LOCUS       NZ_AP026882             2867 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_9, complete
            sequence.
ACCESSION   NZ_AP026882
VERSION     NZ_AP026882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026882.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_9"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(2684..2867,1..383))
                     /locus_tag="OO938_RS23495"
     CDS             complement(join(2684..2867,1..383))
                     /locus_tag="OO938_RS23495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884049.1"
                     /translation="MTKKNNVEILHKEKTNIENDSEIEHKKNPFVFNMSIDTKKKKLTV
                     DRGSEVETEEGVFTTTISQIQEVDREEFLKIFTGQIKFYFDLSQTGFKLFFIIMGLYQK
                     QIGGDEIYLNFETAKEEAEKYESAGLKELLEKKMIAKSVKRYIFYINPAIIFNGDRARF
                     VKEIKIKEEEKQQEENLKILRSKYA"
                     /name="hypothetical protein"
     gene            complement(871..1605)
                     /locus_tag="OO938_RS23500"
                     /old_locus_tag="KAM546c_46360"
     CDS             complement(871..1605)
                     /locus_tag="OO938_RS23500"
                     /old_locus_tag="KAM546c_46360"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_086642456.1"
                     /translation="MGNSKRNIKKLNDNFRESILEYAISHNLKCANALVALYATGCRPD
                     EIETGIKVNFDSKNNKLSFKIIGSKLNYQQKRGIRIREVTVKITDKNLQYYKSIIDIFE
                     KNPQAYDLKIKTESAKAFSGYITKISKKLWPRKKYHASAYSFRHAKATELKNSEGFDAE
                     KIAKIMGHASVRSQQSYGRKSRRSAGGFDDITDVETSSKPRGGDRLLRFKIKNKNEAAK
                     KIKAASTPTDTVSPPPVRSLKR"
                     /name="site-specific integrase"
     gene            complement(1598..2302)
                     /locus_tag="OO938_RS23505"
                     /old_locus_tag="KAM546c_46370"
     CDS             complement(1598..2302)
                     /locus_tag="OO938_RS23505"
                     /old_locus_tag="KAM546c_46370"
                     /inference="COORDINATES: protein motif:HMM:NF013122.3"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]; GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="WP_086642457.1"
                     /translation="MATKIENDEVIPDEELNKIIEEINNEEDKRLKVIVDEYQQLTQNG
                     FFVLDTETTDLVGEVVSFSLIDDKNNILINEYCKPTRNKISKTAQSIHNISNKMIKDSR
                     NIIPIYEDFLKIINTEENKNKTIFIYNEGFDLSTIYRSLNYAGYKYKENNDFDDVEFRS
                     RCVMKDFAYFYGEHNEYYDTDKYKWKKLEFAANYFNIETKNLKLHNSAVDCYLTLKVIE
                     NIRDHFSGRNYG"
                     /name="3'-5' exonuclease"
ORIGIN
        1 gctgactcgt atttttcagc ttcctctttt gctgtttcga aatttaaata aatttcgtca
       61 ccgcctatct gtttttgata caaacccata attatgaaaa acaatttaaa tccggtttga
      121 gacaaatcaa aatagaattt gatttgtcct gtaaaaattt ttaaaaattc ttccctatca
      181 acttcttgaa tttgtgatat tgttgttgtg aaaaccccct cttctgtttc tacttctgaa
      241 ccccgatcaa cagttagttt tttcttttta gtatcaatag acatattgaa aacaaacggg
      301 ttctttttat gttcgatctc actatcgttt tcaatattag ttttttcttt gtgtaaaatt
      361 tctacattgt ttttctttgt catatacaaa ccctctctgt ttaaaaaata accgaaaaaa
      421 ttctcactcg gtgagactct tcttctcatt ttttaacaga tttttgagac tagtcaagaa
      481 aacacattgg atttattctc attttttaca aaaaaaatga gacaaaaagt cacaatatcg
      541 agacatttag tcactgtaga gagaattttt tctctcctga gagaattttt ttctctatag
      601 agactagaca tccagtgttt atgcgggtta cgagacatat aagaatataa gaaatataag
      661 atctaagagg ctttgcttag ctctgagtaa aatttttatc ggcattgtga taactgggct
      721 tcgcaataga aaaccaaaaa ccatctggaa aaccatccca gttaccccga ttccggggcc
      781 ctgcttcgca atatggagga ttttgctata aaaaccgtcc tggccggacg ggccaaccct
      841 tcaaagccac acagcaagct gtgtagcttc tcagcgtttt agagaacgaa ctggaggtgg
      901 acttactgta tccgtagggg tagaagctgc tttaattttc tttgctgctt catttttgtt
      961 cttaatctta aatctcagca atctatcgcc cccacggggc ttagagcttg tttcaacatc
     1021 tgtaatgtcg tcaaatccac ctgcgcttct tctgctcttt ctaccgtagc tttgttgtga
     1081 tctgacagaa gcatgaccca taattttagc gattttttca gcatcaaaac cctctgaatt
     1141 tttcaattct gttgcttttg catgtctaaa agaatacgcc gaggcgtgat attttttcct
     1201 gggccatagt tttttgctta tttttgtaat gtatccggaa aacgcttttg cactttcagt
     1261 tttaattttt aaatcatatg cctgagggtt tttctcaaaa atatctatta tagatttgta
     1321 atattgaaga tttttatctg tgatttttac cgtaacctct ctaattctaa tacctctttt
     1381 ttgttgataa tttaattttg agccgatgat tttaaaactt aacttattgt ttttactatc
     1441 aaaattaact ttaatgccag tttcgatctc gtccggtctg caacctgtgg catataatgc
     1501 cacaagtgcg ttagcgcatt ttagattatg cgaaatcgca tattctaaaa tactttctct
     1561 aaaattatca tttagttttt ttatatttcg ttttgaatta cccataattt ctacctgaaa
     1621 aatgatctct aatgttttca attaccttta aggttaaata acaatcgacg gctgaattat
     1681 gcaattttaa gtttttcgtt tcgatattaa aataatttgc agcaaattcg agctttttcc
     1741 atttgtattt atctgtatca tagtactcat tatgttcacc ataaaaatac gcaaaatctt
     1801 tcattacgca acgagatcta aattctacat cgtcgaaatc gttattttct ttatatttat
     1861 atcctgcata atttaaagat ctatatattg ttgaaagatc aaaaccttca ttataaataa
     1921 aaatagtttt atttttattt tcttctgtgt ttattatttt caaaaaatcc tcatatatag
     1981 gaattatatt tctactatct ttgatcattt tattagaaat attatgtatt gactgtgctg
     2041 tttttgaaat cttattcctg gttggtttgc aatattcgtt aattaatata ttgtttttgt
     2101 cgtcaatcaa tgaaaaacta acaacctcac caacaagatc tgttgtttct gtatcaagta
     2161 cgaaaaaacc gttttgcgta agttgttgat attcatcaac aattactttc aatcttttat
     2221 cttcttcatt atttatctct tctattattt tatttaattc ctcatctgga attacttcgt
     2281 cattttcaat ttttgttgcc ataatttcac ctcatttatt gttttttata ttgttaacat
     2341 caaattccgg agatttcaaa atttcgcttt gctcaaattt gaaataccgt aaaacccaaa
     2401 ccatcccccc attatttaaa tttttcgcgt tgcttcaaaa ttcaaacaat gacccccggt
     2461 tgggggtggc ataatttcgg gtaataaaaa acttaccctt cataatgcct tgttgttcca
     2521 ccccaagaac ggggtggaaa caacaaaaga taatatagtt atatccgggt tgaaattaat
     2581 gtcaatattt gaaatgtgga gaaaatgaga ataaaaaaat cccttagaaa atagaatttt
     2641 tttctatttt ttaagggatt tggcccgtcc ggcgaggacg gttttaagca tattttgagc
     2701 gaagaatttt taaattttct tcttgttgtt tttcttcttc tttaatttta atttctttaa
     2761 caaatcttgc acgatcgcca ttgaaaatga tcgccgggtt aatataaaaa atgtatcttt
     2821 ttacagattt tgcgatcatt ttcttttcta ataattcttt taaacct
//
