LOCUS       NZ_AP027510             2390 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain LR-27 plasmid pLR-27_4, complete sequence.
ACCESSION   NZ_AP027510
VERSION     NZ_AP027510.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562507
            Assembly: GCF_036495405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 122x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036495405.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:15:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,414
            CDSs (with protein)               :: 4,414
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 77 of 279
            Pseudo Genes (incomplete)         :: 189 of 279
            Pseudo Genes (internal stop)      :: 59 of 279
            Pseudo Genes (multiple problems)  :: 41 of 279
            Pseudo Genes (short protein)      :: 3 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027510.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LR-27"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLR-27_4"
                     /geo_loc_name="Ecuador:Santo Domingo"
                     /collection_date="2019"
     gene            complement(3..116)
                     /locus_tag="AABK22_RS24045"
     CDS             complement(3..116)
                     /locus_tag="AABK22_RS24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            687..836
                     /locus_tag="AABK22_RS24050"
                     /old_locus_tag="VEE09_46060"
     CDS             687..836
                     /locus_tag="AABK22_RS24050"
                     /old_locus_tag="VEE09_46060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_169051925.1"
                     /translation="MSSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1602..1841
                     /locus_tag="AABK22_RS24055"
                     /old_locus_tag="VEE09_46070"
     CDS             1602..1841
                     /locus_tag="AABK22_RS24055"
                     /old_locus_tag="VEE09_46070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560795.1"
                     /translation="MIKKICITVIVVFLLLVGYGAWIGSEQNQRGVSLFEVAYTYNAMN
                     PISRIGYTFMLKRNHALVERAGEVKKSIDSMSGE"
                     /name="hypothetical protein"
     gene            1865..2164
                     /locus_tag="AABK22_RS24060"
                     /old_locus_tag="VEE09_46080"
     CDS             1865..2164
                     /locus_tag="AABK22_RS24060"
                     /old_locus_tag="VEE09_46080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016247612.1"
                     /translation="MKKILFCSLLLLSASSFAQQFHYQCEGRDLKIMLSPNAAFLDGKS
                     FEYKNGETNGETTKMTFFGPSGEVLKFLVNPVNNLYRMGLTSGATFEEINCTEI"
                     /name="hypothetical protein"
     Typing          479..652
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(8..555)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.304"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca
       61 agacgatagt taccggataa ggcgcagcag tcggactgaa cggggggttc gtgcatacag
      121 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg
      181 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
      241 ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccgccactg
      301 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggcag
      361 cgccgcggcc ttctcgtttc cctgttaagt tcgctcctgg catctacccg gaaatttccg
      421 ccccatttgt aagccggtac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg
      481 agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt
      541 tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca
      601 gtatacactc tgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgctaaca
      661 acctctccgc tactggatat caccccatgt catctgaata tgatgaacaa ctggattcga
      721 ctgatacgca gcttcccggc gaatatgacc tttttgattt aatggcgcag gttactgaag
      781 agaacctgca cgccccggtg gattttgggc cgcctgtcgg ccgggaagcg ttatagcttt
      841 ttcgcttacc cgcgtacgtg taatacgccg gtattacatc ttatttcttt attttcatgt
      901 gattacaggt ggttgtaata cgttgcgtat tacataaata aacgggaatt tcagttttta
      961 ccttttaatt catgcaattg cgatgaaaat cgcggttgcg tatggtgtat ggccatttaa
     1021 gggataaaga atcatctcct taccgggctg agctttgcct attctgagga gatctgtgaa
     1081 aaaaagcgtc cataactagc tcgttcatac ttgtatgaac agcttattcc tcctgactac
     1141 taataaacta cagcgtcact actgatttac tactgattta ctacaaaccg gaggggggga
     1201 ctacagaaac actactgatt tactactaat ttactactga attacgtcat aacagttctc
     1261 tgaaccattc gtacatccga cccccttctc ttcatgcttg atgttcttgc caacgcttca
     1321 cggtcacgca ataaattgcg tccccgttca ccgttttcgg gtaagaagaa cgccggaaac
     1381 aggacttccc ctggctgaat ggtgtgaaat tctgcgctat gcacttgcgc gcatactcat
     1441 gcatgcgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt
     1501 tttaccggtt tcccgtcaga aacgccctga gggcctctca ggcggtgcac gcaacggatg
     1561 ttatgttcaa ttctgttttc ttcatattat ctggataggg aatgataaaa aaaatctgca
     1621 ttactgttat cgtcgttttt ttgctgttgg ttggctatgg tgcttggatt ggatctgagc
     1681 aaaatcagcg aggagtatct ttatttgagg ttgcatatac atacaacgca atgaatccaa
     1741 ttagtcggat cggctataca tttatgctga agagaaatca tgctttagtc gagagggctg
     1801 gagaggttaa gaaatctatt gatagcatga gtggtgagtg attttttaaa tttaaaggac
     1861 tgatatgaaa aagatacttt tttgttcgct gcttcttctt tctgcaagtt catttgcaca
     1921 acaattccat tatcagtgtg aaggacgaga tttaaagata atgctttcac ctaatgcagc
     1981 ttttcttgat ggtaaatcat tcgaatataa gaatggagag acgaatgggg aaacgacaaa
     2041 gatgacattc tttggtcctt ctggtgaggt tctcaagttt ttggttaatc ctgtcaataa
     2101 cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat
     2161 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
     2221 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
     2281 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
     2341 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta
//
