LOCUS       NZ_AP026880             3953 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_7, complete
            sequence.
ACCESSION   NZ_AP026880
VERSION     NZ_AP026880.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026880.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_7"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(3359..3953,1..176))
                     /locus_tag="OO938_RS23450"
     CDS             complement(join(3359..3953,1..176))
                     /locus_tag="OO938_RS23450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_264884045.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLVEKPAR
                     AGKLPSISPALLRHEGASVLQGKPEEVRELIDASPYAKKYTSGVLSFAEQDLPPGQRET
                     LMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLVPNTELLTGKRLQPYYDRADRPR
                     IDAWQTIVNGRMGLHDPNAPENRRALVTPSALPEAKQEAAQAITRGLLALASSGELKTR
                     QDVTEALESAGFEVVRTTKSSISIADPDGGETSD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            805..912
                     /locus_tag="OO938_RS23640"
     CDS             805..912
                     /locus_tag="OO938_RS23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_319965302.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHAKAPLAAAQ"
                     /name="replication initiation protein"
     gene            complement(<959..1534)
                     /locus_tag="OO938_RS23455"
                     /pseudo=""
     CDS             complement(<959..1534)
                     /locus_tag="OO938_RS23455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /name="diguanylate cyclase"
     gene            complement(1531..2004)
                     /locus_tag="OO938_RS23460"
     CDS             complement(1531..2004)
                     /locus_tag="OO938_RS23460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="WP_264884047.1"
                     /translation="MFNSVLVVSNGAVVLATSPESLGLVGVHLNSSASKLAIEGKKPFV
                     SAPYKSATGNLIVLLSHPIYDKNGNYIGYVGGSIYLKKHSLFSDVLSRHFFKSDTEISI
                     VSDDGTVIFNNNNSAVGRPMVMSDDLKSKLTESDRGEGDFFLRGIDIFWVMLI"
                     /name="PDC sensor domain-containing protein"
     gene            complement(2067..2294)
                     /locus_tag="OO938_RS23465"
     CDS             complement(2067..2294)
                     /locus_tag="OO938_RS23465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884043.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIVVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMGNYIEMAQSELAYGAKK"
                     /name="hypothetical protein"
     gene            complement(2535..2762)
                     /locus_tag="OO938_RS23470"
     CDS             complement(2535..2762)
                     /locus_tag="OO938_RS23470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884044.1"
                     /translation="MADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVMRLDGIEA
                     HREQERAAQHQKALELERSQRQRTYNGPSL"
                     /name="hypothetical protein"
     gene            complement(2861..3346)
                     /locus_tag="OO938_RS23475"
                     /old_locus_tag="KAM546c_46330"
     CDS             complement(2861..3346)
                     /locus_tag="OO938_RS23475"
                     /old_locus_tag="KAM546c_46330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_119002157.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYMTIREQKSTQAMLSERNSGVQLTTCGEQRRRCVRVNPEGGTFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(271..443)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="2.01e-77"
     Typing          368..906
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.265"
                     /coverage="89.0"
                     /evalue="0.0"
     Typing          complement(2539..3386)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.873"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgaagca gcgccggcga gatggaggga agtttgccgg cacgggcggg cttttcgacc
       61 aggcacgtct gccgcatcca ggccgcgagt tgcttaccct cgcagcgctc cagcagacgc
      121 cggtgctcat cctcagtcac ccacatcgtg agcattttgc tgcgtttgtc tgccaatccg
      181 gcctcctgat aacaccggga cgggcgggaa attctttccc ggtcggcgcg gtgttggaca
      241 agccgcaggc gcgtcagtgg cttttggcgg gtttcggggc gcagccctga actagtcacg
      301 tagcgctagc ggagcgtata ctggcttact atgttggcac ggatgagggt gtcagtgaag
      361 tgcttcatgt ggcaggagaa aaaggcggca ccggtgcgtc agcagaatat gtgatacagg
      421 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcggct gcggcgagcg
      481 gaaatggctt acgaacgggg cggaaatttc ctggaagatg ctaggaagaa acttaacagg
      541 gaagcgataa ggccacggtg ttgccgtttt tccataggct ccgcccccct gacaagcatc
      601 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
      661 cgtttccccc tgatggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
      721 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggaag
      781 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
      841 ttgtccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc gccactggca
      901 gcagcccaat aacactccta atacgctccg caacacactc tgctgttaac acagatgtat
      961 tcggcagaaa aacaacaaac tcttcgccac caaaccgaca aataatatct tcactacggc
     1021 aacaagactt aataatctga gccaaagaaa caagcacctc atctccgacc ccatgcccga
     1081 aactatcatt tattttttta aaaaaatcaa catcaatagc aataactgaa gctcctgccc
     1141 catacttgtt attctgtatt ttagttgaaa atcccaatct attgttcacg ccggtaaggg
     1201 gatctttaac cgccacctca tttaaagaat caaccctttc catcattacc ttaatatgcg
     1261 taaaaagtgc ctttttaagt cgatcagctt cggcatacca agcattgata gctttaatat
     1321 aaactagcga agctcctata tctttagtat tggtagatat agcaagcttt tctaaaggaa
     1381 ttgaaatacg ggaggcaatg aaatatgaaa gaagtgaaaa tgcaataatt actacaatta
     1441 gcagaacaag tacactttta gcataagtaa taagaatagt agtcgcgctg tcagcatgac
     1501 tataaaccaa cacattccaa gcagtatttt tcatatgagc ataacccaaa agatatctat
     1561 tccccgaaga aaaaagtccc cctcacctct atcagactca gttaactttg atttcaaatc
     1621 atcagacata accatgggcc gaccaacagc actgttattg ttattaaaga tcacagttcc
     1681 atcatcacta acaatgctaa tttcagtgtc gctcttgaaa aaatgcctgc tcaaaacatc
     1741 gctaaacaaa ctatgtttct ttagatatat agacccgccg acatacccta tgtaattgcc
     1801 attcttatca taaataggat gtgacaacaa aacaatcaaa ttcccagtag ctgatttgta
     1861 aggtgccgat acgaatggtt ttttgccctc aatcgccaat ttgctggcac ttgaattcaa
     1921 gtggacacca actaaaccaa gtgattctgg cgaagtcgcc agaacaacag ctccattact
     1981 aacaacaaga actgaattaa acataccaga ctgcaatctt aacctatccg cctcattttt
     2041 aagtacatca atgtcattag ttgattctat ttttttgcac catatgccaa ttcactttga
     2101 gccatttcaa tataattacc cattacatct gacattttca tagcgtacga tgaattagca
     2161 tcaagcaagt ctttttctat attttcgcgc tgaaaaacca taataacgac aatcagaaaa
     2221 atagaagtca acaaaatccc accaacggat aaggttatga ctatcggacg aaggtgaggt
     2281 tttttaattc tcataaaaat acactccata taattaaaaa atgccgcatt taccataaca
     2341 tcccttaccg gcaccgcgcc gggcaggttc ggcgcgaata gggtgcatgc cgggtaaaac
     2401 agacagtaaa ccatcccgaa aaccgcgcat tctgcacctg ataaatttca ccggattgca
     2461 tagccatgca gcagggtgaa tggcaccggc tttcggcgca gatacccgcc ggaagcctcg
     2521 ttttatccga attgttacag cgacggtcca ttgtatgtcc gctgccgctg cgatcgttca
     2581 agctccagcg ctttctgatg ctgcgccgcg cgctcctgct cccggtgtgc ttcaatgccg
     2641 tccagtctca tgacaaccgg cgcagctgct cgttcaaact ctgcacctgc ctgctcaagc
     2701 ccgtgacgcg ctcgctcagc gccgcgttct cccgttgcat aagaccagac atcgtccgcc
     2761 attccgcgaa ggcgttctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct
     2821 ctaatgcgct cagcaactgt ttttccagct ccgtcatgtg ctatttcccc gccagtatca
     2881 tccagctcga atcctctccg aacgtccccc cttccgggtt caccctcacg cagcggcgtc
     2941 tctgctctcc gcaggtcgtg agctgcacgc cgctgttcct ctcggacagc atggcctgcg
     3001 tgctcttctg ctcccggatg gtcatgtaat tgtcgagtat ctgctgcccc tgccaccaca
     3061 gaatgcccgc gctcgaggcg atcagcagcg cggagaccag cacgatggtc agccacgtct
     3121 ggctgaccat gcgcagcatc cctttcgttc gctggctcat ggctgacgac agcttccggt
     3181 cgtggtcgag gatggcggcg ctgattctct tctcgctttc gctcagttcc gctctgacag
     3241 acttctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtagtctgc tgctgcgctt
     3301 tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctcccttc
     3361 agtcggatgt ttcgcccccg tccgggtcgg caatgctgat gctgcttttt gtggtgcgca
     3421 ccacttcaaa acccgcgctt tccagcgcct cagtgacgtc ctgacgcgtt ttcagctccc
     3481 cggacgaggc taaggccagt aaaccccgcg tgatcgcctg agctgcctcc tgcttcgctt
     3541 ccggcagcgc ggaaggcgtg accagcgccc gccggttttc cggcgcgttc gggtcgtgca
     3601 gccccatcct gccgttcacg atggtctgcc aggcatcgat gcgcggacgg tcagcgcggt
     3661 cgtaatacgg ctggagccgt ttgccggtca gcagctccgt gttcggtacc agaaaattaa
     3721 gctccagacg gcccttgtcc tggtgttcaa cccacagcac gctgtactgg tctttatcga
     3781 gtccgggcat cagaacccgc tcgaaactcg ccatcagcgt ttcacgctgg ccgggcggta
     3841 agtcctgttc ggcaaaggac aggacgccgg aggtgtactt ttttgcataa ggcgaggcgt
     3901 cgataagctc ccggacttcc tccggcttcc cctgcagaac gctggcacct tcg
//
