LOCUS       NZ_AP026878             4697 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_5, complete
            sequence.
ACCESSION   NZ_AP026878
VERSION     NZ_AP026878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026878.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_5"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(4389..4697,1..12)
                     /locus_tag="OO938_RS23400"
     CDS             join(4389..4697,1..12)
                     /locus_tag="OO938_RS23400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_264884041.1"
                     /translation="MANSFNVTIAEAALWSLQDAESFKSNYTDSVQAALWADDLLASSI
                     KAISQDPDRYRYNAMLADKGISIRERLDPDNQYRCLYDFDEQNGVIEILLFVSTKQDLV
                     VK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(100..531)
                     /locus_tag="OO938_RS23405"
                     /old_locus_tag="KAM546c_46230"
     CDS             complement(100..531)
                     /locus_tag="OO938_RS23405"
                     /old_locus_tag="KAM546c_46230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086073960.1"
                     /translation="MDWYDVKWFFGDVCDSVSEVAGNIYDTAADGVAAVTDFIEENPKT
                     SIAIGALVGIATAGIAAPAIAATIGATGALGTTAGGTVIANLSGAALSSSSLAALGGGS
                     IAAGGAGMAGGTCVVAAGGGVVAGSVSAGGVAATKAIKS"
                     /name="hypothetical protein"
     gene            complement(850..1521)
                     /locus_tag="OO938_RS23410"
     CDS             complement(850..1521)
                     /locus_tag="OO938_RS23410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223364945.1"
                     /translation="MLIVILSLYYTITEPFKSELIKNLSYSLIATFIFDTGLNFSKENI
                     TKSIVSRKWHNSLYFAFERLRAIHEIFTAQNKLEITKELASDMASSILMENGAVISKKH
                     IKTMWNPSSGEPLTYQDIVIKEGDNLYFASMMFIRDDYEFLHSIYFDENIFLSFPQLLK
                     PAQRCYRSLAMIQHNINSKQKFNVTKKSLERDLINYLELRSTLLMKMEDVLGHYEQRAP
                     Q"
                     /name="hypothetical protein"
     gene            2453..2644
                     /locus_tag="OO938_RS23415"
                     /old_locus_tag="KAM546c_46250"
     CDS             2453..2644
                     /locus_tag="OO938_RS23415"
                     /old_locus_tag="KAM546c_46250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2644..3066
                     /locus_tag="OO938_RS23420"
                     /old_locus_tag="KAM546c_46260"
     CDS             2644..3066
                     /locus_tag="OO938_RS23420"
                     /old_locus_tag="KAM546c_46260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            4105..4374
                     /locus_tag="OO938_RS23425"
                     /old_locus_tag="KAM546c_46270"
     CDS             4105..4374
                     /locus_tag="OO938_RS23425"
                     /old_locus_tag="KAM546c_46270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024167138.1"
                     /translation="MSITMSQKSAREGLGNPELFQGGVYVTKNGLAELFVQTAEEREAE
                     LRERERERQSNALLKLVMAAKREIKNGRGMSPEDAMRRLREART"
                     /name="hypothetical protein"
     Typing          complement(1925..2332)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.634"
                     /coverage="99.0"
                     /evalue="2.7200000000000002e-179"
ORIGIN
        1 gttgttaaat agatgttaaa tgcaaaagcc cgcttttata gtgggctttt tatatggtag
       61 aaactttaag atgtcgacgt tatggtaaat ggaagcttgt tagcttttta tcgcttttgt
      121 tgctgccact cctccagcag aaacgcttcc agcgaccact cctcctcccg cggctactac
      181 acatgttccg ccagccatac cggctcctcc agctgcgatg gaacctccac ccaacgctgc
      241 taaagacgag ctagataatg ctgctccact taagtttgct attacggtac ctccagctgt
      301 agttcctagc gctccggttg ctccaattgt tgccgctatt gccggagctg ctatgcctgc
      361 tgtcgcaata cctacaaggg ctcctatcgc aatggatgtt tttggatttt cttcaatgaa
      421 atcagttaca gccgctaccc catcagctgc agtatcgtaa atattgcctg ccacctctga
      481 aacagagtca cagacgtctc cgaaaaacca tttaacgtcg taccagtcca tatgctaccc
      541 tcttaaccat atgatttatt aagtaattaa tcttttttga ttgcacggaa gatataatac
      601 acatattaaa agcaatgttt tttaaaggca ataaaatgat cgcaggccca tgcataatta
      661 tggtgagtgg aactggttat gttggtaaat tcttcgatgg ttcgatagat tggattgaaa
      721 gttgctacaa atagctttca ggggggggga acgccaaacg ttttttattt atgcacaatc
      781 atttctttat gttcaatctt acttatctca ttgccttttt taaatttaaa gatgaaagtg
      841 acattgatat tattgtggtg ctctttgttc atagtggcca agaacatctt ccattttcat
      901 taataatgta gatctcagtt ctaaataatt aattagatct ctttctaatg atttttttgt
      961 gacgttaaat ttttgtttgc tgtttatatt atgctgtatc attgctagtg atcggtaaca
     1021 tctttgggct ggttttagta gttgagggaa ggagagaaat atattttcat caaaataaat
     1081 actgtgtagg aattcataat catctctgat gaacatcata gacgcaaagt ataagttatc
     1141 cccttctttt attactatgt cttgatacgt aagtggttct cctgaagaag ggttccacat
     1201 tgtttttatg tgttttttag atataacagc tccattttcc attagtatgg atgacgccat
     1261 gtcgctggct agttcttttg tgatctctag tttgttttgt gctgtgaata tttcatgtat
     1321 tgctcttaat ctttcaaatg caaagtataa agaattgtgc catttcctgg atactatact
     1381 ttttgttata ttttctttgc tgaagtttaa gccggtgtca aaaataaaag ttgcgattaa
     1441 tgaataggat aagtttttaa ttaattctga tttaaatggt tctgtaattg tataatagag
     1501 agataatata acaattaaca ttagtgattg tcgtattgca ccagatgaat atatgtataa
     1561 acgatatcta tttaatggtg cttttatttt ttgaggaatt agagatattg atttatattg
     1621 tctcctcggg ggtgaaacaa aaaatacatg tgtatgtgaa taatataatg aataatataa
     1681 agttaaccaa tcttatcact cctcgatatc ggtgatgaat gattttcttg agatcttcag
     1741 caagtcatcg taatctcttc cctgctaata agaaaaaacc gccttgcagg gcggtttttc
     1801 gaaggttcta agagttggcg ctcgttgaac cgaggtaact ggcttggagg agcgcagtca
     1861 ccaaaacttg tcctttcagt ttagccttaa ccggcgcata acttcaagac taactcctct
     1921 aaatcaatta ccagtggctg ctgccagtgg cgcttttgca tgtctttccg ggttggactc
     1981 aagacgatag ttaccggaca aggcgcagcg gtcggactga acggggggtt cgtgcataca
     2041 gtccagcttg gagcgaactg ccttcccgga actgagtgtc aggcgtggaa tgagacaaac
     2101 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     2161 gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     2221 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggcc
     2281 gctgcgcggc ctttccttct ttcctccatg cgattgctcg gttttctcga gcgtttctac
     2341 gcatgctcct gccgctcgcc gcagccgaac ggcaggagcg tagcgactga gtgagcgagg
     2401 aagcggaaaa gagaacatcc ctacactgag cactgacgct ctgaggcctc ttatgacgaa
     2461 acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc tgctcctgct
     2521 ggagaagctt aacgagcttg atcttgatgt tgaggccgac ctttgtgaga aactccacga
     2581 tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgaattac aggatggtaa
     2641 ctgatggcca gagtgtctat tagtgaagcg gcacggcttg ttaaggttag tcgccccaca
     2701 atttacaaga tgattaacag tgggaagtta agttacacct ctgttgtaaa gcacgggaag
     2761 gctattaaag ttattgatac ttcagagctt attcgagtgt ttggctctct tgatggtgtc
     2821 atcgatactg taaaatatga tgtaaaaagt gacgcagaat ctacaggtgt taacagcgtt
     2881 ggtttacatg atttacaaca tagaatcgct ttactagaag ctgaaaacga tggtcttaaa
     2941 ggggctgtta aggctcgaga tgagcatatt gattcccttc gtcaagctat gcagcttctg
     3001 gagcacaagc acgaaccttc ttcaccaccc cattccccgt ggtggaaatt ctggaaaaaa
     3061 tcctgacccc gtcatgcttc agttttcctc tccggccatt ccggggagtt cctgaacctt
     3121 caaaaaatcg ccagatctct gaacgctcct caccactaaa aatacccaca cactacgcaa
     3181 aattaaaatt ttgcagattc cttatttaaa acaatttgtt aatgaaaaat taatgtgcag
     3241 tgatgtgtta caaagtgcag atccaagtcg tggacgactc tatggcgaat cgtttacgaa
     3301 ttgcccacga ttcatatgtg aatcacgctt tattatcatg ttgttatgat gaatcgactc
     3361 ttgattcaat tcatcaattt gggcgttttt gttcatggtg actcttgatt caattcacga
     3421 ttcgaatcgc cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc
     3481 ggccgtcgac gaacccgatt cctgcacgtg ctgaaaatgg tttttccggc tgtgccggca
     3541 acgcctcacg gccacgcaat aaattgcgca tccgttcacc gttttcggat cagaagaacg
     3601 ccggaaacag gacttaccct ggctgaatgg cgtgaatttc cgcgctatgc acttgcgcgc
     3661 atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt
     3721 gttgcctgtt ttaccggttt cccgtcagaa acgccctgag gccgttttag cggtgcgtac
     3781 aatcatggct ttatgttaaa taacagacga aaaaatctcg atagtagaag aatggggatt
     3841 gttcctgagc tggggatgcc ttactgatgc gcgtaatttt cgtttaatag gaggttggtc
     3901 gaagctccag aatcttatat ttgaatggtg gtgtatatat ttatgataaa tttgtactcc
     3961 cctgtgagtt ttgatacaat ataactgcca ttcaccctaa gtggcagcaa gatgtgaggt
     4021 ctttttgacc tctttttcat tcaggatcta gtcgcttatg cttaatcttg cgtgtaagtg
     4081 gctgttttaa ttaaaggtat aagtatgtct ataactatgt ctcagaagtc agcccgtgaa
     4141 ggtcttggca accctgaact gttccaagga ggcgtttatg ttacgaaaaa tggtttagca
     4201 gagctgttcg tgcagaccgc agaagagaga gaggccgagc tgcgtgagag agaaagagaa
     4261 cggcaatcaa atgctctttt gaagctagtc atggctgcaa agagagagat aaaaaatggt
     4321 cgtggaatgt cccccgaaga tgctatgcgg aggttacgtg aagcgcgaac ttaatataag
     4381 gagccaactt ggcaaattca ttcaatgtaa cgatcgcgga agcggcacta tggtctctgc
     4441 aggatgcaga atcctttaaa tcaaactaca cggatagtgt tcaagctgcg ttatgggctg
     4501 atgatctttt ggcttcatcg ataaaggcca tttctcaaga tcctgatcgt tatcgctaca
     4561 atgctatgtt ggctgataaa ggaatatcga ttcgtgagcg tttggacccc gataaccagt
     4621 atcgttgttt atatgatttt gatgaacaaa atggtgtcat tgagatcctg ctttttgtta
     4681 gcactaagca agatctt
//
