LOCUS       NZ_AP026659            19291 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Enterococcus faecium strain GK923 plasmid pGK923_3, complete
            sequence.
ACCESSION   NZ_AP026659
VERSION     NZ_AP026659.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00466815
            Assembly: GCF_026073625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19291)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 05:52:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,201
            CDSs (total)                      :: 3,108
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 123 of 277
            Pseudo Genes (incomplete)         :: 149 of 277
            Pseudo Genes (internal stop)      :: 83 of 277
            Pseudo Genes (multiple problems)  :: 69 of 277
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026659.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="GK923"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pGK923_3"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(join(19228..19291,1..320))
                     /locus_tag="OQH82_RS15850"
     CDS             complement(join(19228..19291,1..320))
                     /locus_tag="OQH82_RS15850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_265169991.1"
                     /translation="MCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKI
                     KKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNP
                     LEACLFHFGSVQSRNSRLFLWRT"
                     /name="IS3 family transposase"
     gene            complement(356..646)
                     /locus_tag="OQH82_RS15855"
                     /old_locus_tag="EfmGK923_31440"
     CDS             complement(356..646)
                     /locus_tag="OQH82_RS15855"
                     /old_locus_tag="EfmGK923_31440"
                     /inference="COORDINATES: protein motif:HMM:NF013677.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000044789.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGRRDK"
                     /name="transposase"
     gene            complement(1052..1306)
                     /locus_tag="OQH82_RS15860"
                     /old_locus_tag="EfmGK923_31460"
     CDS             complement(1052..1306)
                     /locus_tag="OQH82_RS15860"
                     /old_locus_tag="EfmGK923_31460"
                     /inference="COORDINATES: protein motif:HMM:NF013677.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(1499..1774)
                     /gene="prgO"
                     /locus_tag="OQH82_RS15865"
                     /old_locus_tag="EfmGK923_31470"
     CDS             complement(1499..1774)
                     /gene="prgO"
                     /locus_tag="OQH82_RS15865"
                     /old_locus_tag="EfmGK923_31470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(1746..2699)
                     /locus_tag="OQH82_RS15870"
                     /old_locus_tag="EfmGK923_31480"
     CDS             complement(1746..2699)
                     /locus_tag="OQH82_RS15870"
                     /old_locus_tag="EfmGK923_31480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            3311..4804
                     /locus_tag="OQH82_RS15875"
                     /old_locus_tag="EfmGK923_31490"
     CDS             3311..4804
                     /locus_tag="OQH82_RS15875"
                     /old_locus_tag="EfmGK923_31490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            4939..5226
                     /locus_tag="OQH82_RS15880"
                     /old_locus_tag="EfmGK923_31500"
     CDS             4939..5226
                     /locus_tag="OQH82_RS15880"
                     /old_locus_tag="EfmGK923_31500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="WP_265145944.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANEGSVAKF"
                     /name="type III secretion system protein PrgN"
     gene            5280..5960
                     /locus_tag="OQH82_RS15885"
                     /old_locus_tag="EfmGK923_31510"
     CDS             5280..5960
                     /locus_tag="OQH82_RS15885"
                     /old_locus_tag="EfmGK923_31510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(5990..6688)
                     /locus_tag="OQH82_RS15890"
                     /old_locus_tag="EfmGK923_31520"
     CDS             complement(5990..6688)
                     /locus_tag="OQH82_RS15890"
                     /old_locus_tag="EfmGK923_31520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265169994.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSVLLQSFK"
                     /name="hypothetical protein"
     gene            complement(6742..7116)
                     /locus_tag="OQH82_RS15895"
                     /old_locus_tag="EfmGK923_31530"
     CDS             complement(6742..7116)
                     /locus_tag="OQH82_RS15895"
                     /old_locus_tag="EfmGK923_31530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            7249..7710
                     /locus_tag="OQH82_RS15900"
                     /old_locus_tag="EfmGK923_31540"
     CDS             7249..7710
                     /locus_tag="OQH82_RS15900"
                     /old_locus_tag="EfmGK923_31540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8024..8194)
                     /locus_tag="OQH82_RS15905"
                     /old_locus_tag="EfmGK923_31550"
     CDS             complement(8024..8194)
                     /locus_tag="OQH82_RS15905"
                     /old_locus_tag="EfmGK923_31550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(8210..8746)
                     /locus_tag="OQH82_RS15910"
                     /old_locus_tag="EfmGK923_31560"
     CDS             complement(8210..8746)
                     /locus_tag="OQH82_RS15910"
                     /old_locus_tag="EfmGK923_31560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002303560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(8767..9711)
                     /locus_tag="OQH82_RS15915"
                     /old_locus_tag="EfmGK923_31570"
     CDS             complement(8767..9711)
                     /locus_tag="OQH82_RS15915"
                     /old_locus_tag="EfmGK923_31570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            10348..10434
                     /locus_tag="OQH82_RS15920"
     CDS             10348..10434
                     /locus_tag="OQH82_RS15920"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(10555..11244)
                     /locus_tag="OQH82_RS15925"
                     /old_locus_tag="EfmGK923_31580"
     CDS             complement(10555..11244)
                     /locus_tag="OQH82_RS15925"
                     /old_locus_tag="EfmGK923_31580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303565.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(11246..11524)
                     /locus_tag="OQH82_RS16145"
     CDS             complement(11246..11524)
                     /locus_tag="OQH82_RS16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(11484..12398)
                     /locus_tag="OQH82_RS15930"
                     /old_locus_tag="EfmGK923_31590"
     CDS             complement(11484..12398)
                     /locus_tag="OQH82_RS15930"
                     /old_locus_tag="EfmGK923_31590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002303564.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(12380..12760)
                     /locus_tag="OQH82_RS15935"
                     /old_locus_tag="EfmGK923_31600"
     CDS             complement(12380..12760)
                     /locus_tag="OQH82_RS15935"
                     /old_locus_tag="EfmGK923_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002292464.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(13202..13339)
                     /locus_tag="OQH82_RS15940"
                     /old_locus_tag="EfmGK923_31620"
     CDS             complement(13202..13339)
                     /locus_tag="OQH82_RS15940"
                     /old_locus_tag="EfmGK923_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(13453..14073)
                     /locus_tag="OQH82_RS15945"
                     /old_locus_tag="EfmGK923_31630"
     CDS             complement(13453..14073)
                     /locus_tag="OQH82_RS15945"
                     /old_locus_tag="EfmGK923_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(14070..15266)
                     /locus_tag="OQH82_RS15950"
                     /old_locus_tag="EfmGK923_31640"
     CDS             complement(14070..15266)
                     /locus_tag="OQH82_RS15950"
                     /old_locus_tag="EfmGK923_31640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002352126.1"
                     /translation="MILLLIASTKVINIHNEAENNSLKYWERTTNLYKTNITNQLNRNN
                     TVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFIGQKVYPNFEDYVSQP
                     AGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYKEYAKMIEKNYLEDI
                     QFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKAKDNYTITDPIVRVIN
                     PEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNLENIINFSYSVFQETG
                     ELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIALKKIFGYSPISQVLD
                     LCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIIIYKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            15605..16285
                     /locus_tag="OQH82_RS15955"
                     /old_locus_tag="EfmGK923_31650"
     CDS             15605..16285
                     /locus_tag="OQH82_RS15955"
                     /old_locus_tag="EfmGK923_31650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            16483..17088
                     /locus_tag="OQH82_RS15960"
                     /old_locus_tag="EfmGK923_31660"
     CDS             16483..17088
                     /locus_tag="OQH82_RS15960"
                     /old_locus_tag="EfmGK923_31660"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            17104..17676
                     /locus_tag="OQH82_RS15965"
                     /old_locus_tag="EfmGK923_31670"
     CDS             17104..17676
                     /locus_tag="OQH82_RS15965"
                     /old_locus_tag="EfmGK923_31670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002303206.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(18149..18721)
                     /locus_tag="OQH82_RS15970"
                     /old_locus_tag="EfmGK923_31680"
     CDS             complement(18149..18721)
                     /locus_tag="OQH82_RS15970"
                     /old_locus_tag="EfmGK923_31680"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_073120187.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREV
                     EQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGF
                     YNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="IS3 family transposase"
     gene            complement(18822..19277)
                     /locus_tag="OQH82_RS15975"
     CDS             complement(18822..19277)
                     /locus_tag="OQH82_RS15975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_010721070.1"
                     /translation="MDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     BGC             1742..12116
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3312..4804
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8768..9711)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11488..12398)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcttctaatg ggttgtctct taagaattta cgattcaata cgtttgtcgt tttggcttca
       61 ttgcaagtag aatgatgtac tttaaaataa gcaacagtat agctcgattt taatcctcta
      121 tttttcatga ttctactaat ttttcgtcgg ctgatctgaa tgcctcgttt tgataaggct
      181 ttttttattt ttcttgagcc gtaggccttt cggctgcgga taaattcttc agcgactact
      241 tcttcaagtt ctgattcgtc tttctttggt tttgattgat aataatagga ctgacgtgat
      301 agacctaata ttctgcacat cgctgatata gggtaaagat gcttattcgc atcgattact
      361 tgtctcttcg tccgaatatc agcgctgctt gctttaaaat atcattttcc atttctaatt
      421 gctggtttct tttacgtagt tctaacaatt ctttttgttc aggcgtaaga ttatcttttt
      481 ctttgaatga accactcgtt ttaaattgct ttacccattt gtcaaatgct gaagccgtta
      541 gttcatattc tcgaatgatt tctacacgtg gctttccagc taagtaaaga ttgacgattt
      601 gttgcttgaa ttcttgtgaa aaagttcttc gtgttctctt agacataaaa attcctcctg
      661 gtatgttttc ttctagtcta cacaccttaa tttttctgtc tagttaattg tagcctatcc
      721 atgatcggtt tattagacct ttgaggtcta tattttttaa ctacaatcga ccttaaattt
      781 aattgtttca ttagcttttg gacatgttta agagatactg aaatcccttc ttttaataaa
      841 acttgatgga tctttgtggc accatagcgt tgtttattcg taaaaaaaat tgttgaaatc
      901 ttctttttca acttattatt tctgatttct gtagctgtca acggtttatt cttataaaaa
      961 taatagacac ttctagggat ttctaataac cgacacaaca aagaaacagt gtaatcctta
     1021 caccattttt caataaattg aacaagcgaa tttaggtttt ccgagagaat atggctgcgg
     1081 ctttttttaa gatttcaagt tcctcattta aacgagcatt ttcttttctc agttcagcta
     1141 cttcttttcc agtcagtcct gtggactgat caggtaaata taaattcttc catttgtaaa
     1201 tcgttgcttc agataagcca tattctttcg ctaaccctcg aacagaacgt ccagtttgat
     1261 tcagttcaac aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc
     1321 tcgtagataa gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa
     1381 caccaaatgt tgtatacccc caaacaatac acatattttg aaggattagg attaataaag
     1441 ctaaaaatat agtaagtata ctaaatatat ttactatatc tagtatactt actatatttt
     1501 aatcttttga tcgtctatta tatataatcc gttcctcgtc gtttaaagta tcaacatagg
     1561 cgtccattgc aagtttgact atttcataca taggaatatc ttttgcataa ctaagatccc
     1621 gaatggtttt tagtacggga ggatctactt gaactggttt tctttctttc gcagtgtact
     1681 ttcgttcccc taactcgaca ttaatatcat ctttaattga aatatcttca gcaccaattt
     1741 tcttcttatc tttgttcgtt ggtaataatg ccatttagtt taatctcctt tccaagaggt
     1801 aggatatgat tggccattga gtcagcatat ttaggtgtat aatcaaatcc ttcaatatca
     1861 ccatgttgtt catagtattc aattctttct tctaattctg taaagatgtc tgcaaaaata
     1921 gcccaccatc ttcgatctac ataatcattt aattggatac cattctcacc atacatttct
     1981 aaccgatccg agcctttaac aatgctttta aaaatgtttg attcaccata cttatcaatt
     2041 gttttttgat agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac aggcaaaatt
     2101 ccaatgattt gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa aactttatta
     2161 ataaaatctt ctgttccttc cattgcgtac cgttttaatt cttgcattgc aataatataa
     2221 tctgcagcag ttaagaatgc acgaaccaca ttatctgttg atggtgggca gtcgataaag
     2281 atataatcga aattttttct caattcttct aaaggtttaa tgaaggccaa tgatctctta
     2341 cgacgatcag gatatttttc ggacaagtat tcattaagtt cttcagatcc aggagagcca
     2401 gccataatat ataaatgtgg acttacttga gtaatacctg aaacccagtc cccttttttg
     2461 attgctgcag acatggatcg atcaaagcta gtaatttgat acgttgatcc aatgttggaa
     2521 gtagtatttc tttgttggtc ataatcaatt agcaacacgt ctttttcaaa aagttgacta
     2581 gaaacaatcg ctaataaatc attgtctgta gtcttaccaa tgccgccttt tctacctgta
     2641 agcattacaa cttttcctag tttatctgat gttttaaatg cagtccgtaa gttttccata
     2701 gttccctcca atttcctata ctaaatatat ttagtatact tcatatactt aatatattcg
     2761 atatatctaa tatactccaa attgataaga attcagactt tataaggaat atagtggata
     2821 tactaaatat atttgctata ttcagtatat cagatatatt caaaatgtca aaatatattt
     2881 gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa cttaattcta tagaaaccac
     2941 agataattca ctatttacaa agtagcaaaa gcagtggtac tctttaaaca gaaaaattac
     3001 ataaaaaaaa gagacggaaa taattgattt tagcagaatc aattatttct gaatcacatg
     3061 gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa ctacttatat
     3121 taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa ggcgagcaac
     3181 tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca ctgaagagag
     3241 aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt gagaaaggac
     3301 gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct tacgtacata
     3361 caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta ggagtggtgc
     3421 tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg tcattacttc
     3481 ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta atacctatcg
     3541 ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg agaaaaactt
     3601 aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc aaggcgaaat
     3661 tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag aatcagatca
     3721 tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga aaagcaattt
     3781 caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa
     3841 attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc cacaagaaaa
     3901 taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga ttcaatggtc
     3961 gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac taattgtctt
     4021 tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa
     4081 agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt tcacgctttc
     4141 tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata tgttcacatt
     4201 caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg tacgtagtgc
     4261 ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt gccagcaatg
     4321 ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct ggtggaaatt
     4381 taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag aagatctatt
     4441 ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt caaaaaaaga
     4501 attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa atgagttacg
     4561 agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa tgatcattgc
     4621 ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga gactagatta
     4681 ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag agacatttag
     4741 acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag tagatacagg
     4801 ttgaaaaata aaaggtgcaa attgccttat acaatatatc tatgatactt ctgtaggtgt
     4861 tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg cagtcttatg
     4921 tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc tgttaatgtg
     4981 tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta tggttttaag
     5041 caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt gccgattgag
     5101 tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata tgattgtttg
     5161 tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagggttc tgttgcaaag
     5221 ttttaaatct actatcaaat aaggtagaat aatagaaaaa gatagcagga ggaatgacga
     5281 tgaatcattt taaaggaaag caatttcagc aggatgtgat tattgtagcc gtgggctact
     5341 atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat cgtggcatta
     5401 acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta ctctatcaaa
     5461 tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa acgtacatca
     5521 aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt ttaaccttgg
     5581 atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt aagcggttag
     5641 tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct attacaagtg
     5701 cctttaagaa actaaaagaa tacggctttt atcaagggac agaacatcgt accattaaat
     5761 acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat aaattctatc
     5821 gaagtttacg cactgcctct accacgatta aaggcatgga agccattcga ggattatata
     5881 agaaaacccg aaaagaaggc actctcttcg ggttttcggt ctgtactgaa atcaaggtat
     5941 tattgggaat cccagcttaa atcatagata ccgtaaggga ttttattctt tatttaaaac
     6001 tttgcaacag aaccgaatct tttatagtat caaaagagtt atagcctaac atttttttta
     6061 gagtgatttc tttcattttt gaaaaagcaa atataattgt agtaattaca ataaatatac
     6121 taagatagca gataacaaga acaatattag tattaatttg aaaagtatct gattgattga
     6181 tattggaaat agtaggtgtt ccatattttt tcagtacagg aattaatttg tttgagtttc
     6241 cttgtacata taatatttca cttaatccaa tattttccac atgagaaaaa ggataaatat
     6301 ttatatttaa aaaattattt ggaagtttta attggtactt agaatttttg ttattagtat
     6361 aatggacttt agaatcgttt cttgctaata tatttttttt agaaaatttc cagtgtttgt
     6421 tagcgttagg gtttgtagca agaatagtta gttggttaac agcagtaaaa ctatattgag
     6481 aaatattaat attttcttgt gtagatattt catacaaagc gtttatataa ctgttaaagt
     6541 ccatatcatc tttatattgc atatgaattt cagcggtatt tttatatagc gcttcagaag
     6601 tctttatacc atttataaca ttagtagaaa agaatagtat tgtaatgcag acaaaaagtg
     6661 ataaaacagc gtttattttt tttaacatat tttgaatttc ctttcaaatg taaataaaaa
     6721 agagagttta aactctcttt tttaataaac attccaccaa ccagtatttc cagtaagtgt
     6781 ggcattgcat gttgcataag ctcttactcc agctttctga actgatcgta cttgaccgcc
     6841 tcgtccattt ttagctgttg cactatgttt agctctagca tggtagtaat ttgcataaac
     6901 tttaaatcca ttaaccccac agttgaaatc tccgccgcca gcagcatatc tcaatttact
     6961 agaagaaaat ccttcttctg tagcttccat tcctcctccg taaatggcgg tatggtcttc
     7021 gggtacatct agatttaaag cttctgtagc actaactatt cctccactag ttaataagac
     7081 acccattaaa gcagaaagac ataaaaattt tttcataact tatttctcct atctttttta
     7141 ctagaattat tttattttag tgtgttttat aaaataaaga acttttttgg cgcgttagta
     7201 tttttatgat aaaattttat gtaaataatc gtttacatag gagaaaaaat ggaggattta
     7261 ggatctaagc taagagaatt aagagtgaaa aaagggtata ctcaagaata tgtggcaaga
     7321 aaatttaatg ttacacgtca aactatttca aagtgggaaa ataacaaaac aacaccagat
     7381 atagagacac taaaaaaaat ttgtgaattt tatagtacta acataaattc tattttaaat
     7441 gaagataatt taaaaaataa acaaaataat ttactggtat tgacagctat tatttttaat
     7501 atatttgtta ctatattagg tttatttgaa ttgattcaaa tgaatttttt tagctttaca
     7561 cacggaatat ttacttggtt tgctctatct ggattaatag taatagatat cttaaattat
     7621 ttttatattt actattctta tttaaaaaat gaaaaatttt tttatacaat attttttccc
     7681 ctagtgattt tgattatttt attaagctaa tatgaagtag ttagttattt aattgcatag
     7741 ttgacataat gtacagtatg ctaagtagcg cctgtatcaa tcgatacagg ggctcacttt
     7801 gtgattttta attggtctag gtcaaaagct ctttttaagg cttgattttc tgcagttgct
     7861 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt attttttata
     7921 aaatatgata aaattttggt agcgctttat aagcgcgttg ctttttcaag aataaactct
     7981 cattattttt ttaactggct gctcgaaaga gcagtctttt ttattattta tccgtttctt
     8041 ttacaaacaa ttcttcgtta ggactgttgt aataatttgg attcaatttc aattcaccat
     8101 gtttattccc tttattgatc actgacaata acatcggact gaagcctaga attcctagta
     8161 aaatagaacc gaatagaaga ttttttcttg tcatgaatat tccctccagt tatttccaaa
     8221 gttgccacca tttttgcttc ttctttgtgt gtttttctat cgattctgtt tcttcagaaa
     8281 gaatagtagt tgattcatct tctggagttt cttccgtata aactaattgt aactgttctt
     8341 gcagtttttc gatctgttga tttgcctgta gggtgagctg ttgttgctgg tcgagtaatt
     8401 tttgcatatt agaattattt tgctctttaa gggaaagttg ttctcgtaaa aattcatttt
     8461 cttcacgcaa cttatctagc aactcgttta caaccacgtt tttgcttgat atataaatgt
     8521 ttttatcgtt gtctttttca tactcatgca acctatctag taacctttgg ttacctaagt
     8581 tgctagcaag ttgcaatact ccagaacgca atacaacctt ataaccacgc tcatttttag
     8641 ctatcatgga agaatctaat ttttggatat gtttcgatat tgcttgccta ctcatcttaa
     8701 aaagatctga caattccttt attgtaaagg tttcttgtgg ttgcatactg gttgcctcct
     8761 aggttgctac tggaataaat tatctaaatc agataataaa ctttttttct ctttttccga
     8821 aagtgcggac tctgtttgtt tttcaagagc ttttttctct ttttctagtt gtttgatttg
     8881 ttggaggagt tcaagttctc gtttcttctg tttgattttt tcgttaattg ttgtagcggg
     8941 ttcttcgtat tctttttgtt tatcagctaa agccaatgct ttattttggt cttctttggt
     9001 tggtgcatgt ttgttaaagt cgtctgcttt tttgatctca ggtttgaacg taaattgata
     9061 gccaataaca ggttttcccc gtccttttcc gtatttcttt ttgattgtta gtcctttgaa
     9121 gattgcagtt agttcttgac gtataggttt tataactcgt ttatctatat caggttgctt
     9181 ataactttta ggaatatcta ataattcaag aaagtcttct ttggagaaat atgcgaaacc
     9241 tttggttctg tattgcttta acagacgaaa catagtcttt gcatagccac tttgtagctc
     9301 gttgaattgt tgtaaagaat agcgtaccca ctcgtctaaa ttatttaaca aaggcagtgc
     9361 tttttcgtaa attcgtatat ctacgtaagg attttctacg tccccgttga ttttaaattc
     9421 tgtaaacatt acaaacatat cgtaagaaag cccagactca cttctacgac caaagcgtaa
     9481 agataagatt ttttcataag ttgcttttaa atcttctaca aatcgtacat ttgcagttgc
     9541 tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct tatccccttt
     9601 ttcacgcatt ctagacacga tggaaaaaaa caaattcatt tcaaccggtg taaattttct
     9661 taaaggaatt gtatttaatt catggtggta ttttacaatc tcattagcca tataatcacc
     9721 tcaatccaat tatgaatgac attttttaaa tgtcaagaca ttcatatttg acgttcaaac
     9781 agacaaaatg tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc
     9841 ataaacagac aaaatgtcct tcataacatt ctgtatccat tggtagagta aggacacagc
     9901 ttacggtaaa gaggtttaaa aagaggttta aaaagagata taaaaagagg ggggctaatt
     9961 ttcccgccta aaaaccactt cttttttctt taaacaggtt cgcgcatttt tcaaaactgc
    10021 caatccaaaa ccttgcttcc tacggtcgcc aaaaaggtgt gatcgctacg ctctcacatt
    10081 gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacat gttggtaaag aggcctttga
    10141 gcggttgtga atgcttatct aagccgtttt aagacagtct tggataaaaa tccttatcta
    10201 gctttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaaa taagcgatac
    10261 ttaaaaacac cacacaagtt taatttttta agatataata acttgtcgcc tatccgacag
    10321 tcttgcgact ggaaggaggt gtatctaatg tttgaattgt tcatttgccc ccttcttata
    10381 gaagtttgtg tctctttatt cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa
    10441 taaaggcgac atcaacctga aaaaaagccc cagagaatcc gacctctctg gggctttcgt
    10501 gtatctaatg tttgatcatt tgcatgttta gtttaacatg attgccgatc aaattcaatt
    10561 ttgctgttgc tgatctaacc attccttgac actttctaga taatttaatg ccgtttcttt
    10621 tttcgttgga ctttgtatgt tagttgcttg ttgaaacagg ttttcgtagc gttcatatgt
    10681 gacagattgt tgtttttctt ttttttcttc ttcactggca aaccaagggt ttgtgtctcc
    10741 aaatagatca ctacaggctt cttctatctt tgtatctttt acatattgca cgtccttttt
    10801 tacttgttca tatttttctt ggtattcttt ttgcggttct tctggcaaat tccgataaag
    10861 tcgagtgagt gtttccatat ttttatccgt caaacgcttt tcttgcttca caaaaatatc
    10921 attcatgtcc tcataacaag cggtaaattt ttctattttt tgtgctaagg cttgctcttt
    10981 tttcttttta ttacgtgctg attcatactt ttgagtgaca tttcctaata aaatacttct
    11041 agaaacgcgc ttattgccct ctaaaacgcc taataagccg cttgaaacga ttatcaagga
    11101 aaatatacag ccaagcaaga aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata
    11161 tcttgtttgg aaaattttat agatgagcca aatacttcct gaaatactaa caaaaatgag
    11221 tagatagggt aaataaaatg ccattttagc ctccccatac taaccaaaat gcgaacagtg
    11281 tcaggactaa gcaacataag ggggcaagaa aatccattga tcgttgaatg aacgcctttt
    11341 tttgttcttt atttcgctga tctaatagct cttgatggag ttttttaact tgtaaatcaa
    11401 tcgtagtttg tatcctttct tgtgtgtagg gagcaagaaa agcacgttgt ttctttattt
    11461 cgttttctat gtcttttaac gttttagagc gataattctt gttggtggtc tgttcctctt
    11521 tcaacgtgtt gttcatggtt tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga
    11581 gttcgttgtt ctcttcgtac ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt
    11641 agctcgttgg ttcgctgttg gttataggtt cgttctcgtc tgtccagctc atgtaaaatc
    11701 gtctcctttt cgtagtcctt tcctagtctg ttggatcgta cttttttctt tttgtggatc
    11761 aattcgtaag tgaaattttt cccgcgattg aagcaatgaa tgccgaatgt tttgagggaa
    11821 tcacaaaatt ggtcataggt tctggttttc ttgtttttca tggtttgatc aatcgcaaac
    11881 cgaatttcat ctttccaaac aggttcattt ttttctttta ttttgatttc agccatggtt
    11941 cgtttttctt ctttttttcc tcgttcagtg atacttaaac catgagttaa acaaatttcg
    12001 tcgttgatat cgtaacactt atctaggaat gcttgaccat gtgcttgaaa cttttttcct
    12061 gtttctaaat tgaccgaatt aatgacaatg tgattgtgga tatgctcttt atctgtgtgc
    12121 gtgtaaatcg cgatttggta ttctggcgcg attttttcag cgtattcttt ccctagttgg
    12181 ttggcttttt cagcggtaat ttctcctggt cgaaaggact gaatcaataa atgtgcttgt
    12241 acacgatcgt cttttccata aagcatgcgt atttgtttca tttcactttt ggcataatca
    12301 atgtcacagt ttattcctgt tttgacggtt gctcgtggtt ctgcgtaatt gattgctcgg
    12361 ctacatgaag tggttcgact tactttaact gttgccatat cttttgtagc tccttttgca
    12421 tgatttttag gttttcggat aaacgttgag cgtcaccagg agcaacgtca tgttggtgtg
    12481 tattgcacca cttagcaatt tgattgatat tccctccagc tttggctaaa tcgcgcgcga
    12541 ttgcctgggc aatgtctggc gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg
    12601 ctttcacgaa agccggcaca ctcatttgca aagcttcagc tgactgcttc agctttgcaa
    12661 attcggattc gctcactcga aaactgattt gttttggctc ggatcgatag gtatattttt
    12721 tagaaggatc gctagccaaa ttttgatttt gttcgctcat aagtcccacc ctttcagtac
    12781 gtcctacgga cgtatcgttc gcaataccat tatacctaaa aaggtacaat gagtaaagct
    12841 cactaaaagg gtttggcaag ctctatattt gctagccaaa tacctccgta tttggtgcaa
    12901 ataaccgctt gtcaaagggg cgcaagctcc cccttgaatc cccctcaaaa cccacgctcc
    12961 acaatacaaa aaaagctagc aaatgctagc cactttttgt aaagtttcgc ttatttcacc
    13021 gaagatgaaa ttgttgcttt gcaacaaaaa acataaatgc ttttttgtgt aaactactaa
    13081 ctatttagct aaaactgata gttttattaa acattcagca atatttttag ccaatttttt
    13141 atcagacttt cggcgagtca ttatgataat atgtccactt tttgttactt ctaccaaaaa
    13201 tctagcaata actctcccat tcttcagttg atttagtttc ataagtcttt tgtaattgac
    13261 ggtttaattt tttgggcaat tgcaaagaat aaataagtct atcataaaat ttttcatact
    13321 cttttttcaa taaaatcact tgggtgcctc ctttttatta agtgattatt taaattttgg
    13381 gataggagag atggaatata aaataaatac ccgtggtgct agttttaaat cagctagcat
    13441 cacgggtaaa aattataatt ctataatatt agttccatag tttagaacat ttaagtcatg
    13501 tgttactagt acaacggctt ttttttcttt ttctgttaaa tttttcagaa agttcattat
    13561 aaaagttgag ttttctttat ctaaatttcc tgtaggttca tctgcaaata atatatcaaa
    13621 tggttttaac atatttctaa ttaatgctat tctttgttgt tctccaccgc ttaagagaaa
    13681 gattttttct tttaagatat cttttggcaa agaaaaatct gttaataaat tataaatatg
    13741 atcattggaa atattactgt taaacttttt tgctaattcg aaattgtatt gtactgtttg
    13801 attttcaaga agagcatagt tttgaaataa atagctaata tgatttcttc gtagacgttg
    13861 tatatcatgt ttagaaactt tcttttcaaa atagaataag ttaccggtat attgtttatc
    13921 tattaggctt aaattattta acagagtagt ttttccacta ccactttttc ctgttactat
    13981 aataaaatct ttttctgata ttgaaaagtt ccaattttgt ttgatctttt ttttattaat
    14041 tgacagggtg aaattctcaa ctgataatat cataaaatat tctccttaaa atatttaaaa
    14101 ctattttttt tataaataat aatagaagta attagattta ttaaaaagat cataaaaatt
    14161 ataaaataca agtaagtgtt ttttgaaaat atcaattgaa aacacatagt agcaaataac
    14221 tctattgtaa aagggagtaa acataaatct aaaacttgac ttattggtga atatccaaat
    14281 atttttttta aagctatttt ttttgaattt atagtaaaac taacaaaaat catttgaaaa
    14341 ttaattatta taaataatcc taatattaaa agatattgta gatatgcatt taatttattt
    14401 actttttttt gagataataa ttcaccagtt tcttgaaaaa cagaataaga aaaatttatg
    14461 atattttcta ggttaaattc ttttatatac ggattaattt gattaaaaaa gttttggttt
    14521 gaagtatgtt tctgatcaaa aaataatcca ccatcactag tcaatatatt tccccaaaat
    14581 aatgattctg ttatttctgg attaataact ctaactatgg gatcagttat tgtatagtta
    14641 tcttttgcct ttccatataa ggtattaaaa ctaaaatact tttggttatt attaacaaat
    14701 attagtttta tttttaaatt tttcaaatta ggagttttat aaggagcttg aggaggagag
    14761 tcttcaagtc taaactgtat gtcttctaaa taattttttt caatcatttt tgcatattct
    14821 ttatattttt taggtactag aatgtaagta gttagtggat cagagtttat taaatttaag
    14881 tcagtagatt cttcaaaaga aataggattg taatatttta gatagtttaa atcaacacat
    14941 attcctgcac cagcaggttg actaacatag tcttcaaaat ttggataaac tttttgtcca
    15001 ataaaaaatt ctttattatt ttcttggata gttgcgtaat tgtatggcgc gataataaaa
    15061 gttttataat tcttctgtat tttataaaca aatttactag cttttttcaa gtagttattt
    15121 tcttctacgg tgttgtttct atttaattga ttagtaatat ttgttttata taaattagta
    15181 gttctttccc aatatttaag agaattattt tcggcttcat tatggatatt tattactttt
    15241 gtagaagcta ttaacagaag gatcatgctt aagaatagac agatatttaa tatacaaaga
    15301 tttaacttaa gtggtcgtga attttttaga taatttttta tactagataa atgatagata
    15361 cttagagtaa ctatgataaa taaaaaacta attataaaat gaaaaatact acatagaaga
    15421 aatgatagga aaaaattttc gttactgtaa tatatgctca aaaaaataga taaaatgaaa
    15481 gttataatca aagaacaaaa taagaggttt aactctttaa tagaatcttt ggttctgttg
    15541 caaagtttta aatctactat caaataaggt agaataatag aaaaagatag caggaggaat
    15601 gacgatgaat cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg
    15661 ctactatctt cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg
    15721 cattaacgtt tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta
    15781 tcaaatttgg aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta
    15841 catcaaaatt aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac
    15901 cttggatatt tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg
    15961 gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac
    16021 aagtgccttt aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat
    16081 taaatacctg aataatttga ttgaacaaga ccatcgtcca gtaaagagac gcaataaatt
    16141 ctatcgaagt ttacgcactg cctctaccac gattaaaggc atggaagcca ttcgaggatt
    16201 atataagaaa acccgaaaag aaggcactct cttcgggttt tcggtctgta ctgaaatcaa
    16261 ggtattattg ggaatcccag cttaaatcat agataccgta agggatttta ttctttattt
    16321 aaaactttgc aacagaacca actagactgt gcaaaataga gacacttgaa aaatctatcg
    16381 ggggtcagaa acttaatagt ttttgacaca taaacaaaat cgcgatataa tggggttgag
    16441 agtgtttcat aaacttatgt cataaagtta aggagatttc gcatgatttt agagaataaa
    16501 ttgggattaa ccaatcaagt tgagttggct aaggttgaag aaaaactcag taaacaaaaa
    16561 gcgaaagaat tgtacgattg tggaaaaata aatgaaatcg aagttggtac atttaaaggt
    16621 ttatctgaaa ttcatgaatt tctattttcg gatatttacg attttgccgg aaaaatccga
    16681 tcggtcaata ttgcaaaagg aaattttcga tttgcaccag ttatgtattt agaacattcg
    16741 ttacagcata ttgatcagat gccacaaact tcttttgaag agattatcaa gaaatatgtg
    16801 gagatgaata tcgctcaccc atttcgagaa ggaaatggcc gaagtactag aatttggctg
    16861 gatctcattt taaaggaaga attgcaaaaa gttgtagatt ggaacttaat cgataaagca
    16921 gactatcttt ctgcaatgga gcgaagtccc ataaacgatt tggaaatacg ctatctaatt
    16981 tccaatgctc tgactgataa aattgacgat cgcgagttat atatgaaagg aatcgatgtc
    17041 agctatttct atgaagggta ttctgagtat acgatagatg atttgtaaaa gtaggtggac
    17101 caaatgacga aatatggata tgcacgtgtc tcaaccgctt ctcaaagtac aaaagaacaa
    17161 attcaacagt tgcttgagaa ctgcgtagag aagaagaata ttttttcaga aaaatttacg
    17221 ggtacaaaac gggatcggcc aaaattcaat cagctgttgg ctggtctgaa gcctggagat
    17281 gaaatagttg ttacgaagct tgatcgattt gctcgtaaca cgagagaagc attagagatc
    17341 attgaacctt tactcgatga taacgtaacg atcaaggttt tgaatctagg aacgattgaa
    17401 aatacgtcca tggggcgaat ggtgacacgg acgttgttga gtgtagcaga aatggaacgt
    17461 gatatgattg tggaacgtac ccaagaagga aaaatgtttg caagaaagaa taatcctaat
    17521 ttcagagaag gacgtccgaa agcaacaatc acaccgaaga agaggcacgc atatgaactg
    17581 cttacttcag gtaaatcgta taaggaagtt gaagctataa ccggtttcag tcgcagtacg
    17641 ctttttagaa taaagaagaa aattgaagaa agttaaaaaa gaagtctcag cgaaccagac
    17701 aggtaaatac ttaatgcgtt ctagatatga gtctattagc atgttgtgaa atataatatt
    17761 tttttattta cttgatttct gctcaaaaaa tgtgtatggt aactgtaaaa ggtaaagtaa
    17821 ttaaaggtat gctagccacg cttactatat taggatacca tcatatattt ataatgaacg
    17881 atttgttcgt ctaccaaaca acacatatcg ttcagcgtaa gtcacgttta ggaacgtggc
    17941 ttttttagtg ttattatcga taaataatct attgactcta ttgattttat ctccatatcc
    18001 ctacggtgtt gattgtcttt agatctttca tttaagaacc aaataactat cataaaaatc
    18061 agcactgctg ctaatataat aaacagcatt cctattgtga tattactgga tttgagtcaa
    18121 tatcttggac acattttagt agagacggtt aagccatttt ttcttttgct aattcttcaa
    18181 attcctgtgg ggttagatag tgaatcgagc tatggattcg atttcggtta taaaatcctt
    18241 caatgtagct aaatagcgct cgatttgctt cttcaaaatc tgagtagcta gtcgtgtaga
    18301 cttcttcttt tttcaatgag gcatggaaag attcgattcc agcattatca taaggtgttc
    18361 cttttctact ataagaatgc cttattttgt tggttttaag ccattgttcc acttctcttg
    18421 ctgtgtattg gctgcccaag tcagtgtgta gaatcattcc ttctggaatg tgataatgta
    18481 tttttgcttt attcaatgtt tgaatgacac aatccacagt cattcgtttt gaaaatgtat
    18541 aactaataat tcttttcgtg tgtaaatcca tgattgaaga taagtaacac caaccatttt
    18601 tcttagtagg aatgtacgta atgtcagctg cccatttctc acagatcgtt tcagtagaaa
    18661 agtcctgatt taagagattc tcttttgaaa tgatggaatg gcaacagttt ttttgacaaa
    18721 ttttataagg tgcagaactt ctttccgtat gctattccga ttgtccttga caatgagcct
    18781 cctcggatgt gacgatcttc ggcaggagct aacagttaaa gttagactgc ctcgctagcg
    18841 ttagcacatc cgagctcatt atcaaggatt cgctgcgcca atctctggtt acggtaacca
    18901 accggtgtct cgtagccaag tgtaccgtgc aaccgaaggt ggttccacca attgacatag
    18961 tcaaataact ccaaatccaa ttgttgtaag gtttcaaatg tgtattgata gacaaattct
    19021 actttcaacg acttataagt tgattcagct acggcattat caaaaggaca gcctttatga
    19081 ctcaatgatc gattgatgtc aaaagttgtt aataattcat caatagcttg gttatcaaac
    19141 tcttttccac gatcagtatg aaaaatctca acctctgtca gaggttgttt gatacggcta
    19201 aatgcttttt ttactagaac ggcatcttta tgttctccac aagaatagcc gagaatttct
    19261 cgattgaaca gatccaaaat gaaacagaca c
//
