LOCUS       NZ_AP027184             6676 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain 98E11 plasmid pO26_98E11_3, complete
            sequence.
ACCESSION   NZ_AP027184
VERSION     NZ_AP027184.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562886
            Sequence Read Archive: DRR424514
            Assembly: GCF_027925765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan:Fukuoka
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 95x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925765.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:27:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,160
            CDSs (total)                      :: 6,020
            Genes (coding)                    :: 5,693
            CDSs (with protein)               :: 5,693
            Genes (RNA)                       :: 140
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 327
            CDSs (without protein)            :: 327
            Pseudo Genes (ambiguous residues) :: 0 of 327
            Pseudo Genes (frameshifted)       :: 118 of 327
            Pseudo Genes (incomplete)         :: 205 of 327
            Pseudo Genes (internal stop)      :: 78 of 327
            Pseudo Genes (multiple problems)  :: 65 of 327
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027184.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="98E11"
                     /serovar="O26:H11"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO26_98E11_3"
                     /geo_loc_name="Japan:Fukuoka"
                     /collection_date="1998"
     gene            complement(6..197)
                     /locus_tag="QMG82_RS30665"
     CDS             complement(6..197)
                     /locus_tag="QMG82_RS30665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            265..588
                     /locus_tag="QMG82_RS30670"
                     /old_locus_tag="F98E11_p3_01"
     CDS             265..588
                     /locus_tag="QMG82_RS30670"
                     /old_locus_tag="F98E11_p3_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            797..>1231
                     /locus_tag="QMG82_RS30675"
                     /pseudo=""
     CDS             797..>1231
                     /locus_tag="QMG82_RS30675"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1284..1826
                     /locus_tag="QMG82_RS30680"
     CDS             1284..1826
                     /locus_tag="QMG82_RS30680"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_100029810.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1833..2066
                     /locus_tag="QMG82_RS30685"
     CDS             1833..2066
                     /locus_tag="QMG82_RS30685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001345152.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2557..2802)
                     /locus_tag="QMG82_RS30690"
                     /old_locus_tag="F98E11_p3_04"
     CDS             complement(2557..2802)
                     /locus_tag="QMG82_RS30690"
                     /old_locus_tag="F98E11_p3_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3066..5159
                     /gene="cda"
                     /locus_tag="QMG82_RS30695"
                     /old_locus_tag="F98E11_p3_05"
     CDS             3066..5159
                     /gene="cda"
                     /locus_tag="QMG82_RS30695"
                     /old_locus_tag="F98E11_p3_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            5156..5419
                     /locus_tag="QMG82_RS30700"
                     /old_locus_tag="F98E11_p3_06"
     CDS             5156..5419
                     /locus_tag="QMG82_RS30700"
                     /old_locus_tag="F98E11_p3_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            5489..5635
                     /locus_tag="QMG82_RS30705"
                     /old_locus_tag="F98E11_p3_07"
     CDS             5489..5635
                     /locus_tag="QMG82_RS30705"
                     /old_locus_tag="F98E11_p3_07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            6342..6530
                     /locus_tag="QMG82_RS30710"
                     /old_locus_tag="F98E11_p3_09"
     CDS             6342..6530
                     /locus_tag="QMG82_RS30710"
                     /old_locus_tag="F98E11_p3_09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244575693.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDVHSLLTHLCG
                     ISHPAWRTFHTIRTVAA"
                     /name="hypothetical protein"
     BGC             1..6676
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6510..6594
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          5865..6049
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          579..2173
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
       61 tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt cgtcggcgtc cagttcattc
      121 agtttttcca gcagggtcag cgtctggctt ctgatgaagc gggccatgtt aagggcggtt
      181 ttttcctgtt tagtcacagt tctgtttctc catgtgtggg gagtctgtcg gaggttttta
      241 atgataccgg tgaaacgcga aaggatgctc acgatacggg tcacggatga ggaacatgcg
      301 cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa ggtctgcctg
      361 ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg caccgccgct gttgcgccag
      421 ttcgcttcgg tcggcaataa cctgaatcag atagcccgga agataaacag tggacagtgg
      481 tcggggcatg accgggttca tgtggtggct gcactgatgg ccatcggacg tgaactgagt
      541 gagctgaggg atgaggtccg taaacaggga gaacgcgatg atagttaagt ttcatgccag
      601 gggaaaaggc ggtggcagtg gtccggttga ttacctgctg ggccgggagc gtaaccggga
      661 aggggcgaga gttctgcggg gtgctcccga agaggtgcgg gaactcatcg atgccacgcc
      721 gtttgcgaaa aaatacacct cgggggttct gtcttttgca gagcagacac tgccaccggg
      781 agagcgtgaa agggtgatgg agagctttga gcgggtactg atgcccggtc ttgaaaaaaa
      841 tcagtacagc atcctgtggg tggagcacca ggacaagggg cgtcttgagc tgaattttgt
      901 catcccgaat atggagctgg cgagcggaaa acggctccag ccctattacg accgggcgga
      961 ccgtccgcga atcaatgcct ggcagacact ggtgaatcac cattacgggc tgcacgaccc
     1021 gaacgcaccg gagaacaggc ggacacttgt caccccgaat aatctgccga aagcgaaaca
     1081 ggaggccgca gaagcgatta cgaggggact ggaggctctt taccatgccg gagcgctaaa
     1141 aacgcgtcag gatgtcactg aggcgcttac agcggcaggt tttgaggtgg tgaggacaac
     1201 caggagcagt atcagtatcg ccgacccgga gggcgggcgt aatctcaggc tgaaaggggc
     1261 gctgtatgag caatctttta caaatgggga cagacttcga gaagaaactg aaagagcgtg
     1321 ccgtctctac agagaacatg ctgaacagcg agttcaggaa gctcgaagcg tctgtagaca
     1381 gggaactgaa cttaaacggg cagaaaatca gagacgctat cagcgcacac acagcagcgg
     1441 tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc gcagttcagc gccacagaga
     1501 acgaacttgc ccggcggcag gaagagctgc tctggaagct ggtcaaaggg cggatactgt
     1561 acccgtcgct gacggcgctt tgcgtcacgg tcgggatttt tctggcgtca tgggggctga
     1621 ttcagtggca ggaaagccgt attgcggcga acatcctggc tatcagggac caggaagaga
     1681 cgctggcgaa actccgggaa aaaacatggg gtgtgacgta tcacgagggc aggaacggga
     1741 aatttctggt gttgccgtcc ggggtgaagg gggaaaacaa ctggacggtg gagaagaaga
     1801 acgcagtaag actggtcagg gagtaagtga gaatgacaga gctggaaatg cacttactga
     1861 acgccttaga gcaattgcag caggactata cgcagcggct gaaagactgg gagagcgcct
     1921 tcacggattt gcagcatatg ttcgagctga cacgacagga gaacgggcaa ctgagggaac
     1981 agtgcgacgg cttgagtcag caggtgcaga gattagccgg gcaggtggac cgcttgagtc
     2041 ggttatttat cacgaacagc agataaggga tgaacaggaa aggctggcac gcctggaacg
     2101 ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa gtacgggtat ggcgtggccc
     2161 gtcaatgggg atgtgacggt gagttgttcc gggaggttgc cagatggtac cggaacgcgt
     2221 taaaaacgca tccgggcgcg attctggcgt gtcgtttttg ttgctgtgtt ttgcctcttt
     2281 gccagatcgc gctcattcct cagcctcatc ccgcgatgtc tgcgtatgtc atcaactgaa
     2341 aagatggaaa aaaaacagtg tcatgaggtt tacgcaggca aaaccagcgt tattcacatg
     2401 gctgaatagc tatgcatacg gttaaaattt aatcaggtgc gatcgcggca gcttttcggg
     2461 tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc gctgaacgca tttcagcggt
     2521 gcgtacaata aggggttatg ttaaataacc cttagcttag cggcggcgca gtggttgcca
     2581 ggtgaaggtg tcatccgggt cctgtccggc agtcagtgtg gcttctcgat ttttgctggc
     2641 cgttcgttca tcaagaatgg ctttaagtat ccgtgcccgg aggcgtcgct ttgtttcctg
     2701 cggcattttg tcctttttcg tcagccccat ggcacgagct tcagcggcaa tggcttctgc
     2761 cagtcggcgc tcttcggctt cggtcagtct atggcgtttc atggtggtct ccgggtgttt
     2821 ctctggtgtc ataacagttt aactgttggc tctctcctta tgctaatcag ccttctttca
     2881 tgcctgttgc cattgagttt aagaatgatt ggttgttttt cttgagcgtt ttcacaaaaa
     2941 cgtaaatcca gtgggttgac aggatttacg aggtgacgta actttttact gtacataaaa
     3001 ccagtggttt tatgtacagt ttgttgtttt taacgcattg ttttaaaagt caaagaggtg
     3061 tttttatgag tgattacgaa ggtagtggtc cgacagaagg tattgattat gggcactcga
     3121 tggtcgtgtg gccgtcaaca ggactgattt cgggtggtga tgtgaaacca ggaggctcat
     3181 caggtatcgc tccatccatg cctccgggat ggggggatta cagcccacag ggtatcgcac
     3241 ttgtacaaag tgttcttttt ccgggaatta ttcgccggat tattctggat aaggaacttg
     3301 aagagggaga ctggtcggga tggtctgtca gtgtgcatag cccttgggga aacgagaaag
     3361 tttccgctgc acgaacagtt cttgagaatg gtttacgtgg tggtttgcca gaaccgtctc
     3421 gcccggctgc tgtttctttt gcccgtctgg agcctgcttc cggaaatgag caaaaaatta
     3481 ttcgtcttat ggttacacag caactggaac aggtaacgga tattccggcc agccagttac
     3541 cagcagcggg taataatgta ccggtgaaat atcgtctgat ggaccttatg cagaacggta
     3601 cgcaatatat ggctattatc ggaggtattc cgatgacagt gccggtagtt gatgccgttc
     3661 cagttccgga ccggagtcgt ccgggaacaa atattaaaga tgtttacagt gcccctgtat
     3721 caccaaatct accggacctg gtattaagtg tgggtcagat gaatactccg gttctgtcta
     3781 atcccgaaat tcaggaagaa ggagttattg ctgagacagg taattatgtt gaggctggtt
     3841 atacgatgtc cagtaataat catgatgtca ttgttcgttt tcctgatggc agtggtgttt
     3901 ctccgttata tatttcaact gtagagattc tggacagtaa tggtctgagt cagcgtcagg
     3961 aagccgaaaa taaagcaaag gatgatttca gagtcaagaa agaagaagcg gtagcccggg
     4021 ctgaagctga aaaagcaaaa gccgagttat ttagtaaagc aggtgtgaac cagcctcctg
     4081 tatatacaca ggaaatgatg gaaagggcta attcagtaat gaatgagcag ggagctcttg
     4141 ttctgaataa cactgccagt tctgtacaac tggcgatgac cggaactggt gtctggactg
     4201 ctgctggtga tattgcaggc aacataagca agtttttcag taatgctctg gagaaagtaa
     4261 ccatacccga agtgagtccc ctgcttatgc ggatttctct tggtgctctg tggtttcatt
     4321 cagaagaagc tggagcagga agtgatatcg tgccggggcg aaatctggag gcgatgtttt
     4381 cgctgagtgc tcagatgttg gctggacagg gcgtggtcat tgaacctggt gcgacgagtg
     4441 taaatctgcc tgttcgtgga caattgataa acagtaacgg gcaattagct ctggatttac
     4501 tgaaaacagg gaatgaaagt atcccagctg cggttcctgt tcttaatgct gtccgtgata
     4561 cagcaacagg actggataaa atcacgttac cagcagtagt aggcgcacct tcccggacga
     4621 ttctggttaa tccggtacca caaccttcgg tgccaacaga tacaggtaat catcaacccg
     4681 ttccggttac accagtgcat acaggaacgg aagtaaaacc ggtcgaaatg ccagtaacga
     4741 cgattactcc tgtttctgat gttggtggac tacgagattt tatttactgg cgtcctgatg
     4801 ctgctgggac tggtgttgaa gctgtttatg tgatgcttaa tgatcctctg gattcagggc
     4861 gattttcgcg taaacaactt gataaaaaat ataaacatgc tggtgatttt ggtattagtg
     4921 atacaaaaaa gaatcgtgaa actcttacta aattcaggga tgctattgag gagcatttat
     4981 cggataagga tacagtagag aaaggaacat accgaagaga aaaaggttca aaagtttatt
     5041 ttaatcctaa tacgatgaat gtggttataa ttaagtcaaa tggtgagttc ttatctgggt
     5101 ggaaaataaa tccagatgcg gataatggtc gaatttattt agagacaggt gaactatgaa
     5161 caagatggca atgatcgatt tggcgaaatt atttttagct tcgaaaatta cagcaattga
     5221 gttttcagag cgaatttgtg ttgaacggag aagattgtat ggtgttaagg atttgtctcc
     5281 gaatatatta aattgtgggg aagagttgtt tatggctgct gagcgatttg agcctgatgc
     5341 agatagggct gattatgaaa ttgatgataa tggacttaag gttgaggtcc gatctatctt
     5401 ggaaaaattt aaattataat catgagtgat tttaatatct ggcatttaag tataaatgtc
     5461 agatattcac tcatgacaag gagatgtcat gaaaaaaaag atagggggat ctatgattct
     5521 cgttcttgct gttttgtgtc tgactgcttg ccaagctaac tatgtaaggg atgttcaggg
     5581 tggaacaatt gctccatcat catcttcgaa actaatcggg gtggcggttc agtgaaaaat
     5641 attaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgcct gtaaacgaaa
     5701 aaacccgcct tgtggcgggt ccttccgtcc gatcagaagt cttcgacctc ttctaatcgg
     5761 cggtggtaac tggcttggag gagcacagtc accaaaactg tcttttcagt ttagccttaa
     5821 ctggttcata acttcaagag caactcctct aaatcagtta ccaatggctg ctgccagtgg
     5881 cgttaagtcg tgtcttttcc gggttggccc caagagaaca gttaccggag aaggtgcagc
     5941 agtcgggctg aacggggggt tcctgcatac agcccagccc ggagcgaact gcctaagtcg
     6001 ccccgagata ccagaagacg tgagctatga gaaagcgcca cgtgtcccgc aagggagaaa
     6061 ggcggaaaag gtatccggta atcgatatgg cgggaacgtg ggggcgcagg agggagcgat
     6121 tcaccggaaa cggtgatgat ctttaagcca cgtcgggttt cgccaccgct ggcggatttc
     6181 atggttgagc ctcacggctc ccacagatgc accgaaaaag tgtctgtttt gtgaagtccg
     6241 tcaggggggc ggagcctatg gaaaaagcgc ctccggcgcg gcctttttcg gggctttcgt
     6301 ttcttgctca catgttcttt ccggcgttat cccctgattc tgtggataac actatcaccg
     6361 cttttgagtg ggcagatacc gctcgccgca gccgaacgac cgagcggagc gagtcagtga
     6421 gcgaggaagc ggaaaagcgc ctggacgtgc attctctcct tacgcatctg tgcggcattt
     6481 cacacccggc atggcgcact tttcatacaa tccgcactgt tgccgcataa ttaagccagt
     6541 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgctaaaacc
     6601 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gctcacaggc aagctgtgac
     6661 cgtctccggg agctgc
//
