LOCUS       NZ_AP027196             6675 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain 10153 plasmid pO26_10153_5, complete
            sequence.
ACCESSION   NZ_AP027196
VERSION     NZ_AP027196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562889
            Sequence Read Archive: DRR424517
            Assembly: GCF_027925825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan:Miyazaki
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 58x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925825.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,352
            CDSs (total)                      :: 6,209
            Genes (coding)                    :: 5,858
            CDSs (with protein)               :: 5,858
            Genes (RNA)                       :: 143
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 110
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 351
            CDSs (without protein)            :: 351
            Pseudo Genes (ambiguous residues) :: 0 of 351
            Pseudo Genes (frameshifted)       :: 134 of 351
            Pseudo Genes (incomplete)         :: 217 of 351
            Pseudo Genes (internal stop)      :: 79 of 351
            Pseudo Genes (multiple problems)  :: 69 of 351
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027196.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10153"
                     /serovar="O26:H11"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO26_10153_5"
                     /geo_loc_name="Japan:Miyazaki"
                     /collection_date="1999"
     gene            1..2094
                     /gene="cda"
                     /locus_tag="QMG89_RS31595"
                     /old_locus_tag="M10153_p5_01"
     CDS             1..2094
                     /gene="cda"
                     /locus_tag="QMG89_RS31595"
                     /old_locus_tag="M10153_p5_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            2091..2354
                     /locus_tag="QMG89_RS31600"
                     /old_locus_tag="M10153_p5_02"
     CDS             2091..2354
                     /locus_tag="QMG89_RS31600"
                     /old_locus_tag="M10153_p5_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            2424..2570
                     /locus_tag="QMG89_RS31605"
                     /old_locus_tag="M10153_p5_03"
     CDS             2424..2570
                     /locus_tag="QMG89_RS31605"
                     /old_locus_tag="M10153_p5_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            3277..3465
                     /locus_tag="QMG89_RS31610"
                     /old_locus_tag="M10153_p5_05"
     CDS             3277..3465
                     /locus_tag="QMG89_RS31610"
                     /old_locus_tag="M10153_p5_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(3617..3808)
                     /locus_tag="QMG89_RS31615"
     CDS             complement(3617..3808)
                     /locus_tag="QMG89_RS31615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3876..4199
                     /locus_tag="QMG89_RS31620"
                     /old_locus_tag="M10153_p5_07"
     CDS             3876..4199
                     /locus_tag="QMG89_RS31620"
                     /old_locus_tag="M10153_p5_07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4408..>4842
                     /locus_tag="QMG89_RS31625"
                     /pseudo=""
     CDS             4408..>4842
                     /locus_tag="QMG89_RS31625"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4895..5437
                     /locus_tag="QMG89_RS31630"
     CDS             4895..5437
                     /locus_tag="QMG89_RS31630"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_235777954.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLEMLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5444..5677
                     /locus_tag="QMG89_RS31635"
     CDS             5444..5677
                     /locus_tag="QMG89_RS31635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6167..6412)
                     /locus_tag="QMG89_RS31640"
                     /old_locus_tag="M10153_p5_10"
     CDS             complement(6167..6412)
                     /locus_tag="QMG89_RS31640"
                     /old_locus_tag="M10153_p5_10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3446..3532
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          2800..2984
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          4190..5784
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgatt acgaaggtag tggtccgaca gaaggtattg attatgggca ctcgatggtc
       61 gtgtggccgt caacaggact gatttcgggt ggtgatgtga aaccaggagg ctcatcaggt
      121 atcgctccat ccatgcctcc gggatggggg gattacagcc cacagggtat cgcacttgta
      181 caaagtgttc tttttccggg aattattcgc cggattattc tggataagga acttgaagag
      241 ggagactggt cgggatggtc tgtcagtgtg catagccctt ggggaaacga gaaagtttcc
      301 gctgcacgaa cagttcttga gaatggttta cgtggtggtt tgccagaacc gtctcgcccg
      361 gctgctgttt cttttgcccg tctggagcct gcttccggaa atgagcaaaa aattattcgt
      421 cttatggtta cacagcaact ggaacaggta acggatattc cggccagcca gttaccagca
      481 gcgggtaata atgtaccggt gaaatatcgt ctgatggacc ttatgcagaa cggtacgcaa
      541 tatatggcta ttatcggagg tattccgatg acagtgccgg tagttgatgc cgttccagtt
      601 ccggaccgga gtcgtccggg aacaaatatt aaagatgttt acagtgcccc tgtatcacca
      661 aatctaccgg acctggtatt aagtgtgggt cagatgaata ctccggttct gtctaatccc
      721 gaaattcagg aagaaggagt tattgctgag acaggtaatt atgttgaggc tggttatacg
      781 atgtccagta ataatcatga tgtcattgtt cgttttcctg atggcagtgg tgtttctccg
      841 ttatatattt caactgtaga gattctggac agtaatggtc tgagtcagcg tcaggaagcc
      901 gaaaataaag caaaggatga tttcagagtc aagaaagaag aagcggtagc ccgggctgaa
      961 gctgaaaaag caaaagccga gttatttagt aaagcaggtg tgaaccagcc tcctgtatat
     1021 acacaggaaa tgatggaaag ggctaattca gtaatgaatg agcagggagc tcttgttctg
     1081 aataacactg ccagttctgt acaactggcg atgaccggaa ctggtgtctg gactgctgct
     1141 ggtgatattg caggcaacat aagcaagttt ttcagtaatg ctctggagaa agtaaccata
     1201 cccgaagtga gtcccctgct tatgcggatt tctcttggtg ctctgtggtt tcattcagaa
     1261 gaagctggag caggaagtga tatcgtgccg gggcgaaatc tggaggcgat gttttcgctg
     1321 agtgctcaga tgttggctgg acagggcgtg gtcattgaac ctggtgcgac gagtgtaaat
     1381 ctgcctgttc gtggacaatt gataaacagt aacgggcaat tagctctgga tttactgaaa
     1441 acagggaatg aaagtatccc agctgcggtt cctgttctta atgctgtccg tgatacagca
     1501 acaggactgg ataaaatcac gttaccagca gtagtaggcg caccttcccg gacgattctg
     1561 gttaatccgg taccacaacc ttcggtgcca acagatacag gtaatcatca acccgttccg
     1621 gttacaccag tgcatacagg aacggaagta aaaccggtcg aaatgccagt aacgacgatt
     1681 actcctgttt ctgatgttgg tggactacga gattttattt actggcgtcc tgatgctgct
     1741 gggactggtg ttgaagctgt ttatgtgatg cttaatgatc ctctggattc agggcgattt
     1801 tcgcgtaaac aacttgataa aaaatataaa catgctggtg attttggtat tagtgataca
     1861 aaaaagaatc gtgaaactct tactaaattc agggatgcta ttgaggagca tttatcggat
     1921 aaggatacag tagagaaagg aacataccga agagaaaaag gttcaaaagt ttattttaat
     1981 cctaatacga tgaatgtggt tataattaag tcaaatggtg agttcttatc tgggtggaaa
     2041 ataaatccag atgcggataa tggtcgaatt tatttagaga caggtgaact atgaacaaga
     2101 tggcaatgat cgatttggcg aaattatttt tagcttcgaa aattacagca attgagtttt
     2161 cagagcgaat ttgtgttgaa cggagaagat tgtatggtgt taaggatttg tctccgaata
     2221 tattaaattg tggggaagag ttgtttatgg ctgctgagcg atttgagcct gatgcagata
     2281 gggctgatta tgaaattgat gataatggac ttaaggttga ggtccgatct atcttggaaa
     2341 aatttaaatt ataatcatga gtgattttaa tatctggcat ttaagtataa atgtcagata
     2401 ttcactcatg acaaggagat gtcatgaaaa aaaagatagg gggatctatg attctcgttc
     2461 ttgctgtttt gtgtctgact gcttgccaag ctaactatgt aagggatgtt cagggtggaa
     2521 caattgctcc atcatcatct tcgaaactaa tcggggtggc ggttcagtga aaaatattaa
     2581 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc
     2641 cgccttgtgg cgggtccttc cgtccgatca gaagtcttcg acctcttcta atcggcggtg
     2701 gtaactggct tggaggagca cagtcaccaa aactgtcttt tcagtttagc cttaactggt
     2761 tcataacttc aagagcaact cctctaaatc agttaccaat ggctgctgcc agtggcgtta
     2821 agtcgtgtct tttccgggtt ggccccaaga gaacagttac cggagaaggt gcagcagtcg
     2881 ggctgaacgg ggggttcctg catacagccc agcccggagc gaactgccta agtcgccccg
     2941 agataccaga agacgtgagc tatgagaaag cgccacgtgt cccgcaaggg agaaaggcgg
     3001 aaaaggtatc cggtaatcga tatggcggga acgtgggggc gcaggaggga gcgattcacc
     3061 ggaaacggtg atgatcttta agccacgtcg ggtttcgcca ccgctggcgg atttcatggt
     3121 tgagcctcac ggctcccaca gatgcaccga aaaagtgtct gttttgtgaa gtccgtcagg
     3181 ggggcggagc ctatggaaaa agcgcctccg gcgcggcctt tttcggggtt ttcgtttctt
     3241 gctcacatgt tctttccggc gttatcccct gattctgtgg ataacactat caccgctttt
     3301 gagtgggcag ataccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     3361 gaagcggaaa agcgcctgga cgagcatttt ctccttacgc atctgtgcgg catttcacac
     3421 ccggcatggt gcacttttca taccatcctc actgatgcca catgattaag ccagtataca
     3481 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcta aaacccgctg
     3541 acgcgccctg acgggcttgt ctgctcccgg catccgctca caggcaagct gtgaccgtct
     3601 ccgggagctg catgtgtcag aggttttcac cgtcatcccc gaagcgtgcg aggcagctgc
     3661 ggtaaagctc gtcagcgtgg tcgtgaagcg cttcgcagat gtcagcctgg tcgtcggcgt
     3721 ccagttcatt cagtttttcc agcagggtca gcgtctggct tctgatgaag cgggccatgt
     3781 taagggcggt tttttcctgt ttagtcacag ttctgtttct ccatgtgtgg ggagtctgtc
     3841 ggaggttttt aatgataccg gtgaaacgcg aaaggatgct cacgatacgg gtcacggatg
     3901 aggaacatgc gcggctgctg gcacgttgtg agggaaaaca acttgcgtca tggatgcgta
     3961 aggtctgcct gggggcaccg ccatcgaaaa cgtcaggtct tccgacactg gcaccgccgc
     4021 tgttgcgcca gttcgcttcg gtcggcaata acctgaatca gatagcccgg aagataaaca
     4081 gtggacagtg gtcggggcat gaccgggttc atgtggtggc tgcactgatg gccatcggac
     4141 gtgaactgag tgagctgagg gatgaggtcc gtaaacaggg agaacgggat gatagttaag
     4201 tttcatgcca ggggaaaagg cggtggcagt ggtccggttg attacctgct gggccgggag
     4261 cgtaaccggg aaggggcgag agttctgcgg ggtgctcccg aagaggtgcg ggaactcatc
     4321 gatgcgacgc cgtttgcgaa aaaatacacc tcgggggttc tgtcttttgc agagcagacc
     4381 ctgccaccgg gagagcgtga aagggtgatg gagagctttg agcgggtact gatgcccggt
     4441 cttgaaaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg gcgtcttgag
     4501 ctgaattttg tcatcccgaa tatggagctg gcgagcggaa aacggctcca gccctattac
     4561 gaccgggcgg accgtccgcg aatcaatgcc tggcagacac tggtgaatca ccattacggg
     4621 ctgcacgacc cgaacgcacc ggagaacagg cggacacttg tcaccccgaa taatctgccg
     4681 aaagcgaaac aggaggccgc agaagcgatt acgaggggac tggaggctct ttaccatgcc
     4741 ggagcgctaa aaacgcgtca ggatgtcact gaggcgctta cagcggcagg ttttgaggtt
     4801 gtgaggacaa ccaggagcag tatcagtatc gccgacccgg agggtgggcg taatctcagg
     4861 ctgaaagggg cgctgtatga gcaatctttt acaaatgggg acagacttcg agaagaaact
     4921 gaaagagcgt gccgtctcta cagagaacat gctgaacagc gagttcagga agctcgaagc
     4981 gtctgtagac agggaactga acttaaacgg gcagaaaatc agagacgcta tcagcgcaca
     5041 cacagcagcg gtgaaagaac aactggagat gctgaacagt gccgtgaacg cgcagttcag
     5101 cgccacagag aacgaacttg cccggcggca ggaagagctg ctctggaagc tggtcaaagg
     5161 gcggatactg tacccgtcgc tgacggcgct ttgcgtcacg gtcgggattt ttctggcgtc
     5221 atgggggctg attcagtggc aggaaagccg tattgcggcg aacatcctgg ctatcaggga
     5281 ccaggaagag acgctggcga aactccggga aaaaacatgg ggtgtgacgt atcacgaggg
     5341 caggaacggg aaatttctgg tgctgccgtc cggggtgaag ggggaaaaca actggacggt
     5401 ggagaagaag aacgcagtaa gactggtcag ggagtaagtg agtatgacag agctggaaat
     5461 gcacttactg aacgccttag agcaattgca gcaggactat acgcagcggc tgaaagactg
     5521 ggagagcgcc ttcacggatt tgcagcatat gttcgagctg acacgacagg agaacgggca
     5581 actgagggaa cagtgcgacg acttgagtca gcaggtgcag agattagccg ggcaggtgga
     5641 ccgcttgagt cggttattta tcacgaacag cagataaggg atgaacagga aaggcaggca
     5701 cgcctggaac gggagcgtgc gcggtcctgg catgaagagc aggaacggga agcacaggta
     5761 tggcgtggcc cgtcaatggg gatgtgacgg tgagttgttc cgggaggttg ccagatggta
     5821 ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg tgtcgttttt gttgctgtgt
     5881 tttgcctctt tgccagatcg cgctcattcc tcagcctcat cccgcgatgt ctgcgtatgt
     5941 catcaactga aaagatggaa aaaaaacagt gtcatgaggt ttacgcaggc aaaaccagcg
     6001 ttattcacat ggctgaatag ctatgcatac ggttaaaatt tatcaggtgc gatcgcggca
     6061 gcttttcggg tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc gctgaacgca
     6121 tttcagcggt gcgtacaata aggggttatg ttaaataacc cttagcttag cggcggcgca
     6181 gtggttgcca ggtgaaggtg tcatccgggt cctgtccggc agtcagtgtg gcttctcgat
     6241 ttttgctggc cgttcgttca tcaagaatgg ctttaagtat ccgtgcccgg aggcgtcgct
     6301 ttgtttcctg cggcattttg tcctttttcg tcagccccat ggcacgagct tcagcggcaa
     6361 tggcttctgc cagtcggcgc tcttcggctt cggtcagtct atggcgtttc atggtggtct
     6421 ccgggtgttt ctctggtgtc ataacagttt aactgttggc tctctcctta tgctaatcag
     6481 ccttctttca tgcctgttgc cattgagttt aagaatgatt ggttgttttt cttgagcgtt
     6541 ttcacaaaaa cgtaaatcca gtgggttgac aggatttacg aggtgacgta actttttact
     6601 gtacataaaa ccagtggttt tatgtacagt ttgttgtttt taacgcattg ttttaaaagt
     6661 caaagaggtg ttttt
//
