LOCUS       NZ_AP027214            15094 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia coli strain JNE131328 plasmid pJNE131328-2, complete
            sequence.
ACCESSION   NZ_AP027214
VERSION     NZ_AP027214.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00566111
            Assembly: GCF_030295525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Complete Genome Sequence of Eight LEE-negative Shiga Toxin-producing
            Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome
            Patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15094)
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Akiko Kubomura National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.5.0; Trycycler v. 0.5.3
            Genome Coverage                   :: 162.5x
            Sequencing Technology :: Illumina MiSeq; PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 12:23:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,131
            CDSs (total)                      :: 5,003
            Genes (coding)                    :: 4,820
            CDSs (with protein)               :: 4,820
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 79 of 183
            Pseudo Genes (incomplete)         :: 99 of 183
            Pseudo Genes (internal stop)      :: 43 of 183
            Pseudo Genes (multiple problems)  :: 35 of 183
            Pseudo Genes (short protein)      :: 2 of 183
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027214.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNE131328"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJNE131328-2"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
                     /note="National Institute of Infectious Diseases JNE131328"
     gene            complement(69..281)
                     /locus_tag="QUE42_RS25505"
                     /old_locus_tag="JNE131328_p20010"
     CDS             complement(69..281)
                     /locus_tag="QUE42_RS25505"
                     /old_locus_tag="JNE131328_p20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000350688.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLDK
                     ENGLLICRPVQKEINGTWYTVDLSK"
                     /name="hypothetical protein"
     gene            435..659
                     /locus_tag="QUE42_RS25510"
                     /old_locus_tag="JNE131328_p20020"
     CDS             435..659
                     /locus_tag="QUE42_RS25510"
                     /old_locus_tag="JNE131328_p20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879770.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            643..909
                     /locus_tag="QUE42_RS25515"
                     /old_locus_tag="JNE131328_p20030"
     CDS             643..909
                     /locus_tag="QUE42_RS25515"
                     /old_locus_tag="JNE131328_p20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262325.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGR
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(968..1390)
                     /locus_tag="QUE42_RS25520"
                     /old_locus_tag="JNE131328_p20040"
     CDS             complement(968..1390)
                     /locus_tag="QUE42_RS25520"
                     /old_locus_tag="JNE131328_p20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032319274.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1512..1790
                     /locus_tag="QUE42_RS25525"
                     /old_locus_tag="JNE131328_p20050"
     CDS             1512..1790
                     /locus_tag="QUE42_RS25525"
                     /old_locus_tag="JNE131328_p20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1782..2072)
                     /locus_tag="QUE42_RS25530"
                     /old_locus_tag="JNE131328_p20060"
     CDS             complement(1782..2072)
                     /locus_tag="QUE42_RS25530"
                     /old_locus_tag="JNE131328_p20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024200900.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGMNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            2317..3789
                     /locus_tag="QUE42_RS25535"
                     /old_locus_tag="JNE131328_p20070"
     CDS             2317..3789
                     /locus_tag="QUE42_RS25535"
                     /old_locus_tag="JNE131328_p20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000362089.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(3786..4076)
                     /gene="cki"
                     /locus_tag="QUE42_RS25540"
     CDS             complement(3786..4076)
                     /gene="cki"
                     /locus_tag="QUE42_RS25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            4106..4237
                     /locus_tag="QUE42_RS25545"
     CDS             4106..4237
                     /locus_tag="QUE42_RS25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5246..5437)
                     /locus_tag="QUE42_RS25550"
                     /old_locus_tag="JNE131328_p20090"
     CDS             complement(5246..5437)
                     /locus_tag="QUE42_RS25550"
                     /old_locus_tag="JNE131328_p20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5505..5828
                     /locus_tag="QUE42_RS25555"
                     /old_locus_tag="JNE131328_p20100"
     CDS             5505..5828
                     /locus_tag="QUE42_RS25555"
                     /old_locus_tag="JNE131328_p20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6037..>6474
                     /locus_tag="QUE42_RS25560"
                     /pseudo=""
     CDS             6037..>6474
                     /locus_tag="QUE42_RS25560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6506..7066
                     /locus_tag="QUE42_RS25565"
                     /old_locus_tag="JNE131328_p20110"
     CDS             6506..7066
                     /locus_tag="QUE42_RS25565"
                     /old_locus_tag="JNE131328_p20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            7073..7306
                     /locus_tag="QUE42_RS25570"
                     /old_locus_tag="JNE131328_p20120"
     CDS             7073..7306
                     /locus_tag="QUE42_RS25570"
                     /old_locus_tag="JNE131328_p20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7791..8210)
                     /locus_tag="QUE42_RS25575"
                     /old_locus_tag="JNE131328_p20130"
     CDS             complement(7791..8210)
                     /locus_tag="QUE42_RS25575"
                     /old_locus_tag="JNE131328_p20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(8294..8731)
                     /locus_tag="QUE42_RS25580"
                     /old_locus_tag="JNE131328_p20140"
     CDS             complement(8294..8731)
                     /locus_tag="QUE42_RS25580"
                     /old_locus_tag="JNE131328_p20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177063.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRR
                     EFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            8989..11082
                     /gene="cda"
                     /locus_tag="QUE42_RS25585"
                     /old_locus_tag="JNE131328_p20150"
     CDS             8989..11082
                     /gene="cda"
                     /locus_tag="QUE42_RS25585"
                     /old_locus_tag="JNE131328_p20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001282379.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPAAGN
                     NVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVKQPPVY
                     TQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGMVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDAGGLRDFIYWR
                     PDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            11079..11342
                     /locus_tag="QUE42_RS25590"
                     /old_locus_tag="JNE131328_p20160"
     CDS             11079..11342
                     /locus_tag="QUE42_RS25590"
                     /old_locus_tag="JNE131328_p20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038405.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKDLSQDVL
                     NCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            11412..11558
                     /locus_tag="QUE42_RS25595"
     CDS             11412..11558
                     /locus_tag="QUE42_RS25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071527535.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTGV
                     AIQ"
                     /name="colicin release lysis protein"
     gene            complement(12571..12762)
                     /locus_tag="QUE42_RS25600"
                     /old_locus_tag="JNE131328_p20180"
     CDS             complement(12571..12762)
                     /locus_tag="QUE42_RS25600"
                     /old_locus_tag="JNE131328_p20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            12830..13153
                     /locus_tag="QUE42_RS25605"
                     /old_locus_tag="JNE131328_p20190"
     CDS             12830..13153
                     /locus_tag="QUE42_RS25605"
                     /old_locus_tag="JNE131328_p20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            13362..>13799
                     /locus_tag="QUE42_RS25610"
                     /pseudo=""
     CDS             13362..>13799
                     /locus_tag="QUE42_RS25610"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            13831..14391
                     /locus_tag="QUE42_RS25615"
                     /old_locus_tag="JNE131328_p20200"
     CDS             13831..14391
                     /locus_tag="QUE42_RS25615"
                     /old_locus_tag="JNE131328_p20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            14398..14631
                     /locus_tag="QUE42_RS25620"
                     /old_locus_tag="JNE131328_p20210"
     CDS             14398..14631
                     /locus_tag="QUE42_RS25620"
                     /old_locus_tag="JNE131328_p20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     BGC             3989..15094
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5074..5158
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          12399..12483
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(4504..5042)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11829..12367)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5819..7413
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13144..14738
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagatgttat gttcaatcag agtcagcggc aggtataaaa aagccccgca gggctttagt
       61 ccgtggtgtt acttacttaa atcgacggtg taccacgtgc cgttgatttc cttctgaact
      121 ggtcggcaga tcagaaggcc attctcttta tcaagcttcg taacgatgca tccagttggc
      181 gcaaaattat caccatcctt caaagggatt gccatctcag ctcctaaccg catccccagt
      241 ttgctgaaca tctcttcagt gttattcatt tcaatatcca tgttgttttc cttttaaaac
      301 cgatacttat catatatcag tcgttaatat atgggatttt actgtttatt caaccagcct
      361 ttgattgttt tatcgtcttt ttacgttata atttttgcta tacatgtata acaattgttc
      421 ggagggagat ttgtatgctg gctattcggt tgtctgatga aattgagtcc cgtctggact
      481 cgctggcgaa gaaaaccggg aggacaaaga cgttctatgc ccgggaagcg atactggcgc
      541 acctggaaga tctggaagat tattatcttt cagcggaaac agcagcacgt gttcgccgtg
      601 gtgatgaggc tgtgcattcg tctgaggacg taaggaagtc tcttggtctg gacgattaat
      661 tattccgatc gggcgctcaa atcgctacgt aagatggaca agcagaacgc gcgacggatt
      721 gtggatttta tggatttacg tattgcagtt gcagccgatc cccgtaaatc agggaggcca
      781 ctgaaggggg agttgggaga attctggcgt tatcgtgtgg gtgattatcg cattctgtgt
      841 gaaatcaggg atgacgaact gattattctt gtggccacca tcggccatcg acgtgaagtc
      901 tacgactgaa gcccgccatg cgggcttttt catatgtggc gtacaataag ggattatgat
      961 aaatccctta ctgtctgccc tcgtatccat cgagataaac cgcagaaatg gtgtctgcca
     1021 ttagtttcat ttttttcggc gaaggcatat cgctgtttgt gtattttctg ccagcaaagg
     1081 ttgttgtgct ctggagtgag tgaaaaaatt tttctgagtg gaattctttt acacgttgct
     1141 gtgccgtttc tgctgatact ccggctgctt tgtctttcat ccccatatcc cggaactgct
     1201 gaaagaacgg gagcatcata cgtatggtgt ctgctttctg ggtgtagaag ttaggatcaa
     1261 atccatcatc tgaagcataa acataatgcc gggtatgtcg ttccaggctg gtctttggtg
     1321 tgcatcctgt aagagctatt gttgctgtta tcagaggtat cagaagcagc ctggcagcgg
     1381 cagatctcat aaacatctcc ttgcgttttt aatcttgata ctcagggagt tatatctccg
     1441 aagagtatct cttatcatac gtcggtttac tcccaatgtg atttgatggt caatgagtca
     1501 ggaataatcc cgtgaaaaaa tgtgtgccag tcctgctttt tagtgttctt tctgcttcgg
     1561 ttgccagtca tgcggacagc aatgtttttc aacaaaaggc cgagaagaca aaaaacggca
     1621 ttgttgcgga tgttaatgcc agacagaaaa tgttcagtaa cagactgaag atacaacttc
     1681 agatcgatga gttgagagca gagctggctg gcaccagaga tgcccaaaaa agagaggatt
     1741 tgcgtaaaaa actggagtct ctggagcggc aaaagaaatc cttacgctga tgggggcgta
     1801 ttttccccgg tttcttccat aaccttttta ccgttcagat atttacgggc gaggcgaatg
     1861 cgtcttgcct ggtataccgc cgagtaagcc tgggtgggat tcataccatt actgatttca
     1921 ctgaccacat gacgctgatc tgccagtgtc agcgtgtaaa cctgtttctg gatgaggtca
     1981 cgggctttgt catgctcata tccggcagca acaagggtgt cgagaagtcg tttgtctgtt
     2041 ttgttcagtt tatggcgggg tgatttgctc acatcgttct ccgttttttc atgatccaac
     2101 agttttatat ctcatcctga tttgctccgg tgttctgttt cgacgttctc aatgcctgcc
     2161 tccccatagt gtgatcctta ttgcaaaaat gaaaatccat gctcttgaca tggacaatgc
     2221 tgagtagtag gttttcactg tacataaaac cagtggttat atgtacagta tattgttttt
     2281 aatttattgt tttaattatc aaagaggaat ttctttatgg ataaagtcac tgataattct
     2341 ccagatgtgg agagcacaga atctactgag gggtcattcc caactgttgg ggttgatact
     2401 ggcgatacga ttacagcgac gcttgcaact ggaactgaaa atgttggtgg aggcggtgga
     2461 gcatttggtg gggccagtga aagttctgct gcgatacatg caaccgctaa atggtctacc
     2521 gcgcagttga aaaaacatca ggctgaacag gctgcccgtg ctgctgcggc tgaggcagca
     2581 ttggcaaaag cgaaatctca gcgtgatgcc ctgactcaac gtctcaagga tattgttaat
     2641 gacgctttac gtgctaatgc cgcccgtagt ccatcagtaa ctgaccttgc tcatgccaat
     2701 aatatggcaa tgcaggcaga ggctgagcgt ttgcgccttg cgaaggcaga gcaaaaagcc
     2761 cgtgaagaag ctgaagcagc agaaaaagcg ctccgggaag cagaacgcca acgtgatgag
     2821 attgcccgcc aacaggctga aaccgcgcat ttgttagcaa tggcggaggc agcagaggct
     2881 gagaaaaatc gacaggattc tcttgatgaa gagcatcggg ctgtggaagt ggcagagaag
     2941 aagctggctg aggctaaagc tgaactggcg aaggccgaaa gcgatgtaca gagtaagcaa
     3001 gcgattgttt ccagagttgc aggggagctt gaaaacgctc aaaaaagtgt tgatgtgaag
     3061 gttactggat ttcctggatg gcgtgatgtt cagaaaaaac tggagagaca attgcaggat
     3121 aagaagaatg aatattcgtc agtgacgaat gctcttaatt ctgcggttag cattagagat
     3181 gctaaaaaaa cagatgttca gaatgctgag ataaaattaa aagaagctaa ggatgctctt
     3241 gagaagagtc aggtaaaaga ctctgttgat actatggttg ggttttatca atatataacc
     3301 gaacaatatg gggaaaaata ttccagaata gctcaggatt tagctgaaaa ggcgaagggt
     3361 agtaaattta gtagtgttga tgaagctctt gctgcatttg aaaagtataa aaatgtactg
     3421 gataagaaaa tcagtaaggt tgatcgggat gctattttta atgcgttaga gtctgttaat
     3481 tatgatgagt tatctaagaa tttaacgaag atatcaaagt cgcttaaaat cactagtcga
     3541 gtatcttttc tatatgatgt tggttctgat tttaaaaatg caatagaaac tggtaactgg
     3601 cgacctttgt ttgtcacgct ggagaaaagt gcggtcgatg taggtgtggc aaaaattgtg
     3661 gcattaatgt ttagttttat tgttggtgtg cctcttggtt tctgggggat tgcaattgtc
     3721 acaggtattg tttcttctta cataggggat gatgagttga acaaacttaa tgagttgtta
     3781 gggatttaat ttctctttag agtgtaatat atgaaacttg gcaatcctac tgggattgcc
     3841 attaataaat aaaacaatgc agataacttg cataagtacc agggttcttt ttttaacttt
     3901 gtattttctt tgatatatct agatatggtt aatttagagt atggatatag caatacatgt
     3961 attgatgata taaatataaa taacaaagga gtgttatcat tgtcgttaaa tattaaaata
     4021 agaatccatg gtataagtga ttcaggtaaa ttatgtaggt agtattttaa atgcataagt
     4081 atttttactt ttgttggaaa gaaatatgaa aataataatt tttctaatta caatattatt
     4141 cttgtcagga tgtcaagcaa actatattcg tgatgtgcaa ggaggaaccg ttgcaccatc
     4201 ttcgtcttct gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atcttcttga
     4261 gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaaccgcc tggggaggcg
     4321 gtttagtcga aggttaagtc agttggggac tgcttaaccg ggtaactggc tttagtggag
     4381 cacagatacc aaatgctgtc cttccagtgt agccgctata aggcttcaac ttcaagagtt
     4441 ctgcgtatct aaatccacta tccagttacc aatggctgct gccagtggcg ttaagtcgtg
     4501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     4621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4681 ggtaatcggc agggtcggaa caggagagcg cacgagggag cttccggggg gaaacgcctg
     4741 gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc
     4801 tcgtcagggg ggcggagcct atggaaaaag cgcctgtgcc gcggcctttc ccggggtctt
     4861 cgtttttttg cgcacatgtt ctttcccggg ttatccccgg attctgtgga taacggggtt
     4921 accgcctttg agaaaggaga gtccgctcgc cgcagccgaa cgaccgagcg gagcgagtca
     4981 gtgagcgagg aagcggaaaa gcgcctggac gtgcattctc tccttacgca tctgtgcggc
     5041 atttcacacc cggcatggcg cacttttcat acaatccgca ctgttgccgc ataattaagc
     5101 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa
     5161 aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggcaagctg
     5221 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga
     5281 ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt
     5341 cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt ctgatgaagc
     5401 gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc catgtgtggg
     5461 gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc acgatacggg
     5521 tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat
     5581 ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg
     5641 caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag atagcccgga
     5701 agataaacag tggacagtgg tcggggcatg accgggttca tgtggtggct gcactgatgg
     5761 ccatcggacg tgaactgagt gagctgaggg atgaggtccg taaacaggga gaacgcgatg
     5821 atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga ttacctgctg
     5881 ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga agaggtgcgg
     5941 gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct gtcttttgca
     6001 gagcagaccc tgccaccggg agagcgtgaa agggtgatgg agagctttga gcgggtactg
     6061 atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaagggg
     6121 cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa acggctccag
     6181 ccctattacg accgggcgga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac
     6241 cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt caccccgaat
     6301 aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt
     6361 taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt
     6421 tttgaggtgg tgaggacaac caggagcagt atcagtatcg ccgatccgga gggcgggcgt
     6481 aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga cagacttcga
     6541 gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg agttcaggaa
     6601 gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca gagacgctat
     6661 cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc
     6721 gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc tctggaagct
     6781 ggtcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg gcgggatttt
     6841 tctggcgtca tgggggctga ttcagtggca ggaaagccgg attgcggcga acatcctggc
     6901 tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg gtgtgacgta
     6961 tcacgagggc aggaacggga aatttctggt gttgccgtcc ggggtgaagg gggaaaacaa
     7021 ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga gtatgacaga
     7081 gctggaaatg cacttactga acgccttaga gcaattgcag caggactata cgcagcggct
     7141 gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga cacgacagga
     7201 gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga gattagccgg
     7261 gcaggtggac cgcttgagtc ggttatttat cacgaacagc agataaggga tgaacaggaa
     7321 aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa
     7381 gcacgggtat ggcgtggccc gtcaatgggg atgtgacggt gagttgttcc gggaggttgc
     7441 cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt gtcgtttttg
     7501 ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc ccgcgatgtc
     7561 tgcgtatgtc atcaactgaa aagatggaaa aaaaacagtg tcatgaggtt tacgcaggca
     7621 aaaccagcgt tattcacatg gctgaatagc tatgcatacg gttaaaattt atcaggtgcg
     7681 atcgcggcag cttttcgggt ggtttgttgc catttttacc tgtcagcagc cgtgatcgcg
     7741 ctgaacgcat ttcagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctt
     7801 ccctcgtagc catcgagata aaccgcagaa atcgtctcag ccagcagcct ggattttttc
     7861 ggggtaaggt ctttgctgcg gtaatcagta cccgcaaatt gagttgtgcc ccggagtgag
     7921 tttaaaaatt cctggctgtg aaactgctga atgcgttgct gcacatcact ccgtgatttt
     7981 cctgtcgctt tatcctttgc tcccatatcc cagaactgcc ggaaaaaagg caccatcatt
     8041 cgtgttgtgt cggccttctg ggtggaaaag ttaggatcaa aaccatcgtc tgatgcataa
     8101 acgtaatgcc gggtatgttg ttccacactg ccctttggcg tgcaggctgt caggaccgtt
     8161 gccgctgtca gcagggcgat aagatacagt gtgccgccgg aacgtctcat gagtgtctcc
     8221 ttacctttaa atctttgtac tcagtgactt atattgaacc gctgactatc ttatcatcac
     8281 ttgcagccga tgctcaccgg ctgaacagtc tggcccagaa tccccgtttt ttttcctgct
     8341 ccagtgcctg cctgagctgc tctatctctt tatgtagctg ttcccgttcg gcttcctggc
     8401 gccggcgttc cgtatcctgt gcctgtttgt cctcaagcat cagtgtcagg cattgtttca
     8461 gctcattcag ctcccggaga atgcgttctg tctgctgatc atgtggattt tctgcctgtc
     8521 ccgcactgtg cctttctgct gtctcatcct gctttaattc gccgtatgcc cggatcagtt
     8581 cactggtttc aaactcccgt cgtccgtcct ggccggtacg gtaactgaca aggcctgcgc
     8641 gcatatcacg gtaaaactgt gaacgcgatc tgcctgtcat tttcagtgcg tcctggagtg
     8701 tgtgccatgc catgaagtga cagtgtccca ttggtgtctc atatcatggt ttcagtaaaa
     8761 catactgggg ccggagaacg gtaatgtttg tgctggtttt tgtggcatcg ggtgggaata
     8821 gcacgtggtg tgggtggctg tttttttgat cgttttcaca aaaacgaaaa tccacagggt
     8881 tgacagggaa aacgcagcgg cgtaatttta aactgtatat aaaaccagtg gttgtatata
     8941 cagtatgcgt cttttaacta tttgttttta atgtcaaaga ggttttctat gagtgataat
     9001 gaaggtagtg tcccgacaga aggtattgat tacggggaca caatggttgt gtggccgtca
     9061 actggacgaa ttccgggcgg tgatgtgaaa cccggtggtt catcaggtct cgctccatcc
     9121 atgcctccgg gatggggaga ttacagtcca caaggtatcg cacttgtaca aagtgttctt
     9181 tttccaggaa ttattcgccg gattatcctt gataaggaac ttgaagaggg ggactggtcg
     9241 ggatggtctg tcagtgtgca tagtccctgg ggaaacgaga aagtttctgc tgcacgaaca
     9301 gttcttgaga atggtttacg tagtggtttg ccagaaccat cacgcccggc tgctgtttct
     9361 tttgcccgtc tggagcctgc ttccggaaat gagcaaaaaa ttattcgtct tatggttaca
     9421 cagcaactgg agcaggtaac ggatattcct gtcagccagt taccagcagc gggtaataat
     9481 gtaccggtga aatatcgtct gacggacctt atgcagaacg gtacgcaata tattgctatt
     9541 atcggaggta ttccgatgac agtgccggta gttgatgccg ttccggttcc ggaccggagc
     9601 cgtccgggaa caaatattaa agatgtttac agtgcccctg tatcaccaaa tctaccggac
     9661 ctggtattaa gtgtggggca gatgaatact ccggttctgt ctaatcccga aattcaggaa
     9721 gagggcgtta ttgctgagac aggtaattat gttgaggcag gttatacgat gtccagtaat
     9781 aatcatgatg tcattgttcg ttttcctgac ggcagcggtg tttctccgtt atatatttca
     9841 actgtggaga ttcttgaccg taatggtctg agtcagcgtc aggaagccga aaataaagca
     9901 aaggatgatt tcagagtcaa gaaagaagaa gcggtagccc gggccgaagc tgaaaaagca
     9961 aaagccgagt tatttagtaa agcaggtgtg aaacagcctc ctgtatatac acaggaaatg
    10021 gtggaaaggg ctaattcagt aatgaatgag cagggaactc ttgttctgaa taacactgcc
    10081 agttctgtac aactggcgat gaccggaact ggtgtctgga cggctgctgg tgatattgca
    10141 ggcaacataa gcaagttttt cagtaatgct ctggagaaag taaccatacc cgaagtgggt
    10201 cccctgctta tgcggatttc tcttggcgct ctgtggttcc attcagaaga cgccggagca
    10261 ggaagtgata tcgtgccggg gcgagatatg gaggctatgt tctcgctgaa tgctcagatg
    10321 ttggcaggac agggcatggt cattgaacct ggtgctacga gtgttaatct gcctgttcgt
    10381 ggacaattga taaacagtaa cggacaactt gctctggatt tactgaagac agggaatgat
    10441 agtatccctg ctgcggttcc tgttcttaat gctgttcgtg atacagctac aggactggat
    10501 aaaattacgc tgccagcagt agtcggtgca ccttccagga cgattctggt aaatccggcc
    10561 ccacaacctt cggcaccaac agatacagga aatcatcaac ccgttccggt taccccagtg
    10621 catacaggta cggaggtaaa accggttgaa atgccggtaa cgacgattac tccagtctct
    10681 gatgccggtg gacttcgaga ttttatttac tggcgtcctg atgctaccgg gactggtgtt
    10741 gaagctgtct atgtgatgct taatgatcct ctggattcag ggcggttttc acgcaaacag
    10801 cttgataaaa aatataaaca tgctggtgat tttggtatta gtgatactaa aaagaatcgt
    10861 gaaactctta ctaaattcag ggatgctatt gaggaacatt tatccgataa ggatacagta
    10921 gagaaaggaa cataccgacg agaaaaagga tcaaaagttt attttaatcc taatacaaat
    10981 aatgtggtga tactaaaatc aaatggtgag ttcttatctg ggtggaaaat aaatccagat
    11041 gcggataatg ggcgaattta tttagaaaca ggtgaactat gaacaagatg gcgataatcg
    11101 atttggcgaa gtcattttta gcttcaaaaa taacagcaat ggagttttca gagcggattt
    11161 gtgttgagcg gagaaaattg tatggtgtta aggatttgtc tcaggatgta ttgaattgtg
    11221 gggaggagtt gtttatagct gctgagcgtt tcgagcctga ttctgatagg gctgattatg
    11281 aattcgatga taatggactt aggattgaag tccaatctat cctggaaaaa tttaaattat
    11341 aaatatgagt gattttaata tctggaattt aattataaat gtcagatact cactcatgac
    11401 aaggagatgt tatgaaaaaa aagatagggg tatcgattat tctcgttctt gttgttttgt
    11461 gtctgactgc ttgtcaaaat aactatgtaa gggatgttca gggaggaacg attgctccat
    11521 catcatcctc gaaactaacc ggggtagcga ttcagtgaaa aagattaaag gatcttcttg
    11581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccaac
    11641 ggtggtttgt ttgccggaac aagagctacc aactcttttt ccgaaggtaa ctggctttag
    11701 tggagcacag ataccaaatg ctgtccttcc agtgtagccg cgataaggct tcaacttcaa
    11761 gagttctgcg tatctaaatc cactatccag ttaccaatgg ctgctgccag tggcgttaag
    11821 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11881 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11941 taccaacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    12001 tatccggtaa tcggcagggt cggaacagga gagcgcacga gggagcttcc ggggggaaac
    12061 gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt cggtttttgt
    12121 gatgctcgtc aggggggcgg agcctatgga aaaagcgcct gtgccgcggc ctttcccggg
    12181 gtcttcgttt ttttgcgcac atgttctttc ccgggttatc cccggattct gtggataacg
    12241 gggttaccgc ctttgagaaa ggagagtccg ctcgccgcag ccgaacgacc gagcggagcg
    12301 agtcagtgag cgaggaagcg gaaaagcgcc tggacgtgca ttctctcctt acgcatctgt
    12361 gcggcatttc acacccggca tggcgcactt ttcatacaat ccgcactgtt gccgcataat
    12421 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    12481 gctaaaaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacaggca
    12541 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaagcg
    12601 tgcgaggcag ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc agatgtcagc
    12661 ctggtcgtcg gcgtccagtt cattcagttt ttccagcagg gtcagcgtct ggcttctgat
    12721 gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt ttctccatgt
    12781 gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga tgctcacgat
    12841 acgggtcacg gatgaggaac atgcgcggct gctggcacgt tgtgagggaa aacaacttgc
    12901 gtcatggatg cgtaaggtct gcctgggggc accgccatcg aaaacgtcag gtcttccgac
    12961 actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga atcagatagc
    13021 ccggaagata aacagtggac agtggtcggg gcatgaccgg gttcatgtgg tggctgcact
    13081 gatggccatc ggacgtgaac tgagtgagct gagggatgag gtccgtaaac agggagaacg
    13141 cgatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc
    13201 tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct cccgaagagg
    13261 tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg gttctgtctt
    13321 ttgcagagca gaccctgcca ccgggagagc gtgaaagggt gatggagagc tttgagcggg
    13381 tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca
    13441 aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc ggaaaacggc
    13501 tccagcccta ttacgaccgg gcggaccgtc cgcgaatcaa tgcctggcag acactggtga
    13561 atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca cttgtcaccc
    13621 cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg ggactggagg
    13681 ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg cttacagcgg
    13741 caggttttga ggtggtgagg acaaccagga gcagtatcag tatcgccgat ccggagggcg
    13801 ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat ggggacagac
    13861 ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatgctgaa cagcgagttc
    13921 aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa aatcagagac
    13981 gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa cagtgccgtg
    14041 aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga gctgctctgg
    14101 aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt cacgggcggg
    14161 atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccggattgc ggcgaacatc
    14221 ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac atggggtgtg
    14281 acgtatcacg agggcaggaa cgggaaattt ctggtgttgc cgtccggggt gaagggggaa
    14341 aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta agtgagtatg
    14401 acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga ctatacgcag
    14461 cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga gctgacacga
    14521 caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt gcagagatta
    14581 gccgggcagg tggaccgctt gagtcggtta tttatcacga acagcagata agggatgaac
    14641 aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa gagcaggaac
    14701 gggaagcacg ggtatggcgt ggcccgtcaa tggggatgtg acggtgagtt gttccgggag
    14761 gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct ggcgtgtcgt
    14821 ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc tcatcccgcg
    14881 atgtctgcgt atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg aggtttacgc
    14941 aggcaaaacc agcgttattc acatggctga atagctatgc atacggttaa aatttatcag
    15001 gtgcgatcgc ggcagctttt cgggtggttt gttgccattt ttacctgtca gcagccgtga
    15061 tcgcgctgaa cgcatttcag cggtgcgtac aatt
//
