LOCUS       NZ_AP026952             9869 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Salmonella enterica strain SE20-72C-2 plasmid pSE20-C72-2-4,
            complete sequence.
ACCESSION   NZ_AP026952
VERSION     NZ_AP026952.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00515066
            Sequence Read Archive: DRR396386, DRR396387
            Assembly: GCF_025999715.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1
  AUTHORS   Nakayama,T., Yamaguchi,T., Phuc,N.D., Chinh,D.V., Jinnai,M.,
            Kumeda,Y. and Hase,A.
  TITLE     Complete genome sequence of Salmonella enterica SE20-C72-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Nakayama,T. and Yamaguchi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2022) Contact:Tatsuya Nakayama Hiroshima
            University, Graduate School of Integrated Sciences for Life; 1-4-4
            Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 187x
            Sequencing Technology             :: Illumina iseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:37:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,087
            CDSs (total)                      :: 4,969
            Genes (coding)                    :: 4,785
            CDSs (with protein)               :: 4,785
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 66 of 184
            Pseudo Genes (incomplete)         :: 133 of 184
            Pseudo Genes (internal stop)      :: 28 of 184
            Pseudo Genes (multiple problems)  :: 40 of 184
            Pseudo Genes (short protein)      :: 1 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026952.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="SE20-72C-2"
                     /isolation_source="Gut content of Anabas testudineus"
                     /host="Anabas testudineus"
                     /db_xref="taxon:28901"
                     /plasmid="pSE20-C72-2-4"
                     /geo_loc_name="Viet Nam:Ho Chi Minh City"
                     /collection_date="2020-03-18"
     gene            39..2168
                     /locus_tag="OO990_RS25200"
                     /old_locus_tag="SE2072C2_48450"
     CDS             39..2168
                     /locus_tag="OO990_RS25200"
                     /old_locus_tag="SE2072C2_48450"
                     /inference="COORDINATES: protein motif:HMM:NF037202.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_319963372.1"
                     /translation="MHILDPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLA
                     YYIAAINIEAVYHSIMEESEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKAL
                     DIRVIVGNPPYSAGQGSANDNNANISYPGLDKRIKDTYALNSKATSTKNLMDSYIRAIR
                     WASDRVGEQGVIGFITNAGYIDSNSTDGLRKCLIDEFSSIYVFHLQGNSRRSSGELAKK
                     EGAGIFEIRTPVAITILVKNRERKSKGELKFFKIGDYLSREEKLEFISKVSSVDGAEIK
                     SKWESIVPDSYNDWLNQRDDEFNKFIKIGDKKERENTIFESYSLGIATGRDAWVYNFSL
                     EKLKFNLTGFIDFYNSELKRYSEYNDKNMMVKNFVTIDTSKISWNDNLFSELKRGTSIN
                     FSESNIFHVSYRPFSKQHGYVSKELIARRYQMAQIFPQPNSHNLIISVTGLGGKKGFST
                     LMSNCIVDLNSMDAGAQCFPLYLYEESSGSDLLDGKVNGDYISKHAIVDNGLKYFKDFY
                     QTNAISKEDIFYYIYGLLHSDDYRARFCDNLTKELPRIPHVKKISDFWLFSKAGRELAE
                     LHVNYETVEPYKADIDTGSLSYSQLGKDDFYVEKMKFAKKGEKDTVIYNNKIRIKNIPT
                     EAYDYVVNGKPALEWVMERQGVSTHKDSGIVNDANDWAVETMNNPRYPLELFLRIITVS
                     LETQKIVNSLPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            2185..2427
                     /locus_tag="OO990_RS25205"
     CDS             2185..2427
                     /locus_tag="OO990_RS25205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139947263.1"
                     /translation="MEKPSCTILMIFMNPVVFNIMDLARTTATPLTGKTRRNVQNTGLS
                     APKTGCFGVAGLLSEWRGWFQPLCRLKPLFLPELP"
                     /name="hypothetical protein"
     gene            complement(2404..2853)
                     /locus_tag="OO990_RS25210"
                     /old_locus_tag="SE2072C2_48460"
     CDS             complement(2404..2853)
                     /locus_tag="OO990_RS25210"
                     /old_locus_tag="SE2072C2_48460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_139947264.1"
                     /translation="MALVSISEAARLTGKSRTTVQAYIKQGKLTKCTDSAGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGITVQRLAGEFVQLTDQNLLHENYALKAEVELVKALLREKE
                     LLLNEKDKRNEDLKQALLLIESKLPATPEPTMPPVVKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2861..3817)
                     /locus_tag="OO990_RS25215"
                     /old_locus_tag="SE2072C2_48470"
     CDS             complement(2861..3817)
                     /locus_tag="OO990_RS25215"
                     /old_locus_tag="SE2072C2_48470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094396512.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYASFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDDFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKDPISKKEDKSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            complement(4413..4880)
                     /gene="traD"
                     /locus_tag="OO990_RS25220"
                     /old_locus_tag="SE2072C2_48490"
     CDS             complement(4413..4880)
                     /gene="traD"
                     /locus_tag="OO990_RS25220"
                     /old_locus_tag="SE2072C2_48490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_094396501.1"
                     /translation="MNKSEKQEQWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            5043..6158
                     /gene="mobA"
                     /locus_tag="OO990_RS25225"
                     /old_locus_tag="SE2072C2_48500"
     CDS             5043..6158
                     /gene="mobA"
                     /locus_tag="OO990_RS25225"
                     /old_locus_tag="SE2072C2_48500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            6171..6755
                     /locus_tag="OO990_RS25230"
                     /old_locus_tag="SE2072C2_48510"
     CDS             6171..6755
                     /locus_tag="OO990_RS25230"
                     /old_locus_tag="SE2072C2_48510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            6868..>9612
                     /locus_tag="OO990_RS25235"
                     /old_locus_tag="SE2072C2_48520"
                     /pseudo=""
     CDS             6868..>9612
                     /locus_tag="OO990_RS25235"
                     /old_locus_tag="SE2072C2_48520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /name="DEAD/DEAH box helicase family protein"
     Typing          complement(2862..3817)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.478"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5044..6155
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaaaaagga gttcggacaa acactcgccg acaaaggtgt gcatattctt gacccattca
       61 ccggaaccgg tacgtttatc acccgcctgc tgcaaagtgg tcttatcccg tcagataaac
      121 tggcttacaa gtttaaaaat gaaatccatg ccaacgaaat cgtcttgtta gcctattaca
      181 ttgccgccat taatattgag gcggtgtatc acagcatcat ggaagaatca gagtacacgc
      241 catttgaagg gatttgtctg actgacacct ttgaaatgta tgagaaagat gacctggtca
      301 gtgaggtttt ggttgataac agtgaacgcc gtaagcgtca aaaagcgctg gatattcgag
      361 tcattgtcgg gaatcctcca tactcagctg gtcagggctc tgcgaatgac aacaacgcaa
      421 atatatctta ccctggctta gataaaagaa ttaaagatac atatgcttta aactccaagg
      481 caacgtcaac aaaaaatctg atggattctt atattcgagc tattcgatgg gcaagtgacc
      541 gcgttggtga gcaaggcgta attggattta taaccaatgc tggctatatt gactcaaatt
      601 caactgatgg gctcaggaag tgcttaattg atgagtttag ttcaatatat gtatttcact
      661 tgcaaggtaa ttcaagaagg agttctggcg agttagcgaa aaaagagggg gctgggattt
      721 ttgaaataag aacaccagtt gctatcacta ttttagtgaa aaacagagag aggaaaagca
      781 aaggggaact gaagttcttt aaaataggag actatttgtc gcgagaggag aaattagaat
      841 ttatctcaaa agtttcaagt gttgatggtg ctgaaattaa aagtaagtgg gaatcgattg
      901 ttcctgactc atataacgat tggttgaatc agcgagatga tgaatttaat aaattcatca
      961 agattggaga taaaaaagaa agagaaaata ctatttttga atcttattca ttagggattg
     1021 caaccggtcg agatgcttgg gtttataatt tctcattgga aaaactgaaa tttaatttga
     1081 ctggatttat tgatttttac aattcagaat taaagcgtta ttctgaatat aacgataaga
     1141 atatgatggt taagaatttc gttactattg atacatcgaa gataagttgg aatgataatt
     1201 tattttccga gttaaaaaga ggaactagta tcaattttag cgagtcaaat atttttcatg
     1261 tttcataccg tccattttca aaacaacatg gttatgtatc gaaagagttg attgcaagac
     1321 ggtatcaaat ggcacagata tttccacagc caaattccca taacttaatt atttctgtaa
     1381 caggtttggg agggaaaaaa ggtttctcaa cattaatgtc aaattgcatt gtggatttaa
     1441 attcaatgga tgctggcgct caatgtttcc cactctattt atatgaggaa agcagtggct
     1501 cagatttact tgacggtaag gttaatggtg attacattag taaacatgca attgttgata
     1561 atgggttaaa gtattttaag gatttttatc agactaatgc cataagtaaa gaagatattt
     1621 tttattatat ctatgggtta ttacatagtg atgattaccg ggcgagattt tgtgacaatt
     1681 taactaaaga gttacctcgt attccacatg taaaaaaaat atctgatttt tggttatttt
     1741 ctaaggcagg tcgtgaactt gctgagttgc atgtaaacta cgaaacagtt gagccctaca
     1801 aagctgatat tgatacaggc tccctgagct attcccagtt aggcaaggac gatttttatg
     1861 ttgagaagat gaagttcgcc aaaaaaggtg aaaaagatac agtcatctat aacaataaaa
     1921 ttcgcataaa aaacattcca actgaagcct atgactacgt tgtgaatggc aagccagctc
     1981 ttgaatgggt gatggagcgg caaggtgtct ctactcataa agatagcggt attgtgaatg
     2041 atgctaatga ctgggcagtt gaaacaatga ataaccctag atacccacta gagctattcc
     2101 tgagaattat cactgtcagc ctggaaactc agaaaatcgt caatagcctg cctaaattag
     2161 atatctaatc gataggtgtg caggatggaa aaaccatcct gcacaattct gatgattttc
     2221 atgaatcctg tcgtttttaa cataatggat cttgcgcgca ccaccgccac accactcacc
     2281 ggcaaaaccc gccgtaacgt ccaaaacacg gggttatccg ccccgaaaac aggctgtttt
     2341 ggtgttgccg gtttgttatc ggaatggcgg ggctggtttc agcccctttg ccggctgaaa
     2401 ccgctatttc ttccagaatt gccatgattt tttcaccaca ggcggcatgg ttggttctgg
     2461 tgttgctggc agtttggact ctatcagtaa caacgcttgc ttgagatcct cgttgcgctt
     2521 gtctttttca ttgagtagta gctctttttc acgcagcaat gcctttacga gctctacttc
     2581 agcttttaaa gcatagtttt catgtagcaa attctggtca gttaactgca caaattcacc
     2641 tgccaaacgc tgcacagtga tacctgattg ttcactgcca acttgctgac cagtgaatga
     2701 accgaaaaca cgtagaagct ctgatgtgtc tagttttgaa gcgcctgcgc tatctgtaca
     2761 cttggtcagt tttccctgct taatgtatgc ctgtactgtt gtgcggcttt ttcccgttag
     2821 tcttgctgct tcacttattg atactaaagc catagatacc tcagattcgt ccgtacttgg
     2881 ctaaaatttt ctctagcgtg gttcgttgtt tttgagtcag ccatgtaaat gagccgttga
     2941 gatcatactt actctgcatg tcactcagga atttagcttc aaatccagta agttgtattt
     3001 tcgcagttaa agcatcatgc aatgttgttt ttagcccctc atgcagacga ttttcaggcg
     3061 ttaaacggat tgacttatct tctttttttg atattggatc ttttgctagc tcagtcacaa
     3121 ggtcaatttg tttatccagt tcaacttgaa agatcagcgt atcagcagga cgaccgcgct
     3181 tatcaataga tagttttata ttgctatatg tatttaaatc ttttgagatt ggttttaaaa
     3241 cccaatggtt tagtaattta tattccgggt agcttttttc taacattagc atgaatttaa
     3301 aatcatcaat acttattgaa atattggctt tatgtgtttt tctttgtgtt agttctttta
     3361 gcagccattc ataaatgcgc attgagtatt tgttttcaaa agacttaacg tgctcgaggt
     3421 tgtatttaat gaatctcttt aggtgaaata gcaatgggat tatgtattta ctaaatacta
     3481 aagttacttc ttgtgtttcc ctattgaact ctgcatattc agcccaatta aaaacacgca
     3541 tagatacagc acctgttgga tatattaact caatagaccg cttaaataat tctcgtgttg
     3601 atgtatttaa tcgtccccac gcattctcat aacttatccc cataaatgaa gcatattctg
     3661 agcatgaaaa tttaaccgtt cgttcagttt catctggtgt ttctaatgtg gggtttagct
     3721 ttgcaacaca aaacaggatt agtttcgttt cgtgttctgt taggtcataa cgactaaccg
     3781 caagctcatt tgctttgaat actaccaatt cagccatgac taaaaccatt ggttgtgatt
     3841 ttaaaataac ataacccttg gtattggtta tgtcaattac actggcataa ccttttgtgt
     3901 ggttgtgtgg attacctaat tttcacttaa ccttttggcg gaagatgcta atttttgctt
     3961 aacctttcct aatttttgct taacctttca tgtttttttt tgttttataa caacagctta
     4021 aaatctatag aataaagaaa gaatagaaaa gataaaaaag aatagatcat ggccttgtgg
     4081 ataactgcac gcctgagtca gttgatcgat atgataaaag gatgtcgcaa acgctgtttg
     4141 ctcctcttca aaacagacct taaaccccca aaatactgag atagcaccct cgcaagctcg
     4201 ggcaacccgc tgaacatttc ttttgtctcc gaccatccgg ctcctgaatc gctgttcatt
     4261 ctgtgaaaat cagttcgctt cgctcacagc tctggcaggg aatggggcta gatgtcacta
     4321 cagccgcttt tcatgggttc atacgaggaa atccactcat gaaacaagaa aagcccgcta
     4381 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     4441 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     4501 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
     4561 ttactgtcaa ccagacccgc caggataagc aatccggcag actggtacag agcatggtca
     4621 cgggctttac gggcggctct ggcttcggct cgcttttctg cctgtatcag gttcatgact
     4681 ttcgctctgg cttcctgcgc tttctctgct gcctgttcag ccttcatcag cagggacagc
     4741 gttttgatat cctgtgctgt tttatctgct ttatccgtca gtatcagcat aagtttctgt
     4801 tgctcattcg gcgactttaa gccgcgaatg tactgcactc tgtctgctgc ccattgttcc
     4861 tgcttttcgc ttttattcat gcccgtttat cctctgaatg ttaacgggat aattatgctc
     4921 aatggtggca gctaaggtca aattccacta caatccctga taacttttta ttatcagggc
     4981 gcacttagac gtttcttcac ttcgttcaga aacggtgcgc gccttcagcg ggataaaaca
     5041 cgatggcatc ctatcatctc agcgtaaaaa ccggcggaaa aggcagtgcc tctcctcatg
     5101 ccgattatat tgcgcgtgag ggaaagtatg cccgtgaaaa agactccgac cttgagcaca
     5161 aggagtccgg caatatgccg gcgtgggcag cgcataaacc gtccgagttc tggaaagccg
     5221 ctgacacgtc tgaacgtgcc aacggctgta cctaccgtga aatcgagatt gccctgccgc
     5281 gtgagttaaa acccgaacaa cgtcttgagc ttgtccgcga ttttgtccaa caggaaattg
     5341 gcgaccgcca tgcctatcag ttcgctatcc ataatccgaa agcagcgata gccggtggcg
     5401 agcagcccca cgcccatatc atgttctctg aacgcattaa tgacggtatt caccgtgacc
     5461 ctgagcagta ttttaaacgg gctaacacca aagagccgga gcgcggtggc gcaaaaaagg
     5521 cacgtttcgg ggaaacaccg accgaacgca aagaatacct tgttgcccag agggaacgct
     5581 gggctgacct ccagaataag caccttgaac ggtatcagca cgctgaccgg gttgattccc
     5641 gttccctgaa ggcgcagggc attgaccgtg agcctgagcg gcatctgggc gcgggtcagg
     5701 ttcagcgatt cgatactgag cagctacagg ccattcttga gcgcagggag gctgaaaggc
     5761 aggtgcagca gtcccgtgat gaacgtgaca gcgtaattga cgtgactacc tcgttacggg
     5821 aagccctcag cgagagggat actctgacgc taaaacagga gctgaagtca gagcctgagc
     5881 aggaatccca ttccggcaga acgtttgatt ttgaaaaaga gcctgacaag ctgaacgcac
     5941 tggtcagtga tgccatgaag gatatacagg aagaaatcga tcttcagtcc cttgttaacg
     6001 atgcgatggc ggagttccag gggatacatc aggagatgga aaggcagaga gagcgcgaac
     6061 gtctggcgga aaaacagcgt cagcaggaga aagaacgaca gcgactcgcc gaacagatac
     6121 gacagaagcc ggataaaggc tggtcattct cacgataggc aggattttgt atgagcgacg
     6181 aaattaaaca aattgccgcc cttatcgggc atcatcaggc gctggaaaag cgtgttactt
     6241 ctctgacaga acagtttcag gccgcctcat cacaattaca gcagcaaagt gaaacgctga
     6301 gcagggtcat tcgtgagctg gacagcgcgt ccggtaatat gactgacacc gtcaggaaat
     6361 cggtgagttc cgcgctcact caggttgaga aagaactgaa acaagccgga ttagcacagc
     6421 agaaaccggc gacagaagcg ctgaatcagg ctgccgatac ggcgaaagcg atgatccatg
     6481 aaatgcgtcg tgagatgtca cgctatacgt ggaaatccgc gatttatctt gtactgacga
     6541 tattcttcgt cctggcttcc tgcgtgacgg catttacgtg gtttatgaat gacgggtata
     6601 gccagatcgc tgaaatgcag aggatggaag cggtctggca aaagaaagcc ccgctggctg
     6661 acatatcgcg ctgtgacgga aagccctgcg ttaaggtgga cactcgcact acgtatggtg
     6721 ataaagagaa tacctggatg attattaaga aataacatca tagagcacag aacgctgtgc
     6781 gtcgatctcc ggcggagaaa gcattaatta aacgtgtcgg ggggatctga taaccccagc
     6841 aaataaagga cattaaggat atcaacgatg aatgcacttt ctgcattgct gacaaaaatt
     6901 gaacaggctt caccaacaca gcgggacaaa ggtacaacct ttgaaaacct ttgtgtgcag
     6961 tatttcctcc atgaacctaa atatgccgag ctgtacagtg acgtgctgag ctatggcgga
     7021 tgggtttctc agtacggtga aaccgttggg atcacgaaga aaaaagatga tggcattgat
     7081 ttggtcgccg taaccaaaac gggggagttt catgctatcc agtgcaaaaa ctacaatcag
     7141 actaaaatcg ccaaaaagga tattgatagt tttctggctg cctccgataa aacgtatttt
     7201 acgctccgct atatcgttgc ctctacggat aactggacgg aggaagctaa aaatatgctg
     7261 cgggacaagg ccgttcctgt cactgcgtta agcctgacag accttgagca aagcgcactg
     7321 gactggtcac aattcgattt tgatccggcc tacaagccgg tgatgaaagc caaaaagcaa
     7381 ctccggcctc atcagactcc cgcgcttgaa gccgttaaaa gagggctggc gactgccgat
     7441 cggggcaagc tcattatggc gtgcggcaca ggaaaaacct tcacctctct gcgtattgct
     7501 gaagccgttg ccggaagagg taaaaccgta ctgttccttg tgccaagtct ggcactgttg
     7561 agtcagacgc tggatgaatg gacgcaggat acgctgatag acctgagatg ttttgcggtc
     7621 tgctctgaca gtgatgtggg taagaaaaac catgatgata atgttgttgt cggtatcagc
     7681 gacctgaaat atccggcgac cacgaatgct agcagcctcg tcaaagcgtt taatcagccg
     7741 gatattttcg gcagtgataa gccgccttat atgaacgtgg tgttttctac ctaccactct
     7801 gttgaggtca tccatcaggc gcaaaaactg ggttttcctg catttgattt catcatttgt
     7861 gatgaagctc accggaccac aggtgcgacc tttgaagggg atgatgagtc cgcctttgtt
     7921 cgtattcacg acaacgccta tatcgccggt caaaaacgcc tgtacatgac cgcgactccg
     7981 cgcatttttg gagatgatgc gaaagagact gagggagtta ccctatgctc aatggatgac
     8041 aaaagtcttt atggggatga tttgtatgtc attaccttct ctaaagccgt ccagttgggc
     8101 attttgtgtg attacaaagt tattgtgctt gccgtggaag aaaagcatgt cagccgccgc
     8161 attcagtctc tgctgaagga tgaaaataat cagctcaaag tcgatgatgc ggcaaagatt
     8221 gtgggttgct ggaaagcact ggcaaagcag ggactggcgg gggcagatgg cgttcaccct
     8281 gatgcgatga aacgggctgt tgcgttctgt caggtgatag aaaaagactt tcgcggaaag
     8341 acgcataagg tcagttctaa actgatcacg gaaatgttcg gagcagtggt tgaggcttat
     8401 caggcatccg agatcgaact tctcagggaa accgcaccgg ataaggcgat tgacccatca
     8461 ttaaaacttc agtgtcaggt gaagcacgtt gacggcagca tgaatgccac tgagaaaaaa
     8521 tccaaaattg aatggctgaa agcggacacg gatgaaaatg tctgccggat attgtctaac
     8581 gtccgttgtt tatctgaagg cgtggatgtg ccttctctgg atgcggtgtt gtttttaacg
     8641 ccgagaagct ctcaggtgga tgtggtgcag tctgtcgggc gtgttatgcg gcgtgccgaa
     8701 ggtaaagagc tgggctatgt cattctgccg gttgtcattc ccgccggaga agagccggaa
     8761 aacgccttaa acaacaatca ggcctaccgt gttgtctggc aggtgctgaa tgcactcagg
     8821 gcgcatgatg accgctttga tgccatgatc aacaagctgg agtttaacgg taaagatacc
     8881 agcagaatgg aagtgatcgc ggtagccgat aaagtggtga agaaaactaa acggcagacg
     8941 aaaaaatctg aactggcagg aaaagcgcgt aaaagcagtg gcattggttc agccgtgagt
     9001 cctgttcctg agcagtttga tattgagttt tccgttggtg agattgagcg cgccctgtac
     9061 gcaaaaatcg ttaaaaaatg cggtaaccgg catcactggg aagactgggc gaacgatatt
     9121 gctaaaattg cccgcacaca tatcgaccgc attcacggca ttctcgaaaa tccgggtaat
     9181 caagtagaaa tcagtgcctt cagggcattt gccgatgaac tgcgtgatga tctgaataac
     9241 agtgtttccg acgatgaaat cattgaaatg ctggcacagc atctgataac caagccggtc
     9301 tttgatgccc tgtttgccga ttacaatttt accgaccata acccgatgag tatggcgatg
     9361 cagaacgtgc ttaacacgtt acaggagcat catctcgaaa aagaagccag cacgctgaac
     9421 agcttctatg agtcggtaaa aatgcgtgca gacggcatta ccaccgcaga aggtaaacag
     9481 cagattatcg tacagttgta cgataagttt ttcagaaacg cgttcccgag aatgactgag
     9541 cgtctgggaa ttgtgtatac gcctgttgag gtggttgatt ttattattca cagcgtgaat
     9601 gacgtgctga aaggggtcag tttggagatc ggaaattttt gtacgttaag cacattattt
     9661 aaccaattcg gatacgtaag tgattgattt atcgtcattg gctatcgctt ttaggttgtc
     9721 gctcttgctg tatcgcattt aggttatccg attaagacaa aatcccctga aaacgctgta
     9781 tagcgcggga gtacacccta ttgctacagg taagcctgtt caaaaaacag gcttaccgta
     9841 caataattct ctatatccaa actgacccc
//
