LOCUS       NZ_AP027511             2139 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-27 plasmid pLR-27_5, complete sequence.
ACCESSION   NZ_AP027511
VERSION     NZ_AP027511.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562507
            Assembly: GCF_036495405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 122x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036495405.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:15:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,414
            CDSs (with protein)               :: 4,414
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 77 of 279
            Pseudo Genes (incomplete)         :: 189 of 279
            Pseudo Genes (internal stop)      :: 59 of 279
            Pseudo Genes (multiple problems)  :: 41 of 279
            Pseudo Genes (short protein)      :: 3 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027511.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-27"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-27_5"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            join(1414..2139,1..270)
                     /locus_tag="AABK22_RS24065"
     CDS             join(1414..2139,1..270)
                     /locus_tag="AABK22_RS24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_038989441.1"
                     /translation="MQNPKLKNLTDYSPDDKPWDVHKSQSDDVGGIYLRAAEFEAYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDARWMFLTLTVRNCAIEDLGDTLTAMNAAFQRMKVRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPGMLNGHDYVKHYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     Typing          complement(2..181)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.425"
                     /coverage="92.0"
                     /evalue="1.84e-38"
ORIGIN
        1 tattcgacaa agccggctga tatggttgcc gacccggact ggttcttaga gctgacccgg
       61 cagacgcata aacgccgttt tgttgcgacc ggtggcgctc tgaaagacgt tctgaaactg
      121 gagcaggaaa cggatcagga catggtgatc ggggatgata tttccgatgg tgaagacgat
      181 ggttcacgcc tcgcctttgg ctgggattcg acgccaagaa aatacgtccg tttgcctgaa
      241 cgcgataagt ttaaatcaga tcctgattag ggtgtcgggg cctgccctga ccaggttgtt
      301 tttggatgcc gctgcgtgtg gcggtactcc aaaaacacat cttgtccggt acacagattt
      361 cctttggcat tccttcgggt ttccctgaga tttccactct gcatacatac acgcatacac
      421 gaataatcaa cggcagaatt cgggcaggat atgtaaagtg ggaccacttt acgcctgcct
      481 tattcgcact ctgctcaatg gaatattaaa tctgacagcg ttcgccgccg tcgaatcgtc
      541 aacttctaaa tcgggggatc cctttagggt tccgatttag tggttgtacg actgcacccc
      601 tcataccctt gtgatgggtg atggttcaca tggtgagcca tcgccctgta aagccgcttt
      661 ttaccccctt caaggcgttg cctgtggggt aggtgcgtaa gccctgccct acaggtgatg
      721 ccgtgggggt attttcgttt tgctcggtat gccttattcg tttaaatccg ccatcaggag
      781 tatgcggggt aaagtcgtgc ggccgcgatg aggaacgggg attgcccccg cagctctgcg
      841 aggaggcaca cgccgttcgg aatcggggtc cactctgttt aatagtggac tcttgttgcg
      901 atttgcaacg gaaacagccg cgtgcagtga atgtaatact gcacgtttgt ctcaatctga
      961 catttctcag ggggggggcg atttaacgtg gtttgcttaa tttttgagct tacgaatgct
     1021 tgagtgtttg agtgcctgaa tgctgctttt gggggttggt ttttcggccc tgaaaggggc
     1081 gaaatccttg tatttatact taagggataa ctctaggatt caaattcgct tccagccctt
     1141 gtgccgcaag gcctgagagc caatttgctc gaaaatggtt ttgtgagtat gatttctgac
     1201 aggtgatcgt gtccgcgaac taatttttga ttacttgggg tggttcccaa gaccttactt
     1261 actgacacga tgatatgaga aacccctgca agtgctacca acacttcagg ggcgcgatca
     1321 cctggctaaa tacggtttga aatcgactcg caacaggtga tacggaaatg ataacacacc
     1381 cttccagcgc ctgcccatat tggagcaggt gctatgcaga atcctaagct aaagaacctt
     1441 actgactaca gtcccgacga taagccgtgg gacgttcaca agtcgcaatc tgatgatgtt
     1501 ggcggtatct atctgcgtgc agctgaattt gaggcgtatg cggctcgtat gcgcgattgt
     1561 ggcggcttgc tgcgttttgg ctggtctacc cttaaagaca ccggagaaac gcgtttacgg
     1621 cttcgtgagg cgcatttctg ccgcgttcgt cactgccctg tctgccagtg gaggcgttcc
     1681 ctgatgtggc aagcccgttt ttatcagtct ctgccgaaaa tcgtcgctga ttaccctgat
     1741 gcccgctgga tgttcctcac gctcaccgtt cgcaactgtg cgatcgagga tctcggggac
     1801 acgcttactg ccatgaatgc cgcattccag cgcatgaagg ttcgcaagga atttaaatcc
     1861 gtacagggat ggattcgcac cacagaagtt acccgcggtc gtgatggctc tgcacacccc
     1921 cactttcaca ccctgatgat ggttcctcct gggatgctga acggtcatga ctacgttaag
     1981 cactaccgct gggttgaact ctggcgtgac tgcctgcgcg ttgattacga tcctaatgtt
     2041 gatgttcggg cagtgaaacc ccgcaagcct aaagatggcc agactctggc atgtgccact
     2101 gctgaactgg tgcgcggtgc cgttgctgaa accctgaaa
//
