LOCUS       NZ_AP026881             3059 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_8, complete
            sequence.
ACCESSION   NZ_AP026881
VERSION     NZ_AP026881.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026881.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_8"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(2446..3059,1..286)
                     /locus_tag="OO938_RS23480"
     CDS             join(2446..3059,1..286)
                     /locus_tag="OO938_RS23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_264884048.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTHEAAGRLDDSQRQDIKDS
                     RTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQYGREMAARVTKQG
                     NVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            289..819
                     /locus_tag="OO938_RS23485"
                     /old_locus_tag="KAM546c_46340"
     CDS             289..819
                     /locus_tag="OO938_RS23485"
                     /old_locus_tag="KAM546c_46340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(1893..2279)
                     /locus_tag="OO938_RS23490"
                     /old_locus_tag="KAM546c_46350"
     CDS             complement(1893..2279)
                     /locus_tag="OO938_RS23490"
                     /old_locus_tag="KAM546c_46350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     Typing          3..283
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="2.14e-52"
     Typing          2447..3054
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="95.0"
                     /evalue="5.53e-132"
ORIGIN
        1 tgaagcagcc ggacgactgg acgacagcca gaggcaggat attaaagaca gccggacgtt
       61 ttcagtcgtg gatgtgcctg aacaggcgaa acatatcgac cacagcgacc gtagctggat
      121 gaatgccagc ggtatcgagg taaaccggaa ggaactggtc agatctctga cgcatgatat
      181 ctcgcagcag tacgggcggg agatggctgc acgggtcaca aaacagggaa atgttgaaat
      241 agagcgcgtg aaatccctgt cacgcggatt ttccataagc aggtaactat ggcgagcacc
      301 gaagaaaaac ttctcacact cctgctgtcg gcggagcatc tgaaagaggc cgcaaaagag
      361 cagcagaaag ccgtggcgga ttcgctggcg gtggcaaggg aaaccctgaa aggctaccgg
      421 gaaatatcgg acagtgtggc acagcaggta agggatgaag tccggcagga agtgcagaaa
      481 atggatgttg cggggctgat tggcgagcgt atcagcgctg attttcagcg tctggaaaaa
      541 tccgtcggga acatgaaaat caatgcggac gcgctggaca aggaaatttc cattatccgc
      601 agtaacatca tcgcggagta caacacgctg cggggttaca gctggaagtg gtttgctggg
      661 gttgcggttg cctttgtcgt actgatattt gccacaagct ggtttgtgaa aacggaacta
      721 caggacaatt acaacgcgtc tgctgccgtc tatcagaaag tggcagacct tgaaaagctg
      781 ctgacaacgg aacagaaacc ggaaaagaaa aagaactgac gcgctgcgct tgtccaacgc
      841 ctcacgttcg cacaataaat tgcgcacccg ttcaccgttt tcagccgagt ttggggcgtc
      901 ttttgagact tccggcctga attcaggccg gaagttctcg ccagtcagtc aggtgcatgg
      961 ttatgcatgg ggctgaaaat cacggtatgc gattctgagc ggttttcggt gagaagccgg
     1021 gggtttttat ggctcaggac gtaaaaccgc cctgagcgcg tttcagcggt gcgcgtaacg
     1081 gatgttatgg taaatccggt ggctttgacg tgcctgccgt tgtgtaccgt cgtgagtgaa
     1141 aataattata ttgttcgaag aacagcgctt tttggccgga gaaaagcagt tctgcaaacg
     1201 atggtatatc gatatgagtt cttttggcat taatccaggc aagacgccgg gcaataagcg
     1261 taatattccg gatgttactt atttccattt ctgtcaaaat gcatcccccc cacgccggag
     1321 aataccatat tagggcaaag aaagcctaat gcccccgtgg tgggtacacc agaaaacggg
     1381 gagcgaagcg accagcatca ggccgttcgt atgctgcttc gattttgtgg gtgttgcgcc
     1441 agaccttggc aatcacgctt caccgcaacc gctgccttca ctctcacttt atcaggtacg
     1501 ttattggtta acgtgagacc gggtagagcc agcttctttc ccgagctggt caggttccca
     1561 ccgcttgcct gtttatcctg ccggagtccg gggcgtagac ctgagagtca aggtgcagat
     1621 atgggtttaa tgtcgttgaa tggtattaag ctggtagtga cattcctagc cagccctgct
     1681 aggatttgat tgtgacgtct gatcctggca ggtatctgca agaattagag tactcaagcc
     1741 ccgtgattct gtcacggggc ttttgtacat ctggagcaag aaaattcatg ctcctatccg
     1801 aaaatcccta ccgctcgccg cagccgaacg accgagcgac gcgagtcagt gagcgaggaa
     1861 gcggcatttc tccggccttg cagaatgcac gttcacgccg atgtttccag atcgtagcgt
     1921 acccgtttca gcgcagtcag taatttttcg tggtcaaaat tcaactcacc cgtttttgta
     1981 tcaaccagtc ccgcgtcaat catcagacca gccgccagaa acagtctgtg attgcgttcc
     2041 ctgcggtttt tctgccctga aatccgcgcc tgaaccgcct ttttctgtgc ggcatcccgt
     2101 tcctgcaacg cgtttttttc agcggttgcc agaacgtgga gcagtcgtct ttcagtctca
     2161 ctgagcgaag cttcatcatc cagcagatcg ataatctgcc gttgccgtgc cgttttttct
     2221 cccagctttc tgatgaaggc tatgcgcttt ttaagccatt cgacgttact gatccgcatc
     2281 gtttaacttc cccatcccga tatttccgac ggggaaagta tataacttgc ggtcagaagt
     2341 cgcatgactt atctttactc ataattcttt aaattagagc aaagcgcact tatatgtttc
     2401 tccctgcggg cagaaacagt gcgccctgcc gggggctgac acaacatggc aacgtatcat
     2461 ctcagcgtga aatttggcgg caagggtaaa gcccttgctc atgccagtta cattacgcgg
     2521 gaagataagt tcagccagcg ccaggatctc gagcacaccg aacacggcaa tatgccggaa
     2581 tgggcgagag atgaacccgc ccatttctgg caggctgcgg atgcgttcga acgtgccaac
     2641 ggttcaacgt acagggaaat tgagatcgct ctaccccgcg agctgaatga agcgcagcgg
     2701 ctggcgcttg tccgggattt tgtgaagcag gaaacgggcg acaaacatgc atggacgttt
     2761 gcgatccaca atccgaaagc cagcattgac ggcggcgaac agccgcacgc ccatatcatg
     2821 atgagccagc gcgtcaatga cggtattgag cgcagtcccg aacagtattt caggcgctat
     2881 aacgccaaat ttccggcgcg tggcggcgcg cgcaaggact cagggcataa tttaacccct
     2941 acagaacaaa aacaggcact gaaagccctg cgggagcgct gggagcgtac gcacaacgcg
     3001 catatgaaac gatatggctt tgagcagggt tttattgata gccggtcact gaaaacaca
//
