LOCUS       NZ_AP026778             6308 bp    DNA     circular CON 27-NOV-2023
DEFINITION  Enterococcus faecium strain NUITM-VRE1 plasmid pNUITMVRE1_5,
            complete sequence.
ACCESSION   NZ_AP026778
VERSION     NZ_AP026778.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00533729
            Assembly: GCF_025998395.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Complete genome sequence of Enterococcus faecium NUITM-VRE1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Suzuki,M., Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/27/2023 03:20:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,228
            CDSs (total)                      :: 3,138
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 65 of 163
            Pseudo Genes (incomplete)         :: 91 of 163
            Pseudo Genes (internal stop)      :: 36 of 163
            Pseudo Genes (multiple problems)  :: 25 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026778.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VRE1"
                     /db_xref="taxon:1352"
                     /plasmid="pNUITMVRE1_5"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            510..1460
                     /locus_tag="OO969_RS16005"
                     /old_locus_tag="NUITMVRE1_31600"
     CDS             510..1460
                     /locus_tag="OO969_RS16005"
                     /old_locus_tag="NUITMVRE1_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_164450254.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            1481..2014
                     /locus_tag="OO969_RS16010"
                     /old_locus_tag="NUITMVRE1_31610"
     CDS             1481..2014
                     /locus_tag="OO969_RS16010"
                     /old_locus_tag="NUITMVRE1_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            2030..2200
                     /locus_tag="OO969_RS16015"
                     /old_locus_tag="NUITMVRE1_31620"
     CDS             2030..2200
                     /locus_tag="OO969_RS16015"
                     /old_locus_tag="NUITMVRE1_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            2650..3183
                     /locus_tag="OO969_RS16020"
                     /old_locus_tag="NUITMVRE1_31630"
     CDS             2650..3183
                     /locus_tag="OO969_RS16020"
                     /old_locus_tag="NUITMVRE1_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            3195..3398
                     /locus_tag="OO969_RS16025"
                     /old_locus_tag="NUITMVRE1_31640"
     CDS             3195..3398
                     /locus_tag="OO969_RS16025"
                     /old_locus_tag="NUITMVRE1_31640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            3720..4100
                     /locus_tag="OO969_RS16030"
                     /old_locus_tag="NUITMVRE1_31650"
     CDS             3720..4100
                     /locus_tag="OO969_RS16030"
                     /old_locus_tag="NUITMVRE1_31650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4082..4996
                     /locus_tag="OO969_RS16035"
                     /old_locus_tag="NUITMVRE1_31660"
     CDS             4082..4996
                     /locus_tag="OO969_RS16035"
                     /old_locus_tag="NUITMVRE1_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4956..5234
                     /locus_tag="OO969_RS16040"
                     /old_locus_tag="NUITMVRE1_31670"
     CDS             4956..5234
                     /locus_tag="OO969_RS16040"
                     /old_locus_tag="NUITMVRE1_31670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            5241..5930
                     /locus_tag="OO969_RS16045"
                     /old_locus_tag="NUITMVRE1_31680"
     CDS             5241..5930
                     /locus_tag="OO969_RS16045"
                     /old_locus_tag="NUITMVRE1_31680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          512..1460
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.057"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4083..4993
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caagactgtc ttaaaacgac ttagacgagc tatcagaggc ttcccccacc tccgttactg
       61 acttgttttt agcaaaggag cctttaggcg actgcgcagt acaatgtgag agcatagcga
      121 tcacactttt ttagcgaccg tagggagcaa ggttttgaat tggcagtttt gaaaaatgcg
      181 cgaacctgtt ggtagaaaaa agaagtggtt tttaggcggg aaattttgtc accctctttt
      241 ataatctctt tttaaacctt tttttaaacc tctttaccgt cggctgtgtc cttactcctt
      301 caatggatac agcgttttat aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      361 ttttaaacta cattttgtct gcttttaaac tacattttgt ctgcttttaa actctttttt
      421 tgcactcacg tatgttataa tatgcattat cacagacaaa acgttgttta taacgtctgt
      481 atccattgaa ggagtaagga ggaatatctt tggcaaatga aatcgtgaaa taccatcatg
      541 aattaaatac cattccgttg cggaaattta caccagttga aatgaatcta tttttctcta
      601 ttgtgtctag aatgcgcgaa aaaggcgata aaacagtccg tcttactttt gaccaactaa
      661 aagatttaag taattataaa gcaactgcta ataatcgttt cattaatgat ttagaaatga
      721 cttatgataa attaatggat ttacgttttg gtaaaagaag tgctaatggg ctcgatatag
      781 atagattcgt tatgtttagt gagtttaaaa tacgtggaac tgctaaagat ccatatgtag
      841 acatacaagt ttacgaaaaa gctttacctt tactaaacaa tttagatgag tgggttcgct
      901 attcactcca acaatttaac gaattacaaa gtagctatgc aaagactatg tttcgattac
      961 taaaacaata tcgaactaaa ggttttgctt atttttctaa agaggatttt catgaactat
     1021 tagatattcc aaaaagttac tggaacaacc aagcaaacgt tgataatcgt gttataaagc
     1081 ctatacgaca ggaattaacc gcaatcttca aaggattatc aatcaaaaag aaatacggaa
     1141 aagggcgagg aaaacctgtc gttggctatc aatttacgtt taaaccagaa atcaaaaaag
     1201 cagatgattt taataaacat gcaccaacca aagaagacca aaataaagca ttggctttag
     1261 ctgataaaca aaaagaatac gaagaacctg ctacaacaat taacgaaaaa atcaaacaga
     1321 agaaacgaga acttgaactc ctccaacaaa tcaaacaact agaaaaagag aaagaagctc
     1381 ttgaaaaaca aacagagtcc gcactttcga aaaaagagaa aaaaagttta ttatctgatt
     1441 tagataattt attccagtag caacctagga ggcaaccagt atgcaaccac aagaaacctt
     1501 tacaataaag gaattgtcag aactttttaa gatgagtagg caagcaatat cgaaacatat
     1561 ccaaaaatta gattcttcca tgatagctaa aaatgagcgt ggttataagg ttgtattgcg
     1621 ttctggagta ttgcaacttg ctcgcaactt aggcaaccaa atgttgctag ataggttgca
     1681 cgagtacgaa caagacaacg ataaaaatgt taatatacca agcaaaaacg tggttacaaa
     1741 caagttgcta gatcagttgc gtgaagaaaa tgaattttta cgagaacaac ttgccctcag
     1801 agagcaaaat aattctcata tacaaaaact actcgaccag caacaacaac tcaccctaca
     1861 agccaacaaa caaatcgaaa aactgcaaga aaaactacag ttgacttata cagaagaagc
     1921 tccagagaat gactcaacga ctctttctga agcaacagaa acaatagaca aacaaattga
     1981 tactgaaaaa aaatggtggc aactttggaa ataactggag ggaacattca tgacaagaaa
     2041 aaatcttcta ttcggttcta ttttactagg aattctagga ttcagtccga tgttattgtc
     2101 agtgatcaat aaagggaata aacatggtga attgaaattg aatccaaatt attacaacag
     2161 tcctaacgaa gaattgtttg taaaagaaac agataaataa taaaaaagac tgctctttcg
     2221 agcagccagt taaaaaataa tgagtgttta ttcttgaaaa agcaacgcgc ttatgaagcg
     2281 ctaccaaaaa tttatcatat tttattaaaa ataacaaccc agtaaaaaat gactggtaaa
     2341 aattcacaaa gaaacggaaa gaagcaaccg cagaaaatca agcttaaaaa gagcttttga
     2401 cctagaccaa tcgaaaatca caaagtgagc ctctgtatcg gttgatacaa gggctacttg
     2461 gcatagtgta cattatgtca acaagaattt aatataacat attttcctaa tgcattctaa
     2521 ctataccaca ttatcaaaaa tataaattat ctttactaaa taaaatattg acaagcctat
     2581 accttttgtt ttatgatttt cacaaagaaa acgttttcta tatatttttc tgtatcagga
     2641 ggaaattcta tgaaaaaatt tactttaggg gtagctttgt taactattgg ggcagttgtt
     2701 ttaccgtcaa ctactatagt ttatgcttca gaaaattccc aagtggtatc attaaacgaa
     2761 aacaatgcta ctgaaaatgt agttattgag tacactgaag atggtatggt cgtaactaaa
     2821 tatgaggatg gttatagtgc ggaagatttt gcaaattctt cagatatcat gacaagagct
     2881 gcagttgcaa gatggggcaa aaaggaatat acaaatattg ctgtaaccac tggagtagct
     2941 gctaatgcta ttaatgctgc cttttgggca ggagggaaag ctagcacttc tatgtttggt
     3001 atcccaggtt gggcaattgg tgctttgtta acaggtgcaa gttggactaa aaaaggtgct
     3061 tctccaggga atgcagttgc aaaagaatgg gacaaaaatg gtaatggctg gattggattt
     3121 tattaccaaa ggggatatga tgcagctgga agagttgtag ctactagata ttcaacgtta
     3181 taagttaagg agtgatgttt ttgatatcta aatcattaaa atcattaaaa acatttacta
     3241 aaaagaatcc gcaaatagct actattcgta tttttatatt tgctatagct gttgctataa
     3301 ttgttaatat gctaagcgga gggcaattat acaaatctgg tttattttta gtgttaatct
     3361 tagttgtctt agacataata aagtctaaaa aaaaataaat cgatacattt tagaagaata
     3421 gggttgtttg aatattacaa agcaacaatt tcaccttcgg tgaaataagc gaaactttac
     3481 aaaaagtggc tagcaaatgc tagccttttt ttgtattgtg gagcgtggat tttgaggggg
     3541 attcaagggg gagcttgcgc tcctttgaca agcggttatt tgcaccaaat acggaggtat
     3601 ttggctagca aatatagagc ttgccaaacc cttttagtga gctttactca ttgtaccttt
     3661 ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg tactgaaagg gtgggactta
     3721 tgagcgaaca acatcaaaat ttggctagcg atcattctaa aaaatatacc tatcgatccg
     3781 agccaaaaca aatcagtttt cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag
     3841 ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc
     3901 tggtgacacc gaaagtggcg ccagacattg cccaggcaat cgcgcgcgat ttagccaaag
     3961 ctggagggaa tatcaatcaa attgccaagt ggtgcaatac acaccaacac gacgttgctc
     4021 ctgatgatgc tcaacgttta tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga
     4081 tatggcaaca gttaaagtaa gtcgaaccac ttcatgtagc cgagcaatca attacgcaga
     4141 accacgagca accgtcaaaa caggaataaa ctgtgacatt gattatgcca aaagtgaaat
     4201 gaaacaaata cgcacgcttt atggaaaaga cgatcgtgta caagcacact tattgattca
     4261 gtcctttcga ccaggagaaa ttaccgctga aaaagccaat caactagggg cagagtatgc
     4321 gaaaaaaata gcccctaacc accaaatcgc gatttacaca catacagaca aagaccatat
     4381 tcataatcac attgtcatta actccgttaa tcttgaaact gggaaaaaat ttcaagccca
     4441 tgggcaagca tttttaaaca aatgttatga cctaaatgac gagatttgtc ttgaacatga
     4501 cttaacaatc acggaacgag gaaagaaaac cgaaaaacga accatgtctg aaattaaatt
     4561 aaaagaaaaa aatgaaccgg tttggaaaga cgaaattcgg tttgcgattg atcaaacaat
     4621 gaaaaacaaa aaaactcgaa cgtataccca attttgtgat tcacttaaaa cattcggcat
     4681 tcattgcttg aatcgcggaa aaaattttac ttacgaattg gtccacaaaa agaaaaaagt
     4741 acgatccaac aaactaggaa aggattacga aaaggagacg attttacatg agctggacag
     4801 acgagaacga acctacaatc aacaacgaac caacgagcta cgaagagctc gagctagcat
     4861 tcgcgaaact gcagaagaag tacgaagaga acaacgaact tttacgcctg aacaatcgga
     4921 aattagaccg actcgaagaa accatgaaca acacgttgaa cgaggaacag accaccaaca
     4981 agaattatcg ctctaaaacg ttaaaagaca tcgaaaacga aataaagaga caacgtgcct
     5041 ttcttgcgcc ttatacccaa gaaaggatac aaactacgat ggattcacaa gtcaaaaaac
     5101 tccaccaagc gctattagac cagcgaaacc aagaacaaaa aaaggcttta attcaacgat
     5161 cgatggatat tcttgccccc ttatgttgct tagtactaac attattagcc ttttggttgg
     5221 tatggggaga ataaacagac atggcattct atttacccta tctactcatt tctgccagta
     5281 ttttaggaat tatttggctt agctataagg cttatcaaac aagatattca ttaaaaggct
     5341 ctaaaattcg atttaagcga tttttattgg tcggggttat atttgcctta tcaatcgttt
     5401 taagtggttt attcggcgtt ttagagggta ataaacgtgt ttcaagaagc attttattag
     5461 gggatatcac tcaaaagtat gaagcagcat gtgataaaaa gaaaaaagag caagcattag
     5521 cacaaaaaat ggaggaattt accgcttgtt atgaggacat gaacaatatt tttgtggatc
     5581 aagagaaacg tttaacagat aaaaacatga aaaaactcac tcgtttttat caaaacttac
     5641 cagaaaaaca gcaaaaagag gtacaagaaa aatatgagca agtaaaaaaa gacgtgcaat
     5701 attttaaaga tacgcagaca gaagaagctt gttgtaactt atttagcgat acaatccctt
     5761 tttcaaccag tgaacaagaa cgaaaagaac ggcaacaatc ggtcacatac gaacgttatg
     5821 aaacactgct tcagcaagca acaaacatac aaaatccaac gaaaaaagaa acggcattaa
     5881 attatctaga aagtgtcaag gaatggttgg atcagcaaca acaaaattga atttgattag
     5941 caatcatgtt aaactgaacc tgcaaatgat caaacattag atacacgaaa gccccagaga
     6001 ggtcggattc tctggggctt tttttcaggt tgatgtcgcc tttattgatt ttacttcttg
     6061 tttcggttat tcaaccaata actgaataaa gagacacaaa cttctacaag aagggggcaa
     6121 atgaaccgtt caaacattag atacacctcc ttccagtcgc aagactgtcg gataggcgac
     6181 aagttattat accttacaaa ataaaatctg tgtggtgttt ttaagtatcc cttattttcg
     6241 ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat aaggtttttt
     6301 acccaaga
//
