LOCUS       NZ_AP026883             2816 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_10, complete
            sequence.
ACCESSION   NZ_AP026883
VERSION     NZ_AP026883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026883.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_10"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(2752..2816,1..>1))
                     /locus_tag="OO938_RS23510"
                     /pseudo=""
     CDS             complement(join(2752..2816,1..>1))
                     /locus_tag="OO938_RS23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            <1..345
                     /locus_tag="OO938_RS23515"
                     /old_locus_tag="KAM546c_46380"
                     /pseudo=""
     CDS             <1..345
                     /locus_tag="OO938_RS23515"
                     /old_locus_tag="KAM546c_46380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            397..552
                     /locus_tag="OO938_RS23520"
                     /old_locus_tag="KAM546c_46390"
     CDS             397..552
                     /locus_tag="OO938_RS23520"
                     /old_locus_tag="KAM546c_46390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_053287134.1"
                     /translation="MKKLLFLGGVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            1293..1484
                     /locus_tag="OO938_RS23525"
                     /old_locus_tag="KAM546c_46400"
     CDS             1293..1484
                     /locus_tag="OO938_RS23525"
                     /old_locus_tag="KAM546c_46400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_165459101.1"
                     /translation="MNKQQKTAVNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1484..1906
                     /locus_tag="OO938_RS23530"
                     /old_locus_tag="KAM546c_46410"
     CDS             1484..1906
                     /locus_tag="OO938_RS23530"
                     /old_locus_tag="KAM546c_46410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_021314599.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     TSELIRVFGSLDGVIDAVKHDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(767..1126)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.923"
                     /coverage="84.0"
                     /evalue="1.38e-152"
ORIGIN
        1 tcacgccagc gctggttgtt ttacgcgtat gacaggatac ggaggacggt tgtggcgcac
       61 gtattcggtg aacgcacgct ggccacgctg gagcgtcttc cgggcctgct gtcgaccttt
      121 gaggtcgtgg tatggatgac ggatggctgg ccgctttatg aatcacgcct gaagggagaa
      181 ctgcacgtta tcagcaagcg atatacgcag cgcattgagc ggcataacct gaatctgagg
      241 caaaatctgg caaggctggg acggaagtca ctgtcgttct caaaatcggt ggagctgcat
      301 gacaaagtca tcgggcatta tctgaacata aaacactatc agtaagttgg cagcattacc
      361 ccatttgcct agaataataa aaataaaagg tgatatatga aaaaattact tttcctgggg
      421 ggtgttaccg tttctgtggt tctggcggca tgtcaggtca ataatgttcg tgacaccgga
      481 ggtggggctg tttcaccttc atcgacggtg accggagtca cgcttgatag cggtacgaac
      541 ggcaatccgt aaaggatctt cttgagatcc catttggatc gtcgtaatct cttgctctgt
      601 aaacgaaaaa accgccttgg cgggcggttt tttcgaaggt tcgaggagtt ggcgctcttt
      661 gaaccgaggt aactggcttt cgcagagcac agaaaccaaa ttagttatta cagtgtagct
      721 gtacttcacc tcacacttca acacttcttt gttctgcgaa tccgttacca gtggctgctg
      781 ccagtggcga taagtcgtgt ctttccggat tggactcaag acgatagtta ccggataagg
      841 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
      901 tcccggaacc gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaattaca
      961 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc
     1021 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga
     1081 tgcttgtcag gggggcggag cctatggaaa aacgccccgc cggggcgtgg ccttttcgcc
     1141 cttgtcctta gctttcgtta tgcttaccgt tacgttatct gcttatcttc cctccgctcg
     1201 ccgcagactc atgaccgaac ggagtgagcg aatgagcgag gaagcggaaa actatcagaa
     1261 cgtacagtaa gcactttcgc gccggggcct tcatgaacaa acaacaaaag acagccgtta
     1321 acatggctaa attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg
     1381 atctcgatgc tgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttcc
     1441 gtacactgtc ctcgagactg gatgcattac aggatggtaa ctgatggcca gagtgtctat
     1501 tagtgaagca gcacggcttg ttaaggttag tcgccctact atttacaaga tgattaacag
     1561 tggtaagtta agttacacct ctgttgtaaa gcacgggaag gatattaaag ttattgatac
     1621 atcagagctt attagagtgt ttggttctct tgatggtgta atcgatgctg taaaacatga
     1681 tgtaaaaagt gacgcagaat ctacaggtat taacagcgtt ggtttacatg atttacaaca
     1741 tagaatcgct ttacttgaag ctgaaaacga tggtcttaaa ggggctgtta aggctcgaga
     1801 tgagcatatt gattcccttc gtcaggctat gcagcttctg gagcacaagc acgaaccttc
     1861 ttcaccaccc cattccccgt ggtggaaatt ctggaaaaaa tcctgatccc gccatgcttg
     1921 agttttcctc tccggccatg ccggggcgtt cgtgaacctt cccaaaaatc gccagatctc
     1981 tgaacgctcc tcacactaaa aatatccaca ccccacgcaa aattaaattt ttgcagataa
     2041 ctcattaata tcagtcagtt ggttgttatt aaattacgct gtattacctt ctgtaaggtc
     2101 aatccaagtc gtcggtgatt cgtgtacgac ttgccgacga ctcgtcatag agtcatcgac
     2161 gactcactcc acactatcag taacttatga cgacttgact ctagagtcga atcgtcgttt
     2221 tggcgttttt atgaccgtgg tgactctaga gtcaagtcac gagtcgaatc gccggtgaat
     2281 cgtgtggtga gtcgtccggc gacttgccat aacgtgattg ccggccgtcg acgaacccga
     2341 ttcctgaacg tgctgaaaac cggttttccg gctgcgccgg caacgcttca cggccacgca
     2401 ataaattgcg tccccgttca ccgttttcgg atgggaagaa cgccggaaac aggacttacc
     2461 ctggctgaat agcgtgaatt tccgcactat gcacttgcgc gcatactcat gcatgccgta
     2521 aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg ttttaccggt
     2581 ttcccgtcag aaacgccctg agagcctctc aggcggtgca cgcaacggat gttatggtaa
     2641 atgacaacaa ccgaaatact gtcgtgaatg tgatttttcg attgaaaatc acaaaaaaaa
     2701 tcaacaaata tcacctacta tatcattcga agttagggta atgctgccaa cttactgata
     2761 gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccaccg attttg
//
