     5341 attaacaata gcttattcct tattttttat aagtaatgtt tcatttcttt tatttaattc
     5401 ttttaatgcc cattcagcca aagcgacaat agaaacccct ttggttccca tagtgtgttt
     5461 atctaaggct tcgtctaaat ctttggtaat gcgaactaag agcttgcggc tatgctcttt
     5521 aatgctggca tcggtgatta cacaaacaga attactgtca ttatttttaa gagtaaaatc
     5581 aaagtatgta ttactctcat tgaaaataac ttcgtccgag atttctgtca gtctgtttat
     5641 ttgttctaac aaatctgttt ttttgtctgt cattttaaat ctcctgtctt ttatctttgt
     5701 tatatagcgg tatatatcgc tatatattac tatatattcg atattttaaa taaagtcaat
     5761 cttctgaact atttatttca gcaatttttg aaggctctat tttatcgctt tctcttttac
     5821 cagtataaag atctaaaatg ccttggataa cttcatagaa ttcatctttt gctttatcag
     5881 cgaatggaat tccgcaatct tcgtcaaata cggtagttga ttttatatag gcatttggat
     5941 aagcctgtct ttcgggaacg ataatcgttt tatgattttc tcgttctgga aagaaagtat
     6001 taattacatt cttttcaagt gctgaaagac ggggtttagt tttatttgca aaagctaaaa
     6061 cttttttacc cattttgtta aattcattaa tagccttaac agtcgggaaa atgctttcgc
     6121 catttgcctg ataaggaact actacaatat cccccaaaac gtccatgtcc cactctggac
     6181 ggtgatccca tatgattaca ttagctgtat ctatatcttc ttttgatagc tctttaacga
     6241 ctttaaagac ggctttatct ttcttcaaag caccctgata gaacagtgaa caagtaccct
     6301 gtggatctct ttcaacaatg actacatttt tattagcaat cttagcgaag aaataagcca
     6361 aaatcagcga actggtgctt ttcccaacac ctcctttagg tgaattgacg gaaatcttaa
     6421 taatattttt tcttaattct aattcttttt ctatattcat tttcttattt cctattgaca
     6481 aattaattat tagatatata acagtatata ttgctatata tcactatata ctgaatatta
     6541 gtccttaatt aactaaaagt caagaaaaag gagagaaata gcaatgaaat tataaataaa
     6601 aaaggagtaa taaatatgag aactaaaaat aaaaacttta tatatctttt gtttatgttg
     6661 ttatttgtag tagcacattc acaaacagca caggcatttg atttattcga actgccagaa
     6721 gaagacctaa tgaataaata cttttttgat cctttattcg gtgataatag tgttattggt
     6781 ccattattag cagtcctaaa tggcggtgtt ttaacgttgg gtggtgtttt gttcatatat
     6841 aatgtgttaa ttatgacaga acgaacagcg gcaaaagcgg ataaattagt aaatagccgt
     6901 tggtattttg tcagattacc gatcgctgtc gctttaatga tgcccattaa taacggtttt
     6961 tcacctattc aaacaatagt tgtatggtca gcaaagcaag gggtagggct tgcaactcaa
     7021 gtttataatg ttttagctat aacttctttt gaaggtgctt tatatatacc tccaaaacta
     7081 acttcaaagg ttgaaaaagt agcttatgga atgtttatta ataatgtttg cgtaaatgct
     7141 tttaataaag ccataaatga ttatgaaccc gaaactaaaa atttattttt cagaaactct
     7201 tttaaaatgg gtttattagc tccaaatcct gtaaacaata aatattttag ttctatcgat
     7261 tactcattta aaaataaaga aatacaacct gattatgcaa ccaatacttt ttatcaacct
     7321 tacttctgtg gtagagcagt tcaaaacctt aataataata cttctgcaaa aaatataatg
     7381 aaaacagaag ctggtaaaat gttaaatgta gatgctgctt taacaaaatt cagcccagtt
     7441 caggcaagtg aatttattaa tctacaaaat tctatgttcg ttctgtcaca aagtttttta
     7501 agtggaagtg aaacagaaag tagtgtttat aataaaatac aagttgcagc taatagttac
     7561 caaaaaaata tttcaattgc tgcacaagct gcatttttaa cttctgtaaa tccagactac
     7621 attaaaaata tgatgaaaga tggaatttta atgtttggag catatccgaa ccaaataagt
     7681 gcagcagcag atcaagtaac aacagcaatg agtaatttac ccaatataga tttaccaaaa
     7741 ttaagtggtg cttctgagtt ttctactcaa acagcattat atgacgcttt tttaaatgtc
     7801 caaaaagcaa aagattttgc aaaaaacgga gctttatctt ctaattccgg tattcataat
     7861 ttaggaaatg cagaaacaaa ttctacgata acaggatctt tattatcctg gtttacacaa
     7921 gataataatt taggtattgg caatgacgaa ggagcaattg cttctaaaaa tctattgtta
     7981 gctgctaaat cagaaggaaa caaactaatc aatgcagcct gggcctcgtt cgctgctgct
     8041 actggttttg ctgttgcagg tggtgctgct tcaagcaatc ttatatcgag tgttgtaggt
     8101 gctgacggtg gtttccagag ccttatgcaa atgttaaccc ctgtgtttat gactatcttt
     8161 ttctcacttt taggtgcagg tattacatta gctgtattca ttccactttt agtttttata
     8221 acctggtcaa tggcaatcat tcactatgtt ctggaagtct gcctggctgt ctgtgcggct
     8281 ccattctggg ctttaagtca tacagtagat aaaggcgaag gattgatagg aacggcttca
     8341 aaaggctata cagagcttct atcacttgta tcaagacctg ctttaatggt tttaggtggt
     8401 gctttttcaa tgaaactttt atcagccgtg aatggattta taaatgaagg ctttgcagca
     8461 gtatttaatc ttaatgttat aagcgatacc tctggactcg gtataactat tttctgtgga
     8521 agtattgttg tttattcatt atgtatgctt gctcttacta aaaaaatcat atcttgcata
     8581 actcttttcc cagataaatt tatgacctgg ataggcggtg ctactggcga tatgtcttca
     8641 ccagttaaag acattggtgc aactacagca attgctgccg gttctactat tggagcaatg
     8701 aaagtaggta atggttcaag tttggcaaat gtagctaaat caatggctgg ggggaataaa
     8761 gcaaaaccta agagtcctgt aaatacacca aattttgata atgcaaataa tacaagttca
     8821 ggatctgcaa aagaaaacaa tgctgaacct aacaaacaag catttaagca tttaaaagac
     8881 ctaaatagag ataattgata aataacatag ccagtgtaaa ggctggctat attaaatttg
     8941 acaacgcatt tttaatatgt aaatcattaa ccaaaataat ataaaaaggt gaatatatgg
     9001 aaagtaaaat ttttaaaaat cttcaaatag ctgtatttag agaacaagtg atgaatataa
     9061 aaaatcacaa caacattatc gatattgtag aaacaatgaa agataaagta tctttcgata
     9121 ttttaattta tcattcagtt atagcttata tgtcaaaagt cgaattgtta gctttacaat
     9181 acagtcatta cattgaaacc aaaaaatcta ttacagacag tattaaaaat gtcagggatt
     9241 ttatttgtgc ttatgagaag aaaagacaga aaaaagttaa agatatggac tataacgaat
     9301 taaaagaaag gcaaaaatat aaagagttta aaagaaatat tagcttaata gataatatgt
     9361 cagatctgta ttcaggcaat gataaagagt tttatcacga aatagcagag caattaaaaa
     9421 gatatgacga tttaatttat catttacttg aacataaaga ctgtaattta acagacgata
     9481 attttatttc gttgatagaa gatatgaaga aatttaaagg gaaaaggaaa atgaacaatg
     9541 aaatatttga aagtttgcat actaaatata taacaatact gtctgataga gtgttgcatt
     9601 tagtattcga aaatgcagaa cctaaattaa aggggagggt gtaatatgga tataatatta
     9661 acaatcttcg tatacatacc agccgtattg ttttatatat tgctattgag tgaactatat
     9721 aaacttatat taaaaaatat caaaagaaaa ataaaaagga aagttaaaac agaaatatta
     9781 gatagtttca gaaactggaa aggttcttta aacgaattcg aagaagaact tgaaacttcg
     9841 gttactgatt actttgactc tgaaaatgac ggtttaacaa cagcagaaat aacagcaagt
     9901 gatttagtag catatgaaga caatagaaaa agaaaataaa taagccccgc aatggggttt
     9961 attttttatt aattattttt aaatatgcta ttgacaataa atttagatcg aatataaata
    10021 gtatatagca ctatatagcg atatatattg atatatacta aattcaacaa ggagaattaa
    10081 ctatgaaact ttttaaaaaa actattcaaa aaaacaaaga tagaattacc aataacggaa
    10141 gcttccagat attaaaatat ttcttttatc cacagaaaag agaagaaaga aaatatagtg
    10201 gttttgcgga agcattagca gactacaaca cggacgaagc aacattgcaa aagagttata
    10261 gaaacttttg ttttatggct tatatgtggg gctttgcttt cttagcaaac ctattttatc
    10321 tgttaatggg tttatttata gacggtcaat ttcacgcaag taatttcttt tttggctgta
    10381 tctgctttgg ctgttggttt aaaggggctg cctatgctta ctcacttaaa tatcacacat
    10441 tagaagtgat aagagtatta atgtctcccc tggactggtt ccctgacttc acacacaaag
    10501 taaaaatggt ttacaaagat aaaccagctt ccagaaatat agatattaga agataacaaa
    10561 ataaggggga aaccccttat aaggagaact ttatgaaaga tagaataaat aaaatattag
    10621 ataattcaat caatataaca atcaagataa taataaatac tatatttgct tttataagtg
    10681 ttaatataat atgtatatca atgtattgtg ttataaagtt gatgaattgg ttattttttt
    10741 aaaaaaggag tatctcaaat ggaaaatgaa ataataaata tacattttaa attgccgggt
    10801 caatttatta ctgataatga tttagcaaca atgtttaaaa ggaaaatagt agttataatg
    10861 gaccgtagag gaactggtaa aacaactatt ttaaaaaata tttccaacaa attaacatta
    10921 ccttcaaaat cggtatttat agacgattta tcgtttaatg gaaatgtaaa taaaaggctt
    10981 ttaaggaata acatacaaaa ttatataaaa aaaggtgtat ttgtttttat ttcgacaagc
    11041 tgtaattatt ctgttgtaaa ttattattta aaaccacacc ttgattatat tatgtatgac
    11101 tcagtatttt tgtttgacgc tttaaatgga ggtcttggag actcaacttc tctttatgtg
    11161 gggtcaagag aagaatgcta tgaaagattt ttaaaaggcg aaataactaa cgaaaatgta
    11221 gatactgaaa aaactgatat gaatatcatt aatgagttta tagcagagcg agataaaaca
    11281 cagatcacag aaattttaaa atcaaaacaa gtctacaaca aaaggcgatt ataaaaggag
    11341 atcggaaatg aaaagagcaa ttataaattt aaacaacaaa atcttactta gtgatatttt
    11401 caaaaaagaa attgataaca agattgaaaa aaattattta actctccata attacattac
    11461 taatggtgat ttatttaaaa gagaagattt taattcttta cttgaaagga gaatgcttaa
    11521 tttaggtctt tacgacgaaa aacaattcag acaatctttt aaagactcac ttcttataaa
    11581 taatctaaaa aattttgtgg gtgatatgtt ctcacattca aaatattcaa acctggagca
    11641 ttcactacaa gatactatca ctaatagaca agtgttagta tcagaagaag attttaaatt
    11701 aactaacgat aaaatagtta actcaatcag gaacaataat cttaatggtt ttcattgtat
    11761 acttcatata aatacaatgc ctacggcagt ttattatgta tcacataaaa atgatattta
    11821 tgaaaatata gagaaagttc aaagagaagt tatagacagt gagaaagaaa aaattgaaaa
    11881 ggcaaggctt gatctgttag atataaaatc aaaatactac gataaattta ataaaggtga
    11941 gttagatata ttaattaatc ctgtattgct aagtgaattt ttagttaaaa aagaaaatga
    12001 agttttgact gatattttag atagtaaaca aacaagcagt aaaagaagac tataaggaga
    12061 gatttatgaa aaataatgtt agatataatt gcaaagcaat attagaagag ttaaaaagtg
    12121 atggtgtttt ggattcattc gttatctggt cgtcaagtat atatccagac atccatttaa
    12181 gtcagtcttg ttttgaaagt tgctatgaaa tatataaatt gaacgaaaat ttttttaaag
    12241 aaaattggaa taaaaaaata atatcttcct aatagaaccc ccagccctgg gggtttttct
    12301 tgcttgcaag tcctacatgg taggactata atcaactcat acagggtagg agttacaagc
    12361 aatggaatat ttggaattcg ttgaaacttc tgtttttacg agagaatgta aaaccttact
    12421 cactgacgac gagtacaagg agtttcagac tcacttactc ttagacccgg aagctggatc
    12481 gatcatagta ggaacgggcg gttgtcgtaa ggtgagatgg gccagacagg gcacaggcaa
    12541 gagttcgggc attagagcta tatactacta ctacaaccca gcaggacggc tctatatgct
    12601 tcttgtctat cctaagagtg agaaagacag cttatcagca gcagagaaga accagcttaa
    12661 agccgtggta tccgttttta aggagtcata gactatgaga aaagaattgt ttgaagagct
    12721 cattgcttct gcaaaagaag cggtagaaat ccaccagggg attcgtgaac ctgctcgtat
    12781 cactcggatg gccattccag atgttaaggc tatacggagc aatacagggc ttaaacaaga
    12841 cgattttgct tcactggttg gagtatcccc agctcttgtc caggcttggg aacagcaccg
    12901 caggacacct tctggaagta gcttaaaatt acttcgtatg attgagttaa aacctgattt
    12961 aattgacaca ttgaagttag cttaaataaa taagaaagcc cctgactgac aaacaggggc
    13021 ttaataaaca caataaggac ttttcgtttt tacctttatg tacattcctt gtacacatat
    13081 aaacacatat caggttttta actattgtca atattaaata gcactttttg taattacaca
    13141 gtaatatcat caacttaaat aacctttttt attacgaaaa cgcttgcttt tattttccaa
    13201 ataaaaataa ttctgtactt cttttatttt ttaatatatt gtcattctat taaaacaata
    13261 aaggagaata aaaatgaaaa aacaaaacac tcaatactct acattcactt catatctttc
    13321 taaaaccaat aagaatgtag atatgtatag actctataac ccaatgtttg ataacttgaa
    13381 ttatttcggt ttcgcatatg atataaattt ttatggttca tattttaaac gacataatat
    13441 tacaaaaaga aacttcttaa ctattttttc cttttattgt tataaactaa gtaatgaaag
    13501 atttttaggg aataaagaaa tatatatgga agataaaagc ctttttatta aggcttcaat
    13561 gaaattattt aaatataaaa ttaaacactt atccgataat attgataatt taaatatttc
    13621 tttcaggggt ggaaatgtga tttttatttc acaattcggg aagaaagaag aaatttcata
    13681 tttcacttta tcaaaaattg caggtgatat ttttgatatt aaagactttt caatgagcaa
    13741 tgaaaaatta cttgcggcat aaatttaata aggataaata aaatgaaaaa acaatttaag
    13801 aaaacactgg aaagcattca aacacgatct aatcgttcaa tttctaatac agttagggag
    13861 ttctttaatt gtgtatcata tacaatgatt ataaatagct tttttcaact tgaacaaaga
    13921 gaactatttt atttaggtga taacattcat agtattaata aacttctcac gcaatcaggt
    13981 ttaatcaata aatcaataac attgacagat agacacgaat ttaaaataga agaaaaattt
    14041 tacaatcagt ttgagagaga aataatagaa atcgccaatt tgctttctat tgaaataaat
    14101 aaaaaccctt taacagacat attcaatgag ttgtttgaag agtgttattt atctggtaaa
    14161 aaaggaggtg gttttgctca atatttaacg cccaatagag tgtcaatagg ggcggtaaaa
    14221 tttttagcat taacaaaaaa agaaaaatat catatagggg atatttgcgg ggggacggga
    14281 tctatgattt tagcattagc ttctgaaatt tataaaaaag aagggttgga aggaattcaa
    14341 aaaatagact tattttatca ggacaaagat cctttcattt ctcaaatatt cattgctcaa
    14401 attttaacga atatgattta tcataatttt gattttaatg aattacgagt ttacataggt
    14461 gattctataa ctgagtacga tactataaac accctatttt taagagttaa acaaaataaa
    14521 ttagtagctc aacgggtatt agaaattgat aaagaaaaga aagcagcgta attctaaaaa
    14581 ggataaacaa aatgaaaaaa caattttata aagcaatgga aaatatttta acacgatcta
    14641 atcgctctca ttcggataca gtcagggagt tttttaattg tgtgtcttac accatgataa
    14701 tgaatagtct ttatcagtta gaaaaaagag aacagtttta ctcaggtgaa acaacatatc
    14761 ggttttcaac cattctcaat gaattgcatg tagcagagag aaatataatc ataacaaata
    14821 ggcataaatt aaaaattgat aatgtatttt ataaaagatt cgcaaatgaa ataaaattta
    14881 tttcaggagt attatctaat gaaattggga aaaacccact cactgatata ttcaatgagt
    14941 tgtttgaaga tttattttta acagataaaa aaggggacgg gttcggtcaa ttccttacac
    15001 ctcatgatat atctgaactt ttgacagata taatttatac tacaagtaaa gataaatata
    15061 aaatagctga tagctgtgct ggtactggat ctttgatttt tcctttgctt aaaagaatct
    15121 ttttgaaaga aggttttgaa ggtattcaaa aagtagagtt gttttataat gataaagatt
    15181 catttgtatc tcaattattc atagctcaaa tactgactaa tatgatttat cataatttag
    15241 attttaaaga tcttcgtgtt tatattggtg atgctataac tgaatacgat actataaaaa
    15301 ctttattttt aagagttaaa caaaataaat tagtagctca aagagtatta gaaattgata
    15361 gagaaaagaa agcagcataa taaatataat gagaaacccc taattaccaa acggaacaaa
    15421 ccaccgggaa agtaaaaagg ggatctcaac tggattatat caaaaaaatc ttaacccgaa
    15481 ccttct
//
