LOCUS       NZ_AP027180             7324 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain EH2252 plasmid pO26_EH2252_4, complete
            sequence.
ACCESSION   NZ_AP027180
VERSION     NZ_AP027180.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562885
            Sequence Read Archive: DRR424513
            Assembly: GCF_027925745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 70x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925745.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:14:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,303
            CDSs (total)                      :: 6,170
            Genes (coding)                    :: 5,795
            CDSs (with protein)               :: 5,795
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 147 of 375
            Pseudo Genes (incomplete)         :: 235 of 375
            Pseudo Genes (internal stop)      :: 84 of 375
            Pseudo Genes (multiple problems)  :: 80 of 375
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027180.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EH2252"
                     /serovar="O26:H11"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO26_EH2252_4"
                     /geo_loc_name="Belgium"
                     /collection_date="2013"
     gene            complement(6..197)
                     /locus_tag="QMG71_RS31395"
     CDS             complement(6..197)
                     /locus_tag="QMG71_RS31395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165978.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            264..587
                     /locus_tag="QMG71_RS31400"
     CDS             264..587
                     /locus_tag="QMG71_RS31400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024177064.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            796..>1230
                     /locus_tag="QMG71_RS31405"
                     /pseudo=""
     CDS             796..>1230
                     /locus_tag="QMG71_RS31405"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1283..1825
                     /locus_tag="QMG71_RS31410"
     CDS             1283..1825
                     /locus_tag="QMG71_RS31410"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_231200040.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1832..2065
                     /locus_tag="QMG71_RS31415"
     CDS             1832..2065
                     /locus_tag="QMG71_RS31415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2550..2969)
                     /locus_tag="QMG71_RS31420"
                     /old_locus_tag="EH2252_p4_04"
     CDS             complement(2550..2969)
                     /locus_tag="QMG71_RS31420"
                     /old_locus_tag="EH2252_p4_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3053..3490)
                     /locus_tag="QMG71_RS31425"
                     /old_locus_tag="EH2252_p4_05"
     CDS             complement(3053..3490)
                     /locus_tag="QMG71_RS31425"
                     /old_locus_tag="EH2252_p4_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177063.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRR
                     EFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            3748..5841
                     /gene="cda"
                     /locus_tag="QMG71_RS31430"
                     /old_locus_tag="EH2252_p4_06"
     CDS             3748..5841
                     /gene="cda"
                     /locus_tag="QMG71_RS31430"
                     /old_locus_tag="EH2252_p4_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_062859458.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPAAGN
                     NVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKVELFSKAGVKQPPVY
                     TQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGMVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDAGGLRDFIYWR
                     PDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            5838..6101
                     /locus_tag="QMG71_RS31435"
                     /old_locus_tag="EH2252_p4_07"
     CDS             5838..6101
                     /locus_tag="QMG71_RS31435"
                     /old_locus_tag="EH2252_p4_07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038406.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDVL
                     NCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            6171..6317
                     /locus_tag="QMG71_RS31440"
                     /old_locus_tag="EH2252_p4_08"
     CDS             6171..6317
                     /locus_tag="QMG71_RS31440"
                     /old_locus_tag="EH2252_p4_08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071527535.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTGV
                     AIQ"
                     /name="colicin release lysis protein"
     gene            complement(6546..6659)
                     /locus_tag="QMG71_RS31560"
     CDS             complement(6546..6659)
                     /locus_tag="QMG71_RS31560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001428439.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
                     /name="hypothetical protein"
     BGC             1..7324
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7158..7245
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(6588..7135)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.011"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          578..2172
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.308"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
       61 tcggcgtggt cgtgaagggc ttcgcagatg tctgcctggt cgtcggcgtc cagttcattc
      121 agtttttcca gcaggatcag cgtctggctt ctgataaagc gggccatgtt aagggctgtt
      181 ttttcctgtt tagtcacagt tctgttctcc atgtgtgggg agtctgtcgg aggtttttaa
      241 tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag gaacatgcgc
      301 ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg
      361 gtgcaccgcc atcgaaaacg tcaggtcttc cgacactggc accgccgctg ttgcgccagt
      421 tcgcttcggt cggcaataac ctgaatcaga tagcccggaa gataaacagc ggacactggt
      481 cggggcatga ccgggttcat gtggtggctg cactgatggc catcggacgt gaactgagtg
      541 agctgaggga tgaggtccgt aaacagggag aacgggatga tagttaagtt tcatgccagg
      601 ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg gccgggagcg taaccgggaa
      661 ggggcgagaa ttctgcgggg tgatcccgaa gaggtgcggg aactcatcga tgccacgccg
      721 tttgcgaaaa aatacacctc gggggttctg tcttttgcag agcagacact gccaccggga
      781 gagcgtgaaa gggtgatgga gagctttgag cgggtactga tgcccggtct tgaaaaaaat
      841 cagtacagca tcctgtgggt ggagcaccag gacaaggggc gtcttgagct gaattttgtc
      901 atcccgaata tggagctggt gagcggaaaa cggctccagc cctattacga ccgtgcggac
      961 cgtccgcgaa tcaatgcctg gcagacactg gtgaatcatc attacgggct gcacgacccg
     1021 aacgcaccgg agaacaggcg gacacttgtc accccgaata atctgccgaa agcgaaacag
     1081 gaggccgcag aagcgattac gagggggctg gaggctcttt atcatgccgg agcgctaaaa
     1141 acgcgtcagg atgtcactga ggcgcttaca gcggcaggtt ttgaggtggt gaggacaacc
     1201 aggagcagta tcagtatcgc cgacccggag ggcgggcgta atctcaggct gaaaggggcg
     1261 ctgtatgagc aatcttttac aaatggggac agacttcgag aagaaactga aagagcgtgc
     1321 cgtctctaca gagaacatgc tgaacagcga gttcaggaag ctcgaagcgt ctgtagacag
     1381 ggaactgaac ttaaacgggc agaaaatcag agacgctatc agcgcacaca cagcagcgct
     1441 gaaagaacaa ctggagacgc tgaacagtgc cgtgaacgcg cagttcagcg ccacagagaa
     1501 cgaactggcc cggcggcagg aagagctgct ctggaagctg atcaaagggc ggatactgta
     1561 cccgtcgctg acggcgcttt gcgtcacggg cgggattttt ctggcgtcat gggggctgat
     1621 tcagtggcag gaaagccgga ttgcggcgaa catcctggct atcagggacc aggaagagac
     1681 gctggcgaaa ctccgggaaa aaacatgggg tgtgacgtat cacgagggca ggaacgggaa
     1741 atttctggta ttgccgtccg gggtgaaggg ggaaaacaac tggacggtgg agaagaagaa
     1801 cgcagtaaga ctggtcaggg agtaagtgag tatgacagag ctggaaatgc acttactgaa
     1861 cgccttagag caattgcagc aggactatac gcagcggctg aaagactggg agagcgcctt
     1921 cacggatttg cagcatatgt tcgagctgac acgacaggag aacgggcaac tgagggaaca
     1981 gtgcgacgac ttgagtcagc aggtgcagag attagccggg caggtggacc gcttgagtcg
     2041 gttatttatc acgaacagca gataagggat gaacaggaaa ggctggcacg cctggaacgg
     2101 gagcgtgcgc ggtcctggca tgaagagcag gaacgggaag tacgggtatg gcgtggcccg
     2161 tcaatgggga tgtgacggtg agttgttccg ggaggttgcc agatggtacc ggaacgcgtt
     2221 aaaaatgcat ccgggcgcga ttctggcgtg tcgtttttgt tgctgtgttt tgcctctttg
     2281 ccagatcgcg ctcattcctc agcctcatcc cgcgatgtct gcgtatgtca tcaactgaaa
     2341 agatggaaaa aaaacagtgt catgaggttt acgcaggcaa aaccagcgtt attcacatgg
     2401 ctgaatagct atgcatacgg ttaaaattta tcaggtgcga tcgcggcagc ttttcgggtg
     2461 gtttgttgcc atttttacct gtcagcagcc gtgatcgcgc tgaacgcatt tcagcggtgc
     2521 gtacaattaa gggattatgg taaatccact tactgtcttc cctcgtagcc atcgagataa
     2581 accgcagaaa tcgtctcagc cagcagcctg gattttttcg gggtaaggtc tttgctgcgg
     2641 taatcagtac ccgcaaattg agttgtgccc cggagtgagt ttaaaaattc ctggctgtga
     2701 aactgctgaa tgcgttgctg cacatcactc cgtgattttc ctgtcgcttt atcctttgct
     2761 cccatatccc agaactgccg gaaaaaaggc accatcattc gtgttgtgtc ggccttctgg
     2821 gtggaaaagt taggatcaaa accatcgtct gatgcataaa cgtaatgccg ggtatgttgt
     2881 tccacactgc cctttggcgt gcaggctgtc aggaccgttg ccgctgtcag cagggcgata
     2941 agatacagtg tgccgccgga acgtctcatg agtgtctcct tacctttaaa tctttgtact
     3001 cagtgactta tatttaaccg ctgactatct tatcatcact tgcagccgat gctcaccggc
     3061 tgaacagtct ggcccagaat ccccgttttt tttcctgctc cagtgcctgc ctgagctgct
     3121 ctatctcttt atgtagctgt tcccgttcgg cttcctggcg ccggcgttcc gtatcctgtg
     3181 cctgtttgtc ctcaagcatc agtgtcaggc attgtttcag ctcattcagc tcccggagaa
     3241 tgcgttctgt ctgctgatca tgtggatttt ctgcctgtcc cgcactgtgc ctttctgctg
     3301 tctcatcctg ctttaattcg ccgtatgccc ggatcagttc actggtttca aactcccgtc
     3361 gtccgtcctg gccggtacgg taactgacaa ggcctgcgcg catatcacgg taaaactgtg
     3421 aacgcgatct gcctgtcatt ttcagtgcgt cctggagtgt gtgccatgcc atgaagtgac
     3481 agtgtcccat tggtgtctca tatcatggtt tcagtaaaac atactggggc cggagaacgg
     3541 taatgtttgt gctggttttt gtggcatcgg gtgggaatag cacgtggtgt gggtggctgt
     3601 ttttttgatc gttttcacaa aaacgaaaat ccacagggtt gacagggaaa acgcagcggc
     3661 gtaattttaa actgtatata aaaccagtgg ttgtatatac agtatgcgtc ttttaactat
     3721 ttgtttttaa tgtcaaagag gttttctatg agtgataatg aaggtagtgt cccgacagaa
     3781 ggtattgatt acggggacac aatggttgtg tggccgtcaa ctggacgaat tccgggcggt
     3841 gatgtgaaac ccggtggttc atcaggtctc gctccatcca tgcctccggg atggggagat
     3901 tacagtccac aaggtatcgc acttgtacaa agtgttcttt ttccaggaat tattcgccgg
     3961 attatccttg ataaggaact tgaagagggg gactggtcgg gatggtctgt cagtgtgcat
     4021 agtccctggg gaaacgagaa agtttctgct gcacgaacag ttcttgagaa tggtttacgt
     4081 agtggtttgc cagaaccatc acgcccggct gctgtttctt ttgcccgtct ggagcctgct
     4141 tccggaaatg agcaaaaaat tattcgtctt atggttacac agcaactgga gcaggtaacg
     4201 gatattcctg tcagccagtt accagcagcg ggtaataatg taccggtgaa atatcgtctg
     4261 acggacctta tgcagaacgg tacgcaatat attgctatta tcggaggtat tccgatgaca
     4321 gtgccggtag ttgatgccgt tccggttccg gaccggagcc gtccgggaac aaatattaaa
     4381 gatgtttaca gtgcccctgt atcaccaaat ctaccggacc tggtattaag tgtggggcag
     4441 atgaatactc cggttctgtc taatcccgaa attcaggaag agggcgttat tgctgagaca
     4501 ggtaattatg ttgaggcagg ttatacgatg tccagtaata atcatgatgt cattgttcgt
     4561 tttcctgacg gcagcggtgt ttctccgtta tatatttcaa ctgtggagat tcttgaccgt
     4621 aatggtctga gtcagcgtca ggaagcagaa aataaagcaa aggatgattt cagagtcaag
     4681 aaagaagaag cggtagcccg ggccgaagct gaaaaagcaa aagtcgagtt atttagtaaa
     4741 gcaggtgtga aacagcctcc tgtatataca caggaaatgg tggaaagggc taattcagta
     4801 atgaatgagc agggaactct tgttctgaat aacactgcca gttctgtaca actggcgatg
     4861 accggaactg gtgtctggac ggctgctggt gatattgcag gcaacataag caagtttttc
     4921 agtaatgctc tggagaaagt aaccataccc gaagtgggtc ccctgcttat gcggatttct
     4981 cttggcgctc tgtggttcca ttcagaagac gccggagcag gaagtgatat cgtgccgggg
     5041 cgagatatgg aggctatgtt ctcgctgaat gctcagatgt tggcaggaca gggcatggtc
     5101 attgaacctg gtgctacgag tgttaatctg cctgttcgtg gacaattgat aaacagtaac
     5161 ggacaacttg ctctggattt actgaagaca gggaatgata gtatccctgc tgcggttcct
     5221 gttcttaatg ctgttcgtga tacagctaca ggactggata aaattacgct gccagcagta
     5281 gtcggtgcac cttccaggac gattctggta aatccggccc cacaaccttc ggcaccaaca
     5341 gatacaggaa atcatcaacc cgttccggtt accccagtgc atacaggtac ggaggtaaaa
     5401 ccggttgaaa tgccggtaac gacgattact ccagtctctg atgccggtgg acttcgagat
     5461 tttatttact ggcgtcctga tgctaccggg actggtgttg aagctgtcta tgtgatgctt
     5521 aatgatcctc tggattcagg gcggttttca cgcaaacagc ttgataaaaa atataaacat
     5581 gctggtgatt ttggtattag tgatactaaa aagaatcgtg aaactcttac taaattcagg
     5641 gatgctattg aggaacattt atccgataag gatacagtag agaaaggaac ataccgacga
     5701 gaaaaaggat caaaagttta ttttaatcct aatacaaata atgtggtgat actaaaatca
     5761 aatggtgagt tcttatctgg gtggaaaata aatccagatg cggataatgg gcgaatttat
     5821 ttagaaacag gtgaactatg aacaagatgg cgataatcga tttggcgaag tcatttttag
     5881 cttcaaaaat aacagcaatg gagttttcag agcggatttg tgttgagcgg agaaaattgt
     5941 atggtgttaa gaatttgtct caggatgtat tgaattgtgg ggaggagttg tttatagctg
     6001 ctgagcgttt cgagcctgat tctgataggg ctgattatga attcgatgat aatggactta
     6061 ggattgaagt ccaatctatc ctggaaaaat ttaaattata aatatgagtg attttaatat
     6121 ctggaattta attataaatg tcagatactc actcatgaca aggagatgtt atgaaaaaaa
     6181 agataggggt atcgattatt ctcgttcttg ttgttttgtg tctgactgct tgtcaaaata
     6241 actatgtaag ggatgttcag ggaggaacga ttgctccatc atcatcctcg aaactaaccg
     6301 gggtagcgat tcagtgaaaa agattaaagg atcttcttga gatccttttt ttctgcgcgt
     6361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccaacg gtggtttgtt tgccggaaca
     6421 agagctacca actctttttc cgaaggtaac tggctttagt ggagcacaga taccaaatgc
     6481 tgtcctttca gtgtagccgc gataaggctt caacttcaag agttctgcgt atctaaatcc
     6541 acttttcagt taccaatggc tgctgccagt ggcgtaaagt cgtgtcttac cgggttggac
     6601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6661 cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga
     6721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6781 ggaacaggag agcgcacgag ggagcttccg gggggaaacg cctggtatct ttatagtcct
     6841 gtcgggtttc gccacctctg gcttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6901 agcctatgga aaaagcgcca ccggcgcggc cttttccccg gctttgtctt gttgctcaca
     6961 tgttctttcc ggctttatcc cctgattctg tggataaccg tattaccgct tttgagtgag
     7021 ctgacaccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     7081 aagagcatcg gcatctgcat gttctcctta cgcatctgtg cggcatttca caccgtacat
     7141 ggtgcacttt tcatacaatc ctcactgatg ccacacgatt aagccagtat acactccgct
     7201 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ctaaaacccg ctgacgcgcc
     7261 ctgacgggct tgtctgctcc cggcatccgc tcacaggcaa gctgtgaccg tctccgggag
     7321 ctgc
//
