LOCUS       NZ_AP026892             3701 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter asburiae strain KAM576 plasmid pKAM576_6, complete
            sequence.
ACCESSION   NZ_AP026892
VERSION     NZ_AP026892.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535934
            Assembly: GCF_025999975.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 54x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,017
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,783
            CDSs (with protein)               :: 4,783
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 47 of 118
            Pseudo Genes (incomplete)         :: 71 of 118
            Pseudo Genes (internal stop)      :: 20 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026892.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="KAM576"
                     /db_xref="taxon:61645"
                     /plasmid="pKAM576_6"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(3688..3701,1..487))
                     /locus_tag="OO861_RS25055"
     CDS             complement(join(3688..3701,1..487))
                     /locus_tag="OO861_RS25055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_264885052.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRLE
                     LNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGP
                     GGH"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(477..800)
                     /locus_tag="OO861_RS25060"
                     /old_locus_tag="KAM576c_48930"
     CDS             complement(477..800)
                     /locus_tag="OO861_RS25060"
                     /old_locus_tag="KAM576c_48930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_264885053.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARHVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2801..3016)
                     /locus_tag="OO861_RS25065"
                     /old_locus_tag="KAM576c_48950"
     CDS             complement(2801..3016)
                     /locus_tag="OO861_RS25065"
                     /old_locus_tag="KAM576c_48950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3023..3514)
                     /locus_tag="OO861_RS25070"
                     /old_locus_tag="KAM576c_48960"
     CDS             complement(3023..3514)
                     /locus_tag="OO861_RS25070"
                     /old_locus_tag="KAM576c_48960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          complement(916..1089)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          1013..1615
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.065"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..487)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.383"
                     /coverage="99.0"
                     /evalue="2.02e-101"
     Typing          complement(484..800)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.67e-59"
     Typing          complement(2710..3701)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.592"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtccggacgg cgtgaccagc gcccggcggt tttccggcgc gttcgggtca tgcagcccca
       61 gtctgccgtt cacgatggtc tgccaggcat cgatgcgcgg ccggtcggcg cggtcgtagt
      121 acggctggag ccgcctgccg gtcagcagtt cggtattggg gatcagaaag ttcagctcca
      181 gacgcccctt gtccgcgtgc tccacccaca gaatgctgta ctgatcttta tcgagtccgg
      241 gcatcagaac ccgctcgaag ctggccatca gtttttcccg ctggccggga ggcagatcgg
      301 cctccgcaaa cgacaggacg ccggaggtgt atttcttcac gtaaggcgac gcatcaatca
      361 gctcccggac ttcttccggc ttcccctgca ggacgctggc gccttcacgc tgccggtctt
      421 tacccagcag atagtccacc ggcccggcgc caccgccgcg accgcgtgga tgaaatttaa
      481 cgatcatcgt ccgttccttt ctcaagaacg gcgtgccgca gccgctcaag cccggcatcg
      541 atggccatca gcgccgccac aatctgtacc ctgtcatggc cggtgccgcc gccggcgtta
      601 acatggcggg ctatctggtt caggttattg cccataccgg caagctggcg cagcagcgcc
      661 ggcgagagtg aggggagctt tccggtgcgc gcaggctttt cgtccaggca ggtctgccgc
      721 atccacgccg ccagcggcct gccgtcgcag cgctcaagca gccgccggtg ctcgccctcc
      781 gtcacccaca tggtgagcat cttgttccgt ttctctgaca aaccggcctc ctgataacgc
      841 cgggacgggc gggaaagcct ttcccggtcg gcgcggggtt ggacaagccg caggcgcgtc
      901 agtggctttt ggcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt
      961 gtatactggc ttactatgtt ggcacggatg agggtgtcag tgaagtgctt catgtggcag
     1021 gagaaaaaag gcggcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     1081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggaaa tggcttacga
     1141 acggggcgga aatttcctgg aggatgccag gaagaagctt aacagggaag cgagaaggcc
     1201 acggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     1261 tcaaatcagt ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg
     1321 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     1381 ggccgcggtt tctcattcca cgcctgacac tcggttccgg gaaggcagtt cgctccaagc
     1441 tggactgtat gcacgaaccc cccgttcagt ccgactacca cgctctttcc ggtaactatc
     1501 aacttgagtc caacccgaaa aggcacgaca aatcgccatt ggcagtagcc actggtaaac
     1561 aggatgtgcg agagatgttg tgagagttct tgaagtggtg ccattactac ggctacactg
     1621 gaaggacaga tttggtgaca cgtctcgcac aagactgtta ccacggttca gaaacttgcc
     1681 ggtttactga accttcgaaa aaccaccttg ccgggtggtt ttttcgttta cagggcaaga
     1741 gattacgacg gtaacgacag gatcacaaga agatcctaac ctcgaaaaca ttgctcatat
     1801 ccacgtctgg atatcaacca cccccagtta ctgccaccac ttctgttccc cttgataaca
     1861 tatccttttg aaaagattac taatcaggca agatagtagg atgatgcaca atcacccaac
     1921 aaaccagcat aaccatttga ataaatagaa aaataaaaac ataggatatt agagtaatga
     1981 aatacatttg ctttgcagca gtaatgattt cccttacagg atgcgttaat tcaagtggcg
     2041 tattacccat ggggccagat acttattcca tatctgttca tgctgcacct gtaaggggac
     2101 aggctggagc aaaaaaagct gcgttaacag aagcgaacca atattgcctt aatcaaggca
     2161 agaccatcct tgtgaagaac atggatcact ctacgtttag ctcgcttcca ggggatgata
     2221 taagcgtcat attccaatgc ttatcggttg gcgatagtgg attacagcga cctaattatc
     2281 aaagtcctgc tgatgtaaac gtcaacgtta attaggcacc atttaccata acatccgttg
     2341 gccgcaccgc tgaaacgggg ttggctgcgt ttccggtgcc agacgcgaga aagtggcgac
     2401 agacataacg aaaaaacacc agaaacgcag ccgctgaata ttacactggt gcatagtcat
     2461 gcagccagat gcatggatgt taaagtcaca ctaaaatccg cgccaatgcc ttaaaaaccg
     2521 acaaatgctt ctccagccag tcccacagct ccgccgccag atacaggcag ttaagcaatg
     2581 attcaataag atttaacagc ttcatttccc gctcctgaat aagtcgtgac ctttttgatc
     2641 acgaacctat ttagcgggag aaataagagc ccctcttcgt gcgggttgtc agctgcctgg
     2701 atggtttaaa gtgtcggccc gcggtaggtt ttctgcggct ggcgctccag ctccagtgtt
     2761 ttctgatact cattctccct ctgccggagc tcgatggcat tcagttcccg gacaagcggc
     2821 gcagctgctc gcttaaactc agcacctgcg tgctcaagtc cgacacgcgc tcgttcagca
     2881 ccgcgttctc ccgttgcata agcccacaca tgctccgcca ttccgcgaag gcgctctccc
     2941 attcgtccag cctttgcgag tagtcctgct gtagctgctc taatgcgctc agcaactgct
     3001 tttccagctc tgtcattttc cgctactcct gtttcaggcg gatccatgtc gtcccgtcgt
     3061 aggggatggc ttcggtttgc atcccgggcg gcaggataag aaacctccgg ccgtcgctgc
     3121 tctcctgata gcgcactccc cacgtccggg cagtcatttt cgccagggta tcttcctgct
     3181 gacgaagatg cgtgtaattg tcggttatct gctgcccctg ccaccacagt atgctcccgc
     3241 tcgtgccgat cagcagcgct gacaccatca ggatggtgaa ccatgtccgc ccggcagtcc
     3301 gcagtacgct ccgccggttt ttctccatcg cctcgctcat actctgctcg tgtgcgagga
     3361 tggcgtcgct gattctcctc gcgcttgcgc tcagttccgc tctgacagac tgctcgtgct
     3421 cgctgaatgc ggctttcagc atctcgccgg tactctgctg ctgcgctttc gattgctgct
     3481 ctaagtcctt cgccagcgtt aaaagactgc tcatagatgg ctcccttcag tcggatgttt
     3541 cgccccccgt ccgggtcggc aatgctgata ctgcttttcg tggtgcgcac cacctcaaaa
     3601 cctgaccgtt ccagcgcctc agtgacgtcc tgacggtttt tcagctcccc ggacgaagca
     3661 agggtcagca gtccgcgcgt gatggcttca gtggcctcct g
//
