LOCUS       NZ_AP027169             6715 bp    DNA     circular CON 06-JUN-2024
DEFINITION  Escherichia coli strain H19 plasmid pO26_H19_4, complete sequence.
ACCESSION   NZ_AP027169
VERSION     NZ_AP027169.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562883
            Sequence Read Archive: DRR424511
            Assembly: GCF_027925625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yano,B., Taniguchi,I., Gotoh,Y., Hayashi,T. and Nakamura,K.
  TITLE     Dynamics and temporal change of Shiga toxin (Stx)-transducing phages
            in the O26:H11 Stx-producing E. coli lineage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Yano,B., Taniguchi,I., Hayashi,T. and Nakamura,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) Contact:Keiji Nakamura Kyushu University,
            Faculty of Medical Sciences; Higashi-ku Maidashi 3-1-1, Fukuoka,
            Fukuoka 812-8582, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Coverage                   :: 177x
            Sequencing Technology             :: MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027925625.1-RS_2024_06_06
            Annotation Date                   :: 06/06/2024 03:15:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,927
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,494
            CDSs (with protein)               :: 5,494
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 119 of 310
            Pseudo Genes (incomplete)         :: 188 of 310
            Pseudo Genes (internal stop)      :: 77 of 310
            Pseudo Genes (multiple problems)  :: 65 of 310
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027169.1.
            Annotated by DFAST v.1.2.18 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H19"
                     /serovar="O26:H11"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pO26_H19_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="1967"
     gene            complement(6..197)
                     /locus_tag="QMG77_RS29495"
                     /old_locus_tag="H19_p4_01"
     CDS             complement(6..197)
                     /locus_tag="QMG77_RS29495"
                     /old_locus_tag="H19_p4_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            262..585
                     /locus_tag="QMG77_RS29500"
                     /old_locus_tag="H19_p4_02"
     CDS             262..585
                     /locus_tag="QMG77_RS29500"
                     /old_locus_tag="H19_p4_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955994.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            794..>1225
                     /locus_tag="QMG77_RS29505"
                     /pseudo=""
     CDS             794..>1225
                     /locus_tag="QMG77_RS29505"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1263..1781
                     /locus_tag="QMG77_RS29510"
     CDS             1263..1781
                     /locus_tag="QMG77_RS29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001447511.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            1788..2021
                     /locus_tag="QMG77_RS29515"
     CDS             1788..2021
                     /locus_tag="QMG77_RS29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2396..2602)
                     /locus_tag="QMG77_RS29520"
                     /old_locus_tag="H19_p4_04"
     CDS             complement(2396..2602)
                     /locus_tag="QMG77_RS29520"
                     /old_locus_tag="H19_p4_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(2595..2978)
                     /locus_tag="QMG77_RS29525"
                     /old_locus_tag="H19_p4_05"
     CDS             complement(2595..2978)
                     /locus_tag="QMG77_RS29525"
                     /old_locus_tag="H19_p4_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(3369..4430)
                     /locus_tag="QMG77_RS29530"
                     /old_locus_tag="H19_p4_06"
     CDS             complement(3369..4430)
                     /locus_tag="QMG77_RS29530"
                     /old_locus_tag="H19_p4_06"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_000034299.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            4518..5675
                     /locus_tag="QMG77_RS29535"
                     /old_locus_tag="H19_p4_07"
     CDS             4518..5675
                     /locus_tag="QMG77_RS29535"
                     /old_locus_tag="H19_p4_07"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(5903..6061)
                     /locus_tag="QMG77_RS29675"
     CDS             complement(5903..6061)
                     /locus_tag="QMG77_RS29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     Typing          6559..6643
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(5990..6539)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.298"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          230..579
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
     Typing          576..2119
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgtgtcaga ggtcttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
       61 tcggcgtggt cgtgaagaga ttcacaaata tcggcctgtt catctgcgtc cagttcgttg
      121 agtttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      181 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
      241 ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      301 ttactggaac gctgtgaggg taaacaactg gcggtatgga tgcggcgggt ctgcctgggt
      361 gagccggttg cccgttccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
      421 gccgccatcg ggaataacct gaatcagaca gcgaggaagg taaacagcgg gcagtggtct
      481 tccggtgacc gggttcaggt ggtggccgca ctgatggcca tcgagggaga gttgcgcagc
      541 ttacggcagg tcgtcaggga gcagggagcg cgggatgatt gttaaatttc atgccagggg
      601 aaaaggcggt ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg
      661 ggcaacggtg cttcagggta atccggaaga agtcagggaa ctcatcgatg ccacgccatt
      721 tgcgaagaaa tacacgtccg gtgttctgtc gttcgcggag aaggaactgc cgccgggagg
      781 acgtgaaaaa gtgatggaga gctttgagcg cgtactgatg cccggtctcg aaaaaaatca
      841 gtacagcatt ctgtgggtgg agcaccagga caaggggcgt cttgagctga attttgtcat
      901 tccgaacatg gagctacaga ccggaaaacg cctccagccg tactacgacc gagcagacag
      961 gccgagaatt gatgcctggc agacactggt gaatcaccat tacgggctgc atgaccccaa
     1021 cgccccggag aaccgccgga tactgactct ctctgataac ctgccggaaa cgaaacaggc
     1081 gcttgctgag agcgtcacac gcgggataga cgctctttat catgtagggg agataaaagg
     1141 ccgtcaggat gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg
     1201 aagcagcatc agcattgcag acccgaacgg cgggaagaat atcaggctga aaggagcgtt
     1261 ttatgagcaa tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg
     1321 aatctacaga gaaaatgctg aacgacgagt tcaggaagct cggcgagtct gtcagcgagg
     1381 ctgtgacctc aaacgagacg aaaatcagag acgctatagc cctgttcaca gcctcgacag
     1441 agaaatcgct ggaaaaacac agggaagggg tgaaagaggc gatgatgcag cacaggaagg
     1501 acgtgttaaa gctggcaggg aatacgggca tgatgttact ggggatagtc tttctcctgt
     1561 ttaccgcgag tggcgggacg ctctggtatc ttggcgggag gatacaggcg aacctggaag
     1621 aaatcaggaa gcaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt
     1681 ttgtgcagga cgggagaagg aaatttctgg tcattccgca agggatgtca gcagaggtga
     1741 cgcactatca ggggaaggac tggatacaac tgacggagtg atacggaatg acagagctgg
     1801 aaacacattt gctgaacgcc ttagagcagc tgcaacagga ctatatgcag cggctgaacg
     1861 aatgggagag cgccttcgtg gaattgcaga agatgttttc gcttacgcaa cgggacaacg
     1921 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcgga
     1981 cagaacgctt gagccagtta tacagcgaga actggagata agagaggaac ggctgataca
     2041 ggagcgcgaa catgcgttat ccctggaacg ggagcgtcag ccggaaatac aggaacgcat
     2101 actggatggt ccttcgttgt cgctgtgatg gcgaaactat aaaaaatggc aggttcagtg
     2161 gattttgacg gactaatgta gtctgcacca tctggttgca taggtattca tgcggttaaa
     2221 atttatcagg cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg
     2281 ctgccgtgat cgcaccgaac gcgttttagc ggtgtgcgta acggatgtta tggtaaatac
     2341 aatatgcagc cagtcttaga gacgaaaagt ccgcccagaa gactgacttt tcagatcaac
     2401 cttcagcatc taacttgtct atcgcttttt tattgcgccg ataggtagcc attagtttac
     2461 catcgcctga ttgtaagtca cgacctgctt gctcagcata ggtatcgttt acagtcttag
     2521 cgcctttttt ataggccttt ttaacatcat caaatccggc ttggatgact tctttcggct
     2581 tttcttgagg taatttagcc atgctgtgaa tcctctataa ggttgattat ttttccaaca
     2641 gataagacgg catcttctgt gtcgctgatt ttatgattta tccaaccgtc ctctatcttt
     2701 aatttgttca ctactcctaa tgtaaaaaca atcttttttt caggaaagaa aattgcaaag
     2761 gttctacttt tctctttcca atcagagggc ggcgtcgaac ggaacgctac attcctcgat
     2821 ttaagtatcg ctttgatttt actaattaat tctttttttt gtttattgct gagatttttg
     2881 acgaaccctt tgctgagatt gtctgtgtaa tcatcaaaag aaatatcaac tggtttgttt
     2941 ttttgttttt tatcagatag atgaacaatg tttgacactg gatatcctgc tttttgatga
     3001 atgaatactc agaagagtag cggaactgtc ttgttagtca cagaggtaga gtatcaattc
     3061 tacgatagat gggttcgaag atccggctga gaccgttcag tacgcggtcg agatgttatg
     3121 ttcaatgtaa tgaccgtcga aattgccttt acgggcatga agcaaccagg agcaatcatt
     3181 ggcaaaggta actagagttc aatgcatggt aatcattttt gagcttgtga tgcatagagt
     3241 tggttgctgt tccattgcgt ctcaaactcc gtcaagatgc tcgccgtaaa agcgagcatg
     3301 tctagtatgc ctgttccgct actgattttt gatgggcgaa ttcagggctg gcgagccacc
     3361 aagaaaaatt actttatttc atcaaacagg tgagatgcag tataataaac gctttgatca
     3421 aagcgtctct ctgggcttaa tttattttgc ccgccattta aaattatttc ttttaatact
     3481 gatggtggga ggatgggttt tgttcttctt gggtgatcca tataagcttt tgttattttt
     3541 ccgtttagcg agaactcttt atcactttga tatccaaagt tcatctcagt ataaattcta
     3601 ttgatgtttg tcttgttttt gctcgcaatt tcatacacca ttctggcgag tctggctgat
     3661 gatctcatta gtctgctggc agatgaattg ttatatgagg atctatcaat aaaggttcga
     3721 cacaaagcaa aatcactcca gatgaatatg tcaaaggcat tttgatctaa tattgggctc
     3781 ttgccttttg ttttccataa tggttgcatt aaaaaaggtt tctggtaatc aagaaaattt
     3841 tcttggaaag tgtttaggca atttaatata ttttctttct ttgaaagaac ttcagtttca
     3901 ttcccccagt gctgtatgct ttcgcatgtg gattcaaata tttttcttat ttcatggaat
     3961 ttatcgctgc aagagtgcat tatgccaagg gcacaataag aagtgcttgc aggtcttatt
     4021 acaatctctg aactccattc gctctcgttt tctttatatg tcgtgctatc tggaagtacg
     4081 gttaatttta tttctagtgc ttttaaaaaa ctattgtttc cttttataac tagatctatt
     4141 ccttttatgt catcataagc atactgttgg tatggttcat attttttctc aaattgaaat
     4201 tcaagatcac tattttttaa tgttgtgtta aaaacgaagt caaaagatac ttctttggca
     4261 tgcactttta aatccttatc taaataaaga taaatcggat tcattttgtt gtctctcata
     4321 taacatgcta gagaggtggg aaaagaactg ttaaattggt tctttcccca cagatcgctc
     4381 tctttcctgt tggtgttttt taaaccataa agagattcta ttttgctcac tttttgctcc
     4441 tgacgtcaat ttattgacgt ctatggtgtg tttctatatc atgatgtgga gtgatatgat
     4501 aatgagtttt ttatgttatg agaataattg atctttttag tgggtgtggg gggttgtccc
     4561 taggcttttt gaaggggggg tttgatgttg taggtgctta tgatttctgg gatcctgcta
     4621 ttgaatgtta cagagataat tttagccatc cgataaaaaa actagatttg agcaatgttg
     4681 atgatgtggt gagggagctt aaagatatag acttcgatat gattattgga gggcctcctt
     4741 gccaagactt cagtcatgca ggtttaagaa ttgaaggtgc tagagctaac ttaactcgct
     4801 cattttcaga aattataaaa agaataaagc caaagtggtt tgtgatggaa aatgtagatc
     4861 gggcattaag aagtggtgcc tatttagaag ctcgaggaat ctttaaagaa tcagggtatg
     4921 ggttaacaga aattgttttg gatgccagta agtgtggtgt tccgcaaaaa agaaaaagat
     4981 tatttgtaat tggaaagctt gacgtgaggg atggttttat ccttaacgaa gtgatgtgcg
     5041 ggatatcgaa agatagtatg acagttagaa attatcttgg tgattcattg gggattgagt
     5101 attattacag acatccgaga aattataaca gaagggctat tttctctatt gatgaacctg
     5161 cgccaacagt aagaggggtt aatagaccga tacctgatgg atatttaggg catgcaggcg
     5221 atcctgtcag tatttctgag aatgtaaggc cattaacaac ctttgaaaga gcaagattgc
     5281 aaacatttcc tgaagacttt aaatttaaag gtgcaaaaac aaatttggag caaatgatag
     5341 gtaatgctgt tccggttgag ttagcaaaat atgttgcggt gacaatcatg gagtatgaaa
     5401 aaaaacaagt aaaaggtatt tatgataaag aaggctttag ggcgtggttg ctgaatgaaa
     5461 agaaactaac aaaaagaacc tctagcgata ttatatctag atgttgcaga ggtgtttcat
     5521 tttttgattc agagggggtg gatttttata actgtgaaat agatgaaatt attatgaaac
     5581 tggaaaggct tgaatcgttt gtcaggcttg gggttagcct gaagtctcag ttaagaaggg
     5641 cttttaaatt atattacgaa tattgtcgaa ggtaataaat acgaatttat gtctctcagg
     5701 ggagggggag gggataaaaa gatcaaagga tcctcttgtg atcctttttt tctgcgcgta
     5761 atctgctgcc tgtaaacaaa aaaaccaccg ctaccaacgg tggtttgttt gccggaatct
     5821 agagctacca actctttctt ccgaaggtaa ctggcttggt ggagcgcaga taccaaatac
     5881 tgtccttcta gtgtagccgc agttaggcca ccacttcaag aactcttaat atctcaatcc
     5941 accttgtcca gttaccagtg gctgctgcca gtggcgttaa gtcgtgtctt tccgggttgg
     6001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6061 cacagcccag cttggagcga acgacctaca ccgaactgag ataccaacag cgtgagctat
     6121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6241 ctgtcgggtt tcgccacctc tgacttgagc gtcgatttct gtgatgctcg tcaggggggc
     6301 ggagcctatg gaaaaacgcc agcggcgcgg ccttttgttg tcctttgcct ttttgctcac
     6361 atgttctttc cggccttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6421 gccgactccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     6481 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     6541 tggtgctctt ttcttataat ccgcactgat gccacatagt taagccagta tacactccgc
     6601 tatcgctacg tgactggttc agggcttcgc cccgaaaccc cctgacgcgc cctgacgggc
     6661 ttgtctgctc ccggcatccg ctcacaggca agctgtgacc gtctccggga gctgc
//
