LOCUS       NZ_AP027248             7769 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia coli strain JNE120393 plasmid pJNE120393-2, complete
            sequence.
ACCESSION   NZ_AP027248
VERSION     NZ_AP027248.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00566463
            Assembly: GCF_030295585.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Complete Genome Sequence of Eight LEE-negative Shiga Toxin-producing
            Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome
            Patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2023) Contact:Akiko Kubomura National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 157.4x
            Sequencing Technology             :: Illumina MiSeq; PacBio RS II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 13:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,076
            Genes (coding)                    :: 4,886
            CDSs (with protein)               :: 4,886
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 80 of 190
            Pseudo Genes (incomplete)         :: 98 of 190
            Pseudo Genes (internal stop)      :: 50 of 190
            Pseudo Genes (multiple problems)  :: 35 of 190
            Pseudo Genes (short protein)      :: 2 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027248.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNE120393"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJNE120393-2"
                     /geo_loc_name="Japan"
                     /collection_date="2012"
                     /note="National Institute of Infectious Diseases JNE120393"
     gene            complement(join(7127..7769,1..830))
                     /locus_tag="QUF33_RS25860"
     CDS             complement(join(7127..7769,1..830))
                     /locus_tag="QUF33_RS25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_286339057.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTVQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            1075..1365
                     /locus_tag="QUF33_RS25865"
                     /old_locus_tag="JNE120393_49590"
     CDS             1075..1365
                     /locus_tag="QUF33_RS25865"
                     /old_locus_tag="JNE120393_49590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024200900.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGMNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(1357..1635)
                     /locus_tag="QUF33_RS25870"
                     /old_locus_tag="JNE120393_49600"
     CDS             complement(1357..1635)
                     /locus_tag="QUF33_RS25870"
                     /old_locus_tag="JNE120393_49600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            1757..2179
                     /locus_tag="QUF33_RS25875"
                     /old_locus_tag="JNE120393_49610"
     CDS             1757..2179
                     /locus_tag="QUF33_RS25875"
                     /old_locus_tag="JNE120393_49610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032319274.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2238..2504)
                     /locus_tag="QUF33_RS25880"
                     /old_locus_tag="JNE120393_49620"
     CDS             complement(2238..2504)
                     /locus_tag="QUF33_RS25880"
                     /old_locus_tag="JNE120393_49620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_286339058.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGR
                     LLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2488..2712)
                     /locus_tag="QUF33_RS25885"
                     /old_locus_tag="JNE120393_49630"
     CDS             complement(2488..2712)
                     /locus_tag="QUF33_RS25885"
                     /old_locus_tag="JNE120393_49630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879770.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2866..3078
                     /locus_tag="QUF33_RS25890"
                     /old_locus_tag="JNE120393_49640"
     CDS             2866..3078
                     /locus_tag="QUF33_RS25890"
                     /old_locus_tag="JNE120393_49640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000350688.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLDK
                     ENGLLICRPVQKEINGTWYTVDLSK"
                     /name="hypothetical protein"
     gene            complement(3610..3843)
                     /locus_tag="QUF33_RS25895"
                     /old_locus_tag="JNE120393_49660"
     CDS             complement(3610..3843)
                     /locus_tag="QUF33_RS25895"
                     /old_locus_tag="JNE120393_49660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3850..4410)
                     /locus_tag="QUF33_RS25900"
                     /old_locus_tag="JNE120393_49670"
     CDS             complement(3850..4410)
                     /locus_tag="QUF33_RS25900"
                     /old_locus_tag="JNE120393_49670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001345151.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4442..4879)
                     /locus_tag="QUF33_RS25905"
                     /pseudo=""
     CDS             complement(<4442..4879)
                     /locus_tag="QUF33_RS25905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5088..5411)
                     /locus_tag="QUF33_RS25910"
                     /old_locus_tag="JNE120393_49680"
     CDS             complement(5088..5411)
                     /locus_tag="QUF33_RS25910"
                     /old_locus_tag="JNE120393_49680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5479..5670
                     /locus_tag="QUF33_RS25915"
                     /old_locus_tag="JNE120393_49690"
     CDS             5479..5670
                     /locus_tag="QUF33_RS25915"
                     /old_locus_tag="JNE120393_49690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6679..6810)
                     /locus_tag="QUF33_RS25920"
     CDS             complement(6679..6810)
                     /locus_tag="QUF33_RS25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            6840..7130
                     /gene="cki"
                     /locus_tag="QUF33_RS25925"
     CDS             6840..7130
                     /gene="cki"
                     /locus_tag="QUF33_RS25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     Typing          complement(5756..5843)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          5875..6413
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3504..5098)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5471..5673)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.88e-12"
ORIGIN
        1 gtcactgacg aatattcatt cttcttatcc tgcaattgtc tctccagttt tttctgaaca
       61 tcacgccatc caggaaatcc agtaaccttc acatcaacac ttttttgagc gttttcaagc
      121 tcccctgcaa ctctggaaac aatcgcttgc ttactctgta catcgctttc ggccttcgcc
      181 agttcagctt tagcctcagc cagcttcttc tctgccactt ccacagcccg atgctcttca
      241 tcaagagaat cctgtcgatt tttctcagcc tctgctgcct ccgccattgc taacaaatgc
      301 gcggtttcag cctgttggcg ggcaatctca tcacgttggc gttctgcttc ccggagcgct
      361 ttttctgctg cttcagcttc ttcacgggct ttttgctctg ccttcgcaag gcgcaaacgc
      421 tcagcctctg cctgcattgc catattattg gcatgagcaa ggtcagttac tgatggacta
      481 cgggcggcat tagcacgtaa agcgtcatta acaatatcct tgagacgttg agtcagggca
      541 tcacgctgag atttcgcttt tgccaatgct gcctcagccg cagcagcacg ggcagcctgt
      601 tcagcctgat gttttttcaa ctgcacggta gaccatttag cggttgcatg tatcgcagca
      661 gaactttcac tggccccacc aaatgctcca ccgcctccac caacattttc agttccagtt
      721 gcaagcgtcg ctgtaatcgt atcgccagta tcaaccccaa cagttgggaa tgacccctca
      781 gtagattctg tgctctccac atctggagaa ttatcagtga ctttatccat aaagaaattc
      841 ctctttgata attaaaacaa taaattaaaa acaatatact gtacatataa ccactggttt
      901 tatgtacagt gaaaacctac tactcagcat tgtccatgtc aagagcatgg attttcattt
      961 ttgcaataag gatcacacta tggggaggca ggcattgaga acgtcgaaac agaacaccgg
     1021 agcaaatcag gatgagatat aaaactgttg gatcatgaaa aaacggagaa cgatgtgagc
     1081 aaatcacccc gccataaact gaacaaaaca gacaaacgac ttctcgacac ccttgttgct
     1141 gccggatatg agcatgacaa agcccgtgac ctcatccaga aacaggttta cacgctgaca
     1201 ctggcagatc agcgtcatgt ggtcagtgaa atcagtaatg gtatgaatcc cacccaggct
     1261 tactcggcgg tataccaggc aagacgcatt cgcctcgccc gtaaatatct gaacggtaaa
     1321 aaggttatgg aagaaaccgg ggaaaatacg cccccatcag cgtaaggatt tcttttgccg
     1381 ctccagagac tccagttttt tacgcaaatc ctctcttttt tgggcatctc tggtgccagc
     1441 cagctctgct ctcaactcat cgatctgaag ttgtatcttc agtctgttac tgaacatttt
     1501 ctgtctggca ttaacatccg caacaatgcc gttttttgtc ttctcggcct tttgttgaaa
     1561 aacattgctg tccgcatgac tggcaaccga agcagaaaga acactaaaaa gcaggactgg
     1621 cacacatttt ttcacgggat tattcctgac tcattgacca tcaaatcaca ttgggagtaa
     1681 accgacgtat gataagagat actcttcgga gatataactc cctgagtatc aagattaaaa
     1741 acgcaaggag atgtttatga gatctgccgc tgccaggctg cttctgatac ctctgataac
     1801 agcaacaata gctcttacag gatgcacacc aaagaccagc ctggaacgac atacccggca
     1861 ttatgtttat gcttcagatg atggatttga tcctaacttc tacacccaga aagcagacac
     1921 catacgtatg atgctcccgt tctttcagca gttccgggat atggggatga aagacaaagc
     1981 agccggagta tcagcagaaa cggcacagca acgtgtaaaa gaattccact cagaaaaatt
     2041 ttttcactca ctccagagca caacaacctt tgctggcaga aaatacacaa acagcgatat
     2101 gccttcgccg aaaaaaatga aactaatggc agacaccatt tctgcggttt atctcgatgg
     2161 atacgagggc agacagtaag ggatttatca taatccctta ttgtacgcca catatgaaaa
     2221 agcccgcatg gcgggcttca gtcgtagact tcacgtcgat ggccgatggt ggccacaaga
     2281 ataatcagtt cgtcatccct gatttcacac agaatgcgat aatcacccac acgataacgc
     2341 cagaattctc ccaactcccc cttcagtagc ctccctgatt tacggggatc ggctgcaact
     2401 gcaatacgta aatccataaa atccacaatc cgtcgcgcgt tctgcttgtc catcttacgt
     2461 agcgatttga gcgcccgatc ggaataatta atcgtccaga ccaagagact tccttacgtc
     2521 ctcagacgaa tgcacagcct catcaccacg gcgaacacgt gctgctgttt ccgctgaaag
     2581 ataataatct tccagatctt ccaggtgcgc cagtatcgct tcccgggcat agaacgtctt
     2641 tgtcctcccg gttttcttcg ccagcgagtc cagacgggac tcaatttcat cagacaaccg
     2701 aatagccagc atacaaatct ccctccgaac aattgttata catgtatagc aaaaattata
     2761 acgtaaaaag acgataaaac aatcaaaggc tggttgaata aacagtaaaa tcccatatat
     2821 taacgactga tatatgataa gtatcggttt taaaaggaaa acaacatgga tattgaaatg
     2881 aataacactg aagagatgtt cagcaaactg gggatgcggt taggagctga gatggcaatc
     2941 cctttgaagg atggtgataa ttttgcgcca actggatgca tcgttacgaa gcttgataaa
     3001 gagaatggcc ttctgatctg ccgaccagtt cagaaggaaa tcaacggcac gtggtacacc
     3061 gtcgatttaa gtaagtaaca ccacggacta aagccctgcg gggctttttt atacctgccg
     3121 ctgactctga ttgaacataa catctgaatt gtacgcaccg ctgaaatgcg ttcagcgcga
     3181 tcacggctgc tgacaggtaa aaatggcaac aaaccacccg aaaagctgcc gcgatcgcac
     3241 ctgataaatt ttaaccgtat gcatagctat tcagccatgt gaataacgct ggttttgcct
     3301 gcgtaaacct catgacactg ttttttttcc atcttttcag ttgatgacat acgcagacat
     3361 cgcgggatga ggctgaggaa tgagcgcgat ctggcaaaga ggcaaaacac agcaacaaaa
     3421 acgacacgcc agaatcgcgc ccggatgcgt ttttaacgcg ttccggtacc atctggcaac
     3481 ctcccggaac aactcaccgt cacatcccca ttgacgggcc acgccatacc cgtgcttccc
     3541 gttcctgctc ttcatgccag gaccgcgcac gctcccgttc caggcgtgcc tgcctttcct
     3601 gttcatccct tatctgctgt tcgtgataaa taaccgactc aagcggtcca cctgcccggc
     3661 taatctctgc acctgctgac tcaagtcgtc gcactgttcc ctcagttgcc cgttctcctg
     3721 tcgtgtcagc tcgaacatat gctgcaaatc cgtgaaggcg ctctcccagt ctttcagccg
     3781 ctgcgtatag tcctgctgca attgctctaa ggcgttcagt aagtgcattt ccagctctgt
     3841 catactcact tactccctga ccagtcttac tgcgttcttc ttctccaccg tccagttgtt
     3901 ttcccccttc accccggacg gcaacaccag aaatttcccg ttcctgccct cgtgatacgt
     3961 cacaccccat gttttttccc ggagtttcgc cagcgtctct tcctggtccc tgatagccag
     4021 gatgttcgcc gcaatacggc tttcctgcca ctgaatcagc ccccatgacg ccagaaaaat
     4081 cccgaccgtg acgcaaagcg ccgtcagcga cgggtacagt atccgccctt tgaccagctt
     4141 ccagagcagc tcttcctgcc gccgggcaag ttcgttctct gtggcgctga actgcgcgtt
     4201 cacggcactg ttcagcgtct ccagttgttc tttcaccgct gctgtgtgtg cgctgatagc
     4261 gtctctgatt ttctgcccgt ttaagttcag ttccctgtct acagacgctt cgagcttcct
     4321 gaactcgctg ttcagcatgt tctctgtaga gacggcacgc tctttcagtt tcttctcgaa
     4381 gtctgtcccc atttgtaaaa gattgctcat acagcgcccc tttcagcctg agattacgcc
     4441 cgccctccgg gtcggcgata ctgatactgc tcctggttgt cctcaccacc tcaaaacctg
     4501 ccgctgtaag cgcctcagtg acatcctgac gcgtttttag cgctccggca tggtaaagag
     4561 cctccagtcc cctcgtaatc gcttctgcgg cctcctgttt cgctttcggc agattattcg
     4621 gggtgacaag tgtccgcctg ttctccggtg cgttcgggtc gtgcagcccg taatggtgat
     4681 tcaccagtgt ctgccaggca ttgattcgcg gacggtccgc ccggtcgtaa tagggctgga
     4741 gccgttttcc gctcgccagc tccatattcg ggatgacaaa attcagctca agacgcccct
     4801 tgtcctggtg ctccacccac aggatgctgt actgattttt ttcaagaccg ggcatcagta
     4861 cccgctcaaa gctctccatc accctttcac gctctcccgg tggcagtgtc tgctctgcaa
     4921 aagacagaac ccccgaggtg tattttttcg caaacggcgt ggcatcgatg agttcccgca
     4981 cctcttcggg agcaccccgc agaactctcg ccccttcccg gttacgctcc cggcccagca
     5041 ggtaatcaac cggaccactg ccaccgcctt ttcccctggc atgaaactta actatcatcg
     5101 cgttctccct gtttacggac ctcatccctc agctcactca gttcacgtcc gatggccatc
     5161 agtgcagcca ccacatgaac ccggtcatgc cccgaccact gtccactgtt tatcttccgg
     5221 gctatctgat tcaggttatt gccgaccgaa gcgaactggc gcaacagcgg cggtgccagt
     5281 gtcggaagac ctgacgtttt cgatggcggt gcccccaggc agaccttacg catccatgac
     5341 gcaagttgtt ttccctcaca acgtgccagc agccgcgcat gttcctcatc cgtgacccgt
     5401 atcgtgagca tcctttcgcg tttcaccggt atcattaaaa acctccgaca gactccccac
     5461 acatggagaa acagaactgt gactaaacag gaaaaaaccg cccttaacat ggcccgcttc
     5521 atcagaagcc agacgctgac cctgctggaa aaactgaatg aactggacgc cgacgaccag
     5581 gctgacatct gcgaagcgct tcacgaccac gctgacgagc tttaccgcag ctgcctcgca
     5641 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     5701 tgcctgtgag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggttttagc
     5761 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     5821 attatgcggc aacagtgcgg attgtatgaa aagtgcgcca tgccgggtgt gaaatgccgc
     5881 acagatgcgt aaggagagaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     5941 cgctccgctc ggtcgttcgg ctgcggcgag cggactctcc tttctcaaag gcggtaaccc
     6001 cgttatccac agaatccggg gataacccgg gaaagaacat gtgcgcaaaa aaacgaagac
     6061 cccgggaaag gccgcggcac aggcgctttt tccataggct ccgcccccct gacgagcatc
     6121 acaaaaaccg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc
     6181 gtttcccccc ggaagctccc tcgtgcgctc tcctgttccg accctgccga ttaccggata
     6241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     6301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6421 cttaacgcca ctggcagcag ccattggtaa ctggatagtg gatttagata cgcagaactc
     6481 ttgaagttga agccttatag cggctacact ggaaggacag catttggtat ctgtgctcca
     6541 ctaaagccag ttacccggtt aagcagtccc caactgactt aaccttcgac taaaccgcct
     6601 ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
     6661 aagatccttt gatcttttct actgaaccgc gatccccgtc agttcagaag acgaagatgg
     6721 tgcaacggtt cctccttgca catcacgaat atagtttgct tgacatcctg acaagaataa
     6781 tattgtaatt agaaaaatta ttattttcat atttctttcc aacaaaagta aaaatactta
     6841 tgcatttaaa atactaccta cataatttac ctgaatcact tataccatgg attcttattt
     6901 taatatttaa cgacaatgat aacactcctt tgttatttat atttatatca tcaatacatg
     6961 tattgctata tccatactct aaattaacca tatctagata tatcaaagaa aatacaaagt
     7021 taaaaaaaga accctggtac ttatgcaagt tatctgcatt gttttattta ttaatggcaa
     7081 tcccagtagg attgccaagt ttcatatatt acactctaaa gagaaattaa atccctaaca
     7141 actcattaag tttgttcaac tcatcatccc ctatgtaaga agaaacaata cctgtgacaa
     7201 ttgcaatccc ccagaaacca agaggcacac caacaataaa actaaacatt aatgccacaa
     7261 tttttgccac acctacatcg accgcacttt tctccagcgt gacaaacaaa ggtcgccagt
     7321 taccagtttc tattgcattt ttaaaatcag aaccaacatc atatagaaaa gatactcgac
     7381 tagtgatttt aagcgacttt gatatcttcg ttaaattctt agataactca tcataattaa
     7441 cagactctaa cgcattaaaa atagcatccc gatcaacctt actgattttc ttatccagta
     7501 catttttata cttttcaaat gcagcaagag cttcatcaac actactaaat ttactaccct
     7561 tcgccttttc agctaaatcc tgagctattc tggaatattt ttccccatat tgttcggtta
     7621 tatattgata aaacccaacc atagtatcaa cagagtcttt tacctgactc ttctcaagag
     7681 catccttagc ttcttttaat tttatctcag cattctgaac atctgttttt ttagcatctc
     7741 taatgctaac cgcagaatta agagcattc
//
