LOCUS       NZ_AP026428             4635 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM645
            plasmid pKAM645_8, complete sequence.
ACCESSION   NZ_AP026428
VERSION     NZ_AP026428.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446298
            Assembly: GCF_024347815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 101x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:42:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,116
            CDSs (total)                      :: 6,009
            Genes (coding)                    :: 5,826
            CDSs (with protein)               :: 5,826
            Genes (RNA)                       :: 107
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 77 of 183
            Pseudo Genes (incomplete)         :: 115 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 35 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026428.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM645"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM645_8"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(<240..335)
                     /locus_tag="OCV84_RS30460"
                     /pseudo=""
     CDS             complement(<240..335)
                     /locus_tag="OCV84_RS30460"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(<370..1050)
                     /locus_tag="OCV84_RS30630"
                     /pseudo=""
     CDS             complement(<370..1050)
                     /locus_tag="OCV84_RS30630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1013..1336)
                     /locus_tag="OCV84_RS30470"
                     /old_locus_tag="KAM645c_59390"
     CDS             complement(1013..1336)
                     /locus_tag="OCV84_RS30470"
                     /old_locus_tag="KAM645c_59390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_198661910.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAVWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1976..2134
                     /locus_tag="OCV84_RS30475"
                     /old_locus_tag="KAM645c_59400"
     CDS             1976..2134
                     /locus_tag="OCV84_RS30475"
                     /old_locus_tag="KAM645c_59400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_118998265.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAAIGNWELVDLDI
                     ESLEVMA"
                     /name="replication initiation protein"
     gene            complement(2377..3171)
                     /locus_tag="OCV84_RS30480"
     CDS             complement(2377..3171)
                     /locus_tag="OCV84_RS30480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_261902564.1"
                     /translation="MKNTAWNVLVYSHADSATTILITYAKSVLVLLIVVIIAFSLLSYF
                     IASRISIPLEKLAISTNTKDIGASLVYIKAINAWYAEADRLKKALFTHIKVMMERVDSL
                     NEVAVKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFFKKINDSFGHGVGDEVLVS
                     LAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVLTAECVAERIRSVIESTVFPNNLRVTV
                     SAGVATQDDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(3168..3932)
                     /locus_tag="OCV84_RS30485"
     CDS             complement(3168..3932)
                     /locus_tag="OCV84_RS30485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="WP_261902565.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIVVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMGNYIEMAQSELAYGAKKIESTNDIDVLKNEADRLRLQSGMFNSVLV
                     VSNGAVVLATSPESLGLVGVHLNSSASKLAIEGKKPFVSAPYKSATGNLIVLLSHPIYD
                     KNGNYIGYVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNNNSAVGRPMV
                     MSDDLKSKLTESDRGEGDFFLRGIDIFWVMLI"
                     /name="cache domain-containing protein"
     gene            complement(4286..4495)
                     /locus_tag="OCV84_RS30490"
                     /old_locus_tag="KAM645c_59420"
     CDS             complement(4286..4495)
                     /locus_tag="OCV84_RS30490"
                     /old_locus_tag="KAM645c_59420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_118998865.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLNEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1446..1625)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.363"
                     /coverage="83.0"
                     /evalue="3.48e-51"
     Typing          1572..2088
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.101"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5..1023)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.294"
                     /coverage="89.0"
                     /evalue="0.0"
     Typing          complement(4177..4634)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.503"
                     /coverage="99.0"
                     /evalue="3.72e-78"
ORIGIN
        1 tcccggatgg tcatgtaatt gtcgagtatc tgctgcccct gccaccacag aatgcccgcg
       61 ctcgaggcga tcagcagcgc ggagaccagc acgatggtca gccacgtctg gctgaccatg
      121 cgcagcatcc ctttcgttcg ctggctcatg gctgacgaca gcttccggtc gtggtcgagg
      181 atggcggcgc tgattctctt ctcgctttcg ctcagttccg ctctgacaga cttctcgtgc
      241 tcgctgaatg cggctttcag catctcgccg gtagtctgct gctgcgcttt cgatttctgc
      301 tctaagtcct tcgccagcgt taaaagactg ttcatagatg gctcccttca gtcggatgtt
      361 tcgccccccg tccgggtcgg caatgctgat gctgcttttt gtggtgcgca ccacttcaaa
      421 acccgcgctt tccagcgcct cagtgacgtc ctgacgcgtt ttcagctccc cggacgaggc
      481 taaggccagt aaaccccgcg tgatcgcctg agctgcctcc tgcttcgctt ccggcagcgc
      541 ggaaggcgtg accagcgccc gccggttttc cggcgcgttc gggtcgtgca gccccatcct
      601 gccgttcacg atggtctgcc aggcatcgat gcgcggacgg tcagcgcggt cgtaatacgg
      661 ctggagccgt ttgccggtca gcagctccgt gttcggtacc agaaaattaa gctccagacg
      721 gcccttgtcc tggtgttcaa cccacagcac gctgtactgg tctttatcga gtccgggcat
      781 cagaacccgc tcgaaactcg ccatcagcgt ttcacgctgg ccgggcggta agtcctgttc
      841 ggcaaaggac aggacgccgg aggtgtactt ttttgcataa ggcgaggcgt cgataagctc
      901 ccggacttcc tccggcttcc cctgcagaac gctggcacct tcgcgctggc ggtcttttcc
      961 cagcagataa tccaccggac cggcgccgcc gccacgtccc cgaggatgaa acttaacgat
     1021 catcgtctgc acccttttcc agcacggcat gcctcagccg ctcgagtccg gcatcgattg
     1081 ccatcagcgc ggcgacgacc tgtacccggt cgtgcccgct gccgccaccg gcgttaacct
     1141 gccgggcaat ctgattgagg ttgttcccca tgccggcaag ctgacgaagc agcgccggcg
     1201 agagcgacgg aagtttgccg gcacgagcgg gcttctcgtc caggcacgtc tgccgcatcc
     1261 agaccgcgag ctgcttaccg tcgcagcgct ccagcagacg gcggtgctca tcctcggtaa
     1321 cccacatcgt gagcattttg ctgcgtttat ctgccaatcc ggcctcctga taacgccggg
     1381 acgggcggga aatcttttcc cggtcggcgc ggcgttggac aagccgcagg cgcgtcagtg
     1441 gcttttagcg ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat
     1501 actggcttac tatgataaat cggagtggtg attcagggaa atgctttatg tagcaggggg
     1561 aaaatgcagc tgcggggcgt aagtgcaaaa tgcaggtggc gatgaaatga cgcctcctcg
     1621 ctcactcggt cgctgcgctc ctgccgtgag actgcggcgg gcgttacgga cttacaaata
     1681 atgggagagc agatggcgag aagtcaggaa gaccagaaga aggatgcggc gttgccgttt
     1741 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggcgg
     1801 cgaaacccga caggactata aagatcccag gcgtttcccc ctggtagctc cctcgtgcgc
     1861 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     1921 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     1981 cgaacccccc gttcagtccg actgctgcgc tttatccggt aactatcgtc ttgagtccaa
     2041 cccggaaaga cacgccaaag cgccactggc agcagccatt ggtaactggg aattagtgga
     2101 tttagatatc gagagtcttg aagtgatggc ctgactacgg ctacactgaa aggacagtat
     2161 ttggtatctg cgctccactt aagccagtta ccttcggaag aaagagttgg tagctctgaa
     2221 ttccggcaaa cgaaccaccg ttggtagcgg tggttttttt gtttacaggg catgagctta
     2281 cgacgacatc gaagggataa caagaggatc cgcttcttga accctcttcc ccacaaaaaa
     2341 aacgacacac tagcgcgtaa agcgtcatca aaaaactcaa atagcgctat acacaatcac
     2401 tttattacgc ccctctcctt tggcttgata aagagcatcg tcagcatttt tcagcaaaaa
     2461 atcgatccca ccaagaggat catcttgtgt tgccacacca gcagatacgg tgacccgcaa
     2521 gttattaggg aaaacagtac tttcaataac actcctaata cgctccgcaa cacactctgc
     2581 tgttaacaca gatgtattcg gcagaaaaac aacaaactct tcgccaccaa accgacaaat
     2641 aatatcttca ctacggcaac aagacttaat aatctgagcc aaagaaacaa gcacctcatc
     2701 tccgacccca tgcccgaaac tatcatttat ttttttaaaa aaatcaacat caatagcaat
     2761 aactgaagct cctgccccat acttgttatt ctgtatttta gttgaaaatc ccaatctatt
     2821 gttcacgccg gtaaggggat ctttaaccgc cacctcattt aaagaatcaa ccctttccat
     2881 cattacctta atatgcgtaa aaagtgcctt tttaagtcga tcagcttcgg cataccaagc
     2941 attgatagct ttaatataaa ctagcgaagc tcctatatct ttagtattgg tagatatagc
     3001 aagcttttct aaaggaattg aaatacggga ggcaatgaaa tatgaaagaa gtgaaaatgc
     3061 aataattact acaattagca gaacaagtac acttttagca taagtaataa gaatagtagt
     3121 cgcgctgtca gcatgactat aaaccaacac attccaagca gtatttttca tatgagcata
     3181 acccaaaaga tatctattcc ccgaagaaaa aagtccccct cacctctatc agactcagtt
     3241 aactttgatt tcaaatcatc agacataacc atgggccgac caacagcact gttattgtta
     3301 ttaaagatca cagttccatc atcactaaca atgctaattt cagtgtcgct cttgaaaaaa
     3361 tgcctgctca aaacatcgct aaacaaacta tgtttcttta gatatataga cccgccgaca
     3421 taccctatgt aattgccatt cttatcataa ataggatgtg acaacaaaac aatcaaattc
     3481 ccagtagctg atttgtaagg tgccgatacg aatggttttt tgccctcaat cgccaatttg
     3541 ctggcacttg aattcaagtg gacaccaact aaaccaagtg attctggcga agtcgccaga
     3601 acaacagctc cattactaac aacaagaact gaattaaaca taccagactg caatcttaac
     3661 ctatccgcct catttttaag tacatcaatg tcattagttg attctatttt ttttgcacca
     3721 tatgccaatt cactttgagc catttcaata taattaccca ttacatctga cattttcata
     3781 gcgtacgatg aattagcatc aagcaagtct ttttctatat tttcgcgctg aaaaaccata
     3841 ataacgacaa tcagaaaaat agaagtcaac aaaatcccac caacggataa ggttatgact
     3901 atcggacgaa ggtgaggttt tttaattctc ataaaaatac actccatata attaaaaaat
     3961 gccgcattta ccataacatc ccttaccggc accgcgccgg gcaggttcgg cgcgaatagg
     4021 gtgcatgccg ggtaaaacag acagtaaacc atcccgaaaa ccgcgcattc tgcacctgat
     4081 aaatttcacc ggattgcata gccatgcagc agggtgaatg gcaccggctt tcggcgcaga
     4141 tacccgccgg aagcctcgtt ttatccgaat tgttacagcg acggtccatt gtatgtccgc
     4201 tgccgctgcg atcgttcaag ctccagcgct ttctgatgct gcgccgcgcg ctcctgctcc
     4261 cggtgtgctt caatgccgtc cagtctcatg acaaccggcg cagctgctcg ttcaaactct
     4321 gcacctgcct gctcaagccc gtgacgcgct cgctcagcgc cgcgttctcc cgttgcataa
     4381 gaccagacat cgtccgccat tccgcgaagg cgttctccca ctcgtccagc cttttcgagt
     4441 agtcctgctg tagctgctct aatgcgctca gcaactgttt ttccagctcc gtcatgtgct
     4501 atttccccgc cagtatcatc cagctcgaat cctctccgaa cgtcccccct tccgggttca
     4561 ccctcacgca gcggcgtctc tgctctccgc aggtcgtgag ctgcacgccg ctgttcctct
     4621 cggacagcat ggcct
//
