LOCUS       NZ_AP027201             6674 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain 02E060 plasmid pO26_02E060_4, complete
            sequence.
ACCESSION   NZ_AP027201
VERSION     NZ_AP027201.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562890
            Sequence Read Archive: DRR424518
            Assembly: GCF_027925845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan:Fukuoka
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 235x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925845.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:24:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,050
            CDSs (total)                      :: 5,914
            Genes (coding)                    :: 5,581
            CDSs (with protein)               :: 5,581
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 333
            CDSs (without protein)            :: 333
            Pseudo Genes (ambiguous residues) :: 0 of 333
            Pseudo Genes (frameshifted)       :: 120 of 333
            Pseudo Genes (incomplete)         :: 210 of 333
            Pseudo Genes (internal stop)      :: 78 of 333
            Pseudo Genes (multiple problems)  :: 66 of 333
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027201.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="02E060"
                     /serovar="O26:H11"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO26_02E060_4"
                     /geo_loc_name="Japan:Fukuoka"
                     /collection_date="2002"
     gene            1..2094
                     /gene="cda"
                     /locus_tag="QMG97_RS30115"
                     /old_locus_tag="F02E060_p4_01"
     CDS             1..2094
                     /gene="cda"
                     /locus_tag="QMG97_RS30115"
                     /old_locus_tag="F02E060_p4_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2091..2354
                     /locus_tag="QMG97_RS30120"
                     /old_locus_tag="F02E060_p4_02"
     CDS             2091..2354
                     /locus_tag="QMG97_RS30120"
                     /old_locus_tag="F02E060_p4_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            2447..2569
                     /locus_tag="QMG97_RS30125"
     CDS             2447..2569
                     /locus_tag="QMG97_RS30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072165042.1"
                     /translation="MILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGVAVQ"
                     /name="colicin release lysis protein"
     gene            3276..3464
                     /locus_tag="QMG97_RS30130"
                     /old_locus_tag="F02E060_p4_04"
     CDS             3276..3464
                     /locus_tag="QMG97_RS30130"
                     /old_locus_tag="F02E060_p4_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(3616..3807)
                     /locus_tag="QMG97_RS30135"
     CDS             complement(3616..3807)
                     /locus_tag="QMG97_RS30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3875..4198
                     /locus_tag="QMG97_RS30140"
                     /old_locus_tag="F02E060_p4_06"
     CDS             3875..4198
                     /locus_tag="QMG97_RS30140"
                     /old_locus_tag="F02E060_p4_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4407..>4841
                     /locus_tag="QMG97_RS30145"
                     /pseudo=""
     CDS             4407..>4841
                     /locus_tag="QMG97_RS30145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4894..5436
                     /locus_tag="QMG97_RS30150"
     CDS             4894..5436
                     /locus_tag="QMG97_RS30150"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_244575722.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAYTAAVKEQLEMLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5443..5676
                     /locus_tag="QMG97_RS30155"
     CDS             5443..5676
                     /locus_tag="QMG97_RS30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6166..6411)
                     /locus_tag="QMG97_RS30160"
                     /old_locus_tag="F02E060_p4_09"
     CDS             complement(6166..6411)
                     /locus_tag="QMG97_RS30160"
                     /old_locus_tag="F02E060_p4_09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             1..6674
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3445..3531
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          2799..2983
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          4189..5783
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgatt acgaaggtag tggtccgaca gaaggtattg attatgggca ctcgatggtc
       61 gtgtggccgt caacaggact gatttcgggt ggtgatgtga aaccaggagg ctcatcaggt
      121 atcgctccat ccatgcctcc gggatggggg gattacagcc cacagggtat cgcacttgta
      181 caaagtgttc tttttccggg aattattcgc cggattattc tggataagga acttgaagag
      241 ggagactggt cgggatggtc tgtcagtgtg catagccctt ggggaaacga gaaagtttcc
      301 gctgcacgaa cagttcttga gaatggttta cgtggtggtt tgccagaacc gtctcgcccg
      361 gctgctgttt cttttgcccg tctggagcct gcttccggaa atgagcaaaa aattattcgt
      421 cttatggtta cacagcaact ggaacaggta acggatattc cggccagcca gttaccagca
      481 gcgggtaata atgtaccggt gaaatatcgt ctgatggacc ttatgcagaa cggtacgcaa
      541 tatatggcta ttatcggagg tattccgatg acagtgccgg tagttgatgc cgttccagtt
      601 ccggaccgga gtcgtccggg aacaaatatt aaagatgttt acagtgcccc tgtatcacca
      661 aatctaccgg acctggtatt aagtgtgggt cagatgaata ctccggttct gtctaatccc
      721 gaaattcagg aagaaggagt tattgctgag acaggtaatt atgttgaggc tggttatacg
      781 atgtccagta ataatcatga tgtcattgtt cgttttcctg atggcagtgg tgtttctccg
      841 ttatatattt caactgtaga gattctggac agtaatggtc tgagtcagcg tcaggaagcc
      901 gaaaataaag caaaggatga tttcagagtc aagaaagaag aagcggtagc ccgggctgaa
      961 gctgaaaaag caaaagccga gttatttagt aaagcaggtg tgaaccagcc tcctgtatat
     1021 acacaggaaa tgatggaaag ggctaattca gtaatgaatg agcagggagc tcttgttctg
     1081 aataacactg ccagttctgt acaactggcg atgaccggaa ctggtgtctg gactgctgct
     1141 ggtgatattg caggcaacat aagcaagttt ttcagtaatg ctctggagaa agtaaccata
     1201 cccgaagtga gtcccctgct tatgcggatt tctcttggtg ctctgtggtt tcattcagaa
     1261 gaagctggag caggaagtga tatcgtgccg gggcgaaatc tggaggcgat gttttcgctg
     1321 agtgctcaga tgttggctgg acagggcgtg gtcattgaac ctggtgcgac gagtgtaaat
     1381 ctgcctgttc gtggacaatt gataaacagt aacgggcaat tagctctgga tttactgaaa
     1441 acagggaatg aaagtatccc agctgcggtt cctgttctta atgctgtccg tgatacagca
     1501 acaggactgg ataaaatcac gttaccagca gtagtaggcg caccttcccg gacgattctg
     1561 gttaatccgg taccacaacc ttcggtgcca acagatacag gtaatcatca acccgttccg
     1621 gttacaccag tgcatacagg aacggaagta aaaccggtcg aaatgccagt aacgacgatt
     1681 actcctgttt ctgatgttgg tggactacga gattttattt actggcgtcc tgatgctgct
     1741 gggactggtg ttgaagctgt ttatgtgatg cttaatgatc ctctggattc agggcgattt
     1801 tcgcgtaaac aacttgataa aaaatataaa catgctggtg attttggtat tagtgataca
     1861 aaaaagaatc gtgaaactct tactaaattc agggatgcta ttgaggagca tttatcggat
     1921 aaggatacag tagagaaagg aacataccga agagaaaaag gttcaaaagt ttattttaat
     1981 cctaatacga tgaatgtggt tataattaag tcaaatggtg agttcttatc tgggtggaaa
     2041 ataaatccag atgcggataa tggtcgaatt tatttagaga caggtgaact atgaacaaga
     2101 tggcaatgat cgatttggcg aaattatttt tagcttcgaa aattacagca attgagtttt
     2161 cagagcgaat ttgtgttgaa cggagaagat tgtatggtgt taaggatttg tctccgaata
     2221 tattaaattg tggggaagag ttgtttatgg ctgctgagcg atttgagcct gatgcagata
     2281 gggctgatta tgaaattgat gataatggac ttaaggttga ggtccgatct atcttggaaa
     2341 aatttaaatt ataatcatga gtgattttaa tatctggcat ttaagtataa atgtcagata
     2401 ttcactcatg acaaggagat gtcatgaaaa aaagataggg ggatctatga ttctcgttct
     2461 tgctgttttg tgtctgactg cttgccaagc taactatgta agggatgttc agggtggaac
     2521 aattgctcca tcatcatctt cgaaactaat cggggtggcg gttcagtgaa aaatattaaa
     2581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccc
     2641 gccttgtggc gggtccttcc gtccgatcag aagtcttcga cctcttctaa tcggcggtgg
     2701 taactggctt ggaggagcac agtcaccaaa actgtctttt cagtttagcc ttaactggtt
     2761 cataacttca agagcaactc ctctaaatca gttaccaatg gctgctgcca gtggcgttaa
     2821 gtcgtgtctt ttccgggttg gccccaagag aacagttacc ggagaaggtg cagcagtcgg
     2881 gctgaacggg gggttcctgc atacagccca gcccggagcg aactgcctaa gtcgccccga
     2941 gataccagaa gacgtgagct atgagaaagc gccacgtgtc ccgcaaggga gaaaggcgga
     3001 aaaggtatcc ggtaatcgat atggcgggaa cgtgggggcg caggagggag cgattcaccg
     3061 gaaacggtga tgatctttaa gccacgtcgg gtttcgccac cgctggcgga tttcatggtt
     3121 gagcctcacg gctcccacag atgcaccgaa aaagtgtctg ttttgtgaag tccgtcaggg
     3181 gggcggagcc tatggaaaaa gcgcctccgg cgcggccttt ttcggggttt tcgtttcttg
     3241 ctcacatgtt ctttccggcg ttatcccctg attctgtgga taacactatc accgcttttg
     3301 agtgggcaga taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg
     3361 aagcggaaaa gcgcctggac gagcattttc tccttacgca tctgtgcggc atttcacacc
     3421 cggcatggtg cacttttcat accatcctca ctgatgccac atgattaagc cagtatacac
     3481 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga
     3541 cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggcaagctg tgaccgtctc
     3601 cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg
     3661 gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt cgtcggcgtc
     3721 cagttcattc agtttttcca gcagggtcag cgtctggctt ctgatgaagc gggccatgtt
     3781 aagggcggtt ttttcctgtt tagtcacagt tctgtttctc catgtgtggg gagtctgtcg
     3841 gaggttttta atgataccgg tgaaacgcga aaggatgctc acgatacggg tcacggatga
     3901 ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa
     3961 ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg caccgccgct
     4021 gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag atagcccgga agataaacag
     4081 tggacagtgg tcggggcatg accgggttca tgtggtggct gcactgatgg ccatcggacg
     4141 tgaactgagt gagctgaggg atgaggtccg taaacaggga gaacgggatg atagttaagt
     4201 ttcatgccag gggaaaaggc ggtggcagtg gtccggttga ttacctgctg ggccgggagc
     4261 gtaaccggga aggggcgaga gttctgcggg gtgctcccga agaggtgcgg gaactcatcg
     4321 atgcgacgcc gtttgcgaaa aaatacacct cgggggttct gtcttttgca gagcagaccc
     4381 tgccaccggg agagcgtgaa agggtgatgg agagctttga gcgggtactg atgcccggtc
     4441 ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaagggg cgtcttgagc
     4501 tgaattttgt catcccgaat atggagctgg cgagcggaaa acggctccag ccctattacg
     4561 accgggcgga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac cattacgggc
     4621 tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt caccccgaat aatctgccga
     4681 aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt taccatgccg
     4741 gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt tttgaggttg
     4801 tgaggacaac caggagcagt atcagtatcg ccgacccgga gggtgggcgt aatctcaggc
     4861 tgaaaggggc gctgtatgag caatctttta caaatgggga cagacttcga gaagaaactg
     4921 aaagagcgtg ccgtctctac agagaacatg ctgaacagcg agttcaggaa gctcgaagcg
     4981 tctgtagaca gggaactgaa cttaaacggg cagaaaatca gagacgctat cagcgcatac
     5041 acagcagcgg tgaaagaaca actggagatg ctgaacagtg ccgtgaacgc gcagttcagc
     5101 gccacagaga acgaacttgc ccggcggcag gaagagctgc tctggaagct ggtcaaaggg
     5161 cggatactgt acccgtcgct gacggcgctt tgcgtcacgg tcgggatttt tctggcgtca
     5221 tgggggctga ttcagtggca ggaaagccgt attgcggcga acatcctggc tatcagggac
     5281 caggaagaga cgctggcgaa actccgggaa aaaacatggg gtgtgacgta tcacgagggc
     5341 aggaacggga aatttctggt gctgccgtcc ggggtgaagg gggaaaacaa ctggacggtg
     5401 gagaagaaga acgcagtaag actggtcagg gagtaagtga gtatgacaga gctggaaatg
     5461 cacttactga acgccttaga gcaattgcag caggactata cgcagcggct gaaagactgg
     5521 gagagcgcct tcacggattt gcagcatatg ttcgagctga cacgacagga gaacgggcaa
     5581 ctgagggaac agtgcgacga cttgagtcag caggtgcaga gattagccgg gcaggtggac
     5641 cgcttgagtc ggttatttat cacgaacagc agataaggga tgaacaggaa aggcaggcac
     5701 gcctggaacg ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa gcacaggtat
     5761 ggcgtggccc gtcaatgggg atgtgacggt gagttgttcc gggaggttgc cagatggtac
     5821 cggaacgcgt taaaaacgca tccgggcgcg attctggcgt gtcgtttttg ttgctgtgtt
     5881 ttgcctcttt gccagatcgc gctcattcct cagcctcatc ccgcgatgtc tgcgtatgtc
     5941 atcaactgaa aagatggaaa aaaaacagtg tcatgaggtt tacgcaggca aaaccagcgt
     6001 tattcacatg gctgaatagc tatgcatacg gttaaaattt atcaggtgcg atcgcggcag
     6061 cttttcgggt ggtttgttgc catttttacc tgtcagcagc cgtgatcgcg ctgaacgcat
     6121 ttcagcggtg cgtacaataa ggggttatgt taaataaccc ttagcttagc ggcggcgcag
     6181 tggttgccag gtgaaggtgt catccgggtc ctgtccggca gtcagtgtgg cttctcgatt
     6241 tttgctggcc gttcgttcat caagaatggc tttaagtatc cgtgcccgga ggcgtcgctt
     6301 tgtttcctgc ggcattttgt cctttttcgt cagccccatg gcacgagctt cagcggcaat
     6361 ggcttctgcc agtcggcgct cttcggcttc ggtcagtcta tggcgtttca tggtggtctc
     6421 cgggtgtttc tctggtgtca taacagttta actgttggct ctctccttat gctaatcagc
     6481 cttctttcat gcctgttgcc attgagttta agaatgattg gttgtttttc ttgagcgttt
     6541 tcacaaaaac gtaaatccag tgggttgaca ggatttacga ggtgacgtaa ctttttactg
     6601 tacataaaac cagtggtttt atgtacagtt tgttgttttt aacgcattgt tttaaaagtc
     6661 aaagaggtgt tttt
//
