LOCUS       NZ_AP026884             2307 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_11, complete
            sequence.
ACCESSION   NZ_AP026884
VERSION     NZ_AP026884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026884.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_11"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            33..710
                     /locus_tag="OO938_RS23535"
     CDS             33..710
                     /locus_tag="OO938_RS23535"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_264884051.1"
                     /translation="MLDYAIAHNLKCANALAILYATGCRPDELHTGVKINYDKQKNEIR
                     FKIIGSKLNRRMKRGIGIREFSVKINNDNERFFKGIIDLINERPVDSFDHNFKIESAKA
                     FSGYITKISKKLWPRKTYHASAYSFRHAKATELKNSDYDKEKIAQIMGHASVRSQQSYG
                     RKNKKSKGGFNDIEDIKTNVKPRGADRLLRFKIASKNQAAAKIADTSTPSSPPPAPVRR
                     FKM"
                     /name="site-specific integrase"
     gene            1216..1962
                     /locus_tag="OO938_RS23540"
                     /old_locus_tag="KAM546c_46420"
     CDS             1216..1962
                     /locus_tag="OO938_RS23540"
                     /old_locus_tag="KAM546c_46420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_264884050.1"
                     /translation="MDLGNILNESLSIDYEKLDLFLEKYDLTPEQKVAVYEFHAKAYKK
                     NKTLVISETKENKFKSISEGVEYVHLFPKNLKILIKKYGLNTNELLVLTEIMESMLSHG
                     NLLINFSQKALCELTGINKSTMCKTFKTLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFM
                     QFRDHFLNSISYKLDDDEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFE
                     KEISKEWKEKFKDEEENFEFGFESEI"
                     /name="replication/maintenance protein RepL"
     Typing          771..955
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="1.6600000000000002e-73"
ORIGIN
        1 taaataaatt aaatgaaaat tttagagagg atattttaga ttatgcgatc gcgcacaatc
       61 taaaatgtgc taacgcactc gctattttat acgccaccgg ttgccgtccg gacgaactcc
      121 acactggggt taaaataaat tacgataaac aaaaaaatga aattagattt aaaattattg
      181 gatcaaaact taatcgaaga atgaaaagag gtattggaat tcgtgagttt tcagtaaaaa
      241 tcaataatga taatgaaaga tttttcaaag gaattattga tctaataaat gaaagacctg
      301 ttgattcatt cgatcataat tttaaaattg aaagtgcaaa ggcattttcc ggatatatta
      361 caaaaataag caaaaagcta tggccccgaa aaacttatca tgcttctgca tattctttca
      421 ggcatgcaaa agcaactgag ttgaaaaatt cagattatga taaagaaaaa atagcccaaa
      481 taatgggcca tgcgtcagtt agatctcagc aaagttacgg tagaaaaaac aaaaaaagca
      541 aaggtgggtt taacgacatt gaagatataa aaactaatgt taaaccccgt ggtgcagata
      601 gattgctaag gttcaaaatt gccagcaaaa accaagcagc ggcaaaaatt gccgatactt
      661 ccacccccag cagtcctcca ccggctcccg ttcgtcgctt taaaatgtga accgtgagca
      721 gttcaggagg tcccctcctg gactgtgaag ggttggcctg gcggcgagcc ggttttctag
      781 caaaatcctc catattgcga agcagggccc ggaaccgggt aactggatgg ttttcccctg
      841 tgggggattg atctgttact tgaaaaccaa tgatcttaaa aaccatctca aaagttgaaa
      901 atttcacccc cttagtgttc ttaaaattct tagatgttct taggggttta aaaactactc
      961 tccaaccatt gatctaactg actttttaaa aaaggcagtt gtcaaaaact tcaaccgtag
     1021 ttgtcaaatt cgtcaactcc agttgtcaaa ttcgtcaact gaggttgtca aatccgacaa
     1081 ctctagccag tttccaagta gaaactacag tttcaaaact gcaacttttt ctactttttg
     1141 caacttaatc tattgactag ttttatataa atgttaaaac atatatatag aaataaataa
     1201 aaaaaggagg tttctatgga tttaggaaat atattaaatg agagtttaag tattgattac
     1261 gaaaaattag atttgttttt ggaaaaatat gatttaacac cagaacaaaa agttgcagtt
     1321 tatgaatttc acgcaaaagc ttataaaaaa aataaaactt tagttatttc tgaaacaaaa
     1381 gaaaataaat ttaaatctat ttccgaaggt gttgaatacg tgcatttatt cccaaaaaat
     1441 ttaaaaattt taattaaaaa atatggttta aatacaaacg aattattggt tttaacggaa
     1501 ataatggagt caatgctttc acacggaaat ttattaatta atttttcgca aaaggcactt
     1561 tgcgaattaa caggaattaa taaatctaca atgtgtaaaa catttaaaac cctcaaacaa
     1621 aagcagtgtt taattgagaa aaacggacat atttatttaa attctgtgat atttatgaaa
     1681 gggttacctc ataaattgtt tatgcaattt agagatcatt ttttaaattc tatctcatat
     1741 aaattagatg atgatgaaga atttgaaaaa gtcttcgacg ataattttat taaagcatac
     1801 gaaaaaaatc tcaaagagat taaaaagaaa aagcaacaaa taaaagaaaa gaaaatatca
     1861 aaagcattag ataattttga aaaagaaatc tcgaaagaat ggaaagaaaa gtttaaagac
     1921 gaagaggaaa atttcgaatt tggttttgaa tcggaaatat aaaaccgccc tcgccgggca
     1981 ggccaatccc ttactgaaat agaataaatt ctattccact aagggatttt tttattcatt
     2041 gtttctccac atttgcaata ttgacattaa cttccacccg gatataacag tagtataagt
     2101 tgttgtttca acccgtcttt ttgggtggaa caacaaggca ttttatgggt aaagcgaagc
     2161 gaacccgtaa aactgccacc cccaaccggg ggtcgttgtt cgattttgag cgacagcgaa
     2221 aaaatagaac ataaggggga gggtttgggt tttacggtat tttaaatatg agcaaagcga
     2281 atttttaaaa tgtccggttc tttaaat
//
