LOCUS       NZ_AP027173             6758 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain 20.1 plasmid pO26_20.1_3, complete sequence.
ACCESSION   NZ_AP027173
VERSION     NZ_AP027173.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562884
            Sequence Read Archive: DRR424512
            Assembly: GCF_027925685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 74x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925685.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:12:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,055
            CDSs (total)                      :: 5,925
            Genes (coding)                    :: 5,573
            CDSs (with protein)               :: 5,573
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 352
            CDSs (without protein)            :: 352
            Pseudo Genes (ambiguous residues) :: 0 of 352
            Pseudo Genes (frameshifted)       :: 141 of 352
            Pseudo Genes (incomplete)         :: 215 of 352
            Pseudo Genes (internal stop)      :: 80 of 352
            Pseudo Genes (multiple problems)  :: 74 of 352
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027173.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="20.1"
                     /serovar="O26:H11"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pO26_20.1_3"
                     /geo_loc_name="Belgium"
                     /collection_date="2008"
     gene            1..894
                     /locus_tag="QMG87_RS30075"
                     /old_locus_tag="B201_p3_01"
     CDS             1..894
                     /locus_tag="QMG87_RS30075"
                     /old_locus_tag="B201_p3_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277131.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(1110..1385)
                     /locus_tag="QMG87_RS30080"
                     /old_locus_tag="B201_p3_02"
     CDS             complement(1110..1385)
                     /locus_tag="QMG87_RS30080"
                     /old_locus_tag="B201_p3_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            1564..3783
                     /gene="mobQ"
                     /locus_tag="QMG87_RS30085"
                     /old_locus_tag="B201_p3_03"
     CDS             1564..3783
                     /gene="mobQ"
                     /locus_tag="QMG87_RS30085"
                     /old_locus_tag="B201_p3_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029327.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAQRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4016..5761
                     /gene="col"
                     /locus_tag="QMG87_RS30090"
                     /old_locus_tag="B201_p3_04"
     CDS             4016..5761
                     /gene="col"
                     /locus_tag="QMG87_RS30090"
                     /old_locus_tag="B201_p3_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_001347430.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQ
                     FKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHID
                     IHRGK"
                     /name="col"
     gene            5764..6024
                     /locus_tag="QMG87_RS30095"
                     /old_locus_tag="B201_p3_05"
     CDS             5764..6024
                     /locus_tag="QMG87_RS30095"
                     /old_locus_tag="B201_p3_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6086..6229
                     /locus_tag="QMG87_RS30100"
                     /old_locus_tag="B201_p3_06"
     CDS             6086..6229
                     /locus_tag="QMG87_RS30100"
                     /old_locus_tag="B201_p3_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6758
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1390..1563)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1564..1922)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..894
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.387"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1565..3780
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.865"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg tacttcaacg cttcagggaa aaattaccgc acaaaccgta ctgtacgaac
       61 gatttcgcgt acggcgttcg cattctgccg aaaaacattg ccattcttgc ccgtttcatc
      121 cagcagaacc agccacatgc actgtactgg cttccctttg acgtggaccg gatgggggca
      181 tcaatcgact ggagcgaccg gaattgtccg gccccgaaca tcaccgtaaa aaatccccgt
      241 aacgggcacg cgcatctgct ctacgcgctc gcccttcctg tgagaactgc gccggatgca
      301 tcggcttcgg cgctcagata cgctgccgct attgagcgtg cgttgtgtga aaaactgggc
      361 gcggatgtga attacagcgg cctgatctgc aaaaatccgt gccaccctga atggcaggaa
      421 gtggaatggc gcgaggaacc ctacactctc gacgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gccgtagcgt cgataaaaat tacgggctgg ggcgaaacta ccatctgttc
      541 gaaaaggtcc gtaaatgggc ctacagagcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tctccggctg aatgtcgggc tattggcaag agcattgcga aatatacaca caggaaattc
      721 tcaccagagg gattttccgc tgtacaggcc gctcgaggtc gcaagggcgg aactaaatct
      781 aagcgcgcag cagttcctac atcagcacgt tcgctgaagc cgtgggaggc attagggatc
      841 agtcgagcga cgtactaccg aaaattaaaa tgtgatccag atctcgcaaa atgagaccag
      901 ataagcctta tcagataaca gcgccctttt ggcgtctttt tgagcagtga tctttcttgc
      961 gattttctcc gttctgcccc gtaatggcgt ggctttccgc atgattgagg tggtagcgct
     1021 cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg aggaagcgca aaggcgtccg
     1081 gtggtgcatg ggcacttacg cgccggggct tagtggttct gcggtttcgc cggtggtctg
     1141 ggtagcatct ccagctcgtt aatcagcggt tgtagtcggt tcacatccgc ctgtcttgtg
     1201 acttcctttc gcagaaactg gagcaggaac gcacgcagtt gcgcttcttc cggcctccgt
     1261 accctcgcca gcatggcagc ccccacaatg actttttgcg ccgtgtccag gctccggctc
     1321 ttcgccttca ggcgctgtaa tctggcctca gcttcggcaa tcttctgttc gagtgttctg
     1381 ctcatttcgt gactccgtgc gcggtgaaaa atcgcatttt agcgcgtcac tggtagttta
     1441 aaaactaaac tggcataatg cacggcacat cacgaagtgc gcacttatac aatctccact
     1501 tcgtttcgat tgtgtgcggt ctgcgacgct aaaagaaaac ggcaaaaagg cattacggca
     1561 gaaatggcga tttatcatct cagcatgaaa atcatttcgc gaaaaaacgg ctacagtgct
     1621 gttgcttctg ctgcctaccg ttccggctct gtcatacccg atgaccgtac cggattaatc
     1681 cacgattaca cccgtaaacg cggcgttgat gatgcggtca ttctcacccc tgcgaatgca
     1741 ccgtcctggt gtggtgaccg ttccgttctc tggaatgcgg tcgagaaagc cgaacagcga
     1801 cggaactccc agctggcaag ggagattgaa ctcgccattc cccgtgagat ttcccgcgag
     1861 gccgcacggg agaccgttct cgcttttgtc cgggaaaact ttgtcagtcg gggcatgatt
     1921 gccgatgtgg cgttccatca catggaccgg accaatcccc atgcgcacat catgctgacc
     1981 acgagagctg tcggggaaac gggattcgca ggaaaggtca gggactggaa cgaccgggca
     2041 ctcgccgaga cgtggcgcgc atcatgggct gaccatgcga acagagcgct tgcgaacgcc
     2101 ggctaccagg aagagataga ccatcgttca tacgagcgtc agggactgga gaaagcgccg
     2161 ggccttcacc tcggaaaggc tgcctgtgcg atggaaaaac ggggaatgga aacagaacgc
     2221 ggtgagcaga accgtctgat taacagcctt aacctggaaa tacaggtttc ccgcacgcag
     2281 cttgctctca ggacggttca ggaagcgcag cgtaagcggg aactcagcga tgctgcacgt
     2341 cgtgcagcgg aagcccttaa cctgaccatt cccgctgcga atgcctcagc ggataccctg
     2401 cgggaattca ttgccacgct gccgcaggaa tgcgggaacg cgtgggagat gaccccggag
     2461 ttcctggcga tgagcgggaa ggtgaacgac atcgaacgtg aggggaatgc gctgctgaaa
     2521 gagcaggcca ttctcgaaaa ggagatgacc ggactcaaaa aagcacgccc tgtcgcgtcc
     2581 cttctgtcag agattcccct gatgacatgg gctgaaccgg aataccgcaa aagacaactc
     2641 cgtttctgga aactcgggaa acagattgaa tctcttcgcc gcacctacag ggccgtgaaa
     2701 gaacgggaca ttcccgcccg tcgtcaggcc tttgaaacgc agtggaatac gtggattgcg
     2761 ccgggaatgg cagagctgaa agaaaaactg tcagcacggg aagcggagcg gcgcagggag
     2821 gaagccgaag cggaagcgcg ccggaaggaa caggagcatg aggcgcggct gaaacgtcat
     2881 gataaccacc gtctgagccg tgaaacggca ttagtcgggg ttattacgga gctggggcgt
     2941 gccagagagc cgggaacggg caggataacc cgctacatga tgctgagtaa cagagccgga
     3001 gaattcacgg tatggggtga tgagctggcg cattaccccc agagtgttca tgacccggtg
     3061 aatgtttacc tgtcgccagg cggggctgtg atggtctcgg atatacgtga gggaatgcca
     3121 gaatctcatg agacgatggc gcggcctgag cgtgtgagaa tgtattccgg tgcgacggtc
     3181 cggcatgtac tggaacagat gcgccagggg tggccctctt acggttttcc ggcgctgccg
     3241 catcactggc cggataattt ttatttcagc gacgaccgca ggcccgtagc ctctccgctg
     3301 ccgtcagcgc agcgggtgga tatcaccgct gatgctgtac cggaacaact catgcctgtt
     3361 gtattttcga gagagggaaa cagcaggacg ctgaatctgt tgttatgcaa agggccggag
     3421 gaaatgcttg tcgggtttgt tcgccgggaa ggagggtttt gccccgttct tgtgcttccg
     3481 tcgccggatt acagtcatct gatagtcagc accatcacgg agaacggggt atgcctggca
     3541 ggttacggag aagctataaa ccatgatgag gatactccgt acccaccgga accgcacctg
     3601 atgcagttcc ggctcaaagg ccatcatgac aggcttttgg ctgctgtcca caaaccggaa
     3661 gagatgccgg attatctctt ccgtcaactc ggttttaatc agacctggca tgagtggaag
     3721 cgggacgaac agcacaggca acaacaacgc cgccccgggc acttccgggg catgagtatg
     3781 tgatgtccgg ggctgcaccc cggaccccgc caacacatca cgggccacaa aattttttgt
     3841 ggcccgctct gcgttttcta agtgttatcc ctcctgattt ctaaaaaatt ttccacctga
     3901 gcttgacaga aaaaacgatg acgggtactt tttgatctgt acataaaacc agtggtttta
     3961 tgtacagtat taatcatgta attaattgtt ttaacgctta aaagagggaa tttttatgag
     4021 cggtggcgat ggacgcggcc ataacacggg cgcgcatagc acaagtggta acattaatgg
     4081 tggcccgacc gggcttggtg taggtggtgg tgcttctgat ggctccggat ggagttcgga
     4141 aaataacccg tggggtggtg gttccggtag cggcattcac tggggtggtg gttccggtca
     4201 tggtaatggc ggggggaatg gtaattccgg tggtggttcg ggaacaggcg gtaatctgtc
     4261 agcagtagct gcgccagtgg catttggttt tccggcactt tccactccag gagctggcgg
     4321 tctggcggtc agtatttcag cgggagcatt atcggcagct attgctgata ttatggctgc
     4381 cctgaaagga ccgtttaaat ttggtctttg gggggtggct ttatatggtg tattgccatc
     4441 acaaatagcg aaagatgacc ccaatatgat gtcaaagatt gtgacgtcat tacccgcaga
     4501 tgatattact gaatcacctg tcagttcatt acctctcgat aaggcaacag taaacgtaaa
     4561 tgttcgtgtt gttgatgatg taaaagacga acgacagaat atttcggttg tttcaggtgt
     4621 tccgatgagt gttccggtgg ttgatgcaaa acctaccgaa cgtccaggtg tttttacggc
     4681 atcaattcca ggtgcacctg ttctgaatat ttcagttaat aacagtacgc cagcagtaca
     4741 gacattaagc ccaggtgtta caaataatac tgataaggat gttcgcccgg caggatttac
     4801 tcagggtggt aataccaggg atgcagttat tcgattcccg aaggacagcg gtcataatgc
     4861 cgtatatgtt tcagtgagtg atgttcttag ccctgaccag gtaaaacaac gtcaggatga
     4921 agaaaatcgc cgtcagcagg aatgggatgc tacgcatccg gttgaagcgg ctgagcgaaa
     4981 ttatgaacgc gcgcgtgcag agctgaatca ggcaaatgaa gatgttgcca gaaatcagga
     5041 gcgacaggct aaagctgttc aggtttataa ttcgcgtaaa agcgaacttg atgcagcgaa
     5101 taaaactctt gctgatgcaa tagctgaaat aaaacaattt gatcgatttg cccatgaccc
     5161 aatggctggc ggtcacagaa tgtggcaaat ggccgggctt aaagcccagc gggcgcagac
     5221 ggatgtaaat aataagcagg ctgcatttga tgctgctgca aaagagaagt cagatgctga
     5281 tgctgcatta agtgccgcgc aggagcgccg caaacagaag gaaaataaag aaaaggacgc
     5341 taaggataaa ttagataagg agagtaaacg gaataagcca gggaaggcga caggtaaagg
     5401 taaaccagtt ggtgataaat ggctggatga tgcaggtaaa gattcaggag cgccaattcc
     5461 agatcgcatt gctgataagt tgcgtgataa agaatttaaa aactttgacg atttccggaa
     5521 gaaattctgg gaagaagtgt caaaagatcc cgatcttagt aagcaattta aaggcagtaa
     5581 taagacgaac attcaaaagg gaaaagcacc ttttgcaagg aagaaagacc aagtaggtgg
     5641 tagggaacgc tttgaattac atcatgataa accaatcagt caggatggtg gtgtctatga
     5701 tatgaataat atcagagtga ccacacctaa gcgacatatt gatattcatc ggggtaagta
     5761 aaaatggaac tgaaacatag tattagtgat tataccgagg ctgaatttct ggagtttgta
     5821 aaaaaaatat gtagagctga aggtgctact gaagaggatg acaataaatt agtgagagag
     5881 tttgagcgat taactgagca cccagatggt tcagatctga tttattatcc tcgcgatgac
     5941 agggaagata gtcctgaagg gattgtcaag gaaattaaag aatggcgagc tgctaacggt
     6001 aagtcaggat ttaaacaggg ctgaaatatg aatgccggtt gtttatggat gaatggctgg
     6061 cattctttca caacaaggag tcgttatgaa aaaaataaca gggattattt tattgcttct
     6121 tgcagtcatt attctgtctg catgtcaggc aaactatatc cgggatgttc agggcgggac
     6181 cgtatctccg tcatcaacag ctgaagtgac cggattagca acgcagtaac ccgaaatcct
     6241 ctttgacaaa aacaaagcgt gtcaggctga ttctgatgcg cttttttttt gaaatgtcac
     6301 aaaaattcca tgtgggagat gggatctaaa atccccgtgc agaactttcc atccaggggg
     6361 agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcgcatcg
     6421 ctttcgtgca tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc
     6481 ctggtgtttc cttgtctttt tgcgtttttt gcgtcagaaa gcgattgagg gcgttttaag
     6541 gggggcgtac aacgggagtt atggtaaatg gatcgggttt tcgggaagga tcgacaggat
     6601 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc cccgtaaatc gtgctctcac
     6661 cgccaagatt gatcacgaaa ttacagggcg ccgggttccg cgtttcccga tgggaaagcg
     6721 cggttagtta aactgtgtac cgagagaaat cgtatcac
//
