LOCUS       NZ_AP027261             6675 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia coli strain JNE151685 plasmid pJNE151685-3, complete
            sequence.
ACCESSION   NZ_AP027261
VERSION     NZ_AP027261.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00566466
            Assembly: GCF_030295645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Complete Genome Sequence of Eight LEE-negative Shiga Toxin-producing
            Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome
            Patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2023) Contact:Akiko Kubomura National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 108.6x
            Sequencing Technology             :: Illumina MiSeq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 12:21:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,138
            CDSs (total)                      :: 5,011
            Genes (coding)                    :: 4,858
            CDSs (with protein)               :: 4,858
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 62 of 153
            Pseudo Genes (incomplete)         :: 91 of 153
            Pseudo Genes (internal stop)      :: 33 of 153
            Pseudo Genes (multiple problems)  :: 29 of 153
            Pseudo Genes (short protein)      :: 3 of 153
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027261.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNE151685"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJNE151685-3"
                     /geo_loc_name="Japan"
                     /collection_date="2015"
                     /note="National Institute of Infectious Diseases JNE151685"
     gene            complement(59..382)
                     /locus_tag="QUE29_RS25645"
                     /old_locus_tag="JNE151685_48940"
     CDS             complement(59..382)
                     /locus_tag="QUE29_RS25645"
                     /old_locus_tag="JNE151685_48940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            450..641
                     /locus_tag="QUE29_RS25650"
                     /old_locus_tag="JNE151685_48950"
     CDS             450..641
                     /locus_tag="QUE29_RS25650"
                     /old_locus_tag="JNE151685_48950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(793..981)
                     /locus_tag="QUE29_RS25655"
                     /old_locus_tag="JNE151685_48960"
     CDS             complement(793..981)
                     /locus_tag="QUE29_RS25655"
                     /old_locus_tag="JNE151685_48960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(1688..1834)
                     /locus_tag="QUE29_RS25660"
                     /old_locus_tag="JNE151685_48980"
     CDS             complement(1688..1834)
                     /locus_tag="QUE29_RS25660"
                     /old_locus_tag="JNE151685_48980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            complement(1904..2167)
                     /locus_tag="QUE29_RS25665"
                     /old_locus_tag="JNE151685_48990"
     CDS             complement(1904..2167)
                     /locus_tag="QUE29_RS25665"
                     /old_locus_tag="JNE151685_48990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            complement(2164..4257)
                     /gene="cda"
                     /locus_tag="QUE29_RS25670"
                     /old_locus_tag="JNE151685_49000"
     CDS             complement(2164..4257)
                     /gene="cda"
                     /locus_tag="QUE29_RS25670"
                     /old_locus_tag="JNE151685_49000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            4521..4766
                     /locus_tag="QUE29_RS25675"
                     /old_locus_tag="JNE151685_49010"
     CDS             4521..4766
                     /locus_tag="QUE29_RS25675"
                     /old_locus_tag="JNE151685_49010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            complement(5256..5489)
                     /locus_tag="QUE29_RS25680"
                     /old_locus_tag="JNE151685_49030"
     CDS             complement(5256..5489)
                     /locus_tag="QUE29_RS25680"
                     /old_locus_tag="JNE151685_49030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137424.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVLRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5496..6056)
                     /locus_tag="QUE29_RS25685"
                     /old_locus_tag="JNE151685_49040"
     CDS             complement(5496..6056)
                     /locus_tag="QUE29_RS25685"
                     /old_locus_tag="JNE151685_49040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001345151.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6088..6525)
                     /locus_tag="QUE29_RS25690"
                     /pseudo=""
     CDS             complement(<6088..6525)
                     /locus_tag="QUE29_RS25690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(727..810)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="1.15e-36"
     Typing          complement(1272..1459)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="100.0"
                     /evalue="2.18e-82"
     Typing          complement(63..490)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.811"
                     /coverage="100.0"
                     /evalue="9.59e-78"
     Typing          complement(5150..6675)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.411"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ccggcccagc aggtaatcaa ccggaccact gccaccgcct tttcccctgg catgaaactt
       61 aactatcatc gcgttctccc tgtttacgga cctcatccct cagctcactc agttcacgtc
      121 cgatggccat cagtgcagcc accacatgaa cccggtcatg ccccgaccac tgtccactgt
      181 ttatcttccg ggctatctga ttcaggttat tgccgaccga agcgaactgg cgcaacagcg
      241 gcggtgccag tgtcggaaga cctgacgttt tcgatggcgg tgcccccagg cagaccttac
      301 gcatccatga cgcaagttgt tttccctcac aacgtgccag cagccgcgca tgttcctcat
      361 ccgtgacccg tatcgtgagc atcctttcgc gtttcaccgg tatcattaaa aacctccgac
      421 agactcccca cacatggaga aacagaactg tgactaaaca ggaaaaaacc gcccttaaca
      481 tggcccgctt catcagaagc cagacgctga ccctgctgga aaaactgaat gaactggacg
      541 ccgacgacca ggctgacatc tgcgaagcgc ttcacgacca cgctgacgag ctttaccgca
      601 gctgcctcgc acgcttcggg gatgacggtg aaaacctctg acacatgcag ctcccggaga
      661 cggtcacagc ttgcctgtga gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      721 cgggttttag cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
      781 atactggctt aatcatgtgg catcagtgag gatggtatga aaagtgcacc atgccgggtg
      841 tgaaatgccg cacagatgcg taaggagaaa atgctcgtcc aggcgctttt ccgcttcctc
      901 gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtatctg cccactcaaa
      961 agcggtgata gtgttatcca cagaatcagg ggataacgcc ggaaagaaca tgtgagcaag
     1021 aaacgaaaac cccgaaaaag gccgcgccgg aggcgctttt tccataggct ccgcccccct
     1081 gacggacttc acaaaacaga cactttttcg gtgcatctgt gggagccgtg aggctcaacc
     1141 atgaaatccg ccagcggtgg cgaaacccga cgtggcttaa agatcatcac cgtttccggt
     1201 gaatcgctcc ctcctgcgcc cccacgttcc cgccatatcg attaccggat accttttccg
     1261 cctttctccc ttgcgggaca cgtggcgctt tctcatagct cacgtcttct ggtatctcgg
     1321 ggcgacttag gcagttcgct ccgggctggg ctgtatgcag gaaccccccg ttcagcccga
     1381 ctgctgcacc ttctccggta actgttctct tggggccaac ccggaaaaga cacgacttaa
     1441 cgccactggc agcagccatt ggtaactgat ttagaggagt tgctcttgaa gttatgaacc
     1501 agttaaggct aaactgaaaa gacagttttg gtgactgtgc tcctccaagc cagttaccac
     1561 cgccgattag aagaggtcga agacttctga tcggacggaa ggacccgcca caaggcgggt
     1621 tttttcgttt acaggcagca gattacgcgc agaaaaaaag gatctcaaga agatccttta
     1681 atatttttca ctgaaccgcc accccgatta gtttcgaaga tgatgatgga gcaattgttc
     1741 caccctgaac atcccttaca tagttagctt ggcaagcagt cagacacaaa acagcaagaa
     1801 cgagaatcat agatccccct atcttttttt tcatgacatc tccttgtcat gagtgaatat
     1861 ctgacattta tacttaaatg ccagatatta aaatcactca tgattataat ttaaattttt
     1921 ccaagataga tcggacctca accttaagtc cattatcatc aatttcataa tcagccctat
     1981 ctgcatcagg ctcaaatcgc tcagcagcca taaacaactc ttccccacaa tttaatatat
     2041 tcggagacaa atccttaaca ccatacaatc ttctccgttc aacacaaatt cgctctgaaa
     2101 actcaattgc tgtaattttc gaagctaaaa ataatttcgc caaatcgatc attgccatct
     2161 tgttcatagt tcacccgtct ctaaataaat tcgaccatta tccgcatctg gatttatttt
     2221 ccacccagat aagaactcac catttgactt aattataacc acattcatcg tattaggatt
     2281 aaaataaact tttgaacctt tttctcttcg gtatgttcct ttctctactg tatccttatc
     2341 cgataaatgc tcctcaatag catccctgaa tttagtaaga gtttcacgat tcttttttgt
     2401 atcactaata ccaaaatcac cagcatgttt atatttttta tcaagttgtt tacgcgaaaa
     2461 tcgccctgaa tccagaggat cattaagcat cacataaaca gcttcaacac cagtcccagc
     2521 agcatcagga cgccagtaaa taaaatctcg tagtccacca acatcagaaa caggagtaat
     2581 cgtcgttact ggcatttcga ccggttttac ttccgttcct gtatgcactg gtgtaaccgg
     2641 aacgggttga tgattacctg tatctgttgg caccgaaggt tgtggtaccg gattaaccag
     2701 aatcgtccgg gaaggtgcgc ctactactgc tggtaacgtg attttatcca gtcctgttgc
     2761 tgtatcacgg acagcattaa gaacaggaac cgcagctggg atactttcat tccctgtttt
     2821 cagtaaatcc agagctaatt gcccgttact gtttatcaat tgtccacgaa caggcagatt
     2881 tacactcgtc gcaccaggtt caatgaccac gccctgtcca gccaacatct gagcactcag
     2941 cgaaaacatc gcctccagat ttcgccccgg cacgatatca cttcctgctc cagcttcttc
     3001 tgaatgaaac cacagagcac caagagaaat ccgcataagc aggggactca cttcgggtat
     3061 ggttactttc tccagagcat tactgaaaaa cttgcttatg ttgcctgcaa tatcaccagc
     3121 agcagtccag acaccagttc cggtcatcgc cagttgtaca gaactggcag tgttattcag
     3181 aacaagagct ccctgctcat tcattactga attagccctt tccatcattt cctgtgtata
     3241 tacaggaggc tggttcacac ctgctttact aaataactcg gcttttgctt tttcagcttc
     3301 agcccgggct accgcttctt ctttcttgac tctgaaatca tcctttgctt tattttcggc
     3361 ttcctgacgc tgactcagac cattactgtc cagaatctct acagttgaaa tatataacgg
     3421 agaaacacca ctgccatcag gaaaacgaac aatgacatca tgattattac tggacatcgt
     3481 ataaccagcc tcaacataat tacctgtctc agcaataact ccttcttcct gaatttcggg
     3541 attagacaga accggagtat tcatctgacc cacacttaat accaggtccg gtagatttgg
     3601 tgatacaggg gcactgtaaa catctttaat atttgttccc ggacgactcc ggtccggaac
     3661 tggaacggca tcaactaccg gcactgtcat cggaatacct ccgataatag ccatatattg
     3721 cgtaccgttc tgcataaggt ccatcagacg atatttcacc ggtacattat tacccgctgc
     3781 tggtaactgg ctggccggaa tatccgttac ctgttccagt tgctgtgtaa ccataagacg
     3841 aataattttt tgctcatttc cggaagcagg ctccagacgg gcaaaagaaa cagcagccgg
     3901 gcgagacggt tctggcaaac caccacgtaa accattctca agaactgttc gtgcagcgga
     3961 aactttctcg tttccccaag ggctatgcac actgacagac catcccgacc agtctccctc
     4021 ttcaagttcc ttatccagaa taatccggcg aataattccc ggaaaaagaa cactttgtac
     4081 aagtgcgata ccctgtgggc tgtaatcccc ccatcccgga ggcatggatg gagcgatacc
     4141 tgatgagcct cctggtttca catcaccacc cgaaatcagt cctgttgacg gccacacgac
     4201 catcgagtgc ccataatcaa taccttctgt cggaccacta ccttcgtaat cactcataaa
     4261 aacacctctt tgacttttaa aacaatgcgt taaaaacaac aaactgtaca taaaaccact
     4321 ggttttatgt acagtaaaaa gttacgtcac ctcgtaaatc ctgtcaaccc actggattta
     4381 cgtttttgtg aaaacgctca agaaaaacaa ccaatcattc ttaaactcaa tggcaacagg
     4441 catgaaagaa ggctgattag cataaggaga gagccaacag ttaaactgtt atgacaccag
     4501 agaaacaccc ggagaccacc atgaaacgcc atagactgac cgaagccgaa gagcgccgac
     4561 tggcagaagc cattgccgct gaagctcgtg ccatggggct gacgaaaaag gacaaaatgc
     4621 cgcaggaaac aaagcgacgc ctccgggcac ggatacttaa agccattctt gatgaacgaa
     4681 cggccagcaa aaatcgagaa gccacactga ctgccggaca ggacccggat gacaccttca
     4741 cctggcaacc actgcgccgc cgctaagcta agggttattt aacataaccc cttattgtac
     4801 gcaccgctga aatgcgttca gcgcgatcac ggctgctgac aggtaaaaat ggcaacaaac
     4861 cacccgaaaa gttgccgcga tcgcacctga taaattttaa ccgtatgcat agctattcag
     4921 ccatgtgaat aacgctggtt ttgcctgcgt aaacctcatg acactgtttt ttttccatct
     4981 tttcagttga tgacatacgc agacatcgcg ggatgaggct gaggaatgag cgcgatctgg
     5041 caaagaggca aaacacagca acaaaaacga cacgccagaa tcgcgcccgg atgcgttttt
     5101 aacgcgttcc ggtaccatct ggcaacctcc cggaacaact caccgtcaca tccccattga
     5161 cgggccacgc catacccgtg cttcccgttc ctgctcttca tgccaggacc gcgcacgctc
     5221 ccgttccagg cgtgcctgcc tttcctgttc atcccttatc tgctgttcgt gataaataac
     5281 cgactcaagc ggtccacctg cccggctaat ctcagcacct gctgactcaa gtcgtcgcac
     5341 tgttccctca gttgcccgtt ctcctgtcgt gtcagctcga acatatgctg caaatccgtg
     5401 aaggcgctct cccagtcttt cagccgctgc gtatagtcct gctgcaattg ctctaaggcg
     5461 ttcagtaagt gcatttccag ctctgtcata ctcacttact ccctgaccag tcttactgcg
     5521 ttcttcttct ccaccgtcca gttgttttcc cccttcaccc cggacggcaa caccagaaat
     5581 ttcccgttcc tgccctcgtg atacgtcaca ccccatgttt tttcccggag tttcgccagc
     5641 gtctcttcct ggtccctgat agccaggatg ttcgccgcaa tacggctttc ctgccactga
     5701 atcagccccc atgacgccag aaaaatcccg accgtgacgc aaagcgccgt cagcgacggg
     5761 tacagtatcc gccctttgac cagcttccag agcagctctt cctgccgccg ggcaagttcg
     5821 ttctctgtgg cgctgaactg cgcgttcacg gcactgttca gcgtctccag ttgttctttc
     5881 accgctgctg tgtgtgcgct gatagcgtct ctgattttct gcccgtttaa gttcagttcc
     5941 ctgtctacag acgcttcgag cttcctgaac tcgctgttca gcatgttctc tgtagagacg
     6001 gcacgctctt tcagtttctt ctcgaagtct gtccccattt gtaaaagatt gctcatacag
     6061 cgcccctttc agcctgagat tacgcccgcc ctccgggtcg gcgatactga tactgctcct
     6121 ggttgtcctc accacctcaa aacctgccgc tgtaagcgcc tcagtgacat cctgacgcgt
     6181 ttttagcgct ccggcatggt aaagagcctc cagtcccctc gtaatcgctt ctgcggcctc
     6241 ctgtttcgct ttcggcagat tattcggggt gacaagtgtc cgcctgttct ccggtgcgtt
     6301 cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc caggcattga ttcgcggacg
     6361 gtccgcccgg tcgtaatagg gctggagccg ttttccgctc gccagctcca tattcgggat
     6421 gacaaaattc agctcaagac gccccttgtc ctggtgctcc acccacagga tgctgtactg
     6481 atttttttca agaccgggca tcagtacccg ctcaaagctc tccatcaccc tttcacgctc
     6541 tcccggtggc agtgtctgct ctgcaaaaga cagaaccccc gaggtgtatt ttttcgcaaa
     6601 cggcgtggca tcgatgagtt cccgcacctc ttcgggagca ccccgcagaa ctctcgcccc
     6661 ttcccggtta cgctc
//
