LOCUS       NZ_AP026430             2868 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645
            plasmid pKAM645_10, complete sequence.
ACCESSION   NZ_AP026430
VERSION     NZ_AP026430.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446298
            Assembly: GCF_024347815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 101x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:42:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,116
            CDSs (total)                      :: 6,009
            Genes (coding)                    :: 5,826
            CDSs (with protein)               :: 5,826
            Genes (RNA)                       :: 107
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 77 of 183
            Pseudo Genes (incomplete)         :: 115 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 35 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026430.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM645"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM645_10"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(2317..2868,1..129))
                     /locus_tag="OCV84_RS30510"
     CDS             complement(join(2317..2868,1..129))
                     /locus_tag="OCV84_RS30510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="WP_261902567.1"
                     /translation="MATKIENDEVIPDEELNKIIEEINNEEDKRLKVIVDEYQQLTQET
                     TDLVGEVVSFSLIDDKNNILINEYCKPTRNKISKTAQSIHNISNKMIKDSRNIIPIYED
                     FLKIINTEENKNKTIFIYNEGFDLSTIYRSLNYAGYKYKENNDFDDVEFRSRCVMKDFV
                     YFYGEHNEYYDTDKYKWKKLEFAANYFNIETKNLKLHNSAVDCYLTLKVIENIRDHFSG
                     RNYG"
                     /name="exonuclease domain-containing protein"
     gene            complement(511..1101)
                     /locus_tag="OCV84_RS30515"
                     /old_locus_tag="KAM645c_59450"
     CDS             complement(511..1101)
                     /locus_tag="OCV84_RS30515"
                     /old_locus_tag="KAM645c_59450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086642458.1"
                     /translation="MTKKNNVEILHKEKTNIENDSEIEHKKNPFVFNMSIDTKKKKLTV
                     DRGSEVETEEGVFTTTISQIQEVDREEFLKIFTGQIKFYFDLSQTGFKLFFIIMGLYQK
                     QIGGDEIYLNFETAKEEAEKYESAISKAVYYRGLKELLEKKMIAKSVKRYIFYINPAII
                     FNGDRARFVKEIKIKEEEKQQEENLKILRSKYA"
                     /name="hypothetical protein"
     gene            complement(1590..2324)
                     /locus_tag="OCV84_RS30520"
                     /old_locus_tag="KAM645c_59460"
     CDS             complement(1590..2324)
                     /locus_tag="OCV84_RS30520"
                     /old_locus_tag="KAM645c_59460"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_086642456.1"
                     /translation="MGNSKRNIKKLNDNFRESILEYAISHNLKCANALVALYATGCRPD
                     EIETGIKVNFDSKNNKLSFKIIGSKLNYQQKRGIRIREVTVKITDKNLQYYKSIIDIFE
                     KNPQAYDLKIKTESAKAFSGYITKISKKLWPRKKYHASAYSFRHAKATELKNSEGFDAE
                     KIAKIMGHASVRSQQSYGRKSRRSAGGFDDITDVETSSKPRGGDRLLRFKIKNKNEAAK
                     KIKAASTPTDTVSPPPVRSLKR"
                     /name="site-specific integrase"
ORIGIN
        1 ttgcgtaagt tgttgatatt catcaacaat tactttcaat cttttatctt cttcattatt
       61 tatctcttct attattttat ttaattcctc atctggaatt acttcgtcat tttcaatttt
      121 tgttgccata atttcacctc atttattgtt ttttatattg ttaacatcaa attccggaga
      181 tttcaaaatt tcgctttgct caaatttgaa ataccgtaaa acccaaacca tccccccatt
      241 atttaaattt ttcgcgttgc ttcaaaattc aaacaatgac ccccggttgg gggtggcata
      301 atttcgggta ataaaaaact tacccttcat aatgccttgt tgttccaccc caagaacggg
      361 gtggaaacaa caaaagataa tatagttata tccgggttga aattaatgtc aatatttgaa
      421 atgtggagaa aatgagaata aaaaaatccc ttagaaaata gaattttttt ctatttttta
      481 agggatttgg cccgtccggc gaggacggtt ttaagcatat tttgagcgaa gaatttttaa
      541 attttcttct tgttgttttt cttcttcttt aattttaatt tctttaacaa atcttgcacg
      601 atcgccattg aaaatgatcg ccgggttaat ataaaaaatg tatcttttta cagattttgc
      661 gatcattttc ttttctaata attcttttaa acctctatag tagacagctt ttgaaattgc
      721 tgactcgtat ttttcagctt cctcttttgc tgtttcgaaa tttaaataaa tttcgtcacc
      781 gcctatctgt ttttgataca aacccataat tatgaaaaac aatttaaatc cggtttgaga
      841 caaatcaaaa tagaatttga tttgtcctgt aaaaattttt aaaaattctt ccctatcaac
      901 ttcttgaatt tgtgatattg ttgttgtgaa aaccccctct tctgtttcta cttctgaacc
      961 ccgatcaaca gttagttttt tctttttagt atcaatagac atattgaaaa caaacgggtt
     1021 ctttttatgt tcgatctcac tatcgttttc aatattagtt ttttctttgt gtaaaatttc
     1081 tacattgttt ttctttgtca tatacaaacc ctctctgttt aaaaaataac cgaaaaaatt
     1141 ctcactcggt gagactcttc ttctcatttt ttaacagatt tttgagacta gtcaagaaaa
     1201 cacattggat ttattctcat tttttacaaa aaaaatgaga caaaaagtca caatatcgag
     1261 acatttagtc actgtagaga gaattttttc tctcctgaga gaattttttt tctctataga
     1321 gactagacat ccagtgttta tgcgggttac gagacatata agaatataag aaatataaga
     1381 tctaagaggc tttgcttagc tctgagtaaa atttttatcg gcattgtgat aactgggctt
     1441 cgcaatagaa aaccaaaaac catctggaaa accatcccag ttaccccgat tccggggccc
     1501 tgcttcgcaa tatggaggat tttgctataa aaaccgtcct ggccggacgg gccaaccctt
     1561 caaagccaca cagcaagctg tgtagcttct cagcgtttta gagaacgaac tggaggtgga
     1621 cttactgtat ccgtaggggt agaagctgct ttaattttct ttgctgcttc atttttgttc
     1681 ttaatcttaa atctcagcaa tctatcgccc ccacggggct tagagcttgt ttcaacatct
     1741 gtaatgtcgt caaatccacc tgcgcttctt ctgctctttc taccgtagct ttgttgtgat
     1801 ctgacagaag catgacccat aattttagcg attttttcag catcaaaacc ctctgaattt
     1861 ttcaattctg ttgcttttgc atgtctaaaa gaatacgccg aggcgtgata ttttttcctg
     1921 ggccatagtt ttttgcttat ttttgtaatg tatccggaaa acgcttttgc actttcagtt
     1981 ttaattttta aatcatatgc ctgagggttt ttctcaaaaa tatctattat agatttgtaa
     2041 tattgaagat ttttatctgt gatttttacc gtaacctctc taattctaat acctcttttt
     2101 tgttgataat ttaattttga gccgatgatt ttaaaactta acttattgtt tttactatca
     2161 aaattaactt taatgccagt ttcgatctcg tccggtctgc aacctgtggc atataatgcc
     2221 acaagtgcgt tagcgcattt tagattatgc gaaatcgcat attctaaaat actttctcta
     2281 aaattatcat ttagtttttt tatatttcgt tttgaattac ccataatttc tacctgaaaa
     2341 atgatctcta atgttttcaa ttacctttaa ggttaaataa caatcgacgg ctgaattatg
     2401 caattttaag tttttcgttt cgatattaaa ataatttgca gcaaattcga gctttttcca
     2461 tttgtattta tctgtatcat agtactcatt atgttcacca taaaaataca caaaatcttt
     2521 cattacgcaa cgagatctaa attctacatc gtcgaaatcg ttattttctt tatatttata
     2581 tcctgcataa tttaaagatc tatatattgt tgaaagatca aaaccttcat tataaataaa
     2641 aatagtttta tttttatttt cttctgtgtt tattattttc aaaaaatcct catatatagg
     2701 aattatattt ctactatctt tgatcatttt attagaaata ttatgtattg actgtgctgt
     2761 ttttgaaatc ttattcctgg ttggtttgca atattcgtta attaatatat tgtttttgtc
     2821 gtcaatcaat gaaaaactaa caacctcacc aacaagatct gttgtttc
//
