LOCUS       NZ_AP026418             2322 bp    DNA     circular CON 16-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644
            plasmid pKAM644_11, complete sequence.
ACCESSION   NZ_AP026418
VERSION     NZ_AP026418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446297
            Assembly: GCF_024347795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 189x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347795.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 09:13:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,149
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 99 of 213
            Pseudo Genes (incomplete)         :: 134 of 213
            Pseudo Genes (internal stop)      :: 36 of 213
            Pseudo Genes (multiple problems)  :: 46 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026418.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM644"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM644_11"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(1821..2322,1..215))
                     /locus_tag="P4I75_RS30695"
     CDS             complement(join(1821..2322,1..215))
                     /locus_tag="P4I75_RS30695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_276097518.1"
                     /translation="MDLGNILNESLSIDYEKLDLFLEKYDLTPEQKVAVYEFHAKAYKK
                     NKTLVISETKENKFKSISEGVEYVHLLYGLNTNELLVLTEIMESMLSHGNLLINFSQKA
                     LCELTGINKSTMCKTFKTLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFMQFRDHFLNSI
                     SYKLDDDEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFEKEISKEWKEK
                     FKDEEENFEFGFESEI"
                     /name="hypothetical protein"
     gene            complement(721..1452)
                     /locus_tag="P4I75_RS30700"
                     /old_locus_tag="KAM644c_59760"
     CDS             complement(721..1452)
                     /locus_tag="P4I75_RS30700"
                     /old_locus_tag="KAM644c_59760"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_088299203.1"
                     /translation="MGNSKRNINKLNENFREDILDYAIAHNLKCANALAILYATGCRPD
                     ELHTGVKINYDKQKNEIRFKIIGSKLNRRMKRGIGIREFSVKINNDNERFFKGIIDLIN
                     ERPVDSFDHNFKIESAKAFSGYITKISKKLWPRKTYHASAYSFRHAKATELKNSDYDKE
                     KIAQIMGHASVRSQQSYGRKNKKSKGGFNDIEDIKTNVKPRGADRLLRFKIASKNQAAA
                     KIADTSTPSSPPPAPVRRFKM"
                     /name="site-specific integrase"
     Typing          complement(477..661)
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="1.6600000000000002e-73"
ORIGIN
        1 aataaatgca cgtattcaac accttcggaa atagatttaa atttattttc ttttgtttca
       61 gaaataacta aagttttatt ttttttataa gcttttgcgt gaaattcata aactgcaact
      121 ttttgttctg gtgttaaatc atatttttcc aaaaacaaat ctaatttttc gtaatcaata
      181 cttaaactct catttaatat atttcctaaa tccatagaaa cctccttttt ttatttattt
      241 ctatatatat gttttaacat ttatataaaa ctagtcaata gattaagttg caaaaagtag
      301 aaaaagttgc agttttgaaa ctgtagtttc tacttggaaa ctggctagag ttgtcggatt
      361 tgacaacctc agttgacgaa tttgacaact ggagttgacg aatttgacaa ctacggttga
      421 agtttttgac aactgccttt tttaaaaagt cagttagatc aatggttgga gagtagtttt
      481 taaaccccta agaacatcta agaattttaa gaacactaag ggggtgaaat tttcaacttt
      541 tgagatggtt tttaagatca ttggttttca agtaacagat caatccccca caggggaaaa
      601 ccatccagtt acccggttcc gggccctgct tcgcaatatg gaggattttg ctagaaaacc
      661 ggctcgccgc caggccaacc cttcacagtc caggagggga cctcctgaac tgctcacggt
      721 tcacatttta aagcgacgaa cgggagccgg tggaggactg ctgggggtgg aagtatcggc
      781 aatttttgcc gctgcttggt ttttgctggc aattttgaac cttagcaatc tatctgcacc
      841 acggggttta acattagttt ttatatcttc aatgtcgtta aacccacctt tgcttttttt
      901 gttttttcta ccgtaacttt gctgagatct aactgacgca tggcccatta tttgggctat
      961 tttttcttta tcataatctg aatttttcaa ctcagttgct tttgcatgcc tgaaagaata
     1021 tgcagaagca tgataagttt ttcggggcca tagctttttg cttatttttg taatatatcc
     1081 ggaaaatgcc tttgcacttt caattttaaa attatgatcg aatgaatcaa caggtctttc
     1141 atttattaga tcaataattc ctttgaaaaa tctttcatta tcattattga tttttactga
     1201 aaactcacga attccaatac ctcttttcat tcttcgatta agttttgatc caataatttt
     1261 aaatctaatt tcattttttt gtttatcgta atttatttta accccagtgt ggagttcgtc
     1321 cggacggcaa ccggtggcgt ataaaatagc gagtgcgtta gcacatttta gattgtgcgc
     1381 gatcgcataa tctaaaatat cctctctaaa attttcattt aatttattta tatttctttt
     1441 tgaattaccc ataatagttt ttcctcattg caaaatttaa agaaccggac attttaaaaa
     1501 ttcgctttgc tcatatttaa aataccgtaa aacccaaacc ctcccccctt atgttctatt
     1561 ttttcgctgt cgctcaaaat cgaacaacga cccccggttg ggggtggcag ttttacgggt
     1621 tcgcttcgct ttacccataa aatgccttgt tgttccaccc aaaaagacgg gttgaaacaa
     1681 caacttatac tactgttata tccgggtgga agttaatgtc aatattgcaa atgtggagaa
     1741 acaatgaata aaaaaatccc ttagtggaat agaatttatt ctatttcagt aagggattgg
     1801 cctgcccggc gagggcggtt ttatatttcc gattcaaaac caaattcgaa attttcctct
     1861 tcgtctttaa acttttcttt ccattctttc gagatttctt tttcaaaatt atctaatgct
     1921 tttgatattt tcttttcttt tatttgttgc tttttctttt taatctcttt gagatttttt
     1981 tcgtatgctt taataaaatt atcgtcgaag actttttcaa attcttcatc atcatctaat
     2041 ttatatgaga tagaatttaa aaaatgatct ctaaattgca taaacaattt atgaggtaac
     2101 cctttcataa atatcacaga atttaaataa atatgtccgt ttttctcaat taaacactgc
     2161 ttttgtttga gggttttaaa tgttttacac attgtagatt tattaattcc tgttaattcg
     2221 caaagtgcct tttgcgaaaa attaattaat aaatttccgt gtgaaagcat tgactccatt
     2281 atttccgtta aaaccaataa ttcgtttgta tttaaaccat at
//
