LOCUS       NZ_AP026890             4912 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter asburiae strain KAM576 plasmid pKAM576_4, complete
            sequence.
ACCESSION   NZ_AP026890
VERSION     NZ_AP026890.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535934
            Assembly: GCF_025999975.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 54x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,017
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,783
            CDSs (with protein)               :: 4,783
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 47 of 118
            Pseudo Genes (incomplete)         :: 71 of 118
            Pseudo Genes (internal stop)      :: 20 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026890.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="KAM576"
                     /db_xref="taxon:61645"
                     /plasmid="pKAM576_4"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(4512..4912,1..877)
                     /locus_tag="OO861_RS24995"
                     /old_locus_tag="KAM576c_48840"
     CDS             join(4512..4912,1..877)
                     /locus_tag="OO861_RS24995"
                     /old_locus_tag="KAM576c_48840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_264885049.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVINRSMAIITFNPEGIVLEANENFVNATGY
                     KRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRATYNP
                     VFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENSILM
                     IDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGASGRS
                     FAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMREAGE
                     VMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            1884..2207
                     /locus_tag="OO861_RS25000"
                     /old_locus_tag="KAM576c_48850"
     CDS             1884..2207
                     /locus_tag="OO861_RS25000"
                     /old_locus_tag="KAM576c_48850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2416..>2850
                     /locus_tag="OO861_RS25005"
                     /pseudo=""
     CDS             2416..>2850
                     /locus_tag="OO861_RS25005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2885..3376
                     /locus_tag="OO861_RS25010"
                     /old_locus_tag="KAM576c_48860"
     CDS             2885..3376
                     /locus_tag="OO861_RS25010"
                     /old_locus_tag="KAM576c_48860"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3383..3598
                     /locus_tag="OO861_RS25015"
                     /old_locus_tag="KAM576c_48870"
     CDS             3383..3598
                     /locus_tag="OO861_RS25015"
                     /old_locus_tag="KAM576c_48870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1599..1771
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="1.98e-82"
     Typing          complement(1128..1674)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2198..3696
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cataaaccgt tctatggcca tcatcacctt caatcctgaa gggatcgtgc ttgaagcaaa
       61 tgaaaatttc gtcaatgcca ctggctataa gcgggatgaa atcataggta agcatcatcg
      121 tctgttctgt gccgagacgc tttacaaaag tgatgaatac cgacatttct gggagagtct
      181 gaatcaaggt gagttttttt ctggtctgtt tccacgcctt aaccgtcagg gagaccctct
      241 gtggttccgt gcaacttata accctgtctt taacagtgat gggcagcttt ataaaattgt
      301 gaaattcgct acagatgtta cggctgatgt cctgcgaaac cagagagagc aagaggctgc
      361 tgtgcatgca tgggatatgg ccgtgcagac ccgagaaagt gcgcagaacg gtgccaatgt
      421 tattgaaaat agcatcctta tgattgacag aattgcgcag gggatggggg ctgtctccac
      481 cgatatctca cggctcaaca atcagtctga aagtattgac gatatggtgg aaaccatccg
      541 gaagtttgcc atgcagacaa gacttattgc actgaatgcc gcgattgaag cagcaagggc
      601 tggcgcatcc ggaagaagtt ttgcggttgt tgccgcagaa gtgcgtaatc ttgcggcgag
      661 cgtcagtagc gcgactgaag aaattgagca agtcgtggcc agcaatagtc aactggccaa
      721 ggatgttctc tgtggcattg aaaacagcct gatgaacact cgggaagggg ttacactcat
      781 gcgcgaagcg ggtgaagtta tgaccagcat tcagaggaat gctgcagggg ttgagactgc
      841 ggttaaggat gtcgccattt cagttaaagc cggataggcc atggtgcata tccccatatc
      901 tgtcgaacat cacagatgat cttcttgaga tcctttccgt tgcgtcgtaa tctcttgctc
      961 tgaaaacgaa aaaaccaccc ggcaaggtgg ctttttcgaa ggttcgcaag agttgacgct
     1021 cttttgaacc gcggtaactg gcttggagga gcacagtcac caaaactgtc ctttcagtgt
     1081 agccttatcg cacccgaaac ttcaagactc tcctcctcta aacctgttac cagtggctgc
     1141 tgccagtggc gctttgtcgt gtttttccgg attggactca agttgatagt taccggataa
     1201 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     1261 cttcccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
     1321 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg
     1381 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
     1441 gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgtggcc ttatcgcttc
     1501 cctgttaagc ttctttctgg catctcccag gaaatttccg ccccgcctgt aagccggtac
     1561 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaatatat
     1621 cctgtatcac atattctgct gacgctccgc tgctgcattt ttctcctgcc acatgaagca
     1681 cttcactgat attcgcatcc gtgccaatat agtaagccag tatacactcc gctagcgcta
     1741 cgtgactggt tcagggctgc gccccgaaac ccgccaaaag cctgacgcgc ctgcggcttg
     1801 tccaaccccg cgccgaccgg gaaaggcttt cccgcccgtc ccggcgttat caggaggccg
     1861 gtttgtcaga gaaacggaac aagatgctca ccatgtgggt gacggaggac gagcaccggc
     1921 ggctgcttga gcgctgtgac ggcaggcagc tggcggcgtg gatgcggcag acctgcctgg
     1981 acgaaaaacc tgcgcgcacc ggaaagctcc cctcaatctc gccggcgctg ctgcgccagc
     2041 ttgccggcat ggggaataac ctgaaccaga tagcccgccg tgttaacgcc ggcggcggca
     2101 ccggccatga cagggtgcag attgtggcgg cgctgatggc catcgatgcc gggcttgagc
     2161 ggctgcggca cgccgttctt gagaaaggaa cggacgatga tcgttaaatt tcatccacgt
     2221 ggccgcggcg gtggcgccgg gccggtggac tatctgctgg gtaaagaccg gcagcgtgaa
     2281 ggcgccagcg tcctgcaggg gaagccggaa gaagtccggg agctgattga cgcgtcgcct
     2341 tacgtgaaga aatacacctc cggcgtcctg tcgtttgcgg aggccgatct gcctcccggc
     2401 cagcgggaaa aactgatggc cagcttcgag cgggttctga tgcccggact tgataaagat
     2461 cagtacagca ttctgtgggt ggagcacgag gacaaggggc gtctggagct gaactttctg
     2521 atccccaata ccgagctgct gaccggcagg cggctccagc cgtactacga ccgcgccgac
     2581 cggccgcgta tcgatgcctg gcagaccatc gtgaacggca gactggggct gcacgatccg
     2641 aacgcgccgg aaaaccgccg ggcgctggtc acgccggccg gactgccgaa aacgaagcag
     2701 gaagccgcag aagccatcac ccgtggactg ctgagcttag cctcgtccgg ggagctgaaa
     2761 agccgtcagg acgtcactga ggctcttgaa ggtgcaggtt ttgaggtggt gcgtacaacg
     2821 aaaagcagta tcagcattgc cgaccctgac ggggggcgaa acatccgact gaagggagcc
     2881 atctatgagc agtcttttaa cgctggcgaa ggacttagag cagcaatcga aagcgcagca
     2941 gaagagtacc ggcgagatgc tgaaagccgc attcagcgag cacgagcagt ctgtcagagc
     3001 ggaactgaac gcaagcgcga ggagaatcag cgacgccatc agcgcccacg agcagagtat
     3061 gagcgaggcg atggagaaaa accggcggag cgtgttgctg acggccggga ggacatggct
     3121 caccatcctg atggtgtcag cgctgctgat cgccacgagc gggagcattc tgtggtggca
     3181 ggggcagcag ataaccgaca attacacgca tcttcgtcag caggaagata ccctggcgaa
     3241 aatgaccgcc cggacgtggg gagtgcgcta tcaggagagc agcgacggcc ggaggtttct
     3301 tatcctgccg ccggggatgc aagccgaagc catcccctac gacgggacga catggatccg
     3361 cctgaaacag gagtagcgga aaatgacaga gctggaaaag cagttgctga gcgtattaga
     3421 gcagctacag caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg
     3481 gcggagcatg tgtggtctta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga
     3541 cttgagcacg caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggaactgaat
     3601 gccatcgagg agaaggctgc actggagtat cagcgtcaga acgagctgga gctgacgctc
     3661 cgacagaagc agaagagttt cgatggtccg aagatgaggt gacagcccgc acgaagaagg
     3721 gctcatcgcg ttcctgctaa ataggctgac taccggaatg gtagtgactt atcccggagg
     3781 aagtgatgaa aattctcaac gttattgaag ccctgctgaa ctgtttttac ctggtgatgg
     3841 agctgtgtga gttgccggag aggcatttgc cggtttttaa ggcgttcgcg ctgattttag
     3901 tgtgacttta cgctgcatgc atctggctgc atgactatgc atcagtgtaa tatccagcgg
     3961 ctgcgttttt ggtgtttttt cgtcatgtct gtcgccactt tctcgcgtct ggcaccggaa
     4021 acgcagccaa ccccgtttct gcggtgcggg caaggggtgt tatgttaaaa gccgaggtct
     4081 ggcggatacg gaaaaaatta tgcccgcggc aatccggccg atacttaaat tatccttcac
     4141 aaaggatgtg ctgcgatcca ggcagttgag tgttttccgg ctcccggatg ggagccattt
     4201 ttttgccttc tgatgcctgt taatgcatgc ggagctgcgc tggttcttcc aagattgata
     4261 gacgccagct tctctgctgg agccggcgga tttgccgtaa atatgaacat tttcttattg
     4321 ttcagcccgc taaagatggc gggtttttta cgcgtgaaat ccggagccgg acatcatgcc
     4381 ggcatggctg aatagcgggc tttttcccgc cctgtcgcag taatatataa cgctacataa
     4441 ggagtagccg ataaccatgg cagtacttac attatccggg caatctgatt catatgtttt
     4501 cacttaaaaa tatgttctcc ctgaacctcc agcgatcagc catttcgtat gacgcaattt
     4561 gtcagaataa gcctgtcatt gagttcagtc cggagggggt gataaataaa gccagcccgc
     4621 tttttctctc cactatgggg tacagggctg atgaaataat cggtcaccac catcgtatat
     4681 tctgcccgcc ctcgctggta agttcaccgg aatacagcca gttctggcag cgccttgcca
     4741 ggggagaaag ctttagtggc aagtacctgc ggctggcaaa gggaaatcgt tccgtatggc
     4801 ttgaagccag ttatatcccc gtttctgaca ggcgcggccg tgttatcaga gtcattaaaa
     4861 ttgctgccga tatttctgag cgcgtgcatt ctgctcttga gcaggaagca gt
//
