LOCUS       NZ_AP027215             6745 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia coli strain JNE131328 plasmid pJNE131328-3, complete
            sequence.
ACCESSION   NZ_AP027215
VERSION     NZ_AP027215.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00566111
            Assembly: GCF_030295525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Complete Genome Sequence of Eight LEE-negative Shiga Toxin-producing
            Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome
            Patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Akiko Kubomura National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.5.0; Trycycler v. 0.5.3
            Genome Coverage                   :: 162.5x
            Sequencing Technology :: Illumina MiSeq; PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 12:23:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,131
            CDSs (total)                      :: 5,003
            Genes (coding)                    :: 4,820
            CDSs (with protein)               :: 4,820
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 79 of 183
            Pseudo Genes (incomplete)         :: 99 of 183
            Pseudo Genes (internal stop)      :: 43 of 183
            Pseudo Genes (multiple problems)  :: 35 of 183
            Pseudo Genes (short protein)      :: 2 of 183
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027215.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNE131328"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJNE131328-3"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
                     /note="National Institute of Infectious Diseases JNE131328"
     gene            1..915
                     /locus_tag="QUE42_RS25625"
                     /old_locus_tag="JNE131328_p30010"
     CDS             1..915
                     /locus_tag="QUE42_RS25625"
                     /old_locus_tag="JNE131328_p30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_286334606.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1133..1408)
                     /locus_tag="QUE42_RS25630"
                     /old_locus_tag="JNE131328_p30020"
     CDS             complement(1133..1408)
                     /locus_tag="QUE42_RS25630"
                     /old_locus_tag="JNE131328_p30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091751.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
                     /name="hypothetical protein"
     gene            1587..3806
                     /gene="mobQ"
                     /locus_tag="QUE42_RS25635"
                     /old_locus_tag="JNE131328_p30030"
     CDS             1587..3806
                     /gene="mobQ"
                     /locus_tag="QUE42_RS25635"
                     /old_locus_tag="JNE131328_p30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_024224537.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4039..5784
                     /gene="col"
                     /locus_tag="QUE42_RS25640"
                     /old_locus_tag="JNE131328_p30040"
     CDS             4039..5784
                     /gene="col"
                     /locus_tag="QUE42_RS25640"
                     /old_locus_tag="JNE131328_p30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_286334605.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHID
                     IHRGK"
                     /name="col"
     gene            5787..6047
                     /locus_tag="QUE42_RS25645"
                     /old_locus_tag="JNE131328_p30050"
     CDS             5787..6047
                     /locus_tag="QUE42_RS25645"
                     /old_locus_tag="JNE131328_p30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6109..6252
                     /locus_tag="QUE42_RS25650"
                     /old_locus_tag="JNE131328_p30060"
     CDS             6109..6252
                     /locus_tag="QUE42_RS25650"
                     /old_locus_tag="JNE131328_p30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6745
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1413..1586)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(1587..1945)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..915
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.204"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1588..3803
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.917"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgtaaa aaatcccgtg
      241 aacggtcatg ctcacctgct ctatgcactc aacatcgccg tgagaaccgc gcctgatgcg
      301 tcggttaagg cactgaagta cgctgccgcg atagagcgtg cgctgtgtga gaaacttggt
      361 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg tatcgcttcc cgttccgctt
      661 tctccggcgg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgtggtcgca agggcggttc taagtctaag cgcggcacag tagctacatc agcacgcacg
      841 ctgaaacctt gggagaaatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtcttgtag agtagaccaa ataaaaccta tatcagataa cagcgccttt ttggcgcttt
      961 tttgagcagc ttggtttgtt gctatttccc tcgttgaatc ccgcaatggc gcggctttcc
     1021 gcatgattga ggttgtagcg ctcgccgcag tctcatgacc gagcgtagcg agcgaatgag
     1081 cgaggaagcg caaaggcgtc cggtggtgca tgtggctctt acgcgccggg gcttagtggt
     1141 tctgcggttt cgcaggtggt ttgggtagct tctccagctc gttaatcagc ggttgtagtc
     1201 ggttcacatc cgcctgtctt gtgacttcct ttcgcagaaa ctggagcagg aacgcacgca
     1261 gttgcgcctc ttccggcctc cgtaccctcg ccagcatggc agcccccaca atgacttttt
     1321 gcgccgtgtc caggctccgg ctcttcgcct tcaggcgctg taatctggcc tcagcttcgg
     1381 caatcttctg ttcgagtgtt ctgctcattt tgtgactccg tgcgcggtga aaaatcgcat
     1441 tttatcgcgt cactggtagt tgcaaaactg aactggcata atgcacggca catcacgaag
     1501 tgcgcactta tacaatctcc acttcgtttc gattgtgtgc ggtctgcgac gctaaaagaa
     1561 aacggcaaaa aggcattacg gcagaaatgg cgatttatca tctcagcatg aaaatcattt
     1621 cgcgaaaaaa cggctacagt gctgttgctt ctgctgccta ccgttccggc tctgtcatac
     1681 ccgatgaccg taccggatta atccacgatt acacccgtaa acgcggcgtt gatgatgcgg
     1741 tcattctcac ccctgcgaat gcaccgtcct ggtgtggtga ccgttccgtt ctctggaatg
     1801 cggtcgagaa agccgaacag cgccggaact cccagctggc aagggagatt gaactcgcca
     1861 ttccccgtga gatttcccgc gaggccgcac gggagaccgt tctcgctttt gtccgggaaa
     1921 actttgtcag tcggggcatg attgccgatg tggcgttcca tcacatggac cggaccaatc
     1981 cccatgcgca catcatgctg accacgagag ctgtcgggga aacgggattc gcaggaaagg
     2041 tcagggactg gaacgaccgg gcactcgccg agacgtggcg cgcatcatgg gctgaccatg
     2101 cgaacagagc gcttgcgaac gccggctacc aggaagagat agaccatcgt tcatacgagc
     2161 gtcagggact ggagaaagcg ccgggccttc acctcggaaa ggctgcctgt gcgatggaaa
     2221 aacggggaat ggaaacagaa cgcggtgagc agaaccgcct gattaacagc cttaacctgg
     2281 aaatacaggt ttcccgcaca aggctggcag tcatccaggt acaggagaac gtgcggaaac
     2341 gggaactcag cgatgccgca cgtcgtgcag cggaagccct taacctgacc attcccgctg
     2401 cgaatgcctc agcggatacc ctgcgggaat tcattgccac gttgccgcag gaatgcggca
     2461 acgcgtggga gatgaccccg gagttcctgg cgatgagcgg gaaggtgaac gacatcgaac
     2521 gtgaggggaa tgcgctgctg aaagagcagg ccattctcga aaaggagatg accggactca
     2581 aaaaagcacg ccctgtcgcg tcccttctgt cagagattcc cctgatgaca tgggctgaac
     2641 cggaataccg caaaagacaa ctccgtttct ggaaactcgg gaaacagatt gaatctcttc
     2701 gccgcaccta cagggccgtg aaagaacagg acattcccgc ccgtcgtcag gcctttgaaa
     2761 cgcagtggaa tacgtggatt gcgccgggaa tggcagagct gaaagaaaaa ctgtcagcac
     2821 gggaagcgga gcggcgcagg gaggaagccg aagcggaagc gcgccggaag gaacaggagc
     2881 atgaggcgcg gctgaaacgt catgataacc accgtctgag ccgtgaaacg gcattagtcg
     2941 gggttattac ggagctgggg cgtgccagag agccgggaac gggcaggata acccgctaca
     3001 tgatgctgag taacagagcc ggagaattca cggtatgggg tgatgagctg gcgcattacc
     3061 cccagagtgt tcatgacccg gtgaatgttt acctgtcgct aggcggggct gtgatggtct
     3121 cggatatacg tgagggaatg ccagaatctc atgagacgat ggcgcggcct gagcgtgtga
     3181 gaatgtattc cggtgcgacg gtccggcatg tactggaaca gatgcgccag gggtggccct
     3241 cttacggttt tccggcgctg ccgcatcact ggccggataa tttttatttc agcgacgacc
     3301 gcaggcccgt agcctctccg ctgccgtcag cgcaccgggt ggatatcacc gctgatgctg
     3361 taccggaaca actcatgcct gttgtatttt cgagagaggg aaacagcagg acgctgaatc
     3421 tgttgttatg caaagggccg gaggaaatgc ttgtcgggtt tgttcgccgg gaaggagggt
     3481 tttgccccgt tcttgtgctt ccgtcgccgg attacagtca tctgatagtc agcaccatca
     3541 cggagaacgg ggtatgcctg gcaggttacg gagaagctat aaaccatgat gaggatactc
     3601 cgtacccacc ggaaccgcac ctgatgcagt tccggctcaa aggccatcat gacaggcttt
     3661 tggctgctgt ccacaaaccg gaagagatgc cggattatct cttccgtcaa ctcggtttta
     3721 atcagacctg gcatgagtgg aagcgggacg aacagcacag gcaacaacaa cgccgccccg
     3781 ggcacttccg gggcatgagt atgtgatgtc cggggctgca ccccggaccc cgccaacaca
     3841 tcacgggcca caaaattttt tgtggcccgc tctgcgtttt ctaagtgtta tccctcctga
     3901 tttctaaaaa attttccacc tgagcttgac agaaaaaacg atgacgggta ctttttgatc
     3961 tgtacataaa accagtggtt ttatgtacag tattaatcat gtaattaatt gttttaacgc
     4021 ttaaaagagg gaatttttat gagcggtggc gatggacgcg gccataacac gggcgcgcat
     4081 agcacaagtg gtaacattaa tggtggcccg accgggcttg gtgtaggtgg tggtgcttct
     4141 gatggctccg gatggagttc ggaaaataac ccgtggggtg gtggttccgg tagcggcatt
     4201 cactggggtg gtggttccgg tcatggtaat ggcgggggga atggtaattc cggtggtggc
     4261 tcgggaacag gcggtaatct gtcagcagta gctgtgccag tggcatttgg ttttccggca
     4321 ctttccactc caggagctgg cggtctggcg gtcagtattt cagcgggagc attatcggca
     4381 gctattgctg atattatggc tgccctgaaa ggaccgttta aatttggtct ttggggggtg
     4441 gctttatatg gtgtattgcc atcacaaata gcgaaagatg accccaatat gatgtcaaag
     4501 attgtgacgt cattacccgc agatgatatt actgaatcac ctgtcagttc attacctctc
     4561 gataaggcaa cagtaaacgt aaatgttcgt gttgttgatg atgtaaaaga cgaacgacag
     4621 aatatttcgg ttgtttcagg tgttccgatg agtgttccgg tggttgatgc aaaacctacc
     4681 gaacgtccgg gtgtttttac ggcatcaatt ccaggtgcac ctgttctgaa tatttcagtt
     4741 aataacagta cgccagcagt acagacatta agcccaggtg ttacaaataa tactgataag
     4801 gatgttcgcc cggcaggatt tactcagggt ggtaatacca gggatgcagt tattcgattc
     4861 ccgaaggaca gcggtcataa tgccgtatat gtttcagtga gtgatgttct tagccctgac
     4921 caggtaaaac aacgtcagga tgaagaaaat cgccgtcagc aggaatggga tgctacgcat
     4981 ccggttgaag cggctgagcg aaattatgaa cgcgcgcgtg cagagctgaa tcaggcaaat
     5041 gaagatgttg ccagaaatca ggagcgacag gctaaagctg ttcaggttta taattcgcgt
     5101 aaaagcgaac ttgatgcagc gaataagact cttgctgatg caatagctga aataaaacaa
     5161 tttgatcgat ttgcccatga cccaatgtct ggcggtcaca gaatgtggca aatggccggg
     5221 cttaaagccc agcgggcgca gacggatgta aataataagc aggctgcatt tgatgctgct
     5281 gcaaaagaga agtcagatgc tgatgctgca ttaagtgccg cgcaggagcg ccgcaaacag
     5341 aaggaaaata aagaaaagga cgctaaggat aaattagata aggagagtaa acggaataag
     5401 ccagggaagg cgacaggtaa aggtaaacca gttggtgata aatggctgga tgatgcaggt
     5461 aaagattcag gagcgccaat tccagatcgc attgctgata agttgcgtga taaagaattt
     5521 aaaaacttcg acgattttcg gagaaaattc tgggaagaag tgtcgaaaga tcctgagtta
     5581 agtaagcaat ttaaaggcag taataagacg aacattcaaa agggaaaagc accttttgca
     5641 aggaagaaag accaagtagg tggtagggaa cgctttgaat tacatcatga taaaccaatc
     5701 agtcaggatg gtggtgtcta tgatatgaat aatatcagag tgaccacacc taagcgacat
     5761 attgatattc atcggggtaa gtaaaaatgg aactgaaaca tagtattagt gattataccg
     5821 aggctgaatt tctggagttt gtaaaaaaaa tatgtagagc tgaaggtgct actgaagagg
     5881 atgacaataa attagtgaga gagtttgagc gattaactga gcacccagat ggttcagatc
     5941 tgatttatta tcctcgcgat gacagggaag atagtcctga agggattgtc aaagagatta
     6001 aagaatggcg agctgctaac ggtaagtcag gatttaaaca gggctgaaat atgaatgcca
     6061 gttgtttaag gatgaatgac tggcattctt tcacaacaag gagtcgttat gaaaaaaata
     6121 acagggatta ttttattgct tcttgcagtc attattctgg ctgcatgtca ggcaaactat
     6181 atccgggatg ttcagggcgg gaccgtatct ccgtcatcaa cagctgaagt gaccggatta
     6241 gcaacgcagt aacccgaaat cctctttgac aaaaacaaag cgtgtcaggc tgattctgat
     6301 gcgctttttt tgaaatgtca caaaaattcc atgtgggaga tgggatctaa aatcctcgtg
     6361 cagaactttc catccagggg gagaaaactt gtcgttttga gccgttcggt gttcagaacg
     6421 cacgaaaccg atcgcgcatc gctttcgtga atagttatgc aggcccctga aaacgattct
     6481 gacgcgtttt ttcggttttg cctggtgttt tcctgtcttt ttgcgttttt tgcgtcagaa
     6541 cgcgtctgag ggcgttttaa ggggtgcgta caacgggagt tatggtaaat ggatcgggtt
     6601 ttcgggaagg ttcgacagga tttgccgttg ggtgtagtgt aagcgactga aaaacaaacg
     6661 cccctgaaat tcgtgtctct ccgccaagat tgtcacgaga atacaggggc gaggtcggtc
     6721 gataacctgg aaggaatcgt aacac
//
