LOCUS       NZ_AP026879             4053 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_6, complete
            sequence.
ACCESSION   NZ_AP026879
VERSION     NZ_AP026879.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026879.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_6"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(262..411)
                     /locus_tag="OO938_RS23430"
                     /old_locus_tag="KAM546c_46280"
     CDS             complement(262..411)
                     /locus_tag="OO938_RS23430"
                     /old_locus_tag="KAM546c_46280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            982..1140
                     /locus_tag="OO938_RS23635"
     CDS             982..1140
                     /locus_tag="OO938_RS23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1497..2096
                     /locus_tag="OO938_RS23435"
                     /old_locus_tag="KAM546c_46290"
     CDS             1497..2096
                     /locus_tag="OO938_RS23435"
                     /old_locus_tag="KAM546c_46290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049002646.1"
                     /translation="MKLINSFALSCFFFSISALAAQELVINNSALHIIFADGTEANVSS
                     CSDFISLNQAGMKIKELPDLSDPDYQQAHMELSKCLMHSWAIQNGYTQKTSSAITLDEV
                     VKHFPATADLSPASGYKTMLQTIPDLKPKDDRFVSHTKEEGYVINDAWTFKNSSGQEAD
                     FVMLVGYATGGTMAVARLWRIDSKTTTPWGVTEIKP"
                     /name="hypothetical protein"
     gene            complement(2123..2905)
                     /locus_tag="OO938_RS23440"
                     /old_locus_tag="KAM546c_46300"
     CDS             complement(2123..2905)
                     /locus_tag="OO938_RS23440"
                     /old_locus_tag="KAM546c_46300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884042.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVT
                     VGIGRMLPNSQAGYNLGFTKTHHIPRGHGDSEDVEISASHGDIDSAFNATKEKRASNVH
                     LSDQSMVADCVNQLDKDRTTLRNMYTDFDHFPQPAKTALHDMLYNLGEGKLHSEFPNFN
                     AAVNRKDWSRAATESHRTGIGDSRNKDTHDQFIAAGNK"
                     /name="hypothetical protein"
     gene            3123..3563
                     /locus_tag="OO938_RS23445"
                     /old_locus_tag="KAM546c_46310"
     CDS             3123..3563
                     /locus_tag="OO938_RS23445"
                     /old_locus_tag="KAM546c_46310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_103142902.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQVENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     Typing          complement(447..620)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          544..1149
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc cgccatggtt
       61 gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt tcatcgcaac
      121 tgcatgaatt aaaagaaaaa aaaggaatat ttcgttttca cgagtaatac ccgggtatta
      181 aaaatccact gtaattacat gaaaataaag aaataaaagg taataccggc gtattaccac
      241 gtacgcgggc aagcgaaaaa gctataacgc ttcccggccg acaggcgacc caaaatccac
      301 aggggcatgc aggttctctt cagtaacctg cgccattaaa tcaaaaaggt catattcgcc
      361 gggaagctgc gtatcagtcg aatccagttg ttcatcatat tcagatggca tggggtgata
      421 tccagtagct gagaggttgt tagcgggttt cggggcgcag ccctgaacca gtcatgtagc
      481 gctagcggag tgtatactgg cttactatgt tgccacgatt gcgaaaatca gtgaagtgct
      541 tcatgtggca ggagaaaaaa ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga
      601 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta
      661 ccggcttacg gacggggacg gaaatttcct ggaagatacc aggaagacac ttagcaaaga
      721 agagataagg ccacggcgaa gccgtttttc cataggctcc gcccccctga caagcatcgc
      781 aaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg
      841 tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
      901 attccgctgt tatggccgcg gtttctcatt ccacgcctga cactcagttc cgtgaaggca
      961 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg
     1021 tccggtaact atcaacttga gtccaacccg gaaagacacg acaaaacgcc actggcagca
     1081 gccattagta acaggagtgc acgagaagat agattaagag gtcttgaagt agaggcctga
     1141 ctgcggctac actgaaagaa cagttttggt aatggcggtc tcgtgaaccc tgttaccggg
     1201 ttaagcagtt ccccaactga cttaaccttc gaacaaaccg cctccccagg cggttttttc
     1261 gtttacagag caagagatta cgattcattg aaaaggatct caagaagatc ctttgtagat
     1321 tactatcaat taatcttccg aacatgacac cagtcactgt tgattacggt taaaccgctc
     1381 cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa agaagcaaaa aagataaata
     1441 tatctactta ttgagagatg atatatttat ttccaaaatg ctacagatac atcatcatga
     1501 aactaattaa ctcattcgcc ctgtcatgtt tcttcttttc aattagcgcc ctcgccgcac
     1561 aagagctggt aattaacaat tcagccctac acattatctt tgctgacggc acagaggcta
     1621 atgtatcctc atgcagtgac tttatctctc taaatcaagc aggtatgaag attaaggagc
     1681 taccggatct gtcagaccct gattaccagc aggcgcatat ggagctcagc aaatgcctaa
     1741 tgcattcttg ggctatccag aacggatata cccaaaaaac cagttcggca atcacacttg
     1801 atgaggtagt gaagcacttc cctgcgacag cagacctgag cccagcctct ggttataaaa
     1861 ctatgctgca aacaataccg gacttaaaac cgaaggatga ccgctttgtg tcacatacca
     1921 aagaggaagg gtatgtgatt aatgatgctt ggacatttaa gaactcatcc ggccaagagg
     1981 cagatttcgt gatgttggtg ggctatgcaa ccggaggtac aatggctgtt gcccgtctct
     2041 ggcgaataga ttcaaaaacc accaccccct ggggtgttac cgaaataaag ccataaaaaa
     2101 agccgcatta tgcggctttt tcttatttat tacctgcagc aataaactgg tcgtgtgtgt
     2161 ctttgttacg ggaatcaccg atgccagtgc gatggctttc agtcgcagct ctgctccagt
     2221 ctttacgatt tactgccgca ttgaaattag ggaattcaga gtgcaatttc ccttctccca
     2281 aattatacag catatcatgc aatgcagtct tggctggttg cgggaagtgg tcgaagtcag
     2341 tatacatgtt gcgcagggtg gtacgatcct tgtccagttg attcacgcaa tccgcaacca
     2401 ttgactgatc tgagagatgg acattggatg cccttttctc tttggttgcg ttaaacgcgc
     2461 tatctatatc accatggctt gcgcttattt ctacatcttc tgaatctcca tgaccacgcg
     2521 gaatatggtg tgtcttagta aatcccagat tatagcctgc ttgcgaattc gggagcatgc
     2581 gaccaatccc cactgttaca ttgttcgcag tatctaggta catgttattg cgtgcacctt
     2641 catgatgtat aatgttatta atgacagcag aaagatcgac agaattaata ttcttcgtac
     2701 ctgggacagt tatcgggtgt cggccagtga tgagaccaaa ggatgcagta taagatgaac
     2761 cactactatt atcatggcca ccattaccac caccgttaaa actacctccc gaacttccag
     2821 agccgctatg accatcattg taggggtcat ttggtcccat gtgaccaaaa ccaccgacag
     2881 taccgctatt taactctgta ctcataaaat tatcctcttt gacttttaaa aacagcaaaa
     2941 actgtattta taacaatgtg tttatataca gtattcgaaa tacttcgccc gtcaaaacca
     3001 caccgtcaac aggttttttg tgtatttttc acgcctcacg ggcagcctca gaccttttgt
     3061 gttaaaacag taatatgttc tgctgttaca atacggtaca cacaggagac actgtcactt
     3121 ttatggcatg gcacacagtt caagagacac tgaaattaac aggaaagtcg cgctcgcaat
     3181 tatatcgtga catggctaaa ggccttgtca gctatcgcac aggaaaaaat gatcgccggg
     3241 agttcgagac cagtgaactg attcgggcct atggcgagct gcgcacttct gagacaccaa
     3301 aaaggcacaa ggagggacac tccaaatctg tcgttagcca ggtggaaaat gctcagctag
     3361 acgtcattca gcagaaactg gacgctctgc agcagactgt taccctaatg cttgaagaca
     3421 agatggcgcg ggaagcagaa aaccgcctgc gaaatgagga gagggaacaa ctacaggccg
     3481 aaattagccg tctggctgac gcgctggcac aggaaaaaaa gcggggattc tggtcgcggc
     3541 tgtttcgctc cggttcagag taacagtgca ggtgactctg atttaccata acatctgtta
     3601 tcagcaccgc ctgagaggcc ctcagggcgt ttctgacggg aaaccggtaa aacaggcaac
     3661 aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat
     3721 gcgcacaagt gcatagcgcg gaaattcaca ccattcagcc ggggtaagtc ctgtttccgg
     3781 cgttcttccc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt
     3841 tgccggcgca gccggaaaac ccgctgtcag cacgttcagg aagggggtga tgtacaaatg
     3901 gcacagagga tgaaagtgac gtatgagctg aaggaatgat gtaaaagtga tgtataagtg
     3961 atgtaaaagt gttgtaaaag tgatgtatga actgaaggag cgatgtaatt ctgtagtcag
     4021 gaagaataac ccgttcatgc aggcatgaac gat
//
