LOCUS       NZ_AP026889             5137 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter asburiae strain KAM576 plasmid pKAM576_3, complete
            sequence.
ACCESSION   NZ_AP026889
VERSION     NZ_AP026889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535934
            Assembly: GCF_025999975.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 54x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,017
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,783
            CDSs (with protein)               :: 4,783
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 47 of 118
            Pseudo Genes (incomplete)         :: 71 of 118
            Pseudo Genes (internal stop)      :: 20 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026889.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="KAM576"
                     /db_xref="taxon:61645"
                     /plasmid="pKAM576_3"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(4456..5137,1..365))
                     /locus_tag="OO861_RS24970"
     CDS             complement(join(4456..5137,1..365))
                     /locus_tag="OO861_RS24970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_264885045.1"
                     /translation="MAIFHLSFKILTRTTKTGKSKSSLYLAAYNSREKLKDELTGLTFD
                     YTKKTDLYKSGIILPEDAPAKFYDRATLWQNCEAAESRRDSQICRYFIVSLPREQNGEQ
                     NEELLKKYLKNNFVKLGIINNKPHAHVMLTMRKCENDGFSKKKAREWNNKELAEVWRRQ
                     WAVLVNKTLRENGHKNTITHLSFLRQKELAIAKAREALENNDIKKAEELTTLAKHLATK
                     KPVKRKPTIKYLKDKARKKNKNTELRERLRQQQKQKEETNKKESKLNLIFKNLILRCKR
                     SKKTEITKMTEADFLKNMKTDEKEAEDLELNKYIDKELENELNKKNDITHTPTRDRVRN
                     RTQKGYKS"
                     /name="MobA/MobL family protein"
     gene            740..1108
                     /locus_tag="OO861_RS24975"
                     /old_locus_tag="KAM576c_48770"
     CDS             740..1108
                     /locus_tag="OO861_RS24975"
                     /old_locus_tag="KAM576c_48770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148731312.1"
                     /translation="MGKSEIDILAEELKEFYFDALGENNGRVFAYRATYKVKGWEQIEQ
                     KAFRNAFFKFFKTDAQLRKTKDIKSDYFQLEGIKDKFNRYYFPSFCIDKKEYEARGVEY
                     LKEVEEYFKKLITLAAIK"
                     /name="hypothetical protein"
     gene            1229..1525
                     /locus_tag="OO861_RS24980"
                     /old_locus_tag="KAM576c_48790"
     CDS             1229..1525
                     /locus_tag="OO861_RS24980"
                     /old_locus_tag="KAM576c_48790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_099456243.1"
                     /translation="MSLQQQKERLKRELAALEKKEKHLKDQANAKNMKAIAAEILKYDL
                     EGYDNFKKVVGLLAYFDYQDDDFKNGLMKWAENELEERKAKKVARTFKKKPQK"
                     /name="hypothetical protein"
     gene            1547..2092
                     /locus_tag="OO861_RS24985"
                     /old_locus_tag="KAM576c_48800"
     CDS             1547..2092
                     /locus_tag="OO861_RS24985"
                     /old_locus_tag="KAM576c_48800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264885047.1"
                     /translation="MDKLTLLDMFKHGTATWSAVSSFVVVLFWCVGCWLAFLAIVNYKN
                     AADGKSGIAKPIVQTVIASLMVAISRFIPILSDTLNNKAAEFSPQSLLSDIPTTDPMGL
                     GLAFTSVLLFVQMLGTIAIFRGFLMIWEATNKGAGSGLVGKSWTHIIGGVLAVNIQLTI
                     STVAATFYPGVDLSFLGL"
                     /name="hypothetical protein"
     gene            2828..4306
                     /locus_tag="OO861_RS24990"
                     /old_locus_tag="KAM576c_48810"
     CDS             2828..4306
                     /locus_tag="OO861_RS24990"
                     /old_locus_tag="KAM576c_48810"
                     /inference="COORDINATES: protein motif:HMM:NF013603.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_264885044.1"
                     /translation="MLYKNNKAYADNVNKAGTPFLSVYATQNGERLNERKGKSIRLAKL
                     YYLQAEKLEGGKAEAMKKRAGRVFGCSSHMEMAIGSGGYGYSLYSHRCKDRSCTTCQRV
                     RAYILQSKVREITPKLIEQTNAKDGLIFGTLTIKNPPIVELKDYLKIMSKAFARMFKRK
                     EFKIGVGGFRCFEVTRGESGADFCHPHIHFLLQVKASYFARNSPLYINADQWATAWTDC
                     LKLEVEKTGRVFNVADYPNGKAFVKILRVQAPDYTRENRKYATIETLQKDGDQVINYVL
                     KYTAKEDEGSKKALVKNDDWFFEYDKQIKNIRAISFFGIYKTLISELPPREYCEKEIKT
                     ELNNNQAKFYSVIWDDDHKYIAIETTEEEALNKKRTNTINSIKTTLIAQLESKNYILDI
                     MIAAMKKGDFLTVNKEINNHNILADRTYKTFKRMEKAGELEERKSGFYQPFHDYLFKSV
                     IYEKMESILTAEELENIKREIEINIEVVEDNPPF"
                     /name="protein rep"
     Typing          complement(4..365)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.124"
                     /coverage="98.0"
                     /evalue="5.97e-53"
     Typing          complement(4460..5136)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.952"
                     /coverage="98.0"
                     /evalue="9.19e-93"
ORIGIN
        1 attcctaatt ttacaaaatt attttttaaa tattttttta atagctcctc gttttgttcg
       61 ccgttttgtt cgcgtggcaa ggatacaata aaatatcgac agatctgact gtcccggcgc
      121 gattctgcgg cctcgcaatt ttgccataat gtagcccggt catagaattt ggcaggggca
      181 tcttctggca aaataattcc gcttttatat aaatctgttt tttttgtata gtcaaatgtt
      241 aagcctgtta attcgtcttt aagcttttct ctactgttat aagcagcaag ataaagcgag
      301 cttttgcttt ttcctgtttt agttgttctt gtcaaaattt taaaactcaa atgaaatatt
      361 gccacggctg gcctcctttt gtttttgttt ttttatgccc acacacacac ggaaggggag
      421 ggagggcata ggcgggggca accattgcga agctttggga agccttgcgc cgtgcggtgc
      481 gcgttagcgt tccgcttgtc gcttgcgaca aagggcgaag cccttaagcc tcgcagagac
      541 cgcacgttgc cgataggcaa tgtataagcg ggcatttatc actaacgttc taaatgtggc
      601 ttacttcgtg cgcagctacc gcctttttta ttatttaata aaacctttta aaaaaagtaa
      661 ataaaaccaa ttaaaaattt gacatttatt ttttatatat taaagaaaaa atataaaaga
      721 aaaaagaggg gtttaattta tgggaaaatc agaaattgat atattggcag aagaattgaa
      781 agaattttat tttgatgcgc tgggtgaaaa taacggtcgt gtttttgctt atcgagcaac
      841 ctataaggtg aaagggtggg agcaaataga acaaaaagct tttagaaatg cgttttttaa
      901 atttttcaaa acagatgctc aattaagaaa aacaaaagat ataaaaagcg attattttca
      961 actggaaggg ataaaggata aatttaatag atattatttc ccttcatttt gcatcgataa
     1021 aaaagaatat gaggcaagag gtgttgagta tttaaaagag gttgaggaat actttaaaaa
     1081 attaataacc ttggcggcta taaaatgatt ataactttta ttctaaatat tatcgttatt
     1141 gtttcattat ctgcgcttta tattataagg cgcagggaag aattaaaaaa aaaagaaaaa
     1201 gcaaaacatt ataaaataag ggggctatat gagcttacaa caacaaaaag agagattaaa
     1261 aagagaactc gcggcgttag agaaaaaaga aaaacactta aaagaccagg cgaacgctaa
     1321 aaatatgaaa gcaatcgcgg cggaaatttt aaaatatgat ttagaaggat acgacaattt
     1381 taaaaaagtt gtcggattgt tggcatattt tgattaccaa gacgacgatt ttaaaaacgg
     1441 cttgatgaaa tgggcagaaa acgaacttga agaaagaaaa gctaaaaaag tagctcggac
     1501 atttaaaaaa aaaccacaaa aataataaat aatgaggggg ttaatcatgg ataaattaac
     1561 cctgttggat atgttcaaac acggaaccgc tacatggtca gcggtttcgt ctttcgtcgt
     1621 tgttttgttt tggtgtgttg gctgttggct ggcatttttg gcgattgtta attataaaaa
     1681 cgcagccgat ggaaaaagcg gaatcgcaaa gccaattgtt caaactgtga tcgcatcttt
     1741 gatggtcgca atttctcgat ttattccaat tctttcagac actttaaaca ataaagcggc
     1801 tgagttttca cctcagagct tacttagtga tattccaacc actgatccaa tgggattggg
     1861 gctggcgttc acaagcgttt tattgtttgt tcaaatgtta ggcaccatcg ccatttttcg
     1921 gggcttcttg atgatatggg aagcaacgaa caagggcgca ggtagcggtc ttgttggtaa
     1981 aagctggacg cacattatag gtggcgtttt agctgtaaac atacagctta caatctcgac
     2041 ggtagccgct acgttttacc caggcgttga tctgtcgttt ttgggtcttt gagtgtaggc
     2101 gggtcaatgg ccagcttgct gggcgtagcg aaccattgac gcgacggaac gaccccaccc
     2161 ttggctaagg gggaaggaga gccacggcga gccttcctcc tgttaagccg cccttttgcc
     2221 ctttatactc accgcaggac ggcaggcgtc cgggtgggtt tgtttgtttc gtgtctgtcg
     2281 tcctatccct tccctttctc tctggatctg gcagtcgcag ggtttttgtc ttttagcccc
     2341 ctctgggggt tgggggttta aaatccgttc aggatttgcc ttttgctttt gacctttctt
     2401 ttaaagcccc ataggggcgc actcaacttg attattaaga aataaatcgc atcgtttttg
     2461 gggggtaaat cgccctgtaa gccgcgccat tgctggattt cttgattttt ttaattacat
     2521 accaaaacga gaaaaagcgc aaaaattaca caacttaaaa aggggctaaa agaaacgata
     2581 aacgggcaac ataaagcgat aaggggtaaa cctgggacag ctctacattt ttagccctaa
     2641 atgtgggtgt ttttgggacg gcatacatta aagcagttaa aaagcgggct ataaatggga
     2701 cgggatcaac cgttgggggg atttgcgcgg gtgtttttgt ttatggcgat tttttaagca
     2761 tttgacaaaa aagaaaaatc ccgtaacttt tgaagtgcga agacaaaaga aaacgggatt
     2821 gatcagtatg ctttataaaa ataataaagc atacgcggat aatgtcaaca aggctggaac
     2881 gcctttttta tcagtgtatg cgacccagaa tggggaaagg ttaaacgaaa gaaagggcaa
     2941 gagtataaga cttgccaaac tctactacct ccaagctgaa aagctcgagg gcggtaaagc
     3001 tgaggctatg aagaaacgcg cggggcgtgt ttttgggtgt tccagccaca tggagatggc
     3061 catagggtcg ggtggatatg gctattcgct ttatagccac cgttgcaaag atcggtcgtg
     3121 caccacttgc caacgcgtcc gtgcgtacat actacaaagt aaagtgcgcg aaattacgcc
     3181 aaagctaata gagcaaacaa acgcaaaaga tgggctaatt ttcggcacgt taactataaa
     3241 aaacccgcca attgttgaat taaaagacta tttaaaaata atgagtaaag catttgctcg
     3301 tatgtttaaa agaaaagagt ttaaaatagg tgtaggcggt tttaggtgtt ttgaagtaac
     3361 acgcggcgaa tcaggcgcgg atttttgcca tccacatatt cattttttac tacaagttaa
     3421 agcgtcatat tttgcgcgta attctccatt atatataaat gctgatcagt gggcgacagc
     3481 atggactgac tgtttaaaat tagaagtaga aaaaacgggg cgcgttttta acgttgcaga
     3541 ctatccgaac ggtaaagcat ttgtaaaaat tttacgcgtg caagcccctg attatacaag
     3601 agaaaatcgc aaatatgcga caattgaaac tcttcaaaaa gatggtgatc aggttataaa
     3661 ctacgtgctt aaatataccg caaaagaaga tgaagggtcg aaaaaagcat tggttaaaaa
     3721 tgatgattgg ttttttgaat atgacaaaca aataaaaaac atacgcgcta tttcattttt
     3781 tggaatttat aaaacattaa tatcagaact gccgccccgc gaatattgcg aaaaagaaat
     3841 aaaaacagaa ttaaacaata atcaagctaa attttacagc gtcatctggg atgacgatca
     3901 taaatatatc gctattgaaa cgacagagga agaagcttta aacaagaaaa gaacgaacac
     3961 aataaacagc ataaaaacaa cattgatcgc acagcttgaa agtaaaaatt atattctgga
     4021 tataatgatc gcggcgatga aaaaaggcga ttttttaact gttaataaag aaataaacaa
     4081 tcacaatatc cttgccgacc gcacatataa aacatttaaa cgaatggaaa aagcgggtga
     4141 attggaggaa aggaaaagcg gattttatca gcctttccat gattatttat ttaaaagcgt
     4201 tatttatgaa aaaatggaaa gcattttaac ggcggaagaa ttagaaaata taaaaagaga
     4261 aattgagata aatatagaag tagtggaaga taacccacca ttttaatata aataattaag
     4321 ccccgtatat ggggctttta ttacctgtta ttttttatct gtttagcgtt atattcttct
     4381 tcttttttgt tttttaattc ttcctctgga tctcttagtg tcatttcaat aatccacctt
     4441 ttcccgtttt cgtctctatg acttataacc cttttgcgtc ctgtttctaa ctcgatccct
     4501 cgtgggtgtg tgtgtaatgt catttttctt attaagctca ttctctaact ccttatctat
     4561 atatttattt aattctaaat cttcggcctc tttttcgtct gttttcatat tttttaaaaa
     4621 atcggcttct gtcatttttg ttatttctgt ttttttgctt ctcttacatc ttaatattaa
     4681 atttttaaaa atcaaattta acttactttc ttttttgttt gtttcttctt tttgtttttg
     4741 ttgttgtctg agtctttccc tcaattcggt atttttattt tttttacgcg ctttatcttt
     4801 taaatatttt attgttggct tgcgcttaac gggcttttta gttgctaaat gtttagccaa
     4861 tgttgttaat tcttcggctt ttttaatatc gttattttct aatgcttcgc gtgcttttgc
     4921 tattgccagc tctttttgtc gtaaaaaact taaatgtgtt attgtgtttt tgtgtccgtt
     4981 ttctcttaat gtcttattta ccaaaaccgc ccactgcctg cgccaaacct ccgccaattc
     5041 tttattattc cattctctcg cctttttttt gctgaacccg tcattttcac attttcgcat
     5101 tgttagcatt acatgggcat ggggtttatt atttata
//
