LOCUS       NZ_AP026955             5410 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Salmonella enterica strain SE20-72C-2 plasmid pSE20-C72-2-7,
            complete sequence.
ACCESSION   NZ_AP026955
VERSION     NZ_AP026955.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00515066
            Sequence Read Archive: DRR396386, DRR396387
            Assembly: GCF_025999715.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Nakayama,T., Yamaguchi,T., Phuc,N.D., Chinh,D.V., Jinnai,M.,
            Kumeda,Y. and Hase,A.
  TITLE     Complete genome sequence of Salmonella enterica SE20-C72-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Nakayama,T. and Yamaguchi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2022) Contact:Tatsuya Nakayama Hiroshima
            University, Graduate School of Integrated Sciences for Life; 1-4-4
            Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 187x
            Sequencing Technology             :: Illumina iseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:37:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,969
            Genes (coding)                    :: 4,785
            CDSs (with protein)               :: 4,785
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 66 of 184
            Pseudo Genes (incomplete)         :: 133 of 184
            Pseudo Genes (internal stop)      :: 28 of 184
            Pseudo Genes (multiple problems)  :: 40 of 184
            Pseudo Genes (short protein)      :: 1 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026955.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="SE20-72C-2"
                     /isolation_source="Gut content of Anabas testudineus"
                     /host="Anabas testudineus"
                     /db_xref="taxon:28901"
                     /plasmid="pSE20-C72-2-7"
                     /geo_loc_name="Viet Nam:Ho Chi Minh City"
                     /collection_date="2020-03-18"
     gene            complement(95..388)
                     /locus_tag="OO990_RS25320"
                     /old_locus_tag="SE2072C2_48670"
     CDS             complement(95..388)
                     /locus_tag="OO990_RS25320"
                     /old_locus_tag="SE2072C2_48670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609279.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLEVSQSALSRLLN
                     GKTDLSYEMALRLSKVIGRSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            726..1007
                     /locus_tag="OO990_RS25325"
                     /old_locus_tag="SE2072C2_48680"
     CDS             726..1007
                     /locus_tag="OO990_RS25325"
                     /old_locus_tag="SE2072C2_48680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000594459.1"
                     /translation="MIKNIKHKGLRSFAENGNTAGIQPHLAKKLRMILTTLSTATCLQD
                     IVDVRALGCHPLKGERKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2259..2582
                     /locus_tag="OO990_RS25330"
                     /old_locus_tag="SE2072C2_48700"
     CDS             2259..2582
                     /locus_tag="OO990_RS25330"
                     /old_locus_tag="SE2072C2_48700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_171853543.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2791..>3225
                     /locus_tag="OO990_RS25335"
                     /pseudo=""
     CDS             2791..>3225
                     /locus_tag="OO990_RS25335"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3260..3751
                     /locus_tag="OO990_RS25340"
                     /old_locus_tag="SE2072C2_48710"
     CDS             3260..3751
                     /locus_tag="OO990_RS25340"
                     /old_locus_tag="SE2072C2_48710"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045342089.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGIQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3758..3973
                     /locus_tag="OO990_RS25345"
                     /old_locus_tag="SE2072C2_48720"
     CDS             3758..3973
                     /locus_tag="OO990_RS25345"
                     /old_locus_tag="SE2072C2_48720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1973..2146
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(1462..2049)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.54"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2573..4065
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcatttcaa gatctaagaa ctgaactaaa aatcaaacta aaggtaggta tgcgggggtt
       61 ttacggcaaa aagcccgcac gagcgggctt ttacttatgg aaacatcgga tgaaggtgtt
      121 ctagcgtctc ggataaagtt ttacgacttt tcgagaggct gtactgagcc tgcatattaa
      181 cccacgactc tgcagagcgg cctataactt tagatagtcg aagagccatc tcataactaa
      241 gatcagtttt tccattgagc aatctactta atgcagattg tgatacttca agcttatcag
      301 ctaactctgc ggcaggtatt tcgaacggtt ccatgaacat ttccctgatg aattcaccgg
      361 ggtgtacagg atcgtaaaga tttatcatgc cttcctctat tggttttgta tttgattaca
      421 caaaacattt tgtttctttc gcgttgcgca ttgcacaata cgcaagtgtc tgttattgac
      481 aaaaaaagca gtaagcttta ggatgctcgg tggagcagaa agctaccgct gatatggtgt
      541 ctttaccgac tttttcaagg cgccatattg tacggttagc gctcttggaa gtcaaacgag
      601 gtaatcgacc tcagagacaa agttacgtca attattttaa taatgcaagg ggttcaaatg
      661 gacgctgtca aattgttttc tgtttataga gatagatatc gacgaaaatt ttatggagat
      721 gattgatgat aaaaaacata aagcataaag ggcttcggag ttttgctgaa aacggtaaca
      781 ccgccggaat tcagcctcac ttagcgaaaa aacttcgcat gatattgact acgctaagca
      841 ccgcaacatg tcttcaagat attgtggatg taagggcgct ggggtgtcac cccctaaaag
      901 gtgaacgtaa aggtgagttt tccgtagagg ttaacggaaa ctggagggtg gttttcaagg
      961 tggaagaacc aaacgtctta gacgttgatc ttgaagatta ccattagtaa tcaataagta
     1021 agttactaat ccatcctagt ggcaatatgg atggattagg gactttattc aaaaataagt
     1081 acctctggag gatttatgag agcctttatg attttgttgt tccctttgct ttgtgtagtc
     1141 attgctgggg ttcttgctgt tcgtggccta gatgggtggg gatggttcct gtttgcagct
     1201 gttttgtgta ctggtgttga tacggtgcag aaacatgtct ctaacaagaa taattctgta
     1261 agcgattgag gctttcaggg ttcttccttg actgattaaa tcgacaaagc gtatcttatg
     1321 ttcgaaacga aaaaaccgcc tggggaggcg gttttttcga gggttcagta agttggggaa
     1381 cttctgaacc gtggtaacag ggtgtacaag accgctgaaa ccaaattctg tccttccagt
     1441 ttagcctcag tatggctctg acttcaagaa ctctgcatca gtaatctctt gtacaccctc
     1501 ttaccaatgg ccgcagccag tggcgttttg ttgtgtcttt ccgggttgga ctcaagtcga
     1561 tagttaccgg ataagacgct gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     1621 ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca
     1681 taacagcgga gtgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     1741 accaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     1801 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgtcgt
     1861 ggccttctcg cttccctgtt aagcttcttc ctggcatcct ccaggaaatt tccgccccgt
     1921 tcgtaagcca tttccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1981 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgccgc cttttttctc
     2041 ctgccacatg aagcacttca ctgacaccct catccgtgcc aacatagtaa gccagtatac
     2101 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgcc aaaagcctga
     2161 cgcgcctgcg gcttgtccaa ccccgcgccg accgggaaag gctttcccgc ccgtcccggc
     2221 gttatcagga ggccggtttg tcagagaaac ggagcaagat gctcaccatg tgggtgacgg
     2281 aggacgagca ccggcggctg ctcgagcgct gtgacggcag gcagctggcg gcgtggatgc
     2341 ggcagatctg cctggacgaa aagccggcac gttccggaaa gctcccctca ctctcgccgg
     2401 cgctgctgcg ccagcttgcc ggcatgggca ataacctgaa ccagatagcc cgccgtgtta
     2461 acgccggcgg cggcaccggc catgacaggg tacagattgt ggcggcgctg atgaccatcg
     2521 atgccggcct tgagcggctg cggcacgccg ttcttgagaa aggaacggac gatgatcgtt
     2581 aaatttcatc cgcgcggccg cggcggtggc gccgggccgg tggactatct gctgggtaaa
     2641 gaccggcagc gtgaaggcgc caccgtcctg caggggaagc cggaagaagt ccgggagctg
     2701 attgacgcgt cgccttacgt taagaaatac acctccggcg ttctgtcgtt tgcggaggcc
     2761 gatctgcctc ccggccagcg ggaaaaactg atggccagct tcgagcgggt tctgatgccc
     2821 ggactcgata aagatcagta cagcattctg tgggtggagc acgcggacaa ggggcgtctg
     2881 gagctgaact ttctgatccc caataccgag ctgctgaccg gcaggcggct ccagccttac
     2941 tacgaccgcg ccgaccggcc gcgcatcgat gcctggcaga ctatcgtgaa cggcagactg
     3001 gggctgcacg acccgaacgc gccggaaaac cgccgggcgc tggtcacgcc gtccgggctg
     3061 ccgaaaacga agcaggaggc cgcagaagcc attacccggg gactgctgac ccttgcttcg
     3121 tccggggagc tgaaaagccg tcaggatgtc actgacactc ttgaaggtgc aggttttgag
     3181 gtggtgcgca ccacgaaaag cagtatcagc attgccgacc cggacggggg gcgaaacatc
     3241 cgactgaagg gagccatcta tgagcagtct tttaacgctg gcgaaggact tagagcagca
     3301 atcgaaagcg cagcagcaga gtaccggcga gatgctgaaa gccgcattca gcgagcacga
     3361 gcagtctgtc agagcggaac tgagcgcaag cgcgaggaga atcagcgacg ccatcctcgc
     3421 ccacgagcag agtatgagcg aggcgatgga gaaaaaccgg cggagcgtac tgcggactgc
     3481 cgggcggaca tggctcacca tcctgatggt gtcagcgctg ctgatcggca cgagcgggag
     3541 cattctgtgg tggcaggggc agcagataac cgacaattac acgcatcttc gtcagcagga
     3601 agataccctg gcgaaaatga ccgcccggac gtggggagtg cgctatcagg agagcagcga
     3661 cggccggagg tttcttatcc tgccgccggg gatacaaacc gaagccatcc cttacgacgg
     3721 gacgacatgg atccgcctga aacaggagta gctgaaaatg acagagctgg aaaagcagtt
     3781 gctgagcgca ttagagcagc tacagcagga ctactcgcaa aggctggacg aatgggagag
     3841 cgccttcgcg gaatggcgga gcatgtgtgg gcttatgcaa cgggagaacg cggtgctgaa
     3901 cgagcgcgtg tcggacttga gcacgcaggt gctgagttta agcgagcagc tgcgccgctt
     3961 gtccgggaac tgaatgccat cgagctccgg cagagggaga atgagtatca gaaaacactg
     4021 gagctggagc gccagccgca gaaaacctac cgcgggccga cactttaaac catccaggca
     4081 gctgacaacc cgcacgaaga ggggctctta tttctcccgc taaataggtt cgtgatcaaa
     4141 aaggtcacga cttattcagg agcgggaaat gaagctgtta aatcttattg aatcattgct
     4201 taactgcctg tatctggcgg cggagctgtg ggactggctg gagaagcatt tgtcggtttt
     4261 taaggcattg gcgcggattt tagtgtgact ttaacatcca tgcatctggc tgcatgacta
     4321 tgcactagtg taatatccag cggctgcgtt tctggtgttt tttcgtcatg tctgtcgcca
     4381 ctttcctgag tctggcaccg gaaacgcagc caaccccgtc tcagcggtgc gggcaacgga
     4441 tgttatggta aataagagcg accgtcccgg tcgctcttga aagagaattc ggccttaggt
     4501 cagttttcgc agatacaatc atgtggactg cggagacatt cttcgcactc ctcacatatg
     4561 cactcatcca gtttttcacc gcactcgtcg caacgtgagc agctacagtg ctcatctaaa
     4621 tttccacaat cgctgcagcg ctcacagacg cagtctgcca gcttctcgtt gcagtcatcg
     4681 cagaagccac aggtacaggc gctggcaggg agggcacaaa catcgcaaat cttatccatt
     4741 ggatccattt tatctcactt ttattttatc agtggctgtg tgcgggcgat ttccccagcg
     4801 cgatagatgt ggatttaagt gccatttttc aatgattaag gcagctaaaa aagtggtttt
     4861 ttagggcttt tcaacgtgac cagcactgac ggggataaat acaggtaagc aataatgctc
     4921 agtcatgaaa aggagaatcc gatggagcaa catacccctg aatacttacg acgtacctta
     4981 gctcataacc gtgccctaat ggacgatatc attagcagtg gcatgtccag gtactacaac
     5041 acggagattg tggatgccgc atgtgaggcg atagaagctg aattgcgacg acgaggaatt
     5101 ttgtaagaga caaagcccgc ttatgcgggc tttgtcgtta ctggcgcttc tgttactcag
     5161 tgttgtctgg ggttcggtag atctgtagcc agtacctgcc aggtgcgctc gttggtggtg
     5221 cgaactcatt atcgcgaatg gtgcagtatc gtccatattt gcgggtaaca gtatgagtgt
     5281 ttgtgttgat gtcacattct tggaatgagc caccatcacg acctattttg ccatgtggtc
     5341 ctacataaat tgccattttg ttctcctttt tagagccttg tatgggcatc ttattggtgt
     5401 tcaaacaaca
//
