LOCUS       NZ_AP026891             3944 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter asburiae strain KAM576 plasmid pKAM576_5, complete
            sequence.
ACCESSION   NZ_AP026891
VERSION     NZ_AP026891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535934
            Assembly: GCF_025999975.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 54x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,017
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,783
            CDSs (with protein)               :: 4,783
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 47 of 118
            Pseudo Genes (incomplete)         :: 71 of 118
            Pseudo Genes (internal stop)      :: 20 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026891.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="KAM576"
                     /db_xref="taxon:61645"
                     /plasmid="pKAM576_5"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(3097..3944,1..43)
                     /locus_tag="OO861_RS25020"
     CDS             join(3097..3944,1..43)
                     /locus_tag="OO861_RS25020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_264885051.1"
                     /translation="MHNAALELFNDRLPHKPYFSDDLYFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRIGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPNGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWKIAVWQPELYTLDWLADSLDLNAA
                     NDKEIVAEYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLEACYERANAYNLQFSSPLD
                     EKEVGGIAKSIAKWTHHQLSPEGFAEYVSRTHAPSIQAVRGRKSRGGGRPKDSSALVNT
                     KPAAQLSIASREVSF"
                     /name="replication initiation protein"
     gene            710..1036
                     /gene="mobC"
                     /locus_tag="OO861_RS25025"
     CDS             710..1036
                     /gene="mobC"
                     /locus_tag="OO861_RS25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_202218207.1"
                     /translation="MEKRTREIKIRLTDSEHQRLIDRCDRTHLAEWLRQLGLGEHTARK
                     RRLPDVAPELLRQVSGIGNNINQIARRLNQLDGLTPTERVSLLAVLNSLDRNLGELLEQ
                     HRDH"
                     /name="mobC"
     gene            complement(1044..1316)
                     /locus_tag="OO861_RS25030"
                     /old_locus_tag="KAM576c_48900"
     CDS             complement(1044..1316)
                     /locus_tag="OO861_RS25030"
                     /old_locus_tag="KAM576c_48900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_262130275.1"
                     /translation="MLNPQAGVLVFVETGHQCLFPFVHQPRLFRLCNVGLLKGQHAGGV
                     GAGKVTAVNQFAGAFGVSPQHTGIIPLAIFSQQVIDGATARTGTA"
                     /name="hypothetical protein"
     gene            1245..>1664
                     /locus_tag="OO861_RS25035"
                     /pseudo=""
     CDS             1245..>1664
                     /locus_tag="OO861_RS25035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1714..2199
                     /locus_tag="OO861_RS25040"
                     /old_locus_tag="KAM576c_48910"
     CDS             1714..2199
                     /locus_tag="OO861_RS25040"
                     /old_locus_tag="KAM576c_48910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_202218204.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFGRLETSISEDLKLSS
                     QSISNAIREQNGRLTGMIRATATTALIWIVVCAALLIGILSGIIWFQGQIISSRLSDID
                     GYSQQLTSLKAKSGAGVTIYSDDSQKNGYLVVLPVKAHGVESYTSTAGNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            2260..2493
                     /locus_tag="OO861_RS25045"
                     /old_locus_tag="KAM576c_48920"
     CDS             2260..2493
                     /locus_tag="OO861_RS25045"
                     /old_locus_tag="KAM576c_48920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_202218208.1"
                     /translation="MTEMEKQLLSALESLQASYEQQHQAWQESYSSLQHMFETTSQALT
                     HSDRVCQHLSSQVDTLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2683..2919
                     /locus_tag="OO861_RS25050"
     CDS             2683..2919
                     /locus_tag="OO861_RS25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010566328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202218205.1"
                     /translation="MAKSAQAIAKEINDLMRKNGNECITLKWGQFYDICERERLADVVM
                     GKVAESLKKNDLHIIYGNNVIIVRDFCWNPVSL"
                     /name="hypothetical protein"
     Typing          complement(844..1036)
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.237"
                     /coverage="100.0"
                     /evalue="1.78e-68"
     Typing          1027..2585
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.192"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agccgcgcaa ctttctatcg caagcaggga ggttagtttt taaactctcg agctatagcg
       61 aacaaagtca ctaaaatcgc aaaaatcact attaagttca tgacaggtaa ggagcaaaga
      121 gtcatgacca actgtaaaag tagatttcca acgattcttg caatgataaa actacaaacg
      181 taatcactaa tcaaatcaaa catatatatc tccacgaagg agagtccatg cgataaatat
      241 ccaaggggga acgtatccct tgtgtccgaa ctatgcttga cgttttttac ctatttgcta
      301 atatacgttt accaagacga taaaagctaa agatacaaaa caagaaacga gcccttcatt
      361 taaccataaa aaccccaaaa agttaaagtc ccagttataa caataacaca tattttcggt
      421 gaagcaattt ttttttgcac ttttcgcaaa aaaaattatc cacccccttt ttatgcgtga
      481 gacaaaaaca acctatatca gataacagcc cttctttttc agggtggggg ttttggcagc
      541 gaccacagaa tctgacatac tccaccatac tttaagccag tacacacagc gtcccctcta
      601 cgaggtgccg tctgtgactg gttcaggggg ctcgccgccc cccgaaaccc cgggcatcca
      661 ctgcgaaact ttcgcacttt gggtgcgaat atttctcagc ggagtgagaa tggaaaagcg
      721 tacgagagag atcaaaatcc gcctgaccga ctcagaacat cagcgtctga tcgaccggtg
      781 tgaccgaacc catcttgctg aatggctgcg tcagctcggt ctgggagaac acaccgccag
      841 aaagcgccgt ctgcctgatg ttgcgcccga actgctgcga caggtcagcg gtatagggaa
      901 caacatcaac cagatcgccc gccgccttaa ccagcttgac gggcttactc ccacggagcg
      961 ggtttccctg ctggcggtgc tcaacagcct cgaccgcaat ctgggtgaac tgctggagca
     1021 gcaccgtgat cattaaaatt cactcacgcg gtgccggttc gggcagtggc cccgtcgatt
     1081 acctgctggg aaaagatcgc cagcgggatg atgcccgtgt gctgcgggga gacgccgaac
     1141 gcacccgcga actgattgac ggctgtgact ttgcccgcgc ctacacctcc ggcgtgctgt
     1201 cctttcagga ggccgacatt gcagagcctg aaaaggcgcg gctgatggac gaatgggaac
     1261 agacactgat gaccggtctc gacaaagacc agtacgcctg cctgtgggtt gagcatcagg
     1321 acaaaggcag gctcgagctg aacttcgtga tcccgaacat cgaattacag agcgggaaac
     1381 ggctgcaacc ctactttgac cgcgccgacc gtcccagggt aaacgcctgg cagacgctga
     1441 ccaacgaccg gctcggactg cgcgacccca atgaccccgc ctaccgccgc ccgctgacgc
     1501 aggccagcga cctgccgcga gacaaacagc aggcagcgga aaaactgacc gccggcctga
     1561 tgaacctgat ggcacagggc gtaatacgca gccggcagga cgtggtgact cagatcgaga
     1621 gcattggtct caccgtggcg cgggaaacca gaagcagcat cagtattgcc gacccgtccg
     1681 gcggcagaaa catccggctg aaaggaatga tttatgagcg agattttaaa tttggcgaag
     1741 ggcttcgagg agaaatcgaa gcagcaagcg caggataccg agcagagcgt gaagcgcgag
     1801 ttcgggaggc tggaaacgtc tatcagcgag gacttgaaat taagctcgca gagcatcagc
     1861 aacgctatcc gcgaacagaa cggcaggctg acgggcatga tcagagccac ggcgaccacc
     1921 gcgctgatct ggatagtggt ctgcgccgcg ctgctgattg gtatcctgag cgggataatc
     1981 tggtttcagg ggcagatcat cagctcacgc ctgagcgaca tcgatggcta cagccagcaa
     2041 ctgacgagcc tgaaagcgaa aagcggcgcg ggggtgacga tctacagcga cgacagtcag
     2101 aagaacggtt atctggtggt gctgccggtg aaagcgcacg gcgtggagag ctacacctca
     2161 acggcaggca acacggtggt gaagtacacc gcgaaataac cccgccagaa cgcacagaaa
     2221 cgcccctgaa cgattttgac ccacaggaca gagaaaacca tgaccgaaat ggaaaaacag
     2281 cttctgagcg ccttagagag cttacaggcc agctacgagc aacagcatca ggcgtggcag
     2341 gaaagctaca gcagcttgca gcacatgttc gagactacct cacaggcgct gacgcacagc
     2401 gacagggtgt gtcagcactt gagcagtcag gtggacacct tgcgcgcgca ggtcgagagc
     2461 ttgagcagga acgtcagccg cttgatgcgc tgattatgca gcatgaccgc gtccgtgcag
     2521 ataagcagcg agagcagcag ccggaaccgc agaaaaagcc tactcgctat cacggcccct
     2581 cgatgtgatg tttttagtct tctttagtgt tatttttaga ttgctctaag gtttttttag
     2641 tgtaatctca gcgtcacttt aatttgtttt ggaaaataaa aaatggctaa gagtgcgcaa
     2701 gcaatagcta aagaaataaa tgatttaatg agaaaaaatg gaaatgagtg tattacttta
     2761 aagtgggggc agttttacga tatctgtgag agagaaaggc ttgccgatgt tgttatgggg
     2821 aaggtggctg aaagcctgaa gaaaaacgac cttcatatca tctacggcaa caacgtgatt
     2881 atcgtcagag acttctgttg gaatcccgta tctctttaga gactaatgac aatctgctgt
     2941 cttaaggtgt aagtttaacg gcagaaaaaa cgaagccccg gcatcatgta taatcttggc
     3001 ggatccgcat gataaccagg gctaggtacg ataccttgaa aggatattag cacatgaacc
     3061 gtgccagaca acagcccgcc ataagcgcag ggcgcattgc ataacgctgc actcgagctc
     3121 ttcaacgacc gcttaccgca caagccgtat ttttcggacg acctgtactt tggtgtacgt
     3181 attgccggta aagaacgcgc cattctggcg aaatacatcc agttcaatca gcctcacgcc
     3241 atgttctggc tgggattcga cgtcgatcgc atcggtgccg ccatcgactg gagcgaccgc
     3301 aacgcacccg cgcccacgct aactattacc aatcccgaaa acggacacgc gcatctgctt
     3361 tacgcgctca aaacctctat acgtaccgca ccgaacggga aaatgaagcc gctgcgttac
     3421 gctgctgcgg tggagaatgc gttacgtaaa aaattagaag ctgatgcggg gtattcgggg
     3481 ctaatctgta aaaatccgaa ccacagccac tggaaaatcg ctgtatggca gccggaactc
     3541 tacacgctgg actggcttgc tgactcgcta gacctgaacg ctgcgaatga taaagaaatc
     3601 gtcgcagaat atggcttagg ccgtaactgc actctgttcg acaaaacccg caagtgggca
     3661 taccgcgcaa tacgtcaggg ctggcctgag tatgagcagt ggctggaagc ctgctatgag
     3721 cgtgccaacg catataatct gcaattctca tcacctcttg atgaaaaaga ggtgggaggt
     3781 atagcaaaaa gcattgctaa atggacacat caccaactat cccctgaagg atttgcggag
     3841 tatgtatcac gcacacacgc accatctatt caggctgtta gaggccggaa aagcagaggc
     3901 gggggcaggc caaaggacag ttcagccctc gtaaacacga agcc
//
