LOCUS       NZ_AP026431             2325 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645
            plasmid pKAM645_11, complete sequence.
ACCESSION   NZ_AP026431
VERSION     NZ_AP026431.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446298
            Assembly: GCF_024347815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 101x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:42:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,116
            CDSs (total)                      :: 6,009
            Genes (coding)                    :: 5,826
            CDSs (with protein)               :: 5,826
            Genes (RNA)                       :: 107
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 77 of 183
            Pseudo Genes (incomplete)         :: 115 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 35 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026431.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM645"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM645_11"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            join(1938..2325,1..50)
                     /locus_tag="OCV84_RS30525"
     CDS             join(1938..2325,1..50)
                     /locus_tag="OCV84_RS30525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_261902568.1"
                     /translation="MGNSKRNINKLNENFREDILDYAIAHNLKCANALAILYATGCRPD
                     ELHTGVKINYDKQKNEIRFKIIGSKLNRRMKRGIGIREFSVKINNDNERFFKGIIDLIN
                     ERPVDSFDHNFKIESAKAFSGYITKIIMLLHILSGMQKQLS"
                     /name="hypothetical protein"
     gene            47..316
                     /locus_tag="OCV84_RS30530"
     CDS             47..316
                     /locus_tag="OCV84_RS30530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_261902569.1"
                     /translation="MKNSDYDKEKIAQIMGHASVRSQQSYGRKNKKSKGGFNDIEDIKT
                     NVKPRGADRLLRFKIASKNQAAAKIADTSTPSSPPPAPVRRFKM"
                     /name="hypothetical protein"
     gene            822..1523
                     /locus_tag="OCV84_RS30535"
                     /old_locus_tag="KAM645c_59470"
     CDS             822..1523
                     /locus_tag="OCV84_RS30535"
                     /old_locus_tag="KAM645c_59470"
                     /inference="COORDINATES: protein motif:HMM:NF017542.3"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_261902570.1"
                     /translation="MDLGNILNESLSIDYEKLDLFLEKYDLTPEQKVAVYEFHAKAYKK
                     NKTLVISETKENKFKSISEGVEYVHLFPKNLKILIKKYGLNTNELLVLTEIMESMLSHG
                     NLLINFSQKALCELTGINKSTMCKTFKTLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFM
                     QFRDHFLNSISYKLDDEEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFE
                     KEISERMERKV"
                     /name="replication/maintenance protein RepL"
     Typing          377..561
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="1.6600000000000002e-73"
ORIGIN
        1 ttatcatgct tctgcatatt ctttcaggca tgcaaaagca actgagttga aaaattcaga
       61 ttatgataaa gaaaaaatag cccaaataat gggccatgcg tcagttagat ctcagcaaag
      121 ttacggtaga aaaaacaaaa aaagcaaagg tgggtttaac gacattgaag atataaaaac
      181 taatgttaaa ccccgtggtg cagatagatt gctaaggttc aaaattgcca gcaaaaacca
      241 agcagcggca aaaattgccg atacttccac ccccagcagt cctccaccgg ctcccgttcg
      301 tcgctttaaa atgtgaaccg tgagcagttc aggaggtccc ctcctggact gtgaagggtt
      361 ggcctggcgg cgagccggtt ttctagcaaa atcctccata ttgcgaagca gggcccggaa
      421 ccgggtaact ggatggtttt cccctgtggg ggattgatct gttacttgaa aaccaatgat
      481 cttaaaaacc atctcaaaag ttgaaaattt caccccctta gtgttcttaa aattcttaga
      541 tgttcttagg ggtttaaaaa ctactctcca accattgatc taactgactt tttaaaaaag
      601 gcagttgtca aaaacttcaa ccgtagttgt caaattcgtc aactccagtt gtcaaattcg
      661 tcaactgagg ttgtcaaatc cgacaactct agccagtttc caagtagaaa ctacagtttc
      721 aaaactgcaa ctttttctac tttttgcaac ttaatctatt gactagtttt atataaatgt
      781 taaaacatat atatagaaat aaataaaaaa aggaggtttc tatggattta ggaaatatat
      841 taaatgagag tttaagtatt gattacgaaa aattagattt gtttttggaa aaatatgatt
      901 taacaccaga acaaaaagtt gcagtttatg aatttcacgc aaaagcttat aaaaaaaata
      961 aaactttagt tatttctgaa acaaaagaaa ataaatttaa atctatttcc gaaggtgttg
     1021 aatacgtgca tttattccca aaaaatttaa aaattttaat taaaaaatat ggtttaaata
     1081 caaacgaatt attggtttta acggaaataa tggagtcaat gctttcacac ggaaatttat
     1141 taattaattt ttcgcaaaag gcactttgcg aattaacagg aattaataaa tctacaatgt
     1201 gtaaaacatt taaaaccctc aaacaaaagc agtgtttaat tgagaaaaac ggacatattt
     1261 atttaaattc tgtgatattt atgaaagggt tacctcataa attgtttatg caatttagag
     1321 atcatttttt aaattctatc tcatataaat tagatgatga agaagaattt gaaaaagtct
     1381 tcgacgataa ttttattaaa gcatacgaaa aaaatctcaa agagattaaa aagaaaaagc
     1441 aacaaataaa agaaaagaaa atatcaaaag cattagataa ttttgaaaaa gaaatctccg
     1501 aaagaatgga aagaaaagtt taaagacgaa gaggaaaatt tcgaatttgg ttttgaatcg
     1561 gaaatataaa accgccctcg ccgggcaggc caatccctta ctgaaataga ataaattcta
     1621 ttccactaag ggattttttt attcattgtt tctccacatt tgcaatattg acattaactt
     1681 ccacccggat ataacagtag tataagttgt tgtttcaacc cgtctttttg ggtggaacaa
     1741 caaggcattt tatgggtaaa gcgaagcgaa cccgtaaaac tgccaccccc aaccgggggt
     1801 cgttgttcga ttttgagcga cagcgaaaaa atagaacata aggggggagg gtttgggttt
     1861 tacggtattt taaatatgag caaagcgaat ttttaaaatg tccggttctt taaattttgc
     1921 aatgaggaaa aactattatg ggtaattcaa aaagaaatat aaataaatta aatgaaaatt
     1981 ttagagagga tattttagat tatgcgatcg cgcacaatct aaaatgtgct aacgcactcg
     2041 ctattttata cgccaccggt tgccgtccgg acgaactcca cactggggtt aaaataaatt
     2101 acgataaaca aaaaaatgaa attagattta aaattattgg atcaaaactt aatcgaagaa
     2161 tgaaaagagg tattggaatt cgtgagtttt cagtaaaaat caataatgat aatgaaagat
     2221 ttttcaaagg aattattgat ctaataaatg aaagacctgt tgattcattc gatcataatt
     2281 ttaaaattga aagtgcaaag gcattttccg gatatattac aaaaa
//
