LOCUS       NZ_AP027209             7136 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia coli strain JNE082640 plasmid pJNE082640-3, complete
            sequence.
ACCESSION   NZ_AP027209
VERSION     NZ_AP027209.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00566112
            Assembly: GCF_030295505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Complete Genome Sequence of Eight LEE-negative Shiga Toxin-producing
            Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome
            Patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Kubomura,A., Lee,K., Iyoda,S. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) Contact:Akiko Kubomura National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.5.0; Trycycler v. 0.5.3
            Genome Coverage                   :: 53.4x
            Sequencing Technology :: Illumina MiSeq; PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 12:16:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,993
            CDSs (total)                      :: 4,869
            Genes (coding)                    :: 4,669
            CDSs (with protein)               :: 4,669
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 100 of 200
            Pseudo Genes (incomplete)         :: 101 of 200
            Pseudo Genes (internal stop)      :: 42 of 200
            Pseudo Genes (multiple problems)  :: 39 of 200
            Pseudo Genes (short protein)      :: 3 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027209.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JNE082640"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJNE082640-3"
                     /geo_loc_name="Japan"
                     /collection_date="2008"
                     /note="National Institute of Infectious Diseases JNE082640"
     gene            join(6754..7136,1..40)
                     /locus_tag="QUE04_RS24900"
     CDS             join(6754..7136,1..40)
                     /locus_tag="QUE04_RS24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(524..757)
                     /locus_tag="QUE04_RS24905"
                     /old_locus_tag="JNE082640_p30010"
     CDS             complement(524..757)
                     /locus_tag="QUE04_RS24905"
                     /old_locus_tag="JNE082640_p30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(764..1324)
                     /locus_tag="QUE04_RS24910"
                     /old_locus_tag="JNE082640_p30020"
     CDS             complement(764..1324)
                     /locus_tag="QUE04_RS24910"
                     /old_locus_tag="JNE082640_p30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1356..1793)
                     /locus_tag="QUE04_RS24915"
                     /pseudo=""
     CDS             complement(<1356..1793)
                     /locus_tag="QUE04_RS24915"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2002..2325)
                     /locus_tag="QUE04_RS24920"
                     /old_locus_tag="JNE082640_p30030"
     CDS             complement(2002..2325)
                     /locus_tag="QUE04_RS24920"
                     /old_locus_tag="JNE082640_p30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2393..2584
                     /locus_tag="QUE04_RS24925"
                     /old_locus_tag="JNE082640_p30040"
     CDS             2393..2584
                     /locus_tag="QUE04_RS24925"
                     /old_locus_tag="JNE082640_p30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3582..3737)
                     /gene="csl"
                     /locus_tag="QUE04_RS24930"
     CDS             complement(3582..3737)
                     /gene="csl"
                     /locus_tag="QUE04_RS24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            3767..4306
                     /gene="csi"
                     /locus_tag="QUE04_RS24935"
     CDS             3767..4306
                     /gene="csi"
                     /locus_tag="QUE04_RS24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="WP_104681840.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(4334..5833)
                     /gene="csa"
                     /locus_tag="QUE04_RS24940"
                     /old_locus_tag="JNE082640_p30050"
     CDS             complement(4334..5833)
                     /gene="csa"
                     /locus_tag="QUE04_RS24940"
                     /old_locus_tag="JNE082640_p30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_024216192.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDDYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            6072..6362
                     /locus_tag="QUE04_RS24945"
                     /old_locus_tag="JNE082640_p30060"
     CDS             6072..6362
                     /locus_tag="QUE04_RS24945"
                     /old_locus_tag="JNE082640_p30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(6354..6632)
                     /locus_tag="QUE04_RS24950"
                     /old_locus_tag="JNE082640_p30070"
     CDS             complement(6354..6632)
                     /locus_tag="QUE04_RS24950"
                     /old_locus_tag="JNE082640_p30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     Typing          complement(2671..2757)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          2781..3325
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.978"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(418..2012)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2385..2587)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.39e-10"
ORIGIN
        1 ttctgcggtt tatctcgatg gatacgaggg cagacagtaa gggatttacc ataatccctt
       61 aattgtacgc accgctgaaa tgcgttcagc gcgatcacgg ctgctgacag gtaaaaatgg
      121 caacaaacca cccgaaaagc tgccgcgatc gcacctgata aattttaacc gtatgcatag
      181 ctattcagcc atgtgaataa cgctggtttt gcctgcgtaa acctcatgac actgtttttt
      241 ttccatcttt tcagttgatg acatacgcag acatcgcggg atgaggctga ggaatgagcg
      301 cgatctggca aagaggcaaa acacagcaac aaaaacgaca cgccagaatc gcgcccggat
      361 gcgtttttaa cgcgttccgg taccatctgg caacctcccg gaacaactca ccgtcacatc
      421 cccattgacg ggccacgcca tacctgtgct tcccgttcct gctcttcatg ccaggaccgc
      481 gcacgctccc gttccaggcg tgcctgcctt tcctgttcat cccttatctg ctgttcgtga
      541 taaataaccg actcaagcgg tccacctgcc cggctaatct ctgcacctgc tgactcaagt
      601 cgtcgcactg ttccctcagt tgcccgttct cctgtcgtgt cagctcgaac atatgctgca
      661 aatccgtgaa ggcgctctcc cagtctttca gccgctgcgt atagtcctgc tgcaattgct
      721 ctaaggcgtt cagtaagtgc atttccagct ctgtcatact cacttactcc ctgaccagtc
      781 ttactgcgtt cttcttctcc accgtccagt tgttttcccc cttcaccccg gacggcagca
      841 ccagaaattt cccgttcctg ccctcgtgat acgtcacacc ccatgttttt tcccggagtt
      901 tcgccagcgt ctcttcctgg tccctgatag ccaggatgtt cgccgcaatc cggctttcct
      961 gccactgaat cagcccccat gacgccagaa aaatcccgcc cgtgacgcaa agcgccgtca
     1021 gcgacgggta cagtatccgc cctttgatca gcttccagag cagctcttcc tgccgccggg
     1081 ccagttcgtt ctctgtggcg ctgaactgcg cgttcacggc actgttcagc gtctccagtt
     1141 gttctttcac cgctgctgtg tgtgcgctga tagcgtctct gattttctgc ccgtttaagt
     1201 tcagttccct gtctacagac gcttcgagct tcctgaactc gctgttcagc atgttctctg
     1261 tagagacggc acgctctttc agtttcttct cgaagtctgt ccccatttgt aaaagattgc
     1321 tcatacagcg cccctttcag cctgagatta cgcccgccct ccgggtcggc gatactgata
     1381 ctgctcctgg ttgtcctcac aacctcaaaa cctgccgctg taagcgcctc agtgacatcc
     1441 tgacgcgttt ttagcgctcc ggcatggtaa agagcctcca gtcccctcgt aatcgcttct
     1501 gcggcctcct gtttcgcttt cggcagatta ttcggggtga caagtgtccg cctgttctcc
     1561 ggtgcgttcg ggtcgtgcag cccgtaatgg tgattcacca gtgtctgcca ggcattgatt
     1621 cgcggacggt ccgcccggtc gtaatagggc tggagccgtt ttccgctcgc cagctccata
     1681 ttcgggatga caaaattcag ctcaagacgc cccttgtcct ggtgctccac ccacaggatg
     1741 ctgtactgat ttttttcaag accgggcatc agtacccgct caaagctctc catcaccctt
     1801 tcacgctctc ccggtggcag ggtctgctct gcaaaagaca gaacccccga ggtgtatttt
     1861 ttcgcaaacg gcgtggcatc gatgagttcc cgtacctctt cgggagcacc ccgcagaact
     1921 ctcacccctt cccggttacg ctcccggccc agcaggtaat caaccggacc actgccaccg
     1981 ccttttcccc tggcatgaaa cttaactatc atcccgttct ccctgtttac ggacctcatc
     2041 cctaagctca ctcagttcac gtccgatggc catcagtgca gccaccacat gaacccggtc
     2101 atgccccgac cactgtccgc tgtttatctt ccgggctatc tgattcaggt tattgccgac
     2161 cgaagcgaac tggcgcaaca gcggcggtgc cagtgtcgga agacctgacg ttttcgatgg
     2221 cggtgccccc aggcagacct tacgcatcca tgacgcaagt tgttttccct cacaacgtgc
     2281 cagcagccgc gcatgttcct catccgtgac ccgtatcgtg agcatccttt cgcgtttcac
     2341 cggtatcatt aaaaacctcc gacagactcc ccacacatgg agaaacagaa ctgtgactaa
     2401 acaggaaaaa accgccctta acatggcccg cttcatcaga agccagacgc tgaccctgct
     2461 ggaaaaactg aatgaactgg acgtcgacga ccaggctgac atctgcgaag cgcttcacga
     2521 ccacgctgac gagctttacc gcagctgcct cgcacacttc ggggatgacg gtgaaaacct
     2581 ctgacacatg cagctcccgg agacggtcac agctggcctg tgagcggatg ccgggagcag
     2641 acaagcccgt cagggcgcgt cagcgggtgt tagcgggtgt cggggcgcag ccatgaccca
     2701 gtcacgtagc gatagcggag tgtatactgg cttaatcgtg tagcatcagt gaggattgta
     2761 tgaaaagtgc accatgccgg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgctcg
     2821 tccaggcact tttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgactgcgg
     2881 cgagcggagt ctgcttgctc aaaaacggcg atacggttat ccacagaatc aggggataag
     2941 gcagaaaaga acatgtgagc aaaaaggcaa agggcaaaaa aagccgcgtc gctggcgttt
     3001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3181 gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc
     3241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3301 tatcgtctcg agtccacccc ggtaagacac gacaaagcgc cactggcagc agccattggt
     3361 aactgaaaag tggatttaga tacgcagaac tcttgaagtt gaagccttat agcggctaca
     3421 ctgaaaggac agcatttggt atctgtgctc cacttaagcc agctacccgg ttaagcagtc
     3481 cccaactgac ttaaccttcg actaaaccgc ctccccaggc ggttttttcg tttacagggc
     3541 aagagactac gacgatctgt aaaggatctc aagaagatcc tttacaggct accactacca
     3601 ccacttccca tactcactcc ggtaacagtg gaagatggtg aaacagcccc acctccagta
     3661 tctctgacat tgttgacttg gcatgctgca agcagcatta ttcctagtga aatggcacaa
     3721 ataataattt ttttcatata tacctttatt ataatcctat aggtcaatga tgattgatga
     3781 acattcaata gatatagaca atagaaaagc caataactta ttgtatttat ttatggtaat
     3841 cggagttata cctttactct gtatactagc cgtttactat acaaatccag acaatctgtt
     3901 cttgcacaca atagctacca gtactgaaaa catcccatca ataacctccg cctacaaccc
     3961 attaatgaca aaggttatgg atatttattg taaaacagcg ccttcccttg ctttgatatt
     4021 atttatcctg acatttaaaa cgagaaagct aataaaaaaa acaaaccgaa atgctgtact
     4081 tagaagttgc ttattaagtc catttgtctg tgctgttttt ttgtatttac tctgtttccg
     4141 aaaccttgag ctaacgacag caggaaggcc agccaggcta atgaccaaca atgatgcaac
     4201 actattgcta ttttatattg gcctgtactt aatcattttt ttcataagct atttcacgct
     4261 attcacacca gttaccacgt ttaaattatt aaaagaaagg cagtaaccct gccttccttt
     4321 tacccggtat ttattaatgc gcgggtttaa tgatttcttt atttagtgca tctgcaaact
     4381 tgtcatcaat taatgcacct acaactgctg cgagtagaat acctacgata ccgacagcaa
     4441 tagcaggagc cccaagagat aaggcatatg cccctaatgt ggctgaaaaa acacccaggg
     4501 ccacagcaga agcgatgcca ctgagcaccc aggactccac ctccagcatc agaggtcccc
     4561 agttcccggt ttcatagccc tcgatgcttt tctcccggac cttctcaacc ttcatcacca
     4621 catcggcaaa tttgaaagcc ttgctgatat tacccagttt atttgccata tcctgagcat
     4681 caatacttcg ccagacgttc gccagtgcat cactgtcggc cctgttaaat ttaaaccccg
     4741 gattagacaa aactttattt aatgacgcca ttgcatcatc gtagctacgg atagttttcc
     4801 cctggaaatt tttgacatca tcagcaattt ctcttgctaa cgacttataa tcatctccaa
     4861 gataatcacc gaccttatta cctatatcag agataacttc agcggcttta ttaagtacat
     4921 cactctgaat ccggtcacgg ttcatactga aagaataaaa cacatcataa gcagcttttt
     4981 tagcttgatc ctgtcgttga ggctcttcat tattaaatcc taatgcctca tatatctctt
     5041 ccggggtctt atggtcgact ttatccataa taccagcctt agctttttca ctccctacat
     5101 aaggacgaac agctgttcta gttacagata aaacattacc ataatcatca agcaccacta
     5161 aatagtggaa ctgttcaaca tcaacatcaa attttctcac attaggaggc aaacgatccc
     5221 acgcatcttc agcctgttgt tttgcttttt cctgccgctg agattcatct ttattaaaac
     5281 ctaacgcctc atatatctcc tcaggagttt tatgattgat tttttccata atacccgcct
     5341 tagctttttc actccctacg taaggacgga cacctgttcg aagaataccg atagctctac
     5401 cattatcatt aagcgttaca cgatatttaa aaccatcgat actcacatca atctctcgcg
     5461 gtaagttgtt tttatcccac ttacgagcaa cttcatcatt aggattttca gggatatagc
     5521 ccccctgaac atcactattt ccgccagccc caggaactcc atatacaggc gtttttacca
     5581 gaccatttgt gatggtaatg cgcacctgcc cattatcatc aacataaggc ggcacatccg
     5641 gattattggt tttactccat ccccaccccg tctgacttcc atgttcacga cctccattac
     5701 cagagcctga accaccaccc cagtgaaccc cactattact attattattt ccaccttgct
     5761 gattcagatt cgcccctgtt ccccccatag actcaccagc agttgggcca tatccactta
     5821 gttctttagc cataaattcc tctttgataa ttaaaacaat aaattaaaaa cagtatactg
     5881 tacatataac cactggtttt atgtacagca aaacctacta ctcaccattg tccatgtcaa
     5941 gagcatggat tttcattttt gctataagga tcacgctatg gggaggcagg cattgagaac
     6001 gtcgaaacag aacaccggag caaatcagga tgagatataa aactgttgga tcatgaaaaa
     6061 cggagaacga tgtgagcaaa tcaccccgcc ataaactgaa caaaacagac aaacgacttc
     6121 tcgacaccct tgttgctgcc ggatatgagc atgacaaagc ccgtgacctc atccagaaac
     6181 aggtttacac gctgacactg gctgatcagc gtcatgtggt cagtgaaatc agtaatggtg
     6241 tgaatcccac ccaggcttac tcggcggtat accaggcaag acgcattcgc ctcgcccgta
     6301 aatatctgaa cggtaaaaag gttatggaag aaaccgggga aaatacgccc ccatcaacgt
     6361 aaggatttct tttgccgctc cagagactcc agttttttac gcaaatcctc tcttttttgg
     6421 gcatctctgg tgccagccag ctctgctctc aactcatcga tctgaagttg tatcttcagt
     6481 ctgttactga acattttctg tctggcatta acatccgcaa caatgccgtt ttttgtcttc
     6541 tcggcctttt gttgaaaaac attgctgtcc gcatgactgg caaccgaagc agaaagaaca
     6601 ctaaaaagca ggactggcac acattttttc acgggattat tcctgactca ttgaccatca
     6661 aatcacattg ggagtaaacc gacgtatgat aagagatact cttcggagat ataactccct
     6721 gagtatcaag attaaaaacg caaggagatg tttatgagat ctgccgctac caggctgctt
     6781 ctgatacctc tgataacagc aacaatagct cttacaggat gcaccccaaa gaccagcctg
     6841 gaacgacata cccgacatta tgtttatgct tcagatgatg gatttgatcc taacttctat
     6901 acccagaaag cagacaccat acgtatgatg ctcccgttct ttcagcagtt ccgggatatg
     6961 gggatgaaag acaaagcagc cggagtatca gcagaaacgg cacagcaacg tgcaaaagaa
     7021 ttccactcag aaaaattttt tcactcactc cagagcacaa caacctttgc tggcagaaaa
     7081 tacacaaaca gcgatatgcc ttcgctgaaa aaaatgaaac taatggcaga caccat
//
