LOCUS       NZ_AP027249             6774 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia coli strain JNE120393 plasmid pJNE120393-3, complete
            sequence.
ACCESSION   NZ_AP027249
VERSION     NZ_AP027249.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00566463
            Assembly: GCF_030295585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Complete Genome Sequence of Eight LEE-negative Shiga Toxin-producing
            Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome
            Patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2023) Contact:Akiko Kubomura National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 157.4x
            Sequencing Technology             :: Illumina MiSeq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 13:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,076
            Genes (coding)                    :: 4,886
            CDSs (with protein)               :: 4,886
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 80 of 190
            Pseudo Genes (incomplete)         :: 98 of 190
            Pseudo Genes (internal stop)      :: 50 of 190
            Pseudo Genes (multiple problems)  :: 35 of 190
            Pseudo Genes (short protein)      :: 2 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027249.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNE120393"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJNE120393-3"
                     /geo_loc_name="Japan"
                     /collection_date="2012"
                     /note="National Institute of Infectious Diseases JNE120393"
     gene            join(5489..6774,1..934)
                     /gene="mobQ"
                     /locus_tag="QUF33_RS25930"
     CDS             join(5489..6774,1..934)
                     /gene="mobQ"
                     /locus_tag="QUF33_RS25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_100034998.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKATCAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQDHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSNDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLMVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            1167..2837
                     /locus_tag="QUF33_RS25935"
                     /old_locus_tag="JNE120393_49740"
     CDS             1167..2837
                     /locus_tag="QUF33_RS25935"
                     /old_locus_tag="JNE120393_49740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_000012962.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDELAKNKGKIP
                     GLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGRNDPATVYGS
                     PGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2827..3156
                     /locus_tag="QUF33_RS25940"
                     /old_locus_tag="JNE120393_49750"
     CDS             2827..3156
                     /locus_tag="QUF33_RS25940"
                     /old_locus_tag="JNE120393_49750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="WP_000182536.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin transporter"
     gene            3254..3397
                     /locus_tag="QUF33_RS25945"
     CDS             3254..3397
                     /locus_tag="QUF33_RS25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724569.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            3926..4819
                     /locus_tag="QUF33_RS25950"
                     /old_locus_tag="JNE120393_49760"
     CDS             3926..4819
                     /locus_tag="QUF33_RS25950"
                     /old_locus_tag="JNE120393_49760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(5035..5310)
                     /locus_tag="QUF33_RS25955"
                     /old_locus_tag="JNE120393_49770"
     CDS             complement(5035..5310)
                     /locus_tag="QUF33_RS25955"
                     /old_locus_tag="JNE120393_49770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..6774
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5315..5488)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(5489..5847)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3927..4819
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.163"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          21..931
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5490..6718
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.756"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 acaggatcat gaggcgcggc tgaaacgtca tgataaccac cgtctgagcc gtgaaacggc
       61 attagtcggg gttattacgg agctggggcg tgccagagag ccgggaacgg gcaggataac
      121 ccgctacatg atgttgagta acagagccgg agaattcacg gtatggggtg atgagctggc
      181 gcattacccc cagagtgttc atgacccggt gaatgtttac ctgtcgccag gcggggctgt
      241 gatggtctcg gatatacgcg agggaatgcc agaatctcat gagacgatgg cgcggcctga
      301 gcgtgtgaga atgtattccg gtgcgacggt ccggcatgta ctggaacaga tgcgccaggg
      361 gtggccctct tacggttttc cggcgctgcc gcatcactgg ccggataatt tttatttcag
      421 caacgaccgc aggcccgtag cctctccgct accgtcagcg caccgggtgg atatcaccgc
      481 tgatgctgta ccggaacaac tcatgcctgt tgtattttcg agagagggaa acagcaggac
      541 gctgaatctg ttgttgtgca aagggccgga ggaaatgctt gtcgggtttg ttcgccggga
      601 aggagggttt tgccccgttc ttgtgcttcc gtcgccggat tacagtcatc tgatggtcag
      661 caccatcacg gagaacgggg tatgcctggc aggttacgga gaagctataa accatgatga
      721 ggatactccg tacccaccgg aaccgcacct gatgcagttc cggctcaaag gccatcatga
      781 caggcttttg gctgctgtcc acaaaccgga agagatgccg gattatctct tccgtcaact
      841 cggttttaat cagacctggc atgagtggaa gcgggacgaa cagcacaggc aacaacaacg
      901 ccgccccggg cacttccggg gcatgagtat gtgatatccg gggctgcacc ccggaccccg
      961 ccaacacatc acgggccaca aaattttttg tggcccgctc tgcgttttct aagtgttatc
     1021 cctcctgatt tctaaaaaat tttccacctg aacttgacag aaaaaacgat gacgggtact
     1081 ttttgatctg tacataaaac cagtggtttt atgtacagta ttaatcatgt aattaattgt
     1141 tttaacgctt aaaagaggga gtttttatga gcggtggcga tggacgcggc cataacacgg
     1201 gcgcgcatag cacaagtggt aacattaatg gtggcccgac cgggcttggt gtaggtggtg
     1261 gtgcttctga tggctccgga tggagttcgg aaaataaccc gtggggtggt ggttccggta
     1321 gcggcattca ctggggtggt ggttccggtc atggtaatgg cggggggaat ggtaattccg
     1381 gtggtggctc gggaacaggc ggtaatctgt cagcagtagc tgcgccagtg gcatttggtt
     1441 ttccggcact ttccactcca ggagctggcg gtctggcggt cagtatttca gcgggagcat
     1501 tatcggcagc tattgctgat attatggctg ccctgaaagg accgtttaaa tttggtcttt
     1561 ggggggtggc tttatatggt gtattgccat cacaaatagc gaaagatgac cccaatatga
     1621 tgtcaaagat tgtgacgtca ttacccgcag atgatattac tgaatcacct gtcagttcat
     1681 tacctctcga taaggcaaca gtaaacgtaa atgttcgtgt tgttgatgat gtaaaagacg
     1741 aacgacagaa tatttcggtt gtttcaggtg ttccgatgag tgttccggtg gttgatgcaa
     1801 aacctaccga acgtccaggt gtttttacgg catcaattcc aggtgcacct gttctgaata
     1861 tttcagttaa taacagtacg ccagcagtac agacattaag cccaggtgtt acaaataata
     1921 ctgataagga tgttcgcccg gcaggattta ctcagggggg taataccagg gatgcagtta
     1981 ttcgattccc gaaggacagc ggtcataatg ccgtatatgt ttcagtgagt gatgttctta
     2041 gccctgacca ggtaaaacaa cgtcaggatg aagaaaatcg ccgtcagcag gaatgggatg
     2101 ctacgcatcc ggttgaagcg gctgagcgaa attatgaacg cgcgcgtgca gagctgaatc
     2161 aggcaaatga agatgttgcc agaaatcagg agcgacaggc taaagctgtt caggtttata
     2221 attcgcgtaa aagcgaactt gatgcagcga ataaaactct tgctgatgca atagctgaaa
     2281 taaaacaatt tgaacgattt gcacatgacc caatggctgg cggtcacaga atgtggcaaa
     2341 tggccgggct taaagctcag cgggcgcaga cggatgtaaa taataagcag gctgcatttg
     2401 atgctgctgc aaaagagaag gcagatgctg atgctgcatt aagtactgcg atggaaagca
     2461 ggaagaagaa agaggataat aaaagagatg ctgaaggcaa actaaatgat gagttagcaa
     2521 aaaataaagg aaagatacca ggtctaaaaa tagatcagaa aattagggga caaatgccag
     2581 aaagaggatg gacagaggat gatatcaaaa ataccgtatc taacggtgca actggtacat
     2641 cttttgataa gcgtagtccg aagaaaacac ctccagacta tttaggtcga aatgatccag
     2701 caacagtgta tggttctcct ggtaaatatg ttgttgtcaa tgatcgtact ggtgaggtta
     2761 ctcagattag tgataagaca gatccgggtt gggtggacga ttcgagaatt caatggggaa
     2821 ataaaaatga ccaataaatt atttgaacat acggtgttat atgatagtgg tgatgccttt
     2881 tttgaattaa aaggaaatgc ttctatgaag ttatcaccaa aagctgcaat agaagtttgt
     2941 aatgaagcag cgaaaaaagg cttatggatt ttgggcattg atggtgggca ttggctgaat
     3001 cctggattca ggatagatag ttcagcatca tggacatatg atatgccgga ggaatacaaa
     3061 tcaaaaatcc ctgaaaataa tagattggct attgaaaata ttaaagatga tattgagaat
     3121 ggatacactg ctttcattat cacgttaaag atgtaaatag tgttatagaa ttttatgttt
     3181 catggatgat ttcaaccttt ggatttcagg tttttatgga tgaatgcctg agtccatata
     3241 tacaaggaac agtatgaaaa aaataacatg gattatttta ttgcttcttg cagccattat
     3301 tcttgctgca tgtcaggcaa actatatccg cgatgttcag ggcgggactg tatccccgtc
     3361 atcctcagct gaactgaccg gattagcaac gcagtaaccc gaaatcctct ttgacaagaa
     3421 caaagcgtgt caggctgatt ctgacgcgct tttttttgaa gtgtcacaaa aattccatgt
     3481 gggagatggg atctaaaatc cccgtgcaga actttccatc cagggggaga aaacttgtcg
     3541 ttttgagccg ttcggtgttc agaacgcacg aaaccgatcg tgcatcgctt tcgtgcatag
     3601 ttatgcaacg cgctaaaaac gattctgacg cattttttat gtttaccctg gtgtttcctt
     3661 gtctttttgt tttttttgcg tcagaaagcg attgagggtg ttttaagggg tacgtacaac
     3721 gggagttatg gtaaatggat cgggttttcg ggaaggatcg acaggatttg ccgttgggtg
     3781 tagtgtaagc gactgaaaaa caaacgcccc gtaaatcgtg ctctcaccgc caagattgat
     3841 cacgaaatta cagggcgccg ggttccgcgt ttcccgatgg gaaagcgcgg ttagttaaac
     3901 tgtgtaccga gagaaatcgt atcacatgag cgccgtactt caacgcttca gggaaaaatt
     3961 accgcacaaa ccgtactgta cgaacgattt cgcgtacggc gttcgcattc tgccgaaaaa
     4021 cattgccatt cttgcccgtt tcatccagca gaaccagcca catgcactgt actggcttcc
     4081 ctttgacgtg gaccggacgg gggcatcaat cgactggagc gaccggaatt gtccggcccc
     4141 gaacatcacc gtaaaaaatc cccgtaacgg gcacgcgcat ctgctctacg cgctcgccct
     4201 tcctgtgaga actgcgccgg atgcatcggc ttcggcgctc agatacgctg ccgctattga
     4261 gcgtgcgttg tgtgaaaaac tgggcgcgga tgtgaattac agcggcctga tctgcaaaaa
     4321 tccgtgccac cctgaatggc aggaagtgga atggcgcgag gaaccctaca ctctcgacga
     4381 actggctgat tatctcgatt tgagcgcctc agcgcgccgt agcgtcgata aaaattacgg
     4441 gctggggcga aactaccatc tgttcgaaaa ggtccgtaaa tgggcctaca gagcgattcg
     4501 tcagggctgg cctgtattct cacaatggct tgatgccgtg atccagcgtg tcgaaatgta
     4561 caacgcatcg cttcccgttc cgctttctcc ggctgaatgt cgggctattg gcaagagcat
     4621 tgcgaaatat acacacagga aattctcacc tgagggattt tccgctgtac aggccgctcg
     4681 aggtcgcaag ggcggaacta aatctaagcg cgcagcagtt cctacatcag cacgttcgct
     4741 gaagccgtgg gaggcattag ggatcagtcg agcgacgtac taccgaaaat taaaatgtga
     4801 tccagatctc gcaaaatgag accagataag ccttatcaga taacagcgcc cttttggcgt
     4861 ctttttgagc agtgatcttt cttgcgattt tctccgttct gccccgtaat ggcgtggctt
     4921 tccgcatgat tgaggtggta gcgctcgccg cagtctcatg accgagcgta gcgagcgaat
     4981 gagcgaggaa gcgcaaaggc gtccggtggt gcatgggcac ttacgcgccg gggcttagtg
     5041 gttctgcggt ttcgcgggtg gtctgggtag catctccagc tcgttaatca gcggttgtag
     5101 tcggttcaca tccgcctgtc ttgtgacttc ctttcgcaga aactggagca ggaacgcacg
     5161 cagttgcgct tcttccggcc tccgtaccct cgccagcatg gcagccccca caatgacttt
     5221 ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg cctcagcttc
     5281 ggcaatcttc tgttcgagtg ttctgctcat ttcgtgactc cgtgcgcggt gaaaaatcgc
     5341 attttagcgc gtcactggta gttgcaaaac tgaactggca taatgcacgg cacatcacga
     5401 agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg acgctaaaag
     5461 aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca tgaaaatcat
     5521 ttcgcgaaaa aacggctaca gtgctgttgc ttctgctgcc taccgttccg gctctgtcat
     5581 acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg ttgatgatgc
     5641 ggtcattctc acccctgcga atgcaccgtc ctggtgtggt gaccgttccg ttctctggaa
     5701 tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga ttgaacttgc
     5761 cattccccgt gagatttccc gcgaggccgc acgggagacc gttctcgctt ttgtccggga
     5821 aaactttgtc agtcggggca tgattgccga tgtggcgttc catcacatgg accggaccaa
     5881 tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacgggat tcgcaggaaa
     5941 ggtcagggac tggaacgacc gggcactcgc cgagacgtgg cgcgcatcat gggctgacca
     6001 tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc gttcatacga
     6061 gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctacct gtgcgatgga
     6121 aaaacgggga atggaaacag aacgcggtga gcagaaccgt ctgattaaca gccttaacct
     6181 ggaaatacag gtttcccgca cgcagcttgc tctcaggacg gttcaggaag cgcagcgtaa
     6241 gcgggaactc agcgatgctg cacgtcgtgc agcggaagcc cttaacctga ccattcccgc
     6301 tgcgaatgcc tcagcggata ccctgcggga attcattgcc acgctgccgc aggaatgcgg
     6361 gaacgcgtgg gagatgaccc cggagttcct ggcgatgagc gggaaggtga acgacatcga
     6421 acgtgagggg aatgcgctgc tgaaagagca ggccattctc gaaaaggaga tgaccggact
     6481 caaaaaagca cgccctgtcg cgtcccttct gtcagagatt cccctgatga catgggctga
     6541 accggaatac cgcaaaagac aactccgttt ctggaaactc gggaaacaga ttgaatctct
     6601 tcgccgcacc tacagggccg tgaaagaacg ggacattccc gcccgtcgtc aggcctttga
     6661 aacgcagtgg aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa aactgtcagc
     6721 acgggaagcg gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga agga
//
