LOCUS       NZ_AP026877             4789 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_4, complete
            sequence.
ACCESSION   NZ_AP026877
VERSION     NZ_AP026877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026877.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_4"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(4616..4789,1..136)
                     /locus_tag="OO938_RS23345"
                     /pseudo=""
     CDS             join(4616..4789,1..136)
                     /locus_tag="OO938_RS23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /name="addiction module toxin RelE"
     gene            138..422
                     /gene="nadS"
                     /locus_tag="OO938_RS23350"
                     /old_locus_tag="KAM546c_46160"
     CDS             138..422
                     /gene="nadS"
                     /locus_tag="OO938_RS23350"
                     /old_locus_tag="KAM546c_46160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_012442910.1"
                     /translation="MSFFDELKASLEEAVEIKKGVKAPARVTRYELADVKALRAQLNVT
                     QDEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELASH"
                     /name="nadS"
     gene            618..1520
                     /locus_tag="OO938_RS23355"
                     /old_locus_tag="KAM546c_46170"
     CDS             618..1520
                     /locus_tag="OO938_RS23355"
                     /old_locus_tag="KAM546c_46170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_264884038.1"
                     /translation="MTNPALDLFNDRLPNKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRIGAAIDWSDRNAPAPTMTITNPENGHAHLLYALKNSIRTAPDGKMK
                     PLKYAAAVENALRKKLDADVGYAGLICKNPNHGHWKIAVWQPELYTLDWLADSLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLEACYERANAYNLQFTSPLD
                     EKEVGGIAKSIAKWTHHQLSPEGFAEYVSRTHAPSIQAVRGRKSRGGGRPKDSSALVNT
                     KPWLSLGISRATFYRKQGG"
                     /name="replication initiation protein"
     gene            complement(1517..1687)
                     /locus_tag="OO938_RS23360"
     CDS             complement(1517..1687)
                     /locus_tag="OO938_RS23360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884039.1"
                     /translation="MFDFIGDYVCSFIIARIVGNLLLQLVMTLCSLPVMNLIVIFAILV
                     TLFAIAREFKN"
                     /name="hypothetical protein"
     gene            2194..2520
                     /gene="mobC"
                     /locus_tag="OO938_RS23365"
                     /old_locus_tag="KAM546c_46180"
     CDS             2194..2520
                     /gene="mobC"
                     /locus_tag="OO938_RS23365"
                     /old_locus_tag="KAM546c_46180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_001715145.1"
                     /translation="MEKRTREIKIRLTDSEHQRLIDRCDRTHLAEWLRQLGLGEHTARK
                     RRLPDVAPELLRQVSGIGNNLNQIARRLNQLDGLNPTERVSLLAVLNSLDRNLGELLEQ
                     HRDR"
                     /name="mobC"
     gene            complement(2528..2800)
                     /locus_tag="OO938_RS23370"
                     /old_locus_tag="KAM546c_46190"
     CDS             complement(2528..2800)
                     /locus_tag="OO938_RS23370"
                     /old_locus_tag="KAM546c_46190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884035.1"
                     /translation="MLNPQASVLVFVQTGHQCLFPFVHKTRLFRRGNVGFLKGQHARGV
                     GAGKVTAVNQFAGAFGVSPQHAGVTALAIFAQQVINGATARTGTP"
                     /name="hypothetical protein"
     gene            2729..>3148
                     /locus_tag="OO938_RS23375"
                     /pseudo=""
     CDS             2729..>3148
                     /locus_tag="OO938_RS23375"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3198..>3293
                     /locus_tag="OO938_RS23380"
                     /pseudo=""
     CDS             3198..>3293
                     /locus_tag="OO938_RS23380"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            3381..3683
                     /locus_tag="OO938_RS23385"
     CDS             3381..3683
                     /locus_tag="OO938_RS23385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="WP_264884040.1"
                     /translation="MIRATATTALIWIVVCAALLIGILSGIIWFQGQIISSRLSDIDGY
                     SQQLASLKAKSGAGVTIYSDDSQKNGYLVVLPVKAHGVETYTSTAGNTVVKYTAK"
                     /name="sialate O-acetylesterase"
     gene            3744..3980
                     /locus_tag="OO938_RS23390"
                     /old_locus_tag="KAM546c_46210"
     CDS             3744..3980
                     /locus_tag="OO938_RS23390"
                     /old_locus_tag="KAM546c_46210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_264884036.1"
                     /translation="MTEMEKQLLSALESLQASYEQQHQAWQESYSSLQHMFETTSQALN
                     RSDRVCQHLSNQVNSLRGQVESLSDNVSRLMRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4084..4521
                     /locus_tag="OO938_RS23395"
                     /old_locus_tag="KAM546c_46220"
     CDS             4084..4521
                     /locus_tag="OO938_RS23395"
                     /old_locus_tag="KAM546c_46220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005555990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264884037.1"
                     /translation="MTTYWHMQMHPDDKSFAEVHIYSILESKKIIGLGEWNDGESIISS
                     FKNDVKVNDIVAIKNGAKLIALVQVIGGAYTVHDDNSETGWIEHRRPIRVLDWDIWGKT
                     LPQPRGTLVKCVNEVETTKIIREWHTRIAKVLIKRGIATSV"
                     /name="hypothetical protein"
     Typing          complement(2328..2520)
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.423"
                     /coverage="100.0"
                     /evalue="1.73e-88"
     Typing          2511..4042
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.499"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgatttac tttctggcca ccgacgagat tatctatctg gtgatggcct atccgaaaag
       61 taccaaagac agcctgacgg acgccgaaaa atccgacctg aaaaagctga cgaaattact
      121 gaaagatgag gtgtagcatg agtttttttg acgaactgaa agcgtcactc gaagaagccg
      181 tcgagatcaa aaaaggcgtt aaagcgcctg cccgtgtgac acgctacgaa ctggctgacg
      241 tgaaggcact ccgcgcccag ctgaacgtga cgcaggatga aatggcgaag gtgcttggca
      301 ccagcctcga caccatcaaa agctgggaaa cgggcaggcg taacccgacc ggactagcgg
      361 cgaaggtgct ggcgaccata caggccaacc ctaaattctt tcaggaactt gcctcacatt
      421 aaggcgttat gacggaagga ttcgacagaa gccgcagttc gcgtgtaact ttaacggcag
      481 aaaaaaacaa agccccgata atcatgtgct caacttggcg gaagatacat gatcaatcag
      541 ggctaggcat aaaacctaag aaggatatta gcacatgcag agtgcaagac aacagcccgc
      601 cataagcgct ggcgtgagtg actaacccag ctctcgatct gttcaacgac cgcttaccta
      661 acaagccgta tttttctgac gatctgcatt ttggtgtacg catcgcggga aaagagcgcg
      721 caatcctggc gaaatacatt cagttcaacc agcctcacgc catgttctgg ctcggttttg
      781 acgttgaccg tatcggtgcc gccatcgact ggagcgatcg caatgcgccc gcgcctacga
      841 tgaccatcac caatcccgaa aacggacacg cacacctgct ctatgcgttg aaaaactcga
      901 tacgtaccgc accagacgga aaaatgaagc cgctgaagta cgctgctgcc gtagagaacg
      961 cgctacgtaa aaaactggac gctgacgtgg ggtatgcggg gctaatttgc aaaaatccga
     1021 accacggtca ctggaaaatc gccgtctggc agccggaact ctatacgctt gactggctgg
     1081 ccgactcact cgacctgaac gctgcgaacg acaaagaaat cgttgccgat tacggcttag
     1141 ggcgtaactg cactcttttc gataaaacac gcaagtgggc ataccgcgcg attcgtcagg
     1201 gctggcctga gtatgagcag tggctggaag cctgctatga gcgtgccaac gcatataatc
     1261 tgcaattcac atcacctctg gatgaaaaag aggtgggagg tatagcaaaa agcattgcta
     1321 aatggacaca tcaccaacta tcccctgaag gatttgcgga gtatgtatca cgcacacacg
     1381 caccatctat tcaggctgtt agaggccgga aaagcagagg cgggggcagg ccaaaggaca
     1441 gttcagccct cgtaaacacg aagccttggc tgtcccttgg tattagccgc gcaactttct
     1501 atcgcaagca gggaggttag tttttaaact ctcgagctat agcgaacaaa gtcactaaaa
     1561 tcgcaaaaat cactattaag ttcatgacag gcaaggagca aagagtcatg accaactgta
     1621 aaagtagatt tccaacgatt cttgcaatga taaaactaca aacgtaatca ccaataaaat
     1681 caaacatata tatctccact aaggagagtc catgcgataa atatccaagg gggaacgtat
     1741 cccttgtgtc cgaactatgc ttgacgtttt ttacctattt gctaatatac gtttaccaag
     1801 acgataaaag ctaaagatac aaaacaaaaa ccagcccttc atttaaccat aaaaacccca
     1861 aaaaagttaa agtcccattt ataacaataa cacatatttg ctgtaaagca aatatttttt
     1921 ctcttttcgc aaaaaaaaat catccatccc ttttttatgc gtgagacaaa aacaacctat
     1981 atcagataac agccctctct gagggattgt cgttttcttg aacgcaacac ataatctgat
     2041 atactgcacc atactctaag ccagtacaca cagcgtcccc tctatcgagg tgccactgtg
     2101 actggttcag ggggctcgcc gccccccgaa acccctggca tccactgcga aactttcgca
     2161 ctttgggtgc gcatatttct cagcggagtg agaatggaaa agcgcacgag agagatcaaa
     2221 atccgcctaa ccgactcaga gcatcagcgt ctgatcgacc ggtgtgaccg aacccatttg
     2281 gccgaatggc tgcgtcagct cggtctggga gaacacaccg ccagaaagcg ccgtctgcca
     2341 gatgtggcgc ccgaactgct gcgacaggtc agcgggatag gcaacaatct taaccagatc
     2401 gcccgccgcc ttaaccagct cgacggactg aatcccactg agcgtgtgtc cctgctggct
     2461 gtgttaaaca gcctcgaccg caatctcggt gaactgctgg agcagcaccg tgatcgttaa
     2521 aattcactca cggggtgccg gttcgggcag tggccccgtt gattacctgc tgggcaaaga
     2581 tcgccagcgc agtgacgccc gcgtgctgcg gggagacgcc gaacgcaccc gcgaactgat
     2641 tgactgctgt gactttgccc gcgcctacac ctctggcgtg ctgtcctttc aggaagccga
     2701 cattaccgcg cctgaaaagg cgcgtcttat ggacgaatgg gaacagacac tgatgaccgg
     2761 tctggacaaa gaccagtacg cttgcctgtg ggttgagcat caggacaaag gccgcctcga
     2821 gctgaacttc gtgatcccga acatcgaatt acagagaggg aaacggctgc aaccctactt
     2881 tgaccgagcc gaccgtccca gggtcaacgc ctggcagacg ctgaccaacg accggctcgg
     2941 actgcgtgac cccaatgatc ccgcctatcg ccgcccgctg acgcaggcca gcgacctgcc
     3001 gcgagacaaa cagcaggcag cggaaaagct gacgaccggc ctgatgaacc tgatggcaca
     3061 gggcgcgata cgcagccggc aggacgtggt gactcagctc gaaagtattg gcctcaccgt
     3121 ggcgcgggaa accaaaagca gtatcagtat tgccgacccg tccggcggca gaaacatccg
     3181 gctgaaagga atgatttatg agcgagattt taaatttggc gaagggcttc gaggagaaat
     3241 cgaagcagca agcgcaggat accgagcaga gcgtgaagcg cgagttcggg aggctggaaa
     3301 cgtctatcag cgcggaactg caatcaagct cgcagagcat cagcaacgct atccgcgagc
     3361 agaacggcag gctgacgggc atgatcagag ccacagccac caccgcgctg atctggatag
     3421 tggtctgcgc cgcgctcctg attggcatcc tgagcgggat aatctggttc caggggcaga
     3481 tcatcagctc acgcctgagc gacatcgacg gttacagcca gcagctggcg agcctgaaag
     3541 cgaaaagcgg cgcgggggtg acgatctaca gcgacgacag ccagaagaac ggctatctgg
     3601 tagtgctgcc ggtgaaagca cacggcgtgg aaacctacac ttcgacggcg ggcaacacgg
     3661 tggtgaagta caccgcgaaa taaccccgcc agaacgcaca gaaacgcccc tgaacgattt
     3721 tgacccacag gacacagaaa accatgaccg aaatggaaaa acagcttctg agcgccttag
     3781 agagcttaca ggccagctac gaacaacagc atcaggcgtg gcaggaaagc tacagcagtt
     3841 tgcagcacat gttcgagact acctcacagg cactgaaccg cagcgacagg gtgtgtcagc
     3901 acttgagcaa tcaggtgaac agcttgcgcg gtcaggtcga gagcttgagc gacaatgtca
     3961 gccggttaat gcgctcgtaa aacagcacga acaccagctg aaactggaaa agcagcgcac
     4021 ccgctatcat ggcccttcaa tgtgatttgg gatattcata atcaaagagt taaggagata
     4081 taaatgacca cttactggca tatgcaaatg catcctgatg ataagtcatt cgctgaagtg
     4141 catatatatt ccatattaga aagtaagaaa atcatcggct taggtgaatg gaatgatgga
     4201 gagtcgataa tttcatcatt caagaatgat gtgaaggtga atgatatcgt cgcgattaag
     4261 aatggcgcaa agcttatagc gcttgttcag gtcattgggg gggcatacac agtgcatgat
     4321 gacaacagtg aaacaggctg gattgagcat cgtcgcccta ttcgagttct tgactgggat
     4381 atctggggga aaacacttcc tcagccaaga ggaacgcttg tgaaatgtgt gaatgaagtc
     4441 gaaacaacca aaataatcag ggaatggcat acacgtattg caaaagtgct tatcaagaga
     4501 ggcattgcta catcagtttg atacgaaagc tgttgtctga cattgatgca atgagtcttt
     4561 cgatgttgat gtttttggtg tacattgtac cccttaactc aaaaggaatg atacagtgag
     4621 ccatgcgata gagtttatcg aaacgccgat gtttacccgt cagataaagc aaatcgccac
     4681 ggacgacgag ctcaaagagc tccagaaagc gctgattgaa tcaccggaca aaggcgatct
     4741 catcaggcaa accggcgggc tgcgaaaaat cagaatggcg accggcaca
//
