LOCUS       NZ_AP026670             9013 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Enterococcus faecium strain GK961 plasmid pGK961_3, complete
            sequence.
ACCESSION   NZ_AP026670
VERSION     NZ_AP026670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00466817
            Assembly: GCF_026073915.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 05:43:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,126
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 123 of 305
            Pseudo Genes (incomplete)         :: 166 of 305
            Pseudo Genes (internal stop)      :: 91 of 305
            Pseudo Genes (multiple problems)  :: 64 of 305
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026670.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="GK961"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pGK961_3"
                     /geo_loc_name="Japan"
                     /collection_date="2015"
     gene            complement(join(8252..9013,1..401))
                     /locus_tag="OQH70_RS16045"
                     /pseudo=""
     CDS             complement(join(8252..9013,1..401))
                     /locus_tag="OQH70_RS16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(807..1061)
                     /locus_tag="OQH70_RS16050"
                     /old_locus_tag="EfmGK961_31670"
     CDS             complement(807..1061)
                     /locus_tag="OQH70_RS16050"
                     /old_locus_tag="EfmGK961_31670"
                     /inference="COORDINATES: protein motif:HMM:NF013677.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(1254..1529)
                     /gene="prgO"
                     /locus_tag="OQH70_RS16055"
                     /old_locus_tag="EfmGK961_31680"
     CDS             complement(1254..1529)
                     /gene="prgO"
                     /locus_tag="OQH70_RS16055"
                     /old_locus_tag="EfmGK961_31680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(1501..2454)
                     /locus_tag="OQH70_RS16060"
                     /old_locus_tag="EfmGK961_31690"
     CDS             complement(1501..2454)
                     /locus_tag="OQH70_RS16060"
                     /old_locus_tag="EfmGK961_31690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            3066..4559
                     /locus_tag="OQH70_RS16065"
                     /old_locus_tag="EfmGK961_31700"
     CDS             3066..4559
                     /locus_tag="OQH70_RS16065"
                     /old_locus_tag="EfmGK961_31700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            4694..4981
                     /locus_tag="OQH70_RS16070"
                     /old_locus_tag="EfmGK961_31710"
     CDS             4694..4981
                     /locus_tag="OQH70_RS16070"
                     /old_locus_tag="EfmGK961_31710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="WP_265145944.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANEGSVAKF"
                     /name="type III secretion system protein PrgN"
     gene            5035..5715
                     /locus_tag="OQH70_RS16075"
                     /old_locus_tag="EfmGK961_31720"
     CDS             5035..5715
                     /locus_tag="OQH70_RS16075"
                     /old_locus_tag="EfmGK961_31720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            5913..6518
                     /locus_tag="OQH70_RS16080"
                     /old_locus_tag="EfmGK961_31730"
     CDS             5913..6518
                     /locus_tag="OQH70_RS16080"
                     /old_locus_tag="EfmGK961_31730"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            6534..7106
                     /locus_tag="OQH70_RS16085"
                     /old_locus_tag="EfmGK961_31740"
     CDS             6534..7106
                     /locus_tag="OQH70_RS16085"
                     /old_locus_tag="EfmGK961_31740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002303206.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(7579..8151)
                     /locus_tag="OQH70_RS16090"
                     /old_locus_tag="EfmGK961_31750"
     CDS             complement(7579..8151)
                     /locus_tag="OQH70_RS16090"
                     /old_locus_tag="EfmGK961_31750"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_073120187.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREV
                     EQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGF
                     YNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="IS3 family transposase"
     Typing          3067..4559
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttctgat tcgtctttct ttggttttga ttgataataa taggactgac gtgatagacc
       61 taatattctg cacatcgctg atatagggta aagatgctta ttcgcatcga ttacttgtct
      121 cttcgtccga atatcagcgc tgcttgcttt aaaatatcat tttccatttc taattgctgg
      181 tttcttttac gtagttctaa caattctttt tgttcaggcg taagattatc tttttctttg
      241 aatgaaccac tcgttttaaa ttgctttacc catttgtcaa atgctgaagc cgttagttca
      301 tattctcgaa tgatttctac acgtggcttt ccagctaagt aaagattgac gatttgttgc
      361 ttgaattctt gtgaaaaagt tcttcgtgtt ctcttagaca taaaaattcc tcctggtatg
      421 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccatgat
      481 cggtttatta gacctttgag gtctatattt tttaactaca atcgacctta aatttaattg
      541 tttcattagc ttttggacat gtttaagaga tactgaaatc ccttctttta ataaaacttg
      601 atggatcttt gtggcaccat agcgttgttt attcgtaaaa aaaattgttg aaatcttctt
      661 tttcaactta ttatttctga tttctgtagc tgtcaacggt ttattcttat aaaaataata
      721 gacacttcta gggatttcta ataaccgaca caacaaagaa acagtgtaat ccttacacca
      781 tttttcaata aattgaacaa gcgaatttag gttttccgag agaatatggc tgcggctttt
      841 tttaagattt caagttcctc atttaaacga gcattttctt ttctcagttc agctacttct
      901 tttccagtca gtcctgtgga ctgatcaggt aaatataaat tcttccattt gtaaatcgtt
      961 gcttcagata agccatattc tttcgctaac cctcgaacag aacgtccagt ttgattcagt
     1021 tcaacaatca tttgtttaaa attttcttca tatcgggtca taaaaattca tcctctcgta
     1081 gataagttta atttattctt gtctctacga aaagctgtct aagatttgat actaacacca
     1141 aatgttgtat acccccaaac aatacacata ttttgaagga ttaggattaa taaagctaaa
     1201 aatatagtaa gtatactaaa tatatttact atatctagta tacttactat attttaatct
     1261 tttgatcgtc tattatatat aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc
     1321 attgcaagtt tgactatttc atacatagga atatcttttg cataactaag atcccgaatg
     1381 gtttttagta cgggaggatc tacttgaact ggttttcttt ctttcgcagt gtactttcgt
     1441 tcccctaact cgacattaat atcatcttta attgaaatat cttcagcacc aattttcttc
     1501 ttatctttgt tcgttggtaa taatgccatt tagtttaatc tcctttccaa gaggtaggat
     1561 atgattggcc attgagtcag catatttagg tgtataatca aatccttcaa tatcaccatg
     1621 ttgttcatag tattcaattc tttcttctaa ttctgtaaag atgtctgcaa aaatagccca
     1681 ccatcttcga tctacataat catttaattg gataccattc tcaccataca tttctaaccg
     1741 atccgagcct ttaacaatgc ttttaaaaat gtttgattca ccatacttat caattgtttt
     1801 ttgatagttt gcgtgttgag aggaacgtcg cactgagaaa agaacaggca aaattccaat
     1861 gatttgaaga tgagactctt caaaattcgt gacaatcgga actaaaactt tattaataaa
     1921 atcttctgtt ccttccattg cgtaccgttt taattcttgc attgcaataa tataatctgc
     1981 agcagttaag aatgcacgaa ccacattatc tgttgatggt gggcagtcga taaagatata
     2041 atcgaaattt tttctcaatt cttctaaagg tttaatgaag gccaatgatc tcttacgacg
     2101 atcaggatat ttttcggaca agtattcatt aagttcttca gatccaggag agccagccat
     2161 aatatataaa tgtggactta cttgagtaat acctgaaacc cagtcccctt ttttgattgc
     2221 tgcagacatg gatcgatcaa agctagtaat ttgatacgtt gatccaatgt tggaagtagt
     2281 atttctttgt tggtcataat caattagcaa cacgtctttt tcaaaaagtt gactagaaac
     2341 aatcgctaat aaatcattgt ctgtagtctt accaatgccg ccttttctac ctgtaagcat
     2401 tacaactttt cctagtttat ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc
     2461 ctccaatttc ctatactaaa tatatttagt atacttcata tacttaatat attcgatata
     2521 tctaatatac tccaaattga taagaattca gactttataa ggaatatagt ggatatacta
     2581 aatatatttg ctatattcag tatatcagat atattcaaaa tgtcaaaata tatttgtgcg
     2641 ttttgcgttg acaatttaga gtgaaaagag gtaaacttaa ttctatagaa accacagata
     2701 attcactatt tacaaagtag caaaagcagt ggtactcttt aaacagaaaa attacataaa
     2761 aaaaagagac ggaaataatt gattttagca gaatcaatta tttctgaatc acatggttag
     2821 gacatgtgtt cacgctcgtc tcaagaatat gtaagtagct accaactact tatattaaaa
     2881 gttgatcgga taccttttcc gattgccttt attctaccaa aaaaaggcga gcaactcaac
     2941 agaagaacgt ttatttagtg tacttaaatt aaatattctt aaacactgaa gagagaacac
     3001 atactatggc tgagattaaa gctaaactgt gttctctttt tgtttgagaa aggacgttac
     3061 aatcgatgtt gagcgaaatt tattcagcta ttcttaaaaa cggcttacgt acatacaaat
     3121 atcgaaacag caccatgaaa cctgcaggtc atattgattc cagtaggagt ggtgctatct
     3181 ttgcgtaccg tagcaagcaa ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag
     3241 aagctatttt agagaacgaa aagaaactaa cgcattggac acctaatacc tatcgctttg
     3301 gtacttatgc ggatgattat cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc
     3361 aaatcaacac atttgtagtt gatatcgatt caaaagagaa ccatcaaggc gaaattattt
     3421 tagcctgttt agatcaagtg ggctacatgc caacgctaat tttagaatca gatcatggtt
     3481 atcaagttta ttttgtacta aagacaccag gctatgtcac aaagaaaagc aatttcaagg
     3541 tgatcgatgt agcaaaaaaa atttcaaaaa caattcgtct acagttggct gaaaaattgt
     3601 ccggagtgga tctaggatgt aaccacttcg gaattgctcg attcccacaa gaaaataata
     3661 ttgtttttaa agaaatgaac tatcagtatt cttttgctga ctggattcaa tggtcgatga
     3721 agcttgaatc agatcaaaaa acaaaagaag atcgagaacg aaaactaatt gtctttcctg
     3781 agaaaaaaga aattcgtcaa gtagatgaac cgtggttcga tatgttactt cataaagcaa
     3841 acatcgtcgg cggagaagga cgtcttggtc gaaataatgt gattttcacg ctttctttag
     3901 cttattactc ttcaggttat gcattggata cttgcgaata caatatgttc acattcaacg
     3961 aacggttaca ggaaccgcta tctgaagcag aattagtaaa gattgtacgt agtgcttatt
     4021 ctggtaatta tcaagcagca agcagagaat tcattattga attgtgccag caatgggttt
     4081 caccaacctt aaaaaataat gatctgttca ttcaacgacg tggctggtgg aaatttaaaa
     4141 agccacgaga tcaaagaaaa tatagtcata aaaatgaatg gaaagaagat ctattggctt
     4201 atctaagcga aaaaagtctt gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg
     4261 cttcacaact aacgatgccc aagcgaacac ttgataagat cttaaatgag ttacgagagg
     4321 agaataaagt cttctacaag gtaacacgag gaagaaatgg cggaatgatc attgcttcag
     4381 tcaaaacgtt gttcgcgagg gttattcaac taagtaaaga aaagagacta gattattcaa
     4441 caaggatcac agaggtattt ggactcaacg agagcttagt agaagagaca tttagacaat
     4501 tatcaacccc cttgaaccct cttaaagaag tgaatctttt tgaagtagat acaggttgaa
     4561 aaataaaagg tgcaaattgc cttatacaat atatctatga tacttctgta ggtgttgctc
     4621 cctttgccta gctacgtgta ggtggtatac ttataactgc gtatgcagtc ttatgtatca
     4681 tggaggaagt tgtatgtcgt taaaaaatta tgtctattca catcctgtta atgtgtttat
     4741 cattggtcgt ttgggattat ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg
     4801 gactttgtct tcttgggtta ccagggaaaa aacagttgct tctttgccga ttgagtttct
     4861 tcatgggttg tccttagctt ctggggagac tatggatcag gtatatgatt gtttgtgtgt
     4921 gttagagcag gagtatattg agtataaaat agctaacgag ggttctgttg caaagtttta
     4981 aatctactat caaataaggt agaataatag aaaaagatag caggaggaat gacgatgaat
     5041 cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg ctactatctt
     5101 cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt
     5161 tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta tcaaatttgg
     5221 aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt
     5281 aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt
     5341 tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag
     5401 cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt
     5461 aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg
     5521 aataatttga ttgaacaaga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt
     5581 ttacgcactg cctctaccac gattaaaggc atggaagcca ttcgaggatt atataagaaa
     5641 acccgaaaag aaggcactct cttcgggttt tcggtctgta ctgaaatcaa ggtattattg
     5701 ggaatcccag cttaaatcat agataccgta agggatttta ttctttattt aaaactttgc
     5761 aacagaacca actagactgt gcaaaataga gacacttgaa aaatctatcg ggggtcagaa
     5821 acttaatagt ttttgacaca taaacaaaat cgcgatataa tggggttgag agtgtttcat
     5881 aaacttatgt cataaagtta aggagatttc gcatgatttt agagaataaa ttgggattaa
     5941 ccaatcaagt tgagttggct aaggttgaag aaaaactcag taaacaaaaa gcgaaagaat
     6001 tgtacgattg tggaaaaata aatgaaatcg aagttggtac atttaaaggt ttatctgaaa
     6061 ttcatgaatt tctattttcg gatatttacg attttgccgg aaaaatccga tcggtcaata
     6121 ttgcaaaagg aaattttcga tttgcaccag ttatgtattt agaacattcg ttacagcata
     6181 ttgatcagat gccacaaact tcttttgaag agattatcaa gaaatatgtg gagatgaata
     6241 tcgctcaccc atttcgagaa ggaaatggcc gaagtactag aatttggctg gatctcattt
     6301 taaaggaaga attgcaaaaa gttgtagatt ggaacttaat cgataaagca gactatcttt
     6361 ctgcaatgga gcgaagtccc ataaacgatt tggaaatacg ctatctaatt tccaatgctc
     6421 tgactgataa aattgacgat cgcgagttat atatgaaagg aatcgatgtc agctatttct
     6481 atgaagggta ttctgagtat acgatagatg atttgtaaaa gtaggtggac caaatgacga
     6541 aatatggata tgcacgtgtc tcaaccgctt ctcaaagtac aaaagaacaa attcaacagt
     6601 tgcttgagaa ctgcgtagag aagaagaata ttttttcaga aaaatttacg ggtacaaaac
     6661 gggatcggcc aaaattcaat cagctgttgg ctggtctgaa gcctggagat gaaatagttg
     6721 ttacgaagct tgatcgattt gctcgtaaca cgagagaagc attagagatc attgaacctt
     6781 tactcgatga taacgtaacg atcaaggttt tgaatctagg aacgattgaa aatacgtcca
     6841 tggggcgaat ggtgacacgg acgttgttga gtgtagcaga aatggaacgt gatatgattg
     6901 tggaacgtac ccaagaagga aaaatgtttg caagaaagaa taatcctaat ttcagagaag
     6961 gacgtccgaa agcaacaatc acaccgaaga agaggcacgc atatgaactg cttacttcag
     7021 gtaaatcgta taaggaagtt gaagctataa ccggtttcag tcgcagtacg ctttttagaa
     7081 taaagaagaa aattgaagaa agttaaaaaa gaagtctcag cgaaccagac aggtaaatac
     7141 ttaatgcgtt ctagatatga gtctattagc atgttgtgaa atataatatt tttttattta
     7201 cttgatttct gctcaaaaaa tgtgtatggt aactgtaaaa ggtaaagtaa ttaaaggtat
     7261 gctagccacg cttactatat taggatacca tcatatattt ataatgaacg atttgttcgt
     7321 ctaccaaaca acacatatcg ttcagcgtaa gtcacgttta ggaacgtggc ttttttagtg
     7381 ttattatcga taaataatct attgactcta ttgattttat ctccatatcc ctacggtgtt
     7441 gattgtcttt agatctttca tttaagaacc aaataactat cataaaaatc agcactgctg
     7501 ctaatataat aaacagcatt cctattgtga tattactgga tttgagtcaa tatcttggac
     7561 acattttagt agagacggtt aagccatttt ttcttttgct aattcttcaa attcctgtgg
     7621 ggttagatag tgaatcgagc tatggattcg atttcggtta taaaatcctt caatgtagct
     7681 aaatagcgct cgatttgctt cttcaaaatc tgagtagcta gtcgtgtaga cttcttcttt
     7741 tttcaatgag gcatggaaag attcgattcc agcattatca taaggtgttc cttttctact
     7801 ataagaatgc cttattttgt tggttttaag ccattgttcc acttctcttg ctgtgtattg
     7861 gctgcccaag tcagtgtgta gaatcattcc ttctggaatg tgataatgta tttttgcttt
     7921 attcaatgtt tgaatgacac aatccacagt cattcgtttt gaaaatgtat aactaataat
     7981 tcttttcgtg tgtaaatcca tgattgaaga taagtaacac caaccatttt tcttagtagg
     8041 aatgtacgta atgtcagctg cccatttctc acagatcgtt tcagtagaaa agtcctgatt
     8101 taagagattc tcttttgaaa tgatggaatg gcaacagttt ttttgacaaa ttttataagg
     8161 tgcagaactt ctttccgtat gctattccga ttgtccttga caatgagcct cctcggatgt
     8221 gacgatcttc ggcaggagct aacagttaaa gttagactgc ctcgctagcg ttagcacatc
     8281 cgagctcatt atcaaggatt cgctgcgcca atctctggtt acggtaacca accggtgtct
     8341 cgtagccaag tgtaccgtgc aaccgaaggt ggttccacca attgacatag tcaaataact
     8401 ccaaatccaa ttgttgtaag gtttcaaatg tgtattgata gacaaattct actttcaacg
     8461 acttataagt tgattcagct acggcattat caaaaggaca gcctttatga ctcaatgatc
     8521 gattgatgtc aaaagttgtt aataattcat caatagcttg gttatcaaac tcttttccac
     8581 gatcagtatg aaaaatctca acctctgtca gaggtgatac ggctaaatgc ttttttacta
     8641 gaacggcatc tttatgttct ccacctccac aagaatagcc gagaatttct cgattgaaca
     8701 gatccaaaat gaaacagaca taattccatt ttttcccgac tcgtacataa gtcaagtctg
     8761 ttacgatcgc ttctaatggg ttgtctctta agaatttacg attcaatacg tttgtcgttt
     8821 tggcttcatt gcaagtagaa tgatgtactt taaaataagt atagctcgat tttaatcctc
     8881 tatcctctat ttttcatgat tctactaatt tttcgtcggc tgatctgaat gcctcgtttt
     8941 gataaggctt ttttattttt cttgagccgt aggcctttcg gctgcggata aattcttcag
     9001 cgactacttc ttc
//
