LOCUS       NZ_AP026875             6724 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterobacter roggenkampii strain KAM546 plasmid pKAM546_2, complete
            sequence.
ACCESSION   NZ_AP026875
VERSION     NZ_AP026875.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00535933
            Assembly: GCF_025999915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Complete genome sequence of colistin-resistant Enterobacter spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Maehana,S. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 84x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:21:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,425
            CDSs (with protein)               :: 4,425
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 59 of 181
            Pseudo Genes (incomplete)         :: 116 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 16 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026875.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="KAM546"
                     /db_xref="taxon:1812935"
                     /plasmid="pKAM546_2"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(6512..6724,1..782))
                     /locus_tag="OO938_RS23255"
                     /pseudo=""
     CDS             complement(join(6512..6724,1..782))
                     /locus_tag="OO938_RS23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /name="IS110-like element IS4321 family transposase"
     gene            complement(1213..1425)
                     /gene="cspG"
                     /locus_tag="OO938_RS23260"
                     /old_locus_tag="KAM546c_45990"
     CDS             complement(1213..1425)
                     /gene="cspG"
                     /locus_tag="OO938_RS23260"
                     /old_locus_tag="KAM546c_45990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="WP_001548019.1"
                     /translation="MSNKMTGLVKWFNSDKGFGFITPADGSKDVFVHFSAIQSNDYKTL
                     DEGQKVEFSIENGAKGPAAGNVVGL"
                     /name="cspG"
     gene            complement(1813..2510)
                     /locus_tag="OO938_RS23265"
     CDS             complement(join(1813..2261,2261..2510))
                     /locus_tag="OO938_RS23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_230683675.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDQVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(2591..3127)
                     /locus_tag="OO938_RS23270"
                     /old_locus_tag="KAM546c_46020"
     CDS             complement(2591..3127)
                     /locus_tag="OO938_RS23270"
                     /old_locus_tag="KAM546c_46020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047741458.1"
                     /translation="MKKVYKAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVISSEINYDLRSVSAENSSLDVNRVMLQFAEKVKDDDFSKVYLSYRGKDKFY
                     LKGGYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(3949..4371)
                     /locus_tag="OO938_RS23275"
                     /old_locus_tag="KAM546c_46030"
     CDS             complement(3949..4371)
                     /locus_tag="OO938_RS23275"
                     /old_locus_tag="KAM546c_46030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004216558.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4371..4562)
                     /locus_tag="OO938_RS23280"
                     /old_locus_tag="KAM546c_46040"
     CDS             complement(4371..4562)
                     /locus_tag="OO938_RS23280"
                     /old_locus_tag="KAM546c_46040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5043..5192)
                     /locus_tag="OO938_RS23285"
                     /old_locus_tag="KAM546c_46050"
     CDS             complement(5043..5192)
                     /locus_tag="OO938_RS23285"
                     /old_locus_tag="KAM546c_46050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017880354.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5754..5885
                     /locus_tag="OO938_RS23290"
     CDS             5754..5885
                     /locus_tag="OO938_RS23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047741455.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGKQDVREM"
                     /name="hypothetical protein"
     Typing          5348..5863
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.871"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aatgtcgtcc gacctcccgt gctgtactgt cgaggcacta gccctgttgc cgccgcaaag
       61 tcacgactgc tggcgtactg cttcccgtcg ccaatctcag ttgaaatagt actcgctgtc
      121 agtgttccga cgcagggaat gctcagcaag cgctgtccaa tctcatcttc gtccaacttt
      181 cgtttcaact gggattccaa atctttaatc tgctcaacaa gatagtgata atgctgttgt
      241 aatttcagca ataactggct gaggtataga ggcaaactat tgtcctcaag aagggtactc
      301 agtcggctaa taacggcagc tcctcgtgga acgctgatgc caaattccag cagaaaagca
      361 tgcatctgat tggttgtttt taccttatcc tgaaccaggg attcacggac acgatgcagc
      421 gcacgcattg cctgctgaga ttcagttctg ggctgtacaa aacgcataga cggacgcgat
      481 gcagcttcac aaatagcttc ggcgtcgaca aagtcgtttt tgttactttt aacgaatgga
      541 cggacaaatt gtggtgatat cagcttagga aaatgcccca actcttccaa cttgcgtgcc
      601 ataaagtgag agccaccaca ggcttccatt gcgatggttg tagcggggca tgtcgccaaa
      661 aattcgatta actttggccg tgtaaatttt ttacggtaaa cagccttgcc gcgacgatcc
      721 tggcaatgaa tatggaaaga gtttttaccc agatcgatac caatgagcgc aatgttttcc
      781 atgatagttc tccgaatgaa agcctatcct cagcatagta ccgggaagga gggagtgacc
      841 atctcattaa atatcctacg ttaagcctgt tttttaagca ggcatgacag gcagctatga
      901 gcgaggagca gacatccttg acctgtgaat catgaatatt attatctctc aggcttatta
      961 aacagactta gggaggactg gtgttgggct ggaaattact aatcggcttt ctttttttca
     1021 ccatcattgg gtttgcacta tttggtttca ttttttcact ccagcaactg cagtaaaatg
     1081 atcgtccccc ctgttgcagg gccatccatg aaacacaaaa acccgctcat ggcgggtttt
     1141 ttatcacgtt cacttatctg ctcaggccag tgccgtcatc gctgtcgtaa gcgtctgtgt
     1201 cgttatcggc aattaaagcc cgacaacatt tccggcagcc ggacctttgg caccattctc
     1261 aatcgagaac tcaaccttct ggccttcgtc gagcgttttg taatcgttgc tctgaatggc
     1321 agagaaatga acaaaaacat ctttgctgcc gtcagctggc gtaatgaagc cgaaaccttt
     1381 gtcagagtta aaccatttta ctaaaccagt cattttattc gacatagata cttcctttaa
     1441 ttttcttgag ccacacgttg tggcgaacat ggcctgcttg cagatgagga cttattgggc
     1501 acttaggagg aggctcatgc agaagagtat ctaaggataa cgcttgaaat gaggactgct
     1561 ttactaaaac tgctttcata aggtctgtat gacaaaccga tagcgcatag tggactcata
     1621 tgagttgata agcaagatta tttttgtcag taatctgttg ggaaaactta caattcaaag
     1681 aggtcagtaa tcttaaaagc cctgaccatt gcagccaggg cttttaaggt actacagact
     1741 tcagatttaa cattaatgga cttaataaag tgatactgct atttatactg ctttcaaggt
     1801 aatgactcca acttattgat agtgttttat gttcagataa tgcccgatga cttggtcatg
     1861 cagctccacc gattttgaga acgacagtga cttccgtccc agccttgcca gatgttgtct
     1921 cagattcagg ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa cgtgcagctt
     1981 tcccttcagg cgggattcat acagcggcca gccatccgtc atccatacca cgacctcaaa
     2041 ggccgacagc aggctcagaa gacgctccag tgtggccaga gtgcgttcac cgaagacgtg
     2101 cgccacaacc gtcctccgta tcctgtcata cgcgtaaaac agccagcgct gacgtgattt
     2161 agcaccgacg tagccccact gttcgtccat ttccgcgcag acaatcacat cactgcccgg
     2221 ttgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa aaccgtgttg
     2281 aggccaacgc ccataatgcg tgcactggcg cgacatccga cgccattcat ggccatatca
     2341 atgattttct ggtgcgtacc gggctgagag gcggtgtaag tgaactgtag ttgccatgtt
     2401 ttacggcaat gagagcagag atagcgctga tgtccggcag tgcttttgcc gttacgcacc
     2461 acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac gcaagcacct
     2521 taaaatcacc atcatacact aaatcagtaa gttggcagca ttaccgtcaa tgtatactga
     2581 tcgcttcaaa ttaatcgctc atgcttttaa tgaaatcatc taagtaccaa gccttagaga
     2641 aatcactaag atcttccatc tgttttccca ttacacccag aagcccaccc tcccaaacac
     2701 tataagctct ctctccattt aacatataca cattttccgg aattgttctt aatgtataca
     2761 cggggttttg aataccatat tcctgcccaa gagttttaaa atacccccct tttaagtaaa
     2821 acttatcttt ccctctgtaa ctaagataaa ccttactaaa atcatcatct ttcacttttt
     2881 cagcaaattg cagcatcacc ctattaacat ccagtgaact attttctgca gaaacagaac
     2941 gaaggtcata attaatctcg gaagagatta caaaccactt ataataaacc caaacgctaa
     3001 taccatcatt ccttggatct tctttcagaa cctcattcat gtgcctttga agaaaaatat
     3061 agttggcaga taaaacacca ccacatagga gtactattaa tatgattgct ttatatactt
     3121 ttttcatttc tataatccat ctcaactgct ctaaagttta ccctgagatt tttaagctaa
     3181 ggtgccataa gttagcaagg aattaagcat tgatttacca taatgcggca ttacgcgcac
     3241 cgctaaaacg gcctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc
     3301 cgaaaacccg ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcgcaa
     3361 gtgcatagcg cggaaattga caccattcag ccaggggaag tcctgtttcc ggcgttcttc
     3421 ctatccgaaa acgttgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg
     3481 cagccggaaa accggttttc agcacgttca ggaatcgggt tcgtcgacgg ccggcaatca
     3541 cgttatggca agtcgccgga cgactcacca cacgattcac cggcgattcg actcgtgact
     3601 tgactctaga gtcaccacgg tcaaaaaaac gccaaagcga cgattcgact ctagagtcaa
     3661 gtcgtcataa gttactgata gtgtggagtg agtcgtcgat gactctatga cgagtcgtcg
     3721 gcaagtcgta cacgaatcac cgacgacttg gattgacctt acagaaggta atactgcgta
     3781 atttaataac aaccaactaa ctgatattaa tgagttatct gcaaaaattt aattttgcgt
     3841 ggggtgtgga tatttttagt gtgaggagcg ttcagagatc tggcgatttt tgggaaggtt
     3901 caggaacgcc ccgggatggc cggagaggaa aactcaagca tggcgggatc aggatttttt
     3961 ccagaatttc caccacgggg aatggggtgg tgaagaaggt tcgtgcttgt gctccagaag
     4021 ctgcatagcc tgacgaaggg aatcaatatg ctcatctcga gccttaacag cccctttaag
     4081 accatcgttt tcagcttcaa gtaaagcgat tctatgttgt aaatcatgca aaccaacgct
     4141 gttaatacct gtagattctg cgtcactttt tacatcatat tttacagcat cgattacacc
     4201 atcaagagaa ccaaacactc taataagctc tgatgtatca ataactttaa tacccttccc
     4261 gtgctttaca acagaggtgt aacttaactt accactgtta atcatcttgt aaatagtagg
     4321 gcgactaacc ttaacaagcc gtgctgcttc actaatagac actctggcca tcagttacca
     4381 tcctgtaatt catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg
     4441 agtttctcac aaaggtcggc ctcagcatcg agatcaagct cgttaagctt ctccagcagg
     4501 agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc
     4561 ataagggacc tcagagcatc aatgctcagt gtaaaaccgt taagggggtc ggatgtacga
     4621 atggttcaga gaacggatct gaggtaatcc tgtagttatt ttgtagttta tctgtagtca
     4681 tcgcactaaa aatcaggatt aagtcatcag cttttgtagt ctttctgtag tttacctgta
     4741 gtccggaaaa ataatccgtt catgcgagca tgaacgaggt cgttaatgac gtttttttgt
     4801 caccgacgcc ccttaacctc cgcagatcac ccggccagaa tgataatttt ttggccctta
     4861 aatggtcata cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaaagtaa
     4921 aaactgaaat ctccgtttat ttatgtaata cgcaacgtat tacaatcacc tgtaattaca
     4981 tgaaaataaa gaaataaaat gtaataccgg cgtattacca cgtacgcggg caagcgaaaa
     5041 agctataacg cttcccggcc gacaggcggc ccggaatcca caggggcatg caggttctct
     5101 tcagtaacct gcgccattaa atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc
     5161 gaatccagtt gttcatcata ttcagatggc atggggtgat atccagtagc tgagaggttg
     5221 ttagcggggt tctgggcgca gccctgaacc agtcgagtag cactagctga gtgtatacgg
     5281 gctcaatatg ctacggtgga gtaacacgtt atgaaagtgc ttcatgtgac agaggaaagt
     5341 gcagcggcgg ggcgtacggg agaaaatgga agcccgggga tatgacgcct cctcgctcac
     5401 tcggtcgcta cgctccggcc gttcggctgc ggcgggcgtt acctgctcac gaatgacgag
     5461 gtaacgagat gaagctgagg tcaagaaagg ccggaaaaag gatgcggcat tgccgttttt
     5521 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     5581 aaacccgaca ggactataaa gataccaggc gtttccccct ggtggctccc tcgtgcgctc
     5641 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca
     5701 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     5761 aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5821 cggaaagaca tgcaaaagcg ccactggcag cagccactgg taaacaggat gtgcgagaga
     5881 tgtagtaaga gttcttgaag tgatggcctg agtgcggcta cactgaaagg acagatttgg
     5941 tgacatgtct cgcacaagac tgttaccgcg gttagaaaat ctgccaggat taactaacct
     6001 tcgaaaaacc acctgccagg gtggtttttt cgtttacaga gcaagagatt acgacgcaac
     6061 agaaaggatc tcaagaagtt ccttgctgta ttgctactat cctagagttc tgacccatta
     6121 ggggcattat cagatgacgg ccttgtaatc cacggggagc acgtcactta tactgtacaa
     6181 aagtatgaag tagtgcaaaa tgcatttggt tttatagtat tatgatgcgt atagggggtc
     6241 cgcttggaaa acggaatcta tggtcactcc cgtttttgca acaccgattt tgacgataaa
     6301 ttggcttgct tgaatctatc cggcgtctga atgggatttt attcccgcgc ctcgatgagt
     6361 tccgcgcctg atgaacctcc agaaaatata cggcttcaat gagcctttcc gttttacagg
     6421 ttcctcaaca ggccggtggg ccgttagtat catcaatatc agtattcgca aaaccagatc
     6481 agtgattctt taaaccggtg tatttctgcc gttatgctac ataagtttgc tgtcgtgccg
     6541 ttagggccca ggctattctg gccagcttgt ttgccagagc acaagtgacg acaaagttgc
     6601 ttttcctaca cagtagatcc ctgacccaat cggccaattt gccagactgg tgttccagtt
     6661 tttgtatgaa taccctggca cattgaacca acaaagttcg gatctttttg ttacctcgct
     6721 tact
//
