LOCUS       NZ_AP026664             9527 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Enterococcus faecium strain GK941 plasmid pGK941_3, complete
            sequence.
ACCESSION   NZ_AP026664
VERSION     NZ_AP026664.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00466816
            Assembly: GCF_026073735.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 05:59:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,210
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 2,824
            CDSs (with protein)               :: 2,824
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 293
            CDSs (without protein)            :: 293
            Pseudo Genes (ambiguous residues) :: 1 of 293
            Pseudo Genes (frameshifted)       :: 134 of 293
            Pseudo Genes (incomplete)         :: 158 of 293
            Pseudo Genes (internal stop)      :: 80 of 293
            Pseudo Genes (multiple problems)  :: 68 of 293
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026664.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="GK941"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pGK941_3"
                     /geo_loc_name="Japan"
                     /collection_date="2015"
     gene            complement(join(9442..9527,1..277))
                     /locus_tag="OQH75_RS15885"
     CDS             complement(join(9442..9527,1..277))
                     /locus_tag="OQH75_RS15885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_265145941.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTYPHKEHIHNRT
                     QIKSISTITLSLIRSI"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(259..639)
                     /locus_tag="OQH75_RS15890"
                     /old_locus_tag="EfmGK941_31660"
     CDS             complement(259..639)
                     /locus_tag="OQH75_RS15890"
                     /old_locus_tag="EfmGK941_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002292464.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1082..1219)
                     /locus_tag="OQH75_RS15895"
                     /old_locus_tag="EfmGK941_31680"
     CDS             complement(1082..1219)
                     /locus_tag="OQH75_RS15895"
                     /old_locus_tag="EfmGK941_31680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(1333..1935)
                     /locus_tag="OQH75_RS15900"
                     /old_locus_tag="EfmGK941_31690"
     CDS             complement(1333..1935)
                     /locus_tag="OQH75_RS15900"
                     /old_locus_tag="EfmGK941_31690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_265145942.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDNHISYLFQNYALLENQTVQYNFELAKKFNSNISN
                     DHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGNLDKENS
                     TFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(2641..4065)
                     /locus_tag="OQH75_RS15905"
                     /old_locus_tag="EfmGK941_31710"
     CDS             complement(2641..4065)
                     /locus_tag="OQH75_RS15905"
                     /old_locus_tag="EfmGK941_31710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265145943.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSNHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYGT
                     PTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTIK
                     DSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIYH
                     LSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTTN
                     LYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFIG
                     QKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYK
                     EYAKMIEKLFRRHTII"
                     /name="hypothetical protein"
     gene            complement(4119..4493)
                     /locus_tag="OQH75_RS15910"
                     /old_locus_tag="EfmGK941_31720"
     CDS             complement(4119..4493)
                     /locus_tag="OQH75_RS15910"
                     /old_locus_tag="EfmGK941_31720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            4626..5087
                     /locus_tag="OQH75_RS15915"
                     /old_locus_tag="EfmGK941_31730"
     CDS             4626..5087
                     /locus_tag="OQH75_RS15915"
                     /old_locus_tag="EfmGK941_31730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5401..5571)
                     /locus_tag="OQH75_RS15920"
                     /old_locus_tag="EfmGK941_31740"
     CDS             complement(5401..5571)
                     /locus_tag="OQH75_RS15920"
                     /old_locus_tag="EfmGK941_31740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5587..6123)
                     /locus_tag="OQH75_RS15925"
                     /old_locus_tag="EfmGK941_31750"
     CDS             complement(5587..6123)
                     /locus_tag="OQH75_RS15925"
                     /old_locus_tag="EfmGK941_31750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002303560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(6144..7088)
                     /locus_tag="OQH75_RS15930"
                     /old_locus_tag="EfmGK941_31760"
     CDS             complement(6144..7088)
                     /locus_tag="OQH75_RS15930"
                     /old_locus_tag="EfmGK941_31760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            7725..7811
                     /locus_tag="OQH75_RS15935"
     CDS             7725..7811
                     /locus_tag="OQH75_RS15935"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(7932..8621)
                     /locus_tag="OQH75_RS15940"
                     /old_locus_tag="EfmGK941_31770"
     CDS             complement(7932..8621)
                     /locus_tag="OQH75_RS15940"
                     /old_locus_tag="EfmGK941_31770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303565.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(8623..8901)
                     /locus_tag="OQH75_RS16170"
     CDS             complement(8623..8901)
                     /locus_tag="OQH75_RS16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(8861..9313)
                     /locus_tag="OQH75_RS15945"
     CDS             complement(8861..9313)
                     /locus_tag="OQH75_RS15945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_265145940.1"
                     /translation="MAEIKIKEKNEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFG
                     IHCFNRGKNFTYELIHKKKKVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARA
                     SIRETAEEVRREQRTLTPEQPEIRPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     BGC             1..9493
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6145..7088)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..277)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.913"
                     /coverage="100.0"
                     /evalue="3.45e-52"
     Typing          complement(8865..9499)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.113"
                     /coverage="100.0"
                     /evalue="2.93e-137"
ORIGIN
        1 tgtaaatcgc gatttggtat tctggcgcga ttttttcagc gtattctttc cctagttggt
       61 tggctttttc agcggtaatt tctcctggtc gaaaggactg aatcaataaa tgtgcttgta
      121 cacgatcgtc ttttccataa agcatgcgta tttgtttcat ttcacttttg gcataatcaa
      181 tgtcacagtt tattcctgtt ttgacggttg ctcgtggttc tgcgtaattg attgctcggc
      241 tacatgaagt ggttcgactt actttaactg ttgccatatc ttttgtagct ccttttgcat
      301 gatttttagg ttttcggata aacgttgagc gtcaccagga gcaacgtcat gttggtgtgt
      361 attgcaccac ttagcaattt gattgatatt ccctccagct ttggctaaat cgcgcgcgat
      421 tgcctgggca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc
      481 tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca gctttgcaaa
      541 ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg tatatttttt
      601 agaaggatcg ctagccaaat tttgattttg ttcgctcata agtcccaccc tttcagtacg
      661 tcctacggac gtatcgttcg caataccatt atacctaaaa aggtacaatg agtaaagctc
      721 actaaaaggg tttggcaagc tctatatttg ctagccaaat acctccgtat ttggtgcaaa
      781 taaccgcttg tcaaaggggc gcaagctccc cctttgaacc cccctcaaaa cccacgctcc
      841 acaatacaaa aaaagctagc aaatgctagc cactttttgt aaagtttcgc ttatttcacc
      901 gaagatgaaa ttgttgcttt gcaacaaaaa acataaatgc ttttttgtgt aaactactaa
      961 ctatttagct aaaactgata gttttattaa acattcagca atatttttag ccaatttttt
     1021 atcagacttt cggcgagtca ttatgataat atgtccactt tttgttactt ctaccaaaaa
     1081 tctagcaata actctcccat tcttcagttg atttagtttc ataagtcttt tgtaattgac
     1141 ggtttaattt tttgggcaat tgcaaagaat aaataagtct atcataaaat ttttcatact
     1201 cttttttcaa taaaatcact tgggtgcctc ctttttatta agtgattatt taaattttgg
     1261 gataggagag atggaatata aaataaatac ccgtggtgct agttttaaat cagctagcat
     1321 cacgggtaaa aattataatt ctataatatt agttccatag tttagaacat ttaagtcatg
     1381 tgttactagt acaacggctt ttttttcttt ttctgttaaa tttttcagaa agttcattat
     1441 aaaagttgag ttttctttat ctaaatttcc tgtaggttca tctgcaaata atatatcaaa
     1501 tggttttaac atatttctaa ttaatgctat tctttgttgt tctccaccgc ttaagagaaa
     1561 gattttttct tttaagatat cttttggcaa agaaaaatct gttaataaat tataaatatg
     1621 atcattggaa atattactgt taaacttttt tgctaattcg aaattgtatt gtactgtttg
     1681 attttcaaga agagcatagt tttgaaataa atagctaata tgattatcat gtttagaaac
     1741 tttcttttca aaatagaata agttaccggt atattgttta tctattaggc ttaaattatt
     1801 taacagagta gtttttccac taccactttt tcctgttact ataataaaat ctttttctga
     1861 tattgaaaag ttccaatttt gtttgatctt ttttttatta attgacaggg tgaaattctc
     1921 aactgataat atcataaaat attctcctta aaatatttaa aactattttt ttataaataa
     1981 taatagaagt aattagattt attaaaaaga tcataaaaat tataaaatac aagtaagtgt
     2041 ttttgaaaat atcaattgaa aacacatagt agcaaataac tctattgtaa aagggagtaa
     2101 acataaatct aaaacttgac ttattggtga atatccaaat atttttttaa agctattttt
     2161 ttgaatttat agtaaaacta acaaaaatca tttgaaaatt aattattata aataatccta
     2221 atattaaaag atattgtaga tatgcattta atttatttac ttttttgaga taataattca
     2281 ccagtttctt gaaaaacaga ataagaaaaa tttatgatat tttctaggtt aaattctttt
     2341 atatacggat taatttgatt aaaaagtttt ggtttgaagt atgtttctga tcaaaaaata
     2401 atccaccatc actagtcaat atatttcccc aaaataatga ttctgttatt tctggattaa
     2461 taactctaac tatgggatca gttattgtat agttatcttt tgcctttcca tataaggtat
     2521 taaaactaaa atacttttgg ttattattaa caaatattag ttttattttt aaatttttca
     2581 aattaggagt tttataagga gcttgaggag gagagtcttc aagtctaaac tgtatgtctt
     2641 ctaaataatt gtatgtcttc taaataattt ttcaatcatt tttgcatatt ctttatattt
     2701 tttaggtact agaatgtaag tagttagtgg atcagagttt attaaattta agtcagtaga
     2761 ttcttcaaaa gaaataggat tgtaatattt tagatagttt aaatcaacac atattcctgc
     2821 accagcaggt tgactaacat agtcttcaaa atttggataa actttttgtc caataaaaaa
     2881 ttctttatta ttttcttgga tagttgcgta attgtatggc gcgataataa aagttttata
     2941 attcttctgt attttataaa caaatttact agcttttttc aagtagttat tttcttctac
     3001 ggtgttgttt ctatttaatt gattagtaat atttgtttta tataaattag tagttctttc
     3061 ccaatattta agagaattat tttcggcttc attatggata tttattactt ttgtagaagc
     3121 tattaacaga aggatcatgc ttaagaatag acagatattt aatatacaaa gatttaactt
     3181 aagtggtcgt gaatttttta gataattttt tatactagat aaatgataga tacttagagt
     3241 aactatgata aataaaaaac taattataaa atgaaaaata ctacatagaa gaaatgatag
     3301 gaaaaaattt tcgttactgt aatatatgct caaaaaaata gataaaatga aagttataat
     3361 caaagaacaa aataagaggt ttaactcttt aatagaatct tttatagtat caaaagagtt
     3421 atagcctaac atttttttta gagtgatttc tttcattttt gaaaaagcaa atataattgt
     3481 agtaattaca ataaatatac taagatagca gataacaaga acaatattag tattaatttg
     3541 aaaagtatct gattgattga tattggaaat agtaggtgtt ccatattttt tcagtacagg
     3601 aattaatttg tttgagtttc cttgtacata taatatttca cttaatccaa tattttccac
     3661 atgagaaaaa ggataaatat ttatatttaa aaaattattt ggaagtttta attggtactt
     3721 agaatttttg ttattagtat aatggttaga atcgtttctt gctaatatat tttttttaga
     3781 aaatttccag tgtttgttag cgttagggtt tgtagcaaga atagttagtt ggttaacagc
     3841 agtaaaacta tattgagaaa tattaatatt ttcttgtgta gatatttcat acaaagcgtt
     3901 tatataactg ttaaagtcca tatcatcttt atattgcata tgaatttcag cggtattttt
     3961 atatagcgct tcagaagtct ttataccatt tataacatta gtagaaaaga atagtattgt
     4021 aatgcagaca aaaagtgata aaacagcgtt tatttttttt aacatatttt gaatttcctt
     4081 tcaaatgtaa ataaaaaaga gagtttaaac tctctttttt aataaacatt ccaccaacca
     4141 gtatttccag taagtgtggc attgcatgtt gcataagctc ttactccagc tttctgaact
     4201 gatcgtactt gaccgcctcg tccattttta gctgttgcac tatgtttagc tctagcatgg
     4261 tagtaatttg cataaacttt aaatccatta accccacagt tgaaatctcc gccgccagca
     4321 gcatatctca atttactaga agaaaatcct tcttctgtag cttccattcc tcctccgtaa
     4381 atggcggtat ggtcttcggg tacatctaga tttaaagctt ctgtagcact aactattcct
     4441 ccactagtta ataagacacc cattaaagca gaaagacata aaaatttttt cataacttat
     4501 ttctcctatc ttttttacta gaattatttt attttagtgt gttttataaa ataaagaact
     4561 tttttggcgc gttagtattt ttatgataaa attttatgta aataatcgtt tacataggag
     4621 aaaaaatgga ggatttagga tctaagctaa gagaattaag agtgaaaaaa gggtatactc
     4681 aagaatatgt ggcaagaaaa tttaatgtta cacgtcaaac tatttcaaag tgggaaaata
     4741 acaaaacaac accagatata gagacactaa aaaaaatttg tgaattttat agtactaaca
     4801 taaattctat tttaaatgaa gataatttaa aaaataaaca aaataattta ctggtattga
     4861 cagctattat ttttaatata tttgttacta tattaggttt atttgaattg attcaaatga
     4921 atttttttag ctttacacac ggaatattta cttggtttgc tctatctgga ttaatagtaa
     4981 tagatatctt aaattatttt tatatttact attcttattt aaaaaatgaa aaattttttt
     5041 atacaatatt ttttccccta gtgattttga ttattttatt aagctaatat gaagtagtta
     5101 gttatttaat tgcatagttg acataatgta cagtatgcta agtagcgcct gtatcaatcg
     5161 atacaggggc tcactttgtg atttttaatt ggtctaggtc aaaagctctt tttaaggctt
     5221 gattttctgc agttgcttct ttccgtttct ttgtgaattt ttaccagtca ttttttactg
     5281 gattgttatt ttttataaaa tatgataaaa ttttggtagc gctttataag cgcgttgctt
     5341 tttcaagaat aaactctcat tattttttta actggctgct cgaaagagca gtctttttta
     5401 ttatttatcc gtttctttta caaacaattc ttcgttagga ctgttgtaat aatttggatt
     5461 caatttcaat tcaccatgtt tattcccttt attgatcact gacaataaca tcggactgaa
     5521 gcctagaatt cctagtaaaa tagaaccgaa tagaagattt tttcttgtca tgaatattcc
     5581 ctccagttat ttccaaagtt gccaccattt ttgcttcttc tttgtgtgtt tttctatcga
     5641 ttctgtttct tcagaaagaa tagtagttga ttcatcttct ggagtttctt ccgtataaac
     5701 taattgtaac tgttcttgca gtttttcgat ctgttgattt gcctgtaggg tgagctgttg
     5761 ttgctggtcg agtaattttt gcatattaga attattttgc tctttaaggg aaagttgttc
     5821 tcgtaaaaat tcattttctt cacgcaactt atctagcaac tcgtttacaa ccacgttttt
     5881 gcttgatata taaatgtttt tatcgttgtc tttttcatac tcatgcaacc tatctagtaa
     5941 cctttggtta cctaagttgc tagcaagttg caatactcca gaacgcaata caaccttata
     6001 accacgctca tttttagcta tcatggaaga atctaatttt tggatatgtt tcgatattgc
     6061 ttgcctactc atcttaaaaa gatctgacaa ttcctttatt gtaaaggttt cttgtggttg
     6121 catactggtt gcctcctagg ttgctactgg aataaattat ctaaatcaga taataaactt
     6181 tttttctctt tttccgaaag tgcggactct gtttgttttt caagagcttt tttctctttt
     6241 tctagttgtt tgatttgttg gaggagttca agttctcgtt tcttctgttt gattttttcg
     6301 ttaattgttg tagcgggttc ttcgtattct ttttgtttat cagctaaagc caatgcttta
     6361 ttttggtctt ctttggttgg tgcatgtttg ttaaagtcgt ctgctttttt gatctcaggt
     6421 ttgaacgtaa attgatagcc aataacaggt tttccccgtc cttttccgta tttctttttg
     6481 attgttagtc ctttgaagat tgcagttagt tcttgacgta taggttttat aactcgttta
     6541 tctatatcag gttgcttata acttttagga atatctaata attcaagaaa gtcttctttg
     6601 gagaaatatg cgaaaccttt ggttctgtat tgctttaaca gacgaaacat agtctttgca
     6661 tagccacttt gtagctcgtt gaattgttgt aaagaatagc gtacccactc gtctaaatta
     6721 tttaacaaag gcagtgcttt ttcgtaaatt cgtatatcta cgtaaggatt ttctacgtcc
     6781 ccgttgattt taaattctgt aaacattaca aacatatcgt aagaaagccc agactcactt
     6841 ctacgaccaa agcgtaaaga taagattttt tcataagttg cttttaaatc ttctacaaat
     6901 cgtacatttg cagttgcttt gtagttactt aaatctttta gttggtcaaa cgtaaaacga
     6961 actgtcttat cccctttttc acgcattcta gacacgatgg aaaaaaacaa attcatttca
     7021 accggtgtaa attttcttaa aggaattgta tttaattcat ggtggtattt tacaatctca
     7081 ttagccatat aatcacctca atccaattat gaatgacatt ttttaaatgt caagacattc
     7141 atatttgacg ttcaaacaga caaaatgtcc ttcataaaca gacaaaatgt ccttcataaa
     7201 cagacaaaat gtccttcata aacagacaaa atgtccttca taacattctg tatccattgg
     7261 tagagtaagg acacagctta cggtaaagag gtttaaaaag aggtttaaaa agagatataa
     7321 aaagaggggg gctaattttc ccgcctaaaa accacttctt ttttctttaa acaggttcgc
     7381 gcatttttca aaactgccaa tccaaaacct tgcttcctac ggtcgccaaa aaggtgtgat
     7441 cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc tcctttgcta aaaacatgtt
     7501 ggtaaagagg cctttgagcg gttgtgaatg cttatctaag ccgttttaag acagtcttgg
     7561 ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
     7621 cctaaaataa gcgatactta aaaacaccac acaagtttaa ttttttaaga tataataact
     7681 tgtcgcctat ccgacagtct tgcgactgga aggaggtgta tctaatgttt gaattgttca
     7741 tttgccccct tcttatagaa gtttgtgtct ctttattcag ttattggttg aataaccgaa
     7801 acaagaagta aaatcaataa aggcgacatc aacctgaaaa aaagccccag agaatccgac
     7861 ctctctgggg ctttcgtgta tctaatgttt gatcatttgc atgtttagtt taacatgatt
     7921 gccgatcaaa ttcaattttg ctgttgctga tctaaccatt ccttgacact ttctagataa
     7981 tttaatgccg tttctttttt cgttggactt tgtatgttag ttgcttgttg aaacaggttt
     8041 tcgtagcgtt catatgtgac agattgttgt ttttcttttt tttcttcttc actggcaaac
     8101 caagggtttg tgtctccaaa tagatcacta caggcttctt ctatctttgt atcttttaca
     8161 tattgcacgt ccttttttac ttgttcatat ttttcttggt attctttttg cggttcttct
     8221 ggcaaattcc gataaagtcg agtgagtgtt tccatatttt tatccgtcaa acgcttttct
     8281 tgcttcacaa aaatatcatt catgtcctca taacaagcgg taaatttttc tattttttgt
     8341 gctaaggctt gctctttttt ctttttatta cgtgctgatt catacttttg agtgacattt
     8401 cctaataaaa tacttctaga aacgcgctta ttgccctcta aaacgcctaa taagccgctt
     8461 gaaacgatta tcaaggaaaa tatacagcca agcaagaaaa atcgcttaaa tcgaatttta
     8521 gagcctttta atgaatatct tgtttggaaa attttataga tgagccaaat acttcctgaa
     8581 atactaacaa aaatgagtag atagggtaaa taaaatgcca ttttagcctc cccatactaa
     8641 ccaaaatgcg aacagtgtca ggactaagca acataagggg gcaagaaaat ccattgatcg
     8701 ttgaatgaac gccttttttt gttctttatt tcgctgatct aatagctctt gatggagttt
     8761 tttaacttgt aaatcaatcg tagtttgtat cctttcttgt gtgtagggag caagaaaagc
     8821 acgttgtttc tttatttcgt tttctatgtc ttttaacgtt ttagagcgat aattcttgtt
     8881 ggtggtctgt tcctctttca acgtgttgtt catggtttct tcgagccggt ctaatttccg
     8941 gttgttcagg cgtaagagtt cgttgttctc ttcgtacttc ttctgcagtt tcgcgaatgc
     9001 tagctcgagc tcttcgtagc tcgttggttc gctgttggtt ataggttcgt tctcgtctgt
     9061 ccagctcatg taaaatcgtc tccttttcgt agtcctttcc tagtctgttg gatcgtactt
     9121 ttttcttttt gtggatcaat tcgtaagtga aatttttccc gcgattgaag caatgaatgc
     9181 cgaatgtttt gagggaatca caaaattggt cataggttct ggttttcttg tttttcatgg
     9241 tttgatcaat cgcaaaccga atttcatctt tccaaacagg ttcatttttt tcttttattt
     9301 tgatttcagc catggttcgt ttttcttctt tttttcctcg ttcagtgata cttaaaccat
     9361 gagttaaaca aatttcgtcg ttgatatcgt aacacttatc taggaatgct tgaccatgtg
     9421 cttgaaactt ttttcctgtt tctaaattga ccgaattaat gacaatgtga ttgtggatat
     9481 gctctttatc tgtgtgcgat tgtggatatg ctctttatgt ggatatg
//
