LOCUS       NZ_AP026417             2889 bp    DNA     circular CON 16-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM644
            plasmid pKAM644_10, complete sequence.
ACCESSION   NZ_AP026417
VERSION     NZ_AP026417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00446297
            Assembly: GCF_024347795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Complete genome sequence of carbapenem-resistant Klebsiella spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2022) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 189x
            Sequencing Technology             :: Illumina HiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024347795.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 09:13:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,149
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 99 of 213
            Pseudo Genes (incomplete)         :: 134 of 213
            Pseudo Genes (internal stop)      :: 36 of 213
            Pseudo Genes (multiple problems)  :: 46 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026417.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KAM644"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKAM644_10"
                     /geo_loc_name="Japan"
                     /collection_date="2020"
     gene            complement(join(2642..2889,1..454))
                     /locus_tag="P4I75_RS30680"
     CDS             complement(join(2642..2889,1..454))
                     /locus_tag="P4I75_RS30680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]; GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="WP_276097517.1"
                     /translation="MATKIENDEVIPDEELNKIIEEINNEEDKRLKVIVDEYQQLTQNG
                     FFVLDTETTDLVGEVVSFSLIDDKNNILINEYCKPTRNKISKTAQSIHNISNKMIKDSR
                     NIIPIYEDFLKIINTEENKNKTIFIYNEGFDLSTIYRSLNYAGYKYKDNDFDDVEFRSR
                     CVMKDFAYFYGEHNEYYDTDKYKWKKLEFAANYFNIETKNLKLHNSAVDCYLTLKVIEN
                     IRDHFSGRNYG"
                     /name="3'-5' exonuclease"
     gene            complement(836..1426)
                     /locus_tag="P4I75_RS30685"
                     /old_locus_tag="KAM644c_59740"
     CDS             complement(836..1426)
                     /locus_tag="P4I75_RS30685"
                     /old_locus_tag="KAM644c_59740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086642458.1"
                     /translation="MTKKNNVEILHKEKTNIENDSEIEHKKNPFVFNMSIDTKKKKLTV
                     DRGSEVETEEGVFTTTISQIQEVDREEFLKIFTGQIKFYFDLSQTGFKLFFIIMGLYQK
                     QIGGDEIYLNFETAKEEAEKYESAISKAVYYRGLKELLEKKMIAKSVKRYIFYINPAII
                     FNGDRARFVKEIKIKEEEKQQEENLKILRSKYA"
                     /name="hypothetical protein"
     gene            complement(1915..2649)
                     /locus_tag="P4I75_RS30690"
                     /old_locus_tag="KAM644c_59750"
     CDS             complement(1915..2649)
                     /locus_tag="P4I75_RS30690"
                     /old_locus_tag="KAM644c_59750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_086642456.1"
                     /translation="MGNSKRNIKKLNDNFRESILEYAISHNLKCANALVALYATGCRPD
                     EIETGIKVNFDSKNNKLSFKIIGSKLNYQQKRGIRIREVTVKITDKNLQYYKSIIDIFE
                     KNPQAYDLKIKTESAKAFSGYITKISKKLWPRKKYHASAYSFRHAKATELKNSEGFDAE
                     KIAKIMGHASVRSQQSYGRKSRRSAGGFDDITDVETSSKPRGGDRLLRFKIKNKNEAAK
                     KIKAASTPTDTVSPPPVRSLKR"
                     /name="site-specific integrase"
ORIGIN
        1 ctttatattt atatcctgca taatttaaag atctatatat tgttgaaaga tcaaaacctt
       61 cattataaat aaaaatagtt ttatttttat tttcttctgt gtttattatt ttcaaaaaat
      121 cctcatatat aggaattata tttctactat ctttgatcat tttattagaa atattatgta
      181 ttgactgtgc tgtttttgaa atcttattcc tggttggttt gcaatattcg ttaattaata
      241 tattgttttt gtcgtcaatc aatgaaaaac taacaacctc accaacaaga tctgttgttt
      301 ctgtatcaag tacgaaaaaa ccgttttgcg taagttgttg atattcatca acaattactt
      361 tcaatctttt atcttcttca ttatttatct cttctattat tttatttaat tcctcatctg
      421 gaattacttc gtcattttca atttttgttg ccataatttc acctcattta ttgtttttta
      481 tattgttaac atcaaattcc ggagatttca aaatttcgct ttgctcaaat ttgaaatacc
      541 gtaaaaccca aaccatcccc ccattattta aatttttcgc gttgcttcaa aattcaaaca
      601 atgacccccg gttgggggtg gcataatttc gggtaataaa aaacttaccc ttcataatgc
      661 cttgttgttc caccccaaga acggggtgga aacaacaaaa gataatatag ttatatccgg
      721 gttgaaatta atgtcaatat ttgaaatgtg gagaaaatga gaataaaaaa atcccttaga
      781 aaatagaatt tttttctatt ttttaaggga tttggcccgt ccggcgagga cggttttaag
      841 catattttga gcgaagaatt tttaaatttt cttcttgttg tttttcttct tctttaattt
      901 taatttcttt aacaaatctt gcacgatcgc cattgaaaat gatcgccggg ttaatataaa
      961 aaatgtatct ttttacagat tttgcgatca ttttcttttc taataattct tttaaacctc
     1021 tatagtagac agcttttgaa attgctgact cgtatttttc agcttcctct tttgctgttt
     1081 cgaaatttaa ataaatttcg tcaccgccta tctgtttttg atacaaaccc ataattatga
     1141 aaaacaattt aaatccggtt tgagacaaat caaaatagaa tttgatttgt cctgtaaaaa
     1201 tttttaaaaa ttcttcccta tcaacttctt gaatttgtga tattgttgtt gtgaaaaccc
     1261 cctcttctgt ttctacttct gaaccccgat caacagttag ttttttcttt ttagtatcaa
     1321 tagacatatt gaaaacaaac gggttctttt tatgttcgat ctcactatcg ttttcaatat
     1381 tagttttttc tttgtgtaaa atttctacat tgtttttctt tgtcatatac aaaccctctc
     1441 tgtttaaaaa ataaccgaaa aaattctcac tcggtgagac tcttcttctc attttttaac
     1501 agatttttga gactagtcaa gaaaacacat tggatttatt ctcatttttt acaaaaaaaa
     1561 tgagacaaaa agtcacaata tcgagacatt tagtcactgt agagagaatt ttttctctcc
     1621 tgagagaatt ttttttctct atagagacta gacatccagt gtttatgcgg gttacgagac
     1681 atataagaat ataagaaata taagatctaa gaggctttgc ttagctctga gtaaaatttt
     1741 tatcggcatt gtgataactg ggcttcgcaa tagaaaacca aaaaccatct ggaaaaccat
     1801 cccagttacc ccgattccgg ggccctgctt cgcaatatgg aggattttgc tataaaaacc
     1861 gtcctggccg gacgggccaa cccttcaaag ccacacagca agctgtgtag cttctcagcg
     1921 ttttagagaa cgaactggag gtggacttac tgtatccgta ggggtagaag ctgctttaat
     1981 tttctttgct gcttcatttt tgttcttaat cttaaatctc agcaatctat cgcccccacg
     2041 gggcttagag cttgtttcaa catctgtaat gtcgtcaaat ccacctgcgc ttcttctgct
     2101 ctttctaccg tagctttgtt gtgatctgac agaagcatga cccataattt tagcgatttt
     2161 ttcagcatca aaaccctctg aatttttcaa ttctgttgct tttgcatgtc taaaagaata
     2221 cgccgaggcg tgatattttt tcctgggcca tagttttttg cttatttttg taatgtatcc
     2281 ggaaaacgct tttgcacttt cagttttaat ttttaaatca tatgcctgag ggtttttctc
     2341 aaaaatatct attatagatt tgtaatattg aagattttta tctgtgattt ttaccgtaac
     2401 ctctctaatt ctaatacctc ttttttgttg ataatttaat tttgagccga tgattttaaa
     2461 acttaactta ttgtttttac tatcaaaatt aactttaatg ccagtttcga tctcgtccgg
     2521 tctgcaacct gtggcatata atgccacaag tgcgttagcg cattttagat tatgcgaaat
     2581 cgcatattct aaaatacttt ctctaaaatt atcatttagt ttttttatat ttcgttttga
     2641 attacccata atttctacct gaaaaatgat ctctaatgtt ttcaattacc tttaaggtta
     2701 aataacaatc gacggctgaa ttatgcaatt ttaagttttt cgtttcgata ttaaaataat
     2761 ttgcagcaaa ttcgagcttt ttccatttgt atttatctgt atcatagtac tcattatgtt
     2821 caccataaaa atacgcaaaa tctttcatta cgcaacgaga tctaaattct acatcgtcga
     2881 aatcgttat
//
