LOCUS       NZ_AP026665             9009 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Enterococcus faecium strain GK941 plasmid pGK941_4, complete
            sequence.
ACCESSION   NZ_AP026665
VERSION     NZ_AP026665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00466816
            Assembly: GCF_026073735.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 05:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,210
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 2,824
            CDSs (with protein)               :: 2,824
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 293
            CDSs (without protein)            :: 293
            Pseudo Genes (ambiguous residues) :: 1 of 293
            Pseudo Genes (frameshifted)       :: 134 of 293
            Pseudo Genes (incomplete)         :: 158 of 293
            Pseudo Genes (internal stop)      :: 80 of 293
            Pseudo Genes (multiple problems)  :: 68 of 293
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026665.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="GK941"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pGK941_4"
                     /geo_loc_name="Japan"
                     /collection_date="2015"
     gene            join(8762..9009,1..321)
                     /locus_tag="OQH75_RS15950"
                     /pseudo=""
     CDS             join(8762..9009,1..321)
                     /locus_tag="OQH75_RS15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(794..1366)
                     /locus_tag="OQH75_RS15955"
                     /old_locus_tag="EfmGK941_31790"
     CDS             complement(794..1366)
                     /locus_tag="OQH75_RS15955"
                     /old_locus_tag="EfmGK941_31790"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_073120187.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREV
                     EQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGF
                     YNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="IS3 family transposase"
     gene            complement(1467..2629)
                     /locus_tag="OQH75_RS15960"
                     /old_locus_tag="EfmGK941_31800"
     CDS             complement(join(1467..2353,2353..2629))
                     /locus_tag="OQH75_RS15960"
                     /old_locus_tag="EfmGK941_31800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_086953888.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            complement(3035..3289)
                     /locus_tag="OQH75_RS15965"
                     /old_locus_tag="EfmGK941_31830"
     CDS             complement(3035..3289)
                     /locus_tag="OQH75_RS15965"
                     /old_locus_tag="EfmGK941_31830"
                     /inference="COORDINATES: protein motif:HMM:NF013677.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(3482..3757)
                     /gene="prgO"
                     /locus_tag="OQH75_RS15970"
                     /old_locus_tag="EfmGK941_31840"
     CDS             complement(3482..3757)
                     /gene="prgO"
                     /locus_tag="OQH75_RS15970"
                     /old_locus_tag="EfmGK941_31840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(3729..4682)
                     /locus_tag="OQH75_RS15975"
                     /old_locus_tag="EfmGK941_31850"
     CDS             complement(3729..4682)
                     /locus_tag="OQH75_RS15975"
                     /old_locus_tag="EfmGK941_31850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            5294..6787
                     /locus_tag="OQH75_RS15980"
                     /old_locus_tag="EfmGK941_31860"
     CDS             5294..6787
                     /locus_tag="OQH75_RS15980"
                     /old_locus_tag="EfmGK941_31860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            6922..7209
                     /locus_tag="OQH75_RS15985"
                     /old_locus_tag="EfmGK941_31870"
     CDS             6922..7209
                     /locus_tag="OQH75_RS15985"
                     /old_locus_tag="EfmGK941_31870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="WP_265145944.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANEGSVAKF"
                     /name="type III secretion system protein PrgN"
     gene            7263..7943
                     /locus_tag="OQH75_RS15990"
                     /old_locus_tag="EfmGK941_31880"
     CDS             7263..7943
                     /locus_tag="OQH75_RS15990"
                     /old_locus_tag="EfmGK941_31880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            8141..8746
                     /locus_tag="OQH75_RS15995"
                     /old_locus_tag="EfmGK941_31890"
     CDS             8141..8746
                     /locus_tag="OQH75_RS15995"
                     /old_locus_tag="EfmGK941_31890"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     Typing          5295..6787
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac gtccatgggg
       61 cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat gattgtggaa
      121 cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag agaaggacgt
      181 ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac ttcaggtaaa
      241 tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt tagaataaag
      301 aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta aatacttaat
      361 gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt atttacttga
      421 tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa ggtatgctag
      481 ccacgcttac tatattagga taccatcata tatttataat gaacgatttg ttcgtctacc
      541 aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt tagtgttatt
      601 atcgataaat aatctattga ctctattgat tttatctcca tatccctacg gtgttgattg
      661 tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac tgctgctaat
      721 ataataaaca gcattcctat tgtgatatta ctggatttga gtcaatatct tggacacatt
      781 ttagtagaga cggttaagcc attttttctt ttgctaattc ttcaaattcc tgtggggtta
      841 gatagtgaat cgagctatgg attcgatttc ggttataaaa tccttcaatg tagctaaata
      901 gcgctcgatt tgcttcttca aaatctgagt agctagtcgt gtagacttct tcttttttca
      961 atgaggcatg gaaagattcg attccagcat tatcataagg tgttcctttt ctactataag
     1021 aatgccttat tttgttggtt ttaagccatt gttccacttc tcttgctgtg tattggctgc
     1081 ccaagtcagt gtgtagaatc attccttctg gaatgtgata atgtattttt gctttattca
     1141 atgtttgaat gacacaatcc acagtcattc gttttgaaaa tgtataacta ataattcttt
     1201 tcgtgtgtaa atccatgatt gaagataagt aacaccaacc atttttctta gtaggaatgt
     1261 acgtaatgtc agctgcccat ttctcacaga tcgtttcagt agaaaagtcc tgatttaaga
     1321 gattctcttt tgaaatgatg gaatggcaac agtttttttg acaaatttta taaggtgcag
     1381 aacttctttc cgtatgctat tccgattgtc cttgacaatg agcctcctcg gatgtgacga
     1441 tcttcggcag gagctaacag ttaaagttag actgcctcgc tagcgttagc acatccgagc
     1501 tcattatcaa ggattcgctg cgccaatctc tggttacggt aaccaaccgg tgtctcgtag
     1561 ccaagtgtac cgtgcaaccg aaggtggttc caccaattga catagtcaaa taactccaaa
     1621 tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa attctacttt caacgactta
     1681 taagttgatt cagctacggc attatcaaaa ggacagcctt tatgactcaa tgatcgattg
     1741 atgtcaaaag ttgttaataa ttcatcaata gcttggttat caaactcttt tccacgatca
     1801 gtatgaaaaa tctcaacctc tgtcagaggt tgtttgatac ggctaaatgc tttttttact
     1861 agaacggcat ctttatgttc tccacaagaa tagccgagaa tttctcgatt gaacagatcc
     1921 aaaatgaaac agacataatt ccattttttc ccgactcgta cataagtcaa gtctgttacg
     1981 atcgcttcta atgggttgtc tcttaagaat ttacgattca atacgtttgt cgttttggct
     2041 tcattgcaag tagaatgatg tactttaaaa taagcaacag tatagctcga ttttaatcct
     2101 ctatttttca tgattctact aatttttcgt cggctgatct gaatgcctcg ttttgataag
     2161 gcttttttta tttttcttga gccgtaggcc tttcggctgc ggataaattc ttcagcgact
     2221 acttcttcaa gttctgattc gtctttcttt ggttttgatt gataataata ggactgacgt
     2281 gatagaccta atattctgca catcgctgat atagggtaaa gatgcttatt cgcatcgatt
     2341 acttgtctct tcgtccgaat atcagcgctg cttgctttaa aatatcattt tccatttcta
     2401 attgctggtt tcttttacgt agttctaaca attctttttg ttcaggcgta agattatctt
     2461 tttctttgaa tgaaccactc gttttaaatt gctttaccca tttgtcaaat gctgaagccg
     2521 ttagttcata ttctcgaatg atttctacac gtggctttcc agctaagtaa agattgacga
     2581 tttgttgctt gaattcttgt gaaaaagttc ttcgtgttct cttagacata aaaattcctc
     2641 ctggtatgtt ttcttctagt ctacacacct taatttttct gtctagttaa ttgtagccta
     2701 tccatgatcg gtttattaga cctttgaggt ctatattttt taactacaat cgaccttaaa
     2761 tttaattgtt tcattagctt ttggacatgt ttaagagata ctgaaatccc ttcttttaat
     2821 aaaacttgat ggatctttgt ggcaccatag cgttgtttat tcgtaaaaaa aattgttgaa
     2881 atcttctttt tcaacttatt atttctgatt tctgtagctg tcaacggttt attcttataa
     2941 aaataataga cacttctagg gatttctaat aaccgacaca acaaagaaac agtgtaatcc
     3001 ttacaccatt tttcaataaa ttgaacaagc gaatttaggt tttccgagag aatatggctg
     3061 cggctttttt taagatttca agttcctcat ttaaacgagc attttctttt ctcagttcag
     3121 ctacttcttt tccagtcagt cctgtggact gatcaggtaa atataaattc ttccatttgt
     3181 aaatcgttgc ttcagataag ccatattctt tcgctaaccc tcgaacagaa cgtccagttt
     3241 gattcagttc aacaatcatt tgtttaaaat tttcttcata tcgggtcata aaaattcatc
     3301 ctctcgtaga taagtttaat ttattcttgt ctctacgaaa agctgtctaa gatttgatac
     3361 taacaccaaa tgttgtatac ccccaaacaa tacacatatt ttgaaggatt aggattaata
     3421 aagctaaaaa tatagtaagt atactaaata tatttactat atctagtata cttactatat
     3481 tttaatcttt tgatcgtcta ttatatataa tccgttcctc gtcgtttaaa gtatcaacat
     3541 aggcgtccat tgcaagtttg actatttcat acataggaat atcttttgca taactaagat
     3601 cccgaatggt ttttagtacg ggaggatcta cttgaactgg ttttctttct ttcgcagtgt
     3661 actttcgttc ccctaactcg acattaatat catctttaat tgaaatatct tcagcaccaa
     3721 ttttcttctt atctttgttc gttggtaata atgccattta gtttaatctc ctttccaaga
     3781 ggtaggatat gattggccat tgagtcagca tatttaggtg tataatcaaa tccttcaata
     3841 tcaccatgtt gttcatagta ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa
     3901 atagcccacc atcttcgatc tacataatca tttaattgga taccattctc accatacatt
     3961 tctaaccgat ccgagccttt aacaatgctt ttaaaaatgt ttgattcacc atacttatca
     4021 attgtttttt gatagtttgc gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa
     4081 attccaatga tttgaagatg agactcttca aaattcgtga caatcggaac taaaacttta
     4141 ttaataaaat cttctgttcc ttccattgcg taccgtttta attcttgcat tgcaataata
     4201 taatctgcag cagttaagaa tgcacgaacc acattatctg ttgatggtgg gcagtcgata
     4261 aagatataat cgaaattttt tctcaattct tctaaaggtt taatgaaggc caatgatctc
     4321 ttacgacgat caggatattt ttcggacaag tattcattaa gttcttcaga tccaggagag
     4381 ccagccataa tatataaatg tggacttact tgagtaatac ctgaaaccca gtcccctttt
     4441 ttgattgctg cagacatgga tcgatcaaag ctagtaattt gatacgttga tccaatgttg
     4501 gaagtagtat ttctttgttg gtcataatca attagcaaca cgtctttttc aaaaagttga
     4561 ctagaaacaa tcgctaataa atcattgtct gtagtcttac caatgccgcc ttttctacct
     4621 gtaagcatta caacttttcc tagtttatct gatgttttaa atgcagtccg taagttttcc
     4681 atagttccct ccaatttcct atactaaata tatttagtat acttcatata cttaatatat
     4741 tcgatatatc taatatactc caaattgata agaattcaga ctttataagg aatatagtgg
     4801 atatactaaa tatatttgct atattcagta tatcagatat attcaaaatg tcaaaatata
     4861 tttgtgcgtt ttgcgttgac aatttagagt gaaaagaggt aaacttaatt ctatagaaac
     4921 cacagataat tcactattta caaagtagca aaagcagtgg tactctttaa acagaaaaat
     4981 tacataaaaa aaagagacgg aaataattga ttttagcaga atcaattatt tctgaatcac
     5041 atggttagga catgtgttca cgctcgtctc aagaatatgt aagtagctac caactactta
     5101 tattaaaagt tgatcggata ccttttccga ttgcctttat tctaccaaaa aaaggcgagc
     5161 aactcaacag aagaacgttt atttagtgta cttaaattaa atattcttaa acactgaaga
     5221 gagaacacat actatggctg agattaaagc taaactgtgt tctctttttg tttgagaaag
     5281 gacgttacaa tcgatgttga gcgaaattta ttcagctatt cttaaaaacg gcttacgtac
     5341 atacaaatat cgaaacagca ccatgaaacc tgcaggtcat attgattcca gtaggagtgg
     5401 tgctatcttt gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta tggtcattac
     5461 ttcggaagaa gctattttag agaacgaaaa gaaactaacg cattggacac ctaataccta
     5521 tcgctttggt acttatgcgg atgattatcg aacagtggtt aaaggtcatt ctgagaaaaa
     5581 cttaagtcaa atcaacacat ttgtagttga tatcgattca aaagagaacc atcaaggcga
     5641 aattatttta gcctgtttag atcaagtggg ctacatgcca acgctaattt tagaatcaga
     5701 tcatggttat caagtttatt ttgtactaaa gacaccaggc tatgtcacaa agaaaagcaa
     5761 tttcaaggtg atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac agttggctga
     5821 aaaattgtcc ggagtggatc taggatgtaa ccacttcgga attgctcgat tcccacaaga
     5881 aaataatatt gtttttaaag aaatgaacta tcagtattct tttgctgact ggattcaatg
     5941 gtcgatgaag cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa aactaattgt
     6001 ctttcctgag aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata tgttacttca
     6061 taaagcaaac atcgtcggcg gagaaggacg tcttggtcga aataatgtga ttttcacgct
     6121 ttctttagct tattactctt caggttatgc attggatact tgcgaataca atatgttcac
     6181 attcaacgaa cggttacagg aaccgctatc tgaagcagaa ttagtaaaga ttgtacgtag
     6241 tgcttattct ggtaattatc aagcagcaag cagagaattc attattgaat tgtgccagca
     6301 atgggtttca ccaaccttaa aaaataatga tctgttcatt caacgacgtg gctggtggaa
     6361 atttaaaaag ccacgagatc aaagaaaata tagtcataaa aatgaatgga aagaagatct
     6421 attggcttat ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc tgtcaaaaaa
     6481 agaattggct tcacaactaa cgatgcccaa gcgaacactt gataagatct taaatgagtt
     6541 acgagaggag aataaagtct tctacaaggt aacacgagga agaaatggcg gaatgatcat
     6601 tgcttcagtc aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa agagactaga
     6661 ttattcaaca aggatcacag aggtatttgg actcaacgag agcttagtag aagagacatt
     6721 tagacaatta tcaaccccct tgaaccctct taaagaagtg aatctttttg aagtagatac
     6781 aggttgaaaa ataaaaggtg caaattgcct tatacaatat atctatgata cttctgtagg
     6841 tgttgctccc tttgcctagc tacgtgtagg tggtatactt ataactgcgt atgcagtctt
     6901 atgtatcatg gaggaagttg tatgtcgtta aaaaattatg tctattcaca tcctgttaat
     6961 gtgtttatca ttggtcgttt gggattatct gtggaagtgt tctgtgagct ttatggtttt
     7021 aagcagggga ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc tttgccgatt
     7081 gagtttcttc atgggttgtc cttagcttct ggggagacta tggatcaggt atatgattgt
     7141 ttgtgtgtgt tagagcagga gtatattgag tataaaatag ctaacgaggg ttctgttgca
     7201 aagttttaaa tctactatca aataaggtag aataatagaa aaagatagca ggaggaatga
     7261 cgatgaatca ttttaaagga aagcaatttc agcaggatgt gattattgta gccgtgggct
     7321 actatcttcg ttataacctt agctatcgtg aagttcaaga aatcttatat gatcgtggca
     7381 ttaacgtttc tcatacgacg atttatcgtt gggtgcaaga atatggcaaa ctactctatc
     7441 aaatttggaa aaagaaaaat aaaaaatcct tttattcatg gaaaatggat gaaacgtaca
     7501 tcaaaattaa aggaaaatgg cattatttgt atcgagccat cgatgcagat ggtttaacct
     7561 tggatatttg gttacgtaaa aaacgggaca cacaagcagc ctatgctttt cttaagcggt
     7621 tagtgaagca gtttgatgaa ccgaaggttg tagtcacaga taaagccccc tctattacaa
     7681 gtgcctttaa gaaactaaaa gaatacggct tttatcaagg gacagaacat cgtaccatta
     7741 aatacctgaa taatttgatt gaacaagacc atcgtccagt aaagagacgc aataaattct
     7801 atcgaagttt acgcactgcc tctaccacga ttaaaggcat ggaagccatt cgaggattat
     7861 ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact gaaatcaagg
     7921 tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa
     7981 aactttgcaa cagaaccaac tagactgtgc aaaatagaga cacttgaaaa atctatcggg
     8041 ggtcagaaac ttaatagttt ttgacacata aacaaaatcg cgatataatg gggttgagag
     8101 tgtttcataa acttatgtca taaagttaag gagatttcgc atgattttag agaataaatt
     8161 gggattaacc aatcaagttg agttggctaa ggttgaagaa aaactcagta aacaaaaagc
     8221 gaaagaattg tacgattgtg gaaaaataaa tgaaatcgaa gttggtacat ttaaaggttt
     8281 atctgaaatt catgaatttc tattttcgga tatttacgat tttgccggaa aaatccgatc
     8341 ggtcaatatt gcaaaaggaa attttcgatt tgcaccagtt atgtatttag aacattcgtt
     8401 acagcatatt gatcagatgc cacaaacttc ttttgaagag attatcaaga aatatgtgga
     8461 gatgaatatc gctcacccat ttcgagaagg aaatggccga agtactagaa tttggctgga
     8521 tctcatttta aaggaagaat tgcaaaaagt tgtagattgg aacttaatcg ataaagcaga
     8581 ctatctttct gcaatggagc gaagtcccat aaacgatttg gaaatacgct atctaatttc
     8641 caatgctctg actgataaaa ttgacgatcg cgagttatat atgaaaggaa tcgatgtcag
     8701 ctatttctat gaagggtatt ctgagtatac gatagatgat ttgtaaaagt aggtggacca
     8761 aatgacgaaa tatggatatg cacgtgtctc aaccgcttct caaagtacaa aagaacaaat
     8821 tcaacagttg cttgagaact gcgtagagaa gaagaatatt ttttcagaaa aatttacggg
     8881 tacaaaacgg gatcggccaa aattcaatca gctgttggct ggtctgaagc ctggagatga
     8941 aatagttgtt acgaagcttg atcgatttgc tcgtaacacg agagaagcat tagagatgaa
     9001 cctttactc
//
