LOCUS       NZ_AP027195            10915 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain 10153 plasmid pO26_10153_4, complete
            sequence.
ACCESSION   NZ_AP027195
VERSION     NZ_AP027195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562889
            Sequence Read Archive: DRR424517
            Assembly: GCF_027925825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan:Miyazaki
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 58x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925825.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,352
            CDSs (total)                      :: 6,209
            Genes (coding)                    :: 5,858
            CDSs (with protein)               :: 5,858
            Genes (RNA)                       :: 143
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 110
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 351
            CDSs (without protein)            :: 351
            Pseudo Genes (ambiguous residues) :: 0 of 351
            Pseudo Genes (frameshifted)       :: 134 of 351
            Pseudo Genes (incomplete)         :: 217 of 351
            Pseudo Genes (internal stop)      :: 79 of 351
            Pseudo Genes (multiple problems)  :: 69 of 351
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027195.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10153"
                     /serovar="O26:H11"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO26_10153_4"
                     /geo_loc_name="Japan:Miyazaki"
                     /collection_date="1999"
     gene            1..894
                     /locus_tag="QMG89_RS31540"
                     /old_locus_tag="M10153_p4_01"
     CDS             1..894
                     /locus_tag="QMG89_RS31540"
                     /old_locus_tag="M10153_p4_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277131.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(1110..1385)
                     /locus_tag="QMG89_RS31545"
                     /old_locus_tag="M10153_p4_02"
     CDS             complement(1110..1385)
                     /locus_tag="QMG89_RS31545"
                     /old_locus_tag="M10153_p4_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            1564..3783
                     /gene="mobQ"
                     /locus_tag="QMG89_RS31550"
                     /old_locus_tag="M10153_p4_03"
     CDS             1564..3783
                     /gene="mobQ"
                     /locus_tag="QMG89_RS31550"
                     /old_locus_tag="M10153_p4_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_047090469.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAQRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGVCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4016..>5566
                     /gene="col"
                     /locus_tag="QMG89_RS31555"
                     /old_locus_tag="M10153_p4_04"
                     /pseudo=""
     CDS             4016..>5566
                     /gene="col"
                     /locus_tag="QMG89_RS31555"
                     /old_locus_tag="M10153_p4_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /name="col"
     gene            5576..5719
                     /locus_tag="QMG89_RS31560"
     CDS             5576..5719
                     /locus_tag="QMG89_RS31560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            6143..>6349
                     /locus_tag="QMG89_RS31565"
                     /pseudo=""
     CDS             6143..>6349
                     /locus_tag="QMG89_RS31565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /name="plasmid replication protein"
     gene            complement(<6346..6582)
                     /gene="mobC"
                     /locus_tag="QMG89_RS31570"
                     /pseudo=""
     CDS             complement(<6346..6582)
                     /gene="mobC"
                     /locus_tag="QMG89_RS31570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /name="mobC"
     gene            6761..7940
                     /gene="mobQ"
                     /locus_tag="QMG89_RS31575"
                     /pseudo=""
     CDS             6761..7940
                     /gene="mobQ"
                     /locus_tag="QMG89_RS31575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     gene            8173..9918
                     /gene="col"
                     /locus_tag="QMG89_RS31580"
                     /old_locus_tag="M10153_p4_08"
     CDS             8173..9918
                     /gene="col"
                     /locus_tag="QMG89_RS31580"
                     /old_locus_tag="M10153_p4_08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_012537772.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNSNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQ
                     FKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHID
                     IHRGK"
                     /name="col"
     gene            9921..10181
                     /locus_tag="QMG89_RS31585"
                     /old_locus_tag="M10153_p4_09"
     CDS             9921..10181
                     /locus_tag="QMG89_RS31585"
                     /old_locus_tag="M10153_p4_09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            10243..10386
                     /locus_tag="QMG89_RS31590"
                     /old_locus_tag="M10153_p4_10"
     CDS             10243..10386
                     /locus_tag="QMG89_RS31590"
                     /old_locus_tag="M10153_p4_10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..10915
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1390..1563)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1564..1922)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6587..6760)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.286"
                     /coverage="98.0"
                     /evalue="2.6299999999999998e-81"
     Typing          6761..7119
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="100.0"
                     /evalue="3.97e-172"
     Typing          2..894
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.387"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1565..3780
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.729"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6675..7474
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.127"
                     /coverage="99.0"
                     /evalue="7.45e-169"
ORIGIN
        1 atgagcgccg tacttcaacg cttcagggaa aaattaccgc acaaaccgta ctgtacgaac
       61 gatttcgcgt acggcgttcg cattctgccg aaaaacattg ccattcttgc ccgtttcatc
      121 cagcagaacc agccacatgc actgtactgg cttccctttg acgtggaccg gatgggggca
      181 tcaatcgact ggagcgaccg gaattgtccg gccccgaaca tcaccgtaaa aaatccccgt
      241 aacgggcacg cgcatctgct ctacgcgctc gcccttcctg tgagaactgc gccggatgca
      301 tcggcttcgg cgctcagata cgctgccgct attgagcgtg cgttgtgtga aaaactgggc
      361 gcggatgtga attacagcgg cctgatctgc aaaaatccgt gccaccctga atggcaggaa
      421 gtggaatggc gcgaggaacc ctacactctc gacgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gccgtagcgt cgataaaaat tacgggctgg ggcgaaacta ccatctgttc
      541 gaaaaggtcc gtaaatgggc ctacagagcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tctccggctg aatgtcgggc tattggcaag agcattgcga aatatacaca caggaaattc
      721 tcaccagagg gattttccgc tgtacaggcc gctcgaggtc gcaagggcgg aactaaatct
      781 aagcgcgcag cagttcctac atcagcacgt tcgctgaagc cgtgggaggc attagggatc
      841 agtcgagcga cgtactaccg aaaattaaaa tgtgatccag atctcgcaaa atgagaccag
      901 ataagcctta tcagataaca gcgccctttt ggcgtctttt tgagcagtga tctttcttgc
      961 gattttctcc gttctgcccc gtaatggcgt ggctttccgc atgattgagg tggtagcgct
     1021 cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg aggaagcgca aaggcgtccg
     1081 gtggtgcatg ggcacttacg cgccggggct tagtggttct gcggtttcgc cggtggtctg
     1141 ggtagcatct ccagctcgtt aatcagcggt tgtagtcggt tcacatccgc ctgtcttgtg
     1201 acttcctttc gcagaaactg gagcaggaac gcacgcagtt gcgcttcttc cggcctccgt
     1261 accctcgcca gcatggcagc ccccacaatg actttttgcg ccgtgtccag gctccggctc
     1321 ttcgccttca ggcgctgtaa tctggcctca gcttcggcaa tcttctgttc gagtgttctg
     1381 ctcatttcgt gactccgtgc gcggtgaaaa atcgcatttt agcgcgtcac tggtagttgc
     1441 aaaactgaac tggcataatg cacggcacat cacgaagtgc gcacttatac aatctccact
     1501 tcgtttcgat tgtgtgcggt ctgcgacgct aaaagaaaac ggcaaaaagg cattacggca
     1561 gaaatggcga tttatcatct cagcatgaaa atcatttcgc gaaaaaacgg ctacagtgct
     1621 gttgcttctg ctgcctaccg ttccggctct gtcatacccg atgaccgtac cggattaatc
     1681 cacgattaca cccgtaaacg cggcgttgat gatgcggtca ttctcacccc tgcgaatgca
     1741 ccgtcctggt gtggtgaccg ttccgttctc tggaatgcgg tcgagaaagc cgaacagcga
     1801 cggaactccc agctggcaag ggagattgaa ctcgccattc cccgtgagat ttcccgcgag
     1861 gccgcacggg agaccgttct cgcttttgtc cgggaaaact ttgtcagtcg gggcatgatt
     1921 gccgatgtgg cgttccatca catggaccgg accaatcccc atgcgcacat catgctgacc
     1981 acgagagctg tcggggaaac gggattcgca ggaaaggtca gggactggaa cgaccgggca
     2041 ctcgccgaga cgtggcgcgc atcatgggct gaccatgcga acagagcgct tgcgaacgcc
     2101 ggctaccagg aagagataga ccatcgttca tacgagcgtc agggactgga gaaagcgccg
     2161 ggccttcacc tcggaaaggc tgcctgtgcg atggaaaaac ggggaatgga aacagaacgc
     2221 ggtgagcaga accgtctgat taacagcctt aacctggaaa tacaggtttc ccgcacgcag
     2281 cttgctctca ggacggttca ggaagcgcag cgtaagcggg aactcagcga tgctgcacgt
     2341 cgtgcagcgg aagcccttaa cctgaccatt cccgctgcga atgcctcagc ggataccctg
     2401 cgggaattca ttgccacgct gccgcaggaa tgcgggaacg cgtgggagat gaccccggag
     2461 ttcctggcga tgagcgggaa ggtgaacgac atcgaacgtg aggggaatgc gctgctgaaa
     2521 gagcaggcca ttctcgaaaa ggagatgacc ggactcaaaa aagcacgccc tgtcgcgtcc
     2581 cttctgtcag agattcccct gatgacatgg gctgaaccgg aataccgcaa aagacaactc
     2641 cgtttctgga aactcgggaa acagattgaa tctcttcgcc gcacctacag ggccgtgaaa
     2701 gaacgggaca ttcccgcccg tcgtcaggcc tttgaaacgc agtggaatac gtggattgcg
     2761 ccgggaatgg cagagctgaa agaaaaactg tcagcacggg aagcggagcg gcgcagggag
     2821 gaagccgaag cggaagcgcg ccggaaggaa caggagcatg aggcgcggct gaaacgtcat
     2881 gataaccacc gtctgagccg tgaaacggca ttagtcgggg ttattacgga gctggggcgt
     2941 gccagagagc cgggaacggg caggataacc cgctacatga tgctgagtaa cagagccgga
     3001 gaattcacgg tatggggtga tgagctggcg cattaccccc agagtgttca tgacccggtg
     3061 aatgtttacc tgtcgccagg cggggctgtg atggtctcgg atatacgtga gggaatgcca
     3121 gaatctcatg agacgatggc gcggcctgag cgtgtgagaa tgtattccgg tgcgacggtc
     3181 cggcatgtac tggaacagat gcgccagggg tggccctctt acggttttcc ggcgctgccg
     3241 catcactggc cggataattt ttatttcagc gacgaccgca ggcccgtagc ctctccgctg
     3301 ccgtcagcgc agcgggtgga tatcaccgct gatgctgtac cggaacaact catgcctgtt
     3361 gtattttcga gagagggaaa cagcaggacg ctgaatctgt tgttatgcaa agggccggag
     3421 gaaatgcttg tcgggtttgt tcgccgggaa ggaggggttt gccccgttct tgtgcttccg
     3481 tcgccggatt acagtcatct gatagtcagc accatcacgg agaacggggt atgcctggca
     3541 ggttacggag aagctataaa ccatgatgag gatactccgt acccaccgga accgcacctg
     3601 atgcagttcc ggctcaaagg ccatcatgac aggcttttgg ctgctgtcca caaaccggaa
     3661 gagatgccgg attatctctt ccgtcaactc ggttttaatc agacctggca tgagtggaag
     3721 cgggacgaac agcacaggca acaacaacgc cgccccgggc acttccgggg catgagtatg
     3781 tgatgtccgg ggctgcaccc cggaccccgc caacacatca cgggccacaa aattttttgt
     3841 ggcccgctct gcgttttcta agtgttatcc ctcctgattt ctaaaaaatt ttccacctga
     3901 gcttgacaga aaaaacgatg acgggtactt tttgatctgt acataaaacc agtggtttta
     3961 tgtacagtat taatcatgta attaattgtt ttaacgctta aaagagggaa tttttatgag
     4021 cggtggcgat ggacgcggcc ataacacggg cgcgcatagc acaagtggta acattaatgg
     4081 tggcccgacc gggcttggtg taggtggtgg tgcttctgat ggttccgggt ggagttcgga
     4141 aaataacccg tggggtggtg gttccggtag cggcattcac tggggtggtg gttccggtca
     4201 tggtaatggc ggggggaata gtaattccgg tggtggctcg ggaacaggcg gtaatctgtc
     4261 agcagtagct gcgccagtgg catttggttt tccggcactt tccactccag gagctggcgg
     4321 tctggcggtc agtatttcag cgggagcatt atcggcagct attgctgata ttatggctgc
     4381 cctgaaagga ccgtttaaat ttggtctttg gggggtggct ttatatggtg tattgccatc
     4441 acaaatagcg aaagatgacc ccaatatgat gtcaaagatt gtgacgtcat tacccgcaga
     4501 tgatattact gaatcacctg tcagttcatt acctctcgat aaggcaacag taaacgtaaa
     4561 tgttcgtgtt gttgatgatg taaaagacga acgacagaat atttcggttg tttcaggtgt
     4621 tccgatgagt gttccggtgg ttgatgcaaa acctaccgaa cgtccaggtg tttttacggc
     4681 atcaattcca ggtgcacctg ttctgaatat ttcagttaat aacagtacgc cagcagtaca
     4741 gacattaagc ccaggtgtta caaataatac tgataaggat gttcgcccgg caggatttac
     4801 tcagggtggt aataccaggg atgcagttat tcgattcccg aaggacagcg gtcataatgc
     4861 cgtatatgtt tcagtgagtg atgttcttag ccctgaccag gtaaaacaac gtcaggatga
     4921 agaaaatcgc cgtcagcagg aatgggatgc tacgcatccg gttgaagcgg ctgagcgaaa
     4981 ttatgaacgc gcgcgtgcag agctgaatca ggcaaatgaa gatgttgcca gaaatcagga
     5041 gcgacaggct aaagctgttc aggtttataa ttcgcgtaaa agcgaacttg atgcagcgaa
     5101 taaaactctt gctgatgcaa tagctgaaat aaaacaattt gatcgatttg cccatgaccc
     5161 aatggctggc ggtcacagaa tgtggcaaat ggccgggctt aaagcccagc gggcgcagac
     5221 ggatgtaaat aataagcagg ctgcatttga tgctgctgca aaagagaagt cagatgctga
     5281 tgctgcatta agtgccgcgc aggagcgccg caaacagaag gaaaataaag aaaaggacgc
     5341 taaggataaa ttagataagg agagtaaacg gaataagcca gggaaggcga caggtaaagg
     5401 taaaccagtt ggtgataaat ggctggatga tgcaggtaaa gattcaggag cgccaattcc
     5461 agatcgcatt gctgataagt tgcgtgataa agaatttaaa aactttgacg atttccggaa
     5521 gaaattctgg gaagaagtgt caaaagatcc cgatcttagt aaacaaggag tcgttatgaa
     5581 aaaaataaca gggattattt tattgcttct tgcagtcatt attctgtctg catgtcaggc
     5641 aaactatatc cgggatgttc agggcgggac cgtatctccg tcatcaacag ctgaagtgac
     5701 cggattagca acgcagtaac ccgaaatcct ctttgacaaa aacaaagcgt gtcagggctg
     5761 attctgatgc gctttttttt tgaaatgtca caaaaattcc atgtgggaga tgggatctaa
     5821 aatcaacgcg ctaaaaacga ttctgacgca ttttttatgt ttaccctggt gtttccttgt
     5881 ctttttgcgt tttttgcgtc agaaagcgat tgagggcgtt ttaagggggc gtacaacggg
     5941 agttatggta aatggatcgg gttttcggga aggatcgaca ggatttgccg ttgggtgtag
     6001 tgtaagcgac tgaaaaacaa acgccccgta aatcgtgctc tcaccgccaa gattgatcac
     6061 gaaattacag ggcgccgggt tccgcgtttc ccgatgggaa agcgcggtta gttaaactgt
     6121 gtaccgagag aaatcgtatc acatgagcgc cgtacttcaa cgcttcaggg aaaaattacc
     6181 gcacaaaccg tactgtacga acgatttcgc gtacggcgtt cgcattctgc cgaaaaacat
     6241 tgccattctt gcccgtttca tccagcagaa ccagccacat gcactgtact ggcttccctt
     6301 tgacgtggac cggatggggg catcaatcga ctggagcgac cggaattcca gctcgttaat
     6361 cagcggcagt attcgcgtca catcagcctg ccgtgtcacc tctgccttca ggaactggag
     6421 taaccaagct cgcaactgca cttcctccgg ctttctgacc tttgccagca atgctgcacc
     6481 cacaatgacc ttctgcgctg tgtccaggct ccggctcttc gccttcaggc gctgtaatct
     6541 ggcctcagct tcggcaatct tctgttcgag tgttctgctc atttcgtgac tccgtgcgcg
     6601 gtgaaaaatc gcattttagc gcgtcactgg tagtttaaaa actgagctgg cataatgcac
     6661 ggcaaatcac gaagtgcgca cttatacaat ctccactccg tttcgattgt gtgcgccctg
     6721 cggggctgaa agaaaactgc aaaaaggcat tacggcagaa atggcgattt atcatctcag
     6781 tatgaaaatc atctccagaa gcaacggcta cagcgccgtt gcttctgctg cctatcgttc
     6841 tggttcacta atgcttgatg agcgtaccgg actgactcac gactacaccc gcaaaagcgg
     6901 tgtggctgag gctgtgattc tgacccccgc gacagcccct gcctggtgta cgaaccgtgc
     6961 cgaactctgg aatgccgttg agaaggccga acgccggaag aactcacaac ttgcgcggga
     7021 aattgaactc gctatccccc gtgaactgcc gcaggatgcc gcacgggaaa ccgttctcgc
     7081 ctttgtcaga gaaaactttg tcagtcaggg catgattgct gatgtggcgt tccatcacat
     7141 ggataaaaca aatccccatg cgcacatcat gctgaccacg agggctgtcg gacctgctgg
     7201 gttcggcgga aaggtcaggg actggaacga ccggacacat gccgagacgt ggcgtgcatc
     7261 atgggctgac catgcaaaca gagcactggc gaacgccgat tatcaggaag agatagacca
     7321 tcgttcatac gagcgacagg ggcttgagaa aacgcctggt attcacctcg gaaagtctgc
     7381 ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc gaacagaatc gtctgattaa
     7441 caggcttaac ctggaaatac agatttcccg cactggatat caccgctgat gctgtaccgg
     7501 aacaactcat gcctgttgta ttttcgagag agggaaacag caggacgctg aatctgttgt
     7561 tatgcaaagg gccggaggaa atgcttgtcg ggtttgttcg ccgggaagga ggggtttgcc
     7621 ccgttcttgt gcttccgtcg ccggattaca gtcatctgat agtcagcacc atcacggaga
     7681 acggggtatg cctggcaggt tacggagaag ctataaacca tgatgaggat actccgtacc
     7741 caccggaacc gcacctgatg cagttccggc tcaaaggcca tcatgacagg cttttggctg
     7801 ctgtccacaa accggaagag atgccggatt atctcttccg tcaactcggt tttaatcaga
     7861 cctggcatga gtggaagcgg gacgaacagc acaggcaaca acaacgccgc cccgggcact
     7921 tccggggcat gagtatgtga tgtccggggc tgcaccccgg accccgccaa cacatcacgg
     7981 gccacaaaat tttttgtggc ccgctctgcg ttttctaagt gttatccctc ctgatttcta
     8041 aaaaattttc cacctgagct tgacagaaaa aacgatgacg ggtacttttt gatctgtaca
     8101 taaaaccagt ggttttatgt acagtattaa tcatgtaatt aattgtttta acgcttaaaa
     8161 gagggaattt ttatgagcgg tggcgatgga cgcggccata acacgggcgc gcatagcaca
     8221 agtggtaaca ttaatggtgg cccgaccggg cttggtgtag gtggtggtgc ttctgatggt
     8281 tccgggtgga gttcggaaaa taacccgtgg ggtggtggtt ccggtagcgg cattcactgg
     8341 ggtggtggtt ccggtcatgg taatggcggg gggaatagta attccggtgg tggctcggga
     8401 acaggcggta atctgtcagc agtagctgcg ccagtggcat ttggttttcc ggcactttcc
     8461 actccaggag ctggcggtct ggcggtcagt atttcagcgg gagcattatc ggcagctatt
     8521 gctgatatta tggctgccct gaaaggaccg tttaaatttg gtctttgggg ggtggcttta
     8581 tatggtgtat tgccatcaca aatagcgaaa gatgacccca atatgatgtc aaagattgtg
     8641 acgtcattac ccgcagatga tattactgaa tcacctgtca gttcattacc tctcgataag
     8701 gcaacagtaa acgtaaatgt tcgtgttgtt gatgatgtaa aagacgaacg acagaatatt
     8761 tcggttgttt caggtgttcc gatgagtgtt ccggtggttg atgcaaaacc taccgaacgt
     8821 ccaggtgttt ttacggcatc aattccaggt gcacctgttc tgaatatttc agttaataac
     8881 agtacgccag cagtacagac attaagccca ggtgttacaa ataatactga taaggatgtt
     8941 cgcccggcag gatttactca gggtggtaat accagggatg cagttattcg attcccgaag
     9001 gacagcggtc ataatgccgt atatgtttca gtgagtgatg ttcttagccc tgaccaggta
     9061 aaacaacgtc aggatgaaga aaatcgccgt cagcaggaat gggatgctac gcatccggtt
     9121 gaagcggctg agcgaaatta tgaacgcgcg cgtgcagagc tgaatcaggc aaatgaagat
     9181 gttgccagaa atcaggagcg acaggctaaa gctgttcagg tttataattc gcgtaaaagc
     9241 gaacttgatg cagcgaataa aactcttgct gatgcaatag ctgaaataaa acaatttgat
     9301 cgatttgccc atgacccaat ggctggcggt cacagaatgt ggcaaatggc cgggcttaaa
     9361 gcccagcggg cgcagacgga tgtaaataat aagcaggctg catttgatgc tgctgcaaaa
     9421 gagaagtcag atgctgatgc tgcattaagt gccgcgcagg agcgccgcaa acagaaggaa
     9481 aataaagaaa aggacgctaa ggataaatta gataaggaga gtaaacggaa taagccaggg
     9541 aaggcgacag gtaaaggtaa accagttggt gataaatggc tggatgatgc aggtaaagat
     9601 tcaggagcgc caattccaga tcgcattgct gataagttgc gtgataaaga atttaaaaac
     9661 tttgacgatt tccggaagaa attctgggaa gaagtgtcaa aagatcccga tcttagtaag
     9721 caatttaaag gcagtaataa gacgaacatt caaaagggaa aagcaccttt tgcaaggaag
     9781 aaagaccaag taggtggtag ggaacgcttt gaattacatc atgataaacc aatcagtcag
     9841 gatggtggtg tctatgatat gaataatatc agagtgacca cacctaagcg acatattgat
     9901 attcatcggg gtaagtaaaa atggaactga aacatagtat tagtgattat accgaggctg
     9961 aatttctgga gtttgtaaaa aaaatatgta gagctgaagg tgctactgaa gaggatgaca
    10021 ataaattagt gagagagttt gagcgattaa ctgagcaccc agatggttca gatctgattt
    10081 attatcctcg cgatgacagg gaagatagtc ctgaagggat tgtcaaggaa attaaagaat
    10141 ggcgagctgc taacggtaag tcaggattta aacagggctg aaatatgaat gccggttgtt
    10201 tatggatgaa tggctggcat tctttcacaa caaggagtcg ttatgaaaaa aataacaggg
    10261 attattttat tgcttcttgc agtcattatt ctgtctgcat gtcaggcaaa ctatatccgg
    10321 gatgttcagg gcgggaccgt atctccgtca tcaacagctg aagtgaccgg attagcaacg
    10381 cagtaacccg aaatcctctt tgacaaaaac aaagcgtgtc aggctgattc tgatgcgctt
    10441 tttttttgaa atgtcacaaa aattccatgt gggagatggg atctaaaatc cccgtgcaga
    10501 actttccatc cagggggaga aaacttgtcg ttttgagccg ttcggtgttc agaacgcacg
    10561 aaaccgatcg cgcatcgctt tcgtgcatag ttatgcaacg cgctaaaaac gattctgacg
    10621 cattttttat gtttaccctg gtgtttcctt gtctttttgc gttttttgcg tcagaaagcg
    10681 attgagggcg ttttaagggg ggcgtacaac gggagttatg gtaaatggat cgggttttcg
    10741 ggaaggatcg acaggatttg ccgttgggtg tagtgtaagc gactgaaaaa caaacgcccc
    10801 gtaaatcgtg ctctcaccgc caagattgat cacgaaatta cagggcgccg ggttccgcgt
    10861 ttcccgatgg gaaagcgcgg ttagttaaac tgtgtaccga gagaaatcgt atcac
//
