LOCUS       NZ_AP026876             6142 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_3, complete
            sequence.
ACCESSION   NZ_AP026876
VERSION     NZ_AP026876.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026876.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_3"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(6050..6142,1..231))
                     /locus_tag="OO938_RS23295"
     CDS             complement(join(6050..6142,1..231))
                     /locus_tag="OO938_RS23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_264884032.1"
                     /translation="MDKRTKEIKIRLTESEHERLLARCDRAHLAEWLRSLGLGEHVLRR
                     RCVPDVDPALLRQVTGIGNNLNQIARKLHGLDYQQVSLLAVLTSLDRQLSELLETQRDR
                     QNS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(612..1309)
                     /locus_tag="OO938_RS23300"
                     /pseudo=""
     CDS             complement(612..1309)
                     /locus_tag="OO938_RS23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(1449..1652)
                     /locus_tag="OO938_RS23305"
                     /old_locus_tag="KAM546c_46070"
     CDS             complement(1449..1652)
                     /locus_tag="OO938_RS23305"
                     /old_locus_tag="KAM546c_46070"
                     /inference="COORDINATES: protein motif:HMM:NF020541.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthesis protein GlgS"
                     /protein_id="WP_264884033.1"
                     /translation="MEITLQSLDFIAQSFAMMDATTHAHSVDSLTGNMPAEVREEFTTL
                     YNKYLDEITHDTHPATDGIKRD"
                     /name="glycogen synthesis protein GlgS"
     gene            1846..2543
                     /locus_tag="OO938_RS23310"
                     /pseudo=""
     CDS             1846..2543
                     /locus_tag="OO938_RS23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(2764..3666)
                     /locus_tag="OO938_RS23315"
                     /old_locus_tag="KAM546c_46100"
     CDS             complement(2764..3666)
                     /locus_tag="OO938_RS23315"
                     /old_locus_tag="KAM546c_46100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_264884034.1"
                     /translation="MTNQALDLFNERLPRKPYFTDELQFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRPGAAIDWSDRNAPAPTLTIMNPENGHAHLLYALKTAVRTAPDGKMK
                     PLKYAAAVERALCQKLDADTGYSGLICKNPNHDHWQLAVWQPELYTLDWLADSLDLSAA
                     NDKEILPDYGLGRNCTLFDKTRKWAYRAIRQGWPAYDQWLQACHERAGAYNLQFPVPLD
                     ENEVNCIAKSIAKWTYSNFSSDSFLNYVNKTHSAEIQSIRGARSKRKPLLNSDRTLKPW
                     LILNISRRTYYYRKGKGEI"
                     /name="replication initiation protein"
     gene            3948..4187
                     /locus_tag="OO938_RS23320"
                     /old_locus_tag="KAM546c_46110"
     CDS             3948..4187
                     /locus_tag="OO938_RS23320"
                     /old_locus_tag="KAM546c_46110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_064179603.1"
                     /translation="MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRE
                     SEAERWKRANRKAIQELNRITDEHGLLSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            4187..4495
                     /locus_tag="OO938_RS23325"
                     /old_locus_tag="KAM546c_46120"
     CDS             4187..4495
                     /locus_tag="OO938_RS23325"
                     /old_locus_tag="KAM546c_46120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_037427062.1"
                     /translation="MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNG
                     RPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEEVVSVREHRQAIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(4599..4835)
                     /locus_tag="OO938_RS23330"
                     /old_locus_tag="KAM546c_46130"
     CDS             complement(4599..4835)
                     /locus_tag="OO938_RS23330"
                     /old_locus_tag="KAM546c_46130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_037427059.1"
                     /translation="MTETEKLLLSALERLQDDYETRQQAWQASYDSLQTMFTTTSAALM
                     RSDQACQHLSGQVESLRQQVMSLTEAVSRLTRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4896..5381)
                     /locus_tag="OO938_RS23335"
                     /old_locus_tag="KAM546c_46140"
     CDS             complement(4896..5381)
                     /locus_tag="OO938_RS23335"
                     /old_locus_tag="KAM546c_46140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_264884031.1"
                     /translation="MSGILDLAKAFEETSKQQAQRTGESVKSEFVKLEQSISEALKSSG
                     KNISDAIHEQNARQTTLLRRSVTIALIWILVCGVLLIGILSGILWFQGTLIASRLDEMN
                     SYSQQLTDLKAKSGAGVEILTDDTRKNKYYVVLPKSAKGVELYTSKNDKQVVTYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<5428..5850)
                     /locus_tag="OO938_RS23340"
                     /pseudo=""
     CDS             complement(<5428..5850)
                     /locus_tag="OO938_RS23340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
ORIGIN
        1 gtccagacca tgcagcttac gggctatctg attaaggtta tttccgatgc cggtcacctg
       61 gcgaagcagt gccggatcta cgtcaggcac acaccgacga cgtaatacat gttcacccag
      121 accgagcgag cgcagccatt cagcgagatg agcacgatca caacgtgcca gtagtcgctc
      181 atgttcactc tcagtcaggc gaatttttat ctctttcgtt cgcttgtcca tgacactccg
      241 ctgagaaaac tttcgcaccc agagtgcgaa ttttcgcagt ggatgccagg ggtttcgggg
      301 ggcggcgagc cccctgaacc agtcacagaa ggaaccctcg cagaggggac gctgtgtgta
      361 ctggcttaag gtaagggggt agtatgtcag attctttgct ggctgcaaaa aaagccacgt
      421 ctcaacgtgg ctgttatctg atataggtta tctggtgcaa ggttactttt tagggggcct
      481 aggtcgagtt atccaactga tcaacagaca aagagggatt aataacaatg ctgtataatg
      541 tatatatacg cccagatagg acaccttagc gattaacagt acatatataa gcaataggta
      601 atgactccaa cttactgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
      661 agctccaccg attttgagaa cgacagtgac ttccgtccca gccttgccag atgttgtctc
      721 agattcaggt tatgtcgctc aatgcgctga gtgtaacgct tgctgattac gtgcagcttt
      781 cccttcaggc gggattcata cagcggccag ccatccgtca tccataccac gacctcaaag
      841 gccgacagca ggctcagaag acgctccagt gtggccagag tgcgttcacc gaagacgtgc
      901 gccacaaccg tcctccgtat cctgtcatac gcgtaaaaca gccagcgctg acgtgattta
      961 gcaccgacgt agccccactg ttcgtccatt tcagcgcaga caatcacatc actgcccggc
     1021 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     1081 ggccaacgcc cataatgcgg gcggttgccc ggcatccaac gccattcatg gccatatcaa
     1141 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     1201 tacggcagtg agagcagaga tagcgctgat gtcaggcagt acttttaccg ttacgcacca
     1261 cgccttcagt agctgagcag gagggacaac tgatggagat ggaagccacg ggagcacctc
     1321 aaaaacacca tcatacacta aatcagtaag ttggcaccat taccactttt tgcccttttt
     1381 tgaggaacac tttgttcagc cgtttcatgg ccgcagaggt tgataccgcg taagttttca
     1441 gcatcatgtt agtctctttt aatgccgtca gtggcaggat gcgtatcgtg cgttatttca
     1501 tccagatact tgttgtagag ggtggtaaac tcctctctca cctctgccgg catattcccc
     1561 gtcagagagt ctactgagtg cgcgtgggtt gttgcatcca tcattgcaaa cgattgcgcg
     1621 ataaagtcca ggctttgaag tgtaatttcc attttattct ctatccagtt cagttattta
     1681 tcagataaga attttcctag cactgataat tttccactta ccgctttaaa gaaccttgat
     1741 ataaatcaat ttagcttttc aggttattat tatttgttag ttcaaatatg ggtaatggtg
     1801 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctcccgtggc ttccatctcc
     1861 atcagttgtc cctcctgctc agctactgaa ggcgtggtgc gtaacggtaa aagtactgcc
     1921 tgacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac
     1981 accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga
     2041 tgccgggcaa ccgcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa
     2101 aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgatgtg attgtctgcg
     2161 ctgaaatgga cgaacagtgg ggctacgtcg gtgctaaatc acgtcagcgc tggctgtttt
     2221 acgcgtatga caggatacgg aggacggttg tggcgcacgt cttcggtgaa cgcactctgg
     2281 ccacactgga gcgtcttctg agcctgctgt cggcctttga ggtcgtggta tggatgacgg
     2341 atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgtt
     2401 acactcagcg cattgagcga cataacctga atctgagaca acatctggca aggctgggac
     2461 ggaagtcact gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc
     2521 tgaacataaa acactatcag taagttggag tcattaccgg gtcgtaatac ttcagtttct
     2581 tgcgtttaaa aacccaagaa aggaaataag caataactac agataccacc gccgaagaaa
     2641 aaccaagtga atagaatgca ccattgggta gtagcattac caaaacaata tgaatagcta
     2701 tgaacaataa aacaaagtaa caaaaaaaag cacctttgct taatttattg attttatccc
     2761 gaatcatatt tcccccttac ccttcctata ataatacgtt ctacgactaa tatttagaat
     2821 caaccaaggc tttagagtgc ggtctgagtt taataatggt tttctttttg agcgcgctcc
     2881 tctgatagac tgaatttctg cggagtgtgt tttattaaca taatttaaga agctatcgct
     2941 tgaaaaatta ctatatgtcc atttagcaat acttttagct atgcaattca cctcattttc
     3001 atccagaggc acagggaatt gtagattgta ggcaccagcc cgttcatggc aggcctgtag
     3061 ccactggtcg tatgcaggcc agccctgacg aatggcgcga taagcccatt tacgggtttt
     3121 atcgaacagg gtgcagttac ggcctaaacc ataatcgggt aggatttctt tgtcgttcgc
     3181 cgcactcaga tcgagtgagt ccgctaacca gtccagcgtg taaagttccg gctgccacac
     3241 ggcaagctgc cagtggtcat ggttcggatt cttgcagatt aaccccgaat agccggtgtc
     3301 agcgtccagt ttttgacaca gcgcacgctc aaccgctgcc gcatacttca gcggcttcat
     3361 ttttccgtca ggtgcggtgc gtactgcggt tttcagtgcg tacagcaaat gggcgtgacc
     3421 attttcggga ttcatgatgg tcagtgtggg cgcgggggcg ttgcggtcgc tccagtctat
     3481 cgccgcacca gggcggtcaa cgtcaaaccc gagccagaac atcgcgtgtg gctggttgaa
     3541 ctggatatat ttcgcaagaa tggcgcgctc tttcccggca atgcgtacgc cgaactggag
     3601 ttcgtcggta aaatagggtt tgcggggaag ccgctcgtta aaaaggtcga gtgcctggtt
     3661 agtcaatctc gcctgcgctt atggcgggct gttgtttcgc acggttcatg tgctagtatc
     3721 cttcttaggt tttgtgcctg cgccctcgtt aatcatgtcc ttccgccaag tcgagcatga
     3781 ttacgggggc gtcgtctttt ctgccgctta acgttacaca ccccgccgta ttttgtcacc
     3841 tgtcaccgtc agttattgcc tgaagcaaca gaccattacc ttccgggtaa aaaacgcgta
     3901 cactggcaac acgtgctata tacacgtgta aaaatcagga gatcatcatg cccgtatcag
     3961 cgatgcgcca taaaaaaacc gtcagtgtga caatcgagcc tgcgcttttg catcaggccc
     4021 gcgaagccgg gattaatctg tctgccacgc tcaccaccgc cctgaaacat cagatccgcg
     4081 agtcagaagc ggagcgctgg aaacgcgcca accgcaaggc aatccaggag ctcaaccgta
     4141 ttacggacga gcacggctta ctctctgacg atcacaggac gttctgatgc agttcatggt
     4201 ttaccgcaat aacagcaaca accgggctta tccctatctg ctcgatgtac agagcgatat
     4261 tatcgacgaa ctgaataccc gcctggtgat cccgctgttt ctgctggatg aagtgaatgg
     4321 acggcccgcc cgccgcctga ccccggtgct gaatgtggac ggtgaggatt ttctggtcat
     4381 gacacatgaa atggccagcg ttcgccagtc acagctcggg gaagaggtgg tgagcgtccg
     4441 ggagcaccgg caggccatca agaatgcgct ggattttctg ctcgacggtt tctgagttta
     4501 cctttccatc ccgtgatcgt gtccccggct gcgtccgggg gcaaatgagc gcgttttctg
     4561 tcgttcctgt ttctgcgcca gctccctgtc cagtctggct acctgcgcgt caagcggctg
     4621 acggcttcgg ttaagctcat gacctgctgc cgcaagctct ccacctgccc gctcaagtgc
     4681 tgacacgcct ggtcgctgcg catcagcgcg gcggaagtag tggtgaacat ggtctgcaag
     4741 ctgtcgtaac tggcctgcca tgcctgctgt cgtgtctcgt aatcgtcctg taagcgctct
     4801 aacgcactca gcagcagttt ttcggtctcg gtcatggatt tgcttgtcct tatcgctaaa
     4861 aacgccgtat ggcgcttctg tggcaattga cggggctatt tcgcggtgta ggtcacgacc
     4921 tgtttgtcgt ttttcgaggt gtacagctcg acgcccttcg cgctcttcgg cagcaccacg
     4981 taatacttgt tcttccgggt gtcgtccgtg agtatttcca cccccgcccc cgatttcgct
     5041 ttcaggtcgg taagctgctg gctgtagctg ttcatctcgt ccagccgtga ggcgatgagt
     5101 gtcccctgaa accacagtat ccccgacaga ataccgatca gcagcacacc acaaaccagt
     5161 atccatatca gcgctatcgt gaccgagcgc cgcagcaggg ttgtctgccg ggcgttctgc
     5221 tcgtggatag cgtcgctgat gttcttcccg cttgatttca aggcctcgct gatggattgc
     5281 tcgagcttca caaactcgct cttcacgctc tccccggtac gctgtgcctg ctgcttcgag
     5341 gtctcctcga aggctttcgc taaatctaaa atcccgctca tataacgctc cttttaaccg
     5401 gatatttcgt ccaccgtccg ggtcggcaat gctgagactg cttttcgttt ctcgcaccac
     5461 tgtcaacccg ccgttttcca gcgccctaac cacatcctca cggtcgcgga tcatcccctg
     5521 ttccaccatc gtttccagcg agtccgtaat ggtctgcgcc gccttctgac ggtcgccggg
     5581 aaggtcattt cccggtgtca gggttcgcct tcgggcggga tcatgagggt catcaagatt
     5641 caggcggtca ttggtcagag tctgccaggc gttgaccctg ggacggtcag cccggtcata
     5701 gtagggttgc aggcgtttcc cgctctgcaa ttcgatgttc gggatcacaa aattcagctc
     5761 cagcctgtcc ttgtcccggt gctccaccca aaggcaggcg tactggtcgc catccagacc
     5821 gggcaacagt gtctgctcga actcctccat cagacgctct ttctcatcgt cagggaggtt
     5881 ttcttcccgg aacgacaaca ccccggaggt gtaggcgcgg gcaaaatcac aaccatcaat
     5941 cagttgcctg atacgctcag gctcaccgcg cagcaccacc gctttttccc tgtctcgttc
     6001 tttccccagc aggtaatcca ccgggccact gcctgcaccc gcaccccggc tatgaatttt
     6061 gacgatcacg ctgtgtctcc agaagctcag ataactggcg atcaagactg gtcagtaccg
     6121 ccagaagaga gacctgctga ta
//
